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1. 发明公开

EP2607369A1 Modified nucleotides for polynucleotide sequencing 有权
标题翻译： Modifizierte Nucleotide zur Polynucleotidsequenzierung
公开(公告)号：EP2607369A1
公开(公告)日：2013-06-26
申请号：EP12180077.5
申请日：2003-08-22
申请人： Illumina Cambridge Limited
发明人： Milton, John , Wu, Xiaolin , Smith, Mark , Brennan, Joseph , Barnes, Colin , Liu, Xiaohai , Ruediger, Silke
IPC分类号： C07H19/06 , C07H19/10 , C07H19/16 , C07H19/20 , C07H21/00 , C12Q1/68
CPC分类号： C12Q1/6869 , C07H1/00 , C07H19/06 , C07H19/10 , C07H19/14 , C07H19/16 , C07H19/20 , C07H21/00 , C12Q2525/186 , C12Q2535/113 , Y02P20/55 , C12Q2525/197
摘要： The invention provides modified nucleotide or nucleoside molecule comprising a purine or pyrimidine base and a ribose or deoxyribose sugar moiety having a removable 3'-OH blocking group covalently attached thereto, such that the 3' carbon atom has attached a group of the structure

-O-Z

wherein Z is any of -C(R') 2 -O-R", -C(R') 2 -N(R") 2 ,-C(R') 2 -N(H)R", -C(R') 2 -S-R" and -C(R') 2 -F,
wherein each R" is or is part of a removable protecting group;
each R' is independently a hydrogen atom, an alkyl, substituted alkyl, arylalkyl, alkenyl, alkynyl, aryl, heteroaryl, heterocyclic, acyl, cyano, alkoxy, aryloxy, heteroaryloxy or amido group, or a detectable label attached through a linking group; or (R') 2 represents an alkylidene group of formula =C(R"') 2 wherein each R''' may be the same or different and is selected from the group comprising hydrogen and halogen atoms and alkyl groups; and
wherein said molecule may be reacted to yield an intermediate in which each R" is exchanged for H or, where Z is -C(R') 2 -F, the F is exchanged for OH, SH or NH 2 , preferably OH, which intermediate dissociates under aqueous conditions to afford a molecule with a free 3'OH;
with the proviso that where Z is -C(R') 2 -S-R", both R' groups are not H.
摘要翻译：本发明提供了包含嘌呤或嘧啶碱基和核糖或脱氧核糖糖部分的修饰的核苷酸或核苷分子，其具有共价连接到其上的可移除的3'-OH封闭基团，使得3'碳原子连接有一组结构 ƒ€ƒ€ƒ€ƒ€ƒ-OZ其中Z是-C（R'）2 -OR“，-C（R'）2 -N（R”）2，-C（ R'）2 -N（H）R“，-C（R'）2 -SR”和-C（R'）2 -F，其中每个R“为或可除去保护基的一部分;每个R' 氰基，烷氧基，芳氧基，杂芳氧基或酰胺基，或通过连接基团连接的可检测标记;或（R '）2表示式= C（R“'）2的亚烷基，其中每个R”可以相同或不同，并且选自氢和卤素原子和烷基; 并且其中所述分子可以反应以产生其中每个R“被交换为H的中间体，或者其中Z是-C（R'）2 -F，F被换成OH，SH或NH 2，优选OH，该中间体在水性条件下解离以得到具有游离3'OH的分子;条件是其中Z为-C（R'）2 -SR“，两个R'基团均不为H.

2. 发明公开

EP3438116A1 LABELLED NUCLEOTIDES 有权转让
公开(公告)号：EP3438116A1
公开(公告)日：2019-02-06
申请号：EP18195197.1
申请日：2003-08-22
申请人： Illumina Cambridge Limited
发明人： Milton, John , Ruediger, Silke , Liu, Xiaohai
IPC分类号： C07H19/06 , C07H19/10 , C07H19/16 , C07H19/20 , C07H21/00 , C12Q1/68 , G01N33/53
摘要： The invention provides a nucleotide or nucleoside having a base attached to a detectable label via a linker containing an azido group.

