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    • 2. 发明授权
    • Method for whole surrounding surface display of target proteins using bacterial exosporium
    • 使用细菌外植体的靶蛋白的整个周围表面显示的方法
    • US08030064B2
    • 2011-10-04
    • US10572224
    • 2003-12-30
    • Sang Yup LeeTae Jung ParkJong Pil ParkSeok Jae LeeJae-Gu PanHeung-Chae JungSoo-Keun Choi
    • Sang Yup LeeTae Jung ParkJong Pil ParkSeok Jae LeeJae-Gu PanHeung-Chae JungSoo-Keun Choi
    • C12N15/00C12N1/21
    • C40B40/02C12N15/1037
    • The present invention relates to a method for expressing a target protein on an exosporium forming the outermost surface of bacterial spores. More particularly, the present invention relates to a method for expressing a target protein on the surface of cells and spores using an exosporium as a matrix for surface expression, and methods for the production of a protein array, the production of antibodies, the separation of a certain substance from a mixture, bioconversion, and the improvement of a target protein, which are characterized by using the cells or spores having the target protein that was expressed on the surface by the above expression method. The method for expressing the target protein on the surface of the spore outer membrane of the gene carriers according to the present invention has effects in that a variety of the target proteins can be expressed and the level of surface expression of the target protein is increased compared to the existing technology, and also the structural stability of the gene carriers having the target protein expressed on their surface, the viability of the host, and the rapidity of the screening method, are greatly increased.
    • 本发明涉及在形成细菌孢子的最外表面的外孢上表达靶蛋白的方法。 更具体地说,本发明涉及使用外鎓作为表面表达基质在细胞和孢子表面上表达靶蛋白的方法,以及用于产生蛋白质阵列,产生抗体的方法,分离 特定于使用具有通过上述表达方法在表面表达的靶蛋白的细胞或孢子的来自混合物的某种物质,生物转化和靶蛋白的改良。 根据本发明的用于在基因载体的孢子外膜的表面上表达靶蛋白的方法具有以下效果:可以表达多种靶蛋白,并且与目标蛋白的表面表达水平相比提高了靶蛋白的表达表达水平 现有技术,以及表面上具有靶蛋白的基因载体的结构稳定性,宿主的活力以及筛选方法的快速性都大大提高。