专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明申请

WO2007050100A2 IMMOBILIZED ENZYMES AND PROCESSES FOR PREPARING AND