3. 发明公开

EP3795577A1 MODIFIED NUCLEOTIDES 无效
公开(公告)号：EP3795577A1
公开(公告)日：2021-03-24
申请号：EP20168283.8
申请日：2003-08-22
申请人： Illumina Cambridge Limited
发明人： Xiaolin, Wu , Smith, Mark , Milton, John , Brennan, Joseph , Barnes, Colin , Liu, Xiaohai , Ruediger, Silke
IPC分类号： C07H19/10 , C07H19/20 , C07H21/00 , C12Q1/68
摘要： The invention provides methods of determining the sequence of a target single-stranded polynucleotide, comprising monitoring the sequential incorporation of complementary nucleotides, wherein incorporation is controlled by incorporating a modified nucleotide molecule, the incorporation of said nucleotide molecule preventing introduction of subsequent nucleotide molecules into the growing complementary polynucleotide.

4. 发明授权

EP3147292B1 LABELLED NUCLEOTIDES 有权
公开(公告)号：EP3147292B1
公开(公告)日：2018-09-26
申请号：EP16193088.8
申请日：2003-08-22
申请人： Illumina Cambridge Limited
发明人： Milton, John , Ruediger, Silke , Liu, Xiaohai
IPC分类号： C07H19/06 , C07H19/10 , C07H19/16 , C07H19/20 , C07H21/00 , C12Q1/68 , G01N33/53
CPC分类号： C07H19/06 , C07H19/10 , C07H19/16 , C07H19/20 , C07H21/00 , C12Q1/6869 , C12Q2525/186 , C12Q2535/113
摘要： The invention provides a nucleoside or nucleotide having a base attached to a detectable label via a cleavable linker characterised in that the cleavable linker comprises an allyl or azido group.

5. 发明公开

EP3363809A1 MODIFIED NUCLEOTIDES FOR POLYNUCLEOTIDE SEQUENCING 有权转让
公开(公告)号：EP3363809A1
公开(公告)日：2018-08-22
申请号：EP18157128.2
申请日：2003-08-22
申请人： Illumina Cambridge Limited
发明人： Milton, John , Wu, Xiaolin , Smith, Mark , Brennan, Joseph , Barnes, Colin , Liu, Xiaohai , Ruediger, Silke
IPC分类号： C07H19/06 , C07H19/10 , C07H19/16 , C07H19/20 , C07H21/00 , C12Q1/68 , G01N33/53
CPC分类号： C12Q1/6869 , C07H1/00 , C07H19/06 , C07H19/10 , C07H19/14 , C07H19/16 , C07H19/20 , C07H21/00 , C12Q2525/186 , C12Q2535/113 , Y02P20/55 , C12Q2525/197
摘要： The invention provides modified nucleotide triphosphate molecules comprising a purine or pyrimidine base and a deoxyribose sugar moiety having a 3'-azidomethyl group.

6. 发明公开

EP3147292A1 LABELLED NUCLEOTIDES 有权
标题翻译： MARKIERTE NUKLEOTIDE
公开(公告)号：EP3147292A1
公开(公告)日：2017-03-29
申请号：EP16193088.8
申请日：2003-08-22
申请人： Illumina Cambridge Limited
发明人： Milton, John , Ruediger, Silke , Liu, Xiaohai
IPC分类号： C07H19/06 , C07H19/10 , C07H19/16 , C07H19/20 , C07H21/00 , C12Q1/68 , G01N33/53
CPC分类号： C07H19/06 , C07H19/10 , C07H19/16 , C07H19/20 , C07H21/00 , C12Q1/6869 , C12Q2525/186 , C12Q2535/113
摘要： The invention provides a nucleotide or nucleoside having a base attached to a detectable label via a cleavable linker, characterised in that the cleavable linker contains a moiety selected from the group comprising:

(wherein X is selected from the group comprising O, S, NH and NQ wherein Q is a C 1-10 substituted or unsubstituted alkyl group, Y is selected from the group comprising O, S, NH and N(allyl), T is hydrogen or a C 1-10 substituted or unsubstituted alkyl group and * indicates where the moiety is connected to the remainder of the nucleotide or nucleoside).
摘要翻译：本发明提供了具有通过可切割接头连接到可检测标记的碱基的核苷酸或核苷，其特征在于可切割连接体含有选自以下的部分：（其中X选自O，S，NH和 NQ其中Q是C 1-10取代或未取代的烷基，Y选自O，S，NH和N（烯丙基），T是氢或C 1-10取代或未取代的烷基，*表示其中部分连接到核苷酸或核苷的剩余部分）。

