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1. 发明授权

US5532027A UV light treatment of clear coat to improve acid etch resistance 失效
标题翻译：紫外线处理透明涂层，提高耐酸蚀性
公开(公告)号：US5532027A
公开(公告)日：1996-07-02
申请号：US360557
申请日：1994-12-21
申请人： J. David Nordstrom , Hisanori Omura , Alan E. Smith , David M. Thomson
发明人： J. David Nordstrom , Hisanori Omura , Alan E. Smith , David M. Thomson
IPC分类号： B05D3/06 , B05D7/00
CPC分类号： B05D7/53 , B05D3/065
摘要： An improved process which comprises applying a layer of a color coating composition to a substrate used for the exterior of a motor vehicle and then applying a layer of a clear coating composition to the color coating and curing the resulting clear coat/color coat layer; the improvement is the use of a clear coating composition containing a film forming binder of an acrylosilane polymer and exposing the clear coat layer after curing to an artificial source of UV light under ambient temperatures and atmospheric conditions in an amount sufficient to improve the resistance of the clear coat to water spotting and acid etching when exposed to natural weathering conditions.
摘要翻译：一种改进的方法，其包括将一层彩色涂层组合物施加到用于机动车辆外部的基底上，然后将一层透明涂层组合物施加到彩色涂层上并固化所得到的透明涂层/彩色涂层; 改进之处在于使用含有丙烯硅烷聚合物的成膜粘合剂的透明涂料组合物，并且在环境温度和大气条件下将固化后的透明涂层暴露于人造紫外光源，其量足以提高当暴露于自然风化条件下，透明涂层对水斑点和酸蚀刻。

2. 发明授权

US07015026B2 Purification of adenovirus and AAV 失效
公开(公告)号：US07015026B2
公开(公告)日：2006-03-21
申请号：US10470604
申请日：2004-03-02
申请人： Catherine E. O'Riordan , Amy L. Helgerson , Alan E. Smith
发明人： Catherine E. O'Riordan , Amy L. Helgerson , Alan E. Smith
IPC分类号： C12N7/02
CPC分类号： C12N7/00 , C12N15/86 , C12N2710/10343 , C12N2710/10351 , C12N2750/14143 , C12N2750/14151 , Y10S435/803
摘要： The present invention relates to the purification of large scale quantities of active (infectious) adenovirus and AAV, especially for use in therapeutic applications. In particular, the invention provides improved methods for contacting such viruses with suitable chromatographic materials in a fashion such that any damage to the virus, particularly to surface components thereof, resulting from contact with such chromatographic materials is minimized or eliminated. The result is the ability to rapidly and efficiently purify commercial level quantities of active (infectious) virus suitable for use in therapeutic applications, e.g. gene transfer/therapy procedures.

3. 发明授权

US06569426B2 Tresyl-monomethoxypolyethylene glycol-modified viruses having viral infectivity 失效
标题翻译：具有病毒感染性的甲磺酸单甲氧基聚乙二醇修饰病毒
公开(公告)号：US06569426B2
公开(公告)日：2003-05-27
申请号：US09409803
申请日：1999-09-30
申请人： Alan E. Smith , Catherine R. O'Riordan , Gillian E. Francis , Vincent Parkes , Christina Delgado
发明人： Alan E. Smith , Catherine R. O'Riordan , Gillian E. Francis , Vincent Parkes , Christina Delgado
IPC分类号： A01N6300
CPC分类号： C12N15/86 , A61K47/6901 , A61K48/00 , C12N2710/10343 , C12N2810/10 , Y10S530/81
摘要： Viruses are modified by coupling a polymer such as polyethylene glycol to obtain polymer-modified viruses that can exhibit reduced antigenicity while retaining infectivity, and which may exhibit increased circulation time in vivo. The polymer may be directly covalently attached or indirectly covalently attached via an intermediate coupling moiety to the virus. The polymer may also be indirectly noncovalently attached to the virus via a ligand such as an antibody having specificity for a viral surface component. To prepare the polymer-modified virus, the polymer is activated and coupled to the virus. A preferred activated polymer is tresyl-monomethoxypolyethylene glycol having an average molecular weight of about 5000 daltons. The polymer-modified viruses have utility for therapeutic and diagnostic in vivo applications, and may be used to introduce a transgene into a target cell by infection, or be administered to a subject having a tumor where the polymer-modified virus localizes to the tumor.
摘要翻译：通过将聚合物例如聚乙二醇偶联来改进病毒以获得聚合物修饰的病毒，其可以在保留感染性的同时表现出降低的抗原性，并且其可以在体内表现出增加的循环时间。聚合物可以直接共价连接或间接地通过中间偶联部分共价连接到病毒上。聚合物还可以通过配体例如对病毒表面组分具有特异性的抗体非共价连接到病毒。为了制备聚合物改性的病毒，聚合物被激活并与病毒偶联。优选的活化聚合物是平均分子量为约5000道尔顿的甲磺酰单甲氧基聚乙二醇。聚合物修饰的病毒可用于治疗和诊断体内应用，并且可用于通过感染将转基因导入靶细胞，或被施用于具有聚合物修饰的病毒定位于肿瘤的肿瘤的受试者。

