专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明公开

EP2076609A1 COMPOSITIONS AND METHODS FOR REPRESENTATIONAL SELECTION OF NUCLEIC ACIDS FRO COMPLEX MIXTURES USING HYBRIDIZATION 审中-公开
标题翻译：组合物和方法从复杂的混合物杂交NUCLEIC代表选择
公开(公告)号：EP2076609A1
公开(公告)日：2009-07-08
申请号：EP07837278.6
申请日：2007-08-24
申请人： Illumina Inc.
发明人： STUELPNAGEL, John R. , BARKER, David L. , VELARDE, Jorge Jr. , BARNARD, Steven M. , GRAIGE, Michael
IPC分类号： C12Q1/68
CPC分类号： C12N15/1072 , C12Q1/6837 , C12Q2565/515
摘要： The invention provides a method of selecting a representational sample of nucleic acid sequences from a complex mixture. The method includes: (a) contacting a complex mixture of nucleic acids under conditions sufficient for hybridization with a population of capture probes complementary to one or more nucleic acids comprising a predetermined portion of the sequence collectively present in the complex mixture to form hybridization complexes of the one or more nucleic acids with the population of probes, the population of capture probes being attached to a solid support, and (b) removing unhybridized nucleic acids to select a representational sample of nucleic acids having a complexity of less than 10% but more than 0.001% of the complex mixture, wherein the representational sample comprises a nucleic acid copy having a proportion of each sequence in the copy relative to all other sequences in the copy substantially the same as the proportions of the sequences in the predetermined portion of one or more nucleic acids within the complex mixture. A method of selecting a representational sample of genomic sequences from a complete genome also is provided. The invention further provides a nucleic acid population that includes a representational sample having a complexity of less than 10% but more than 0.001% of a complex mixture, the representational sample comprising a nucleic acid copy having a proportion of each sequence in the copy relative to all other sequences in the copy substantially the same as the proportions of sequences in a predetermined portion of a sequence collectively present in one or more nucleic acids within the complex mixture.

2. 发明申请

WO2017172798A1 MULTI-PLANE MICROARRAYS 审中-公开
标题翻译：多平面微型阵列
公开(公告)号：WO2017172798A1
公开(公告)日：2017-10-05
申请号：PCT/US2017/024578
申请日：2017-03-28
申请人： ILLUMINA, INC. , BOWEN, M. Shane
发明人： BOWEN, M. Shane , GRAIGE, Michael , HONG, Stanley S. , MOON, John A. , SIU, Merek
IPC分类号： B01L3/00 , G01N33/543 , G01N33/53 , C12Q1/68
摘要： An array including a solid support having a plurality of contours along its exterior surface. A first subset of contours is positioned along the exterior surface of the solid support to form a first pattern of features and a second subset of contours is positioned along the exterior surface to form a second pattern of features. The contours of the first subset are juxtaposed with the second subset along the exterior surface, whereby the first and second patterns form an interleaved pattern. The features of the first pattern occur at a first elevation z1 and the features of the second pattern occur at a second elevation z2. The features of the first pattern are configured to attach analytes at a different elevation relative to analytes attached to the features of the second pattern.
摘要翻译：包括具有沿其外表面的多个轮廓的固体支撑件的阵列。轮廓的第一子集沿固体支撑件的外表面定位以形成特征的第一图案，轮廓的第二子集沿着外表面定位以形成特征的第二图案。第一子集的轮廓沿着外表面与第二子集并置，由此第一和第二图案形成交错图案。第一图案的特征出现在第一高程z1处，并且第二图案的特征出现在第二高度z2处。第一种图案的特征被配置为以与附着于第二图案的特征的分析物不同的高度附着分析物。

