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1. 发明公开

EP0833934A1 PACKAGING SYSTEMS FOR HUMAN RECOMBINANT ADENOVIRUS TO BE USED IN GENE THERAPY 失效
标题翻译：包装系统为保护人类，人腺病毒，用于基因治疗
公开(公告)号：EP0833934A1
公开(公告)日：1998-04-08
申请号：EP96917735.0
申请日：1996-06-14
申请人： Introgene B.V. , Rijksuniversiteit te Leiden
发明人： FALLAUX, Frits, Jacobus , HOEBEN, Robert, Cornelis , BOUT, Abraham , VALERIO, Domenico , VAN DER EB, Alex, Jan
IPC分类号： C12N , C12N15 , A61K35 , A61K48 , C12N5 , C12N7
CPC分类号： C12N7/00 , A61K48/00 , C12N15/86 , C12N2710/10321 , C12N2710/10343 , C12N2710/10351 , C12N2710/10352 , C12N2830/00 , C12N2830/15 , C12N2830/42 , C12N2830/60
摘要： The invention provides improved methods and products based on adenoviral materials which can advantageously be used in for instance gene therapy. In one aspect an adenoviral vector is provided which has no overlap with a suitable packaging cell line which is another aspect of invention. This combination excludes the possibility of homologous recombination, thereby excluding the possibility of the formation of replication competent adenovirus. In another aspect an adenovirus based helper construct which by its size is incapable of being encapsidated. This helper virus can be transferred into any suitable host cell making it a packaging cell. Further a number of useful mutations to adenoviral based materials and combinations of such mutations are disclosed, which all have in common the safety of the methods and the products, in particular avoiding the production of replication competent adenovirus and/or interference with the immune system. Further a method of intracellular amplification is provided.

2. 发明公开

EP1198559A1 MEANS AND METHODS FOR NUCLEIC ACID TRANSFER 审中-公开
标题翻译：手段与方法运输的NUCLEIC
公开(公告)号：EP1198559A1
公开(公告)日：2002-04-24
申请号：EP00925728.8
申请日：2000-04-25
申请人： Introgene B.V. , RIJKSUNIVERSITEIT LEIDEN
发明人： BOUT, Abraham , VALERIO, Domenico , SCHOUTEN, Govert, Johan , FALLAUX, Frits, Jacobus , HOEBEN, Robert, Cornelis , VAN DER EB, Alex, Jan
IPC分类号： C12N5/10 , C12N15/86
CPC分类号： C12N7/00 , C12N15/86 , C12N2710/10343 , C12N2710/10352
摘要： Cells capable of at least, in part, complementing adenovirus E2A function of an adenovirus defective in E2A function. Such cells include a nucleic acid encoding adenovirus E2A or a functional part, derivative and/or analogue thereof, integrated into the genome of the cell. Preferably, the cell has E2A nucleic acid derived from a temperature sensitive adenovirus such as adenovirus ts125. Methods for producing an adenovirus particle containing an adenovirus vector with a functional deletion of E2A. Such a method involves providing a cell as previously described with the functionally deleted adenovirus vector, culturing the cell, and harvesting the virus particle. The functional deletion can comprise a deletion of at least part of the nucleic acid encoding E2A. In such a method, the nucleic acid encoding adenovirus E2A in the genome of the cell preferably has no sequence overlap with the vector which leads to replication competent adenovirus and/or to the formation of an adenovirus vector comprising E2A function. In the method, the adenovirus vector preferably further comprises a functional deletion of E1-region encoding nucleic acid. Adenovirus vectors comprising a functional deletion of adenovirus E2A, preferably a deletion of at least part of the nucleic acid encoding E2A. Preparations of adenovirus vector containing adenovirus particles wherein the adenovirus vector comprises a functional deletion of E2A. Such an adenovirus vector preferably further includes a deletion of nucleic acid encoding E1-region proteins, and may be free of adenovirus vectors comprising E2A function. Methods for providing cells of an individual with a nucleic acid of interest, without risk of administering simultaneously a replication competent adenovirus vector, comprising administering the individual one of the previously described preparations.

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