4. 发明授权

US6143548A Chromatographic purification of adeno-associated virus (AAV) 失效
标题翻译：腺相关病毒（AAV）的色谱纯化
公开(公告)号：US6143548A
公开(公告)日：2000-11-07
申请号：US11828
申请日：1998-06-29
申请人： Catherine E. O'Riordan , Amy E. Erickson , Alan E. Smith
发明人： Catherine E. O'Riordan , Amy E. Erickson , Alan E. Smith
IPC分类号： C12N15/09 , C12N7/02 , C12N15/864 , A23J1/00
CPC分类号： C12N7/00 , C12N15/86 , C12N2710/10343 , C12N2710/10351 , C12N2750/14143 , C12N2750/14151 , Y10S435/803
摘要： The present invention relates to the purification of large scale quantities of active (infectious) adenovirus and AAV, especially for use in therapeutic applications. In particular, the invention provides improved methods for contacting such viruses with suitable chromatographic materials in a fashion such that any damage to the virus, particularly to surface components thereof, resulting from contact with such chromatographic materials is minimized or eliminated. The result is the ability to rapidly and efficiently purify commercial level quantities of active (infectious) virus suitable for use in therapeutic applications, e.g. gene transfer/therapy procedures.
摘要翻译： PCT No.PCT / US96 / 13872 Sec。 371日期1998年6月29日第 102（e）1998年6月29日PCT PCT 1996年8月30日PCT公布。公开号WO97 / 08298 日期1997年3月6日本发明涉及大规模量的活性（感染性）腺病毒和AAV的纯化，特别是用于治疗应用。特别地，本发明提供了使这种病毒与合适的色谱材料接触的改进方法，使得与这种色谱材料的接触导致的对病毒，特别是其表面组分的任何损害被最小化或消除。其结果是能够快速有效地纯化商业级数量的适用于治疗应用的活性（感染性）病毒，例如，基因转移/治疗程序。

5. 发明授权

US07579181B2 Purification of adenovirus and AAV 失效
标题翻译：腺病毒和AAV的纯化
公开(公告)号：US07579181B2
公开(公告)日：2009-08-25
申请号：US11179865
申请日：2005-07-12
申请人： Catherine E. O'Riordan , Amy L. (Ericson) Helgerson , Alan E. Smith
发明人： Catherine E. O'Riordan , Amy L. (Ericson) Helgerson , Alan E. Smith
IPC分类号： C12N7/02
CPC分类号： C12N7/00 , C12N15/86 , C12N2710/10343 , C12N2710/10351 , C12N2750/14143 , C12N2750/14151 , Y10S435/803
摘要： The present invention relates to the purification of large scale quantities of active (infectious) adenovirus and AAV, especially for use in therapeutic applications. In particular, the invention provides improved methods for contacting such viruses with suitable chromatographic materials in a fashion such that any damage to the virus, particularly to surface components thereof, resulting from contact with such chromatographic materials is minimized or eliminated. The result is the ability to rapidly and efficiently purify commercial level quantities of active (infectious) virus suitable for use in therapeutic applications, e.g. gene transfer/therapy procedures.
摘要翻译：本发明涉及大规模量的活性（感染性）腺病毒和AAV的纯化，特别是用于治疗应用。特别地，本发明提供了使这种病毒与合适的色谱材料接触的改进方法，使得与这种色谱材料的接触导致的对病毒，特别是其表面组分的任何损害被最小化或消除。其结果是能够快速有效地纯化商业级数量的适用于治疗应用的活性（感染性）病毒，例如，基因转移/治疗程序。

6. 发明授权

US07318919B2 Adenovirus vectors for gene therapy 有权
标题翻译：用于基因治疗的腺病毒载体
公开(公告)号：US07318919B2
公开(公告)日：2008-01-15
申请号：US10161539
申请日：2002-06-03
申请人： Richard Gregory , Donna Armentano , Larry A. Couture , Alan E. Smith
发明人： Richard Gregory , Donna Armentano , Larry A. Couture , Alan E. Smith
IPC分类号： A61K48/00 , C12N15/861
CPC分类号： C12N15/86 , A61K48/00 , C12N2710/10343
摘要： Gene Therapy vectors, which are especially useful for cystic fibrosis, and methods for using the vectors are disclosed.
摘要翻译：公开了对囊性纤维化特别有用的基因治疗载体和使用载体的方法。