7. 发明授权

EP2119722B1 Labelled nucleotides 有权
标题翻译：标记核苷酸
公开(公告)号：EP2119722B1
公开(公告)日：2016-10-26
申请号：EP09005942.9
申请日：2003-08-22
申请人： Illumina Cambridge Limited
发明人： Milton, John , Ruediger, Silke , Liu, Xiaohai
IPC分类号： C07H19/06 , C07H19/10 , C07H19/16 , C07H19/20 , C07H21/00 , C12Q1/68 , G01N33/53
CPC分类号： C07H19/06 , C07H19/10 , C07H19/16 , C07H19/20 , C07H21/00 , C12Q1/6869 , C12Q2525/186 , C12Q2535/113

8. 发明授权

EP2607369B1 Modified nucleotides for polynucleotide sequencing 有权
标题翻译：修饰的核苷酸为多核苷酸测序
公开(公告)号：EP2607369B1
公开(公告)日：2015-09-23
申请号：EP12180077.5
申请日：2003-08-22
申请人： Illumina Cambridge Limited
发明人： Milton, John , Wu, Xiaolin , Smith, Mark , Brennan, Joseph , Barnes, Colin , Liu, Xiaohai , Ruediger, Silke
IPC分类号： C07H19/06 , C07H19/10 , C07H19/16 , C07H19/20 , C07H21/00 , C12Q1/68
CPC分类号： C12Q1/6869 , C07H1/00 , C07H19/06 , C07H19/10 , C07H19/14 , C07H19/16 , C07H19/20 , C07H21/00 , C12Q2525/186 , C12Q2535/113 , Y02P20/55 , C12Q2525/197
摘要： The invention provides modified nucleotide or nucleoside molecule comprising a purine or pyrimidine base and a ribose or deoxyribose sugar moiety having a removable 3'-OH blocking group covalently attached thereto, such that the 3' carbon atom has attached a group of the structure €ƒ€ƒ€ƒ€ƒ€ƒ€ƒ€ƒ€ƒ-O-Z wherein Z is any of -C(R') 2 -O-R", -C(R') 2 -N(R") 2 ,-C(R') 2 -N(H)R", -C(R') 2 -S-R" and -C(R') 2 -F, wherein each R" is or is part of a removable protecting group; each R' is independently a hydrogen atom, an alkyl, substituted alkyl, arylalkyl, alkenyl, alkynyl, aryl, heteroaryl, heterocyclic, acyl, cyano, alkoxy, aryloxy, heteroaryloxy or amido group, or a detectable label attached through a linking group; or (R') 2 represents an alkylidene group of formula =C(R"') 2 wherein each R''' may be the same or different and is selected from the group comprising hydrogen and halogen atoms and alkyl groups; and wherein said molecule may be reacted to yield an intermediate in which each R" is exchanged for H or, where Z is -C(R') 2 -F, the F is exchanged for OH, SH or NH 2 , preferably OH, which intermediate dissociates under aqueous conditions to afford a molecule with a free 3'OH; with the proviso that where Z is -C(R') 2 -S-R", both R' groups are not H.

9. 发明授权

EP3363809B1 MODIFIED NUCLEOTIDES FOR POLYNUCLEOTIDE SEQUENCING 有权转让
公开(公告)号：EP3363809B1
公开(公告)日：2020-04-08
申请号：EP18157128.2
申请日：2003-08-22
申请人： Illumina Cambridge Limited
发明人： Milton, John , Wu, Xiaolin , Smith, Mark , Brennan, Joseph , Barnes, Colin , Liu, Xiaohai , Ruediger, Silke
IPC分类号： C07H19/06 , C07H19/10 , C07H19/16 , C07H19/20 , C07H21/00 , C12Q1/68 , G01N33/53

10. 发明公开

EP3587433A1 MODIFIED NUCLEOTIDES 有权
公开(公告)号：EP3587433A1
公开(公告)日：2020-01-01
申请号：EP19164666.0
申请日：2003-08-22
申请人： Illumina Cambridge Limited
发明人： Wu, Xiaolin , Smith, Mark , Milton, John , Brennan, Joseph , Barnes, Colin , Liu, Xiaohai , Ruediger, Silke
IPC分类号： C07H19/10 , C07H19/20 , C07H21/00 , C12Q1/68
摘要： The invention provides a kit comprising four modified nucleotide triphosphate molecules, each comprising a purine or pyrimidine base and a deoxyribose sugar moiety wherein the 3' carbon atom of the sugar moiety has attached a group of the structure

-O-Z

wherein Z is of the formula -CH 2 N 3 .

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