3. 发明申请

WO2008045158A1 COMPOSITIONS AND METHODS FOR REPRESENTATIONAL SELECTION OF NUCLEIC ACIDS FRO COMPLEX MIXTURES USING HYBRIDIZATION 审中-公开
标题翻译：使用杂化代表性选择核酸FRO复合物的组合物和方法
公开(公告)号：WO2008045158A1
公开(公告)日：2008-04-17
申请号：PCT/US2007/018687
申请日：2007-08-24
申请人： ILLUMINA, INC. , STUELPNAGEL, John, R. , BARKER, David, L. , VELARDE, Jorge, Jr. , BARNARD, Steven, M. , GRAIGE, Michael
发明人： STUELPNAGEL, John, R. , BARKER, David, L. , VELARDE, Jorge, Jr. , BARNARD, Steven, M. , GRAIGE, Michael
IPC分类号： C12Q1/68
CPC分类号： C12N15/1072 , C12Q1/6837 , C12Q2565/515
摘要： The invention provides a method of selecting a representational sample of nucleic acid sequences from a complex mixture. The method includes: (a) contacting a complex mixture of nucleic acids under conditions sufficient for hybridization with a population of capture probes complementary to one or more nucleic acids comprising a predetermined portion of the sequence collectively present in the complex mixture to form hybridization complexes of the one or more nucleic acids with the population of probes, the population of capture probes being attached to a solid support, and (b) removing unhybridized nucleic acids to select a representational sample of nucleic acids having a complexity of less than 10% but more than 0.001% of the complex mixture, wherein the representational sample comprises a nucleic acid copy having a proportion of each sequence in the copy relative to all other sequences in the copy substantially the same as the proportions of the sequences in the predetermined portion of one or more nucleic acids within the complex mixture. A method of selecting a representational sample of genomic sequences from a complete genome also is provided. The invention further provides a nucleic acid population that includes a representational sample having a complexity of less than 10% but more than 0.001% of a complex mixture, the representational sample comprising a nucleic acid copy having a proportion of each sequence in the copy relative to all other sequences in the copy substantially the same as the proportions of sequences in a predetermined portion of a sequence collectively present in one or more nucleic acids within the complex mixture.
摘要翻译：本发明提供了从复杂混合物中选择核酸序列的表示样品的方法。该方法包括：（a）在足以与包含与复合物混合物中共同存在的序列的预定部分的一个或多个核酸互补的捕获探针的杂交群的条件下使核酸的复杂混合物接触，以形成杂合复合物具有探针群的一个或多个核酸，捕获探针的群体连接到固体支持物上，和（b）除去未杂交的核酸以选择复杂度小于10％但更多的核酸的代表性样品超过0.001％的复合混合物，其中代表性样品包含核酸拷贝，其拷贝中的每个序列的比例相对于拷贝中的所有其它序列基本上与在一个或更多个预定部分中的序列的比例相同复杂混合物中更多的核酸。还提供了从完整基因组中选择基因组序列的表示样品的方法。本发明还提供了一种核酸群体，其包括具有小于复合物混合物的10％但大于0.001％的复杂性的代表性样品，所述代表性样品包含核酸拷贝，所述核酸拷贝具有拷贝中每个序列的比例相对于复制中的所有其它序列基本上与复合物混合物中一个或多个核酸中共存的序列的预定部分中的序列比例相同。

4. 发明公开

EP3377226A1 MULTI-PLANE MICROARRAYS 有权
公开(公告)号：EP3377226A1
公开(公告)日：2018-09-26
申请号：EP17776477.6
申请日：2017-03-28
申请人： Illumina, Inc.
发明人： BOWEN, M. Shane , GRAIGE, Michael , HONG, Stanley S. , MOON, John A. , SIU, Merek
IPC分类号： B01L3/00 , B01J19/00 , C12Q1/6837 , C12Q1/6844 , C12Q1/6874
CPC分类号： B01L3/5085 , B01J19/0046 , B01J2219/00317 , B01J2219/00509 , B01J2219/00621 , B01J2219/00662 , B01J2219/00668 , B01J2219/00722 , B01L2300/0636 , B01L2300/0819 , B01L2300/0848 , B01L2300/0851 , C12Q1/6837 , C12Q1/6844 , C12Q1/6874 , C12Q2565/537
摘要： An array including a solid support having a plurality of contours along its exterior surface. A first subset of contours is positioned along the exterior surface of the solid support to form a first pattern of features and a second subset of contours is positioned along the exterior surface to form a second pattern of features. The contours of the first subset are juxtaposed with the second subset along the exterior surface, whereby the first and second patterns form an interleaved pattern. The features of the first pattern occur at a first elevation z1 and the features of the second pattern occur at a second elevation z2. The features of the first pattern are configured to attach analytes at a different elevation relative to analytes attached to the features of the second pattern.

5. 发明公开

EP3831484A1 MULTI-PLANE MICROARRAYS 有权
公开(公告)号：EP3831484A1
公开(公告)日：2021-06-09
申请号：EP20217230.0
申请日：2017-03-28
申请人： Illumina, Inc.
发明人： BOWEN, Shane , GRAIGE, Michael , HONG, Stanley , MOON, John , SIU, Merek
IPC分类号： B01L3/00 , B01J19/00 , C12Q1/6837 , C12Q1/6844 , C12Q1/6874
摘要： An array including a solid support having a plurality of contours along its exterior surface. A first subset of contours is positioned along the exterior surface of the solid support to form a first pattern of features and a second subset of contours is positioned along the exterior surface to form a second pattern of features. The contours of the first subset are juxtaposed with the second subset along the exterior surface, whereby the first and second patterns form an interleaved pattern. The features of the first pattern occur at a first elevation z 1 and the features of the second pattern occur at a second elevation z 2 . The features of the first pattern are configured to attach analytes at a different elevation relative to analytes attached to the features of the second pattern.

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式