7. 发明授权

US5882877A Adenoviral vectors for gene therapy containing deletions in the adenoviral genome 失效
标题翻译：用于在腺病毒基因组中含有缺失的基因治疗的腺病毒载体
公开(公告)号：US5882877A
公开(公告)日：1999-03-16
申请号：US895194
申请日：1997-07-16
申请人： Richard J. Gregory , Donna Armentano , Larry A. Couture , Alan E. Smith
发明人： Richard J. Gregory , Donna Armentano , Larry A. Couture , Alan E. Smith
IPC分类号： A61K38/00 , A61K48/00 , C07K14/47 , C12N15/861 , C12N15/00 , C12N15/86
CPC分类号： C12N15/86 , A61K48/00 , C07K14/4712 , A61K38/00 , C12N2710/10343 , C12N2830/42 , C12N2840/203
摘要： Adenoviral vectors which contain deletions of the early regions and/or late genes provide efficient delivery and expression of foreign nucleic acids of interest to patients. These vectors have a particular use in the treatment of cystic fibrosis patients. Furthermore, PAV vectors provide for a second generation of adenoviral vectors that contain the 5' ITR's, the packaging signal and the E1A enhancer. Other adenoviral vectors contain a deletion of the E1 region or a deletion of E4 but retain orf3 or orf6, and can either retain or delete the E3 region.
摘要翻译：含有早期区域和/或晚期基因缺失的腺病毒载体提供对患者感兴趣的外来核酸的有效递送和表达。这些载体在囊性纤维化患者的治疗中具有特别的用途。此外，PAV载体提供含有5'ITR，包装信号和E1A增强子的第二代腺病毒载体。其他腺病毒载体含有E1区的缺失或E4的缺失，但保留orf3或orf6，并且可以保留或删除E3区。

8. 发明授权

US06472584B1 Production of membrane proteins in the milk of transgenic mammals 失效
标题翻译：在转基因哺乳动物的乳中生产膜蛋白
公开(公告)号：US06472584B1
公开(公告)日：2002-10-29
申请号：US09627290
申请日：2000-07-28
申请人： Alan E. Smith
发明人： Alan E. Smith
IPC分类号： A01K67027
CPC分类号： C12N15/8509 , A01K67/0278 , A01K2207/15 , A01K2217/00 , A01K2217/05 , A01K2227/10 , A01K2227/105 , A01K2267/01 , C07K14/705
摘要： Production of proteins not normally secreted through conventional pathways such as membrane proteins including, for example, CFTR associated with cystic fibrosis, is now made possible by collection of such protein from the milk of lactating transgenic animals.
摘要翻译：通常通过常规途径分泌的蛋白质（例如膜蛋白质，例如与囊性纤维化相关的CFTR）的产生现在可以通过从哺乳动物转基因动物的乳中收集这种蛋白质来实现。

9. 发明授权

US06448469B1 Production of membrane proteins in the milk of transgenic nonhuman mammals 失效
标题翻译：在转基因非人哺乳动物的乳中生产膜蛋白
公开(公告)号：US06448469B1
公开(公告)日：2002-09-10
申请号：US08088416
申请日：1993-07-07
申请人： Alan E. Smith
发明人： Alan E. Smith
IPC分类号： C12P2100
CPC分类号： C12N15/8509 , A01K67/0278 , A01K2207/15 , A01K2217/00 , A01K2217/05 , A01K2227/10 , A01K2227/105 , A01K2267/01 , C07K14/705
摘要： Production of proteins not normally secreted through conventional pathways such as membrane proteins including, for example, CFTR associated with cystic fibrosis, is now made possible by collection of such protein from the milk of lactating transgenic animals.
摘要翻译：通常通过常规途径分泌的蛋白质（例如包括例如与囊性纤维化相关的CFTR）的蛋白质的产生现在可以通过从哺乳动物转基因动物的乳中收集这样的蛋白质来实现。

10. 发明授权

US5877011A Chimeric adenoviral vectors 失效
标题翻译：嵌合腺病毒载体
公开(公告)号：US5877011A
公开(公告)日：1999-03-02
申请号：US752760
申请日：1996-11-20
申请人： Donna Armentano , Richard J. Gregory , Alan E. Smith
发明人： Donna Armentano , Richard J. Gregory , Alan E. Smith
IPC分类号： C12N15/09 , A61K48/00 , C07K14/075 , C12N15/861 , C12N15/86
CPC分类号： C12N15/86 , C07K14/005 , C12N2710/10322 , C12N2710/10343 , C12N2810/6018
摘要： A chimeric adenoviral vector is provided that comprises nucleotide sequence of a first adenovirus, wherein at least one gene of said first adenovirus encoding a protein that facilitates binding of said vector to a target mammalian cell, or internalization thereof within said cell, is replaced by the corresponding gene from a second adenovirus belonging to subgroup D, said vector further comprising a transgene operably linked to a eucaryotic promoter to allow for expression therefrom in a mammalian cell. Additionally, a method of delivering transgenes to target mammalian cells, particularly airway epithelial cells, is provided.
摘要翻译：提供了包含第一腺病毒的核苷酸序列的嵌合腺病毒载体，其中编码促进所述载体与靶哺乳动物细胞结合的蛋白质的所述第一腺病毒的至少一个基因或其在所述细胞内的内化被替换为来自属于亚组D的第二腺病毒的相应基因，所述载体还包含可操作地连接到真核启动子以在哺乳动物细胞中从其中表达的转基因。另外，提供了将转基因递送至靶哺乳动物细胞，特别是气道上皮细胞的方法。

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