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1. 发明申请

WO2016030358A1 METHOD FOR TREATMENT OF TOOLS AND TOOLS USED FOR ISOLATION OF MICROVESICLES, NANOVESICLES OR EXSOMES 审中-公开
标题翻译：用于分离用于分离微胶囊，纳米颗粒或异物的工具和工具的方法
公开(公告)号：WO2016030358A1
公开(公告)日：2016-03-03
申请号：PCT/EP2015/069420
申请日：2015-08-25
申请人： JOZEF STEFAN INSTITUTE , UNIVERZA V LJUBLJANI
发明人： JUNKAR, Ita , MOZETIC, Miran , ZAPLOTNIK, Rok , KRALJ-IGLIC, Veronika , ŠTUKELJ, Roman
IPC分类号： A61L29/14 , A61L33/00 , A61M1/02 , A61M5/14 , B05D3/14
CPC分类号： A61L29/14 , A61L33/0094 , A61L2400/18
摘要： According to the present invention a method which prevents excessive adsorption of microvesicles on the surfaces of tools used for sampling, storing and handling body flu- ids containing microvesicles is provided. The method comprises the steps of: selecting said a tool from the a list plurality of tools including but not limited to needles, blood tubing, blood bags, catheters, Eppendorf tubes, pipettes or the like, providing said tool from said plurality of tools, providing a source of positively and negatively charged particles of high density, selecting a source assuring for formation of positively and negatively charged particles of high density and treating a surface of said tool by applying short pulses of said source of particles next to or on the said surface of said tool to assure surface modification of said surface by reacting said positively and negatively charged particles of high density on said surface. The method according to the present invention ensures contacting of tools with short pulses of highly ionized gas comprising both positively and negatively charged particles, the pulses being essentially short enough to avoid excessive heating of materials used for collecting, sampling, storage, transport and isolation of micro vesicles and the density of both positively and negatively charged particles which is essentially high enough to cause roughening of said tools on sub-micrometer or nanometer scale. Especially tools treated according to the present inventive method prevents excessive adsorption of microvesicles on the surfaces of said tools used for collecting, sampling, storing, transporting and isolating of microvesicles or the like. The method according to the present invention enables higher yields and lower frag- mentation of microvesicles for instance by preventing adsorption of this valuable diag- nostic material on the surface of different tools used for isolation and detection. Accordingly, the present invention also provides increasing the roughness of a material by the method according to the present invention. In particular the material which is used to produce diagnostic or medical tools or devices. (Fig. 1)
摘要翻译：根据本发明，提供了防止微泡在用于取样，储存和处理含有微泡的体液的工具的表面上过度吸附的方法。该方法包括以下步骤：从列表中选择多个工具的工具，包括但不限于针，血管，血袋，导管，Eppendorf管，移液管等，从所述多个工具提供所述工具，提供具有高密度的正带负电粒子源，选择源确保形成具有高密度的带正电荷和带负电荷的颗粒并通过施加所述工具的表面的所述短脉冲来施加所述工具的表面的所述短脉冲，所述工具的表面以通过在所述表面上反应所述带正电荷和带负电荷的高密度颗粒来确保所述表面的表面改性。根据本发明的方法确保工具与包括正带电荷和带负电粒子的短电离气体的短脉冲接触，所述脉冲基本足够短以避免过度加热用于收集，取样，储存，运输和分离的材料微泡和正和负带电粒子的密度，其基本上足够高以在亚微米或纳米级上引起所述工具的粗糙化。特别是根据本发明方法处理的工具防止微囊在用于收集，取样，储存，运输和分离微泡等的所述工具的表面上过度吸附。根据本发明的方法，通过防止这种有价值的诊断材料在用于分离和检测的不同工具的表面上的吸附，能够实现更高的产率和更低的微泡分解。因此，本发明还通过根据本发明的方法提高材料的粗糙度。特别是用于生产诊断或医疗工具或设备的材料。（图。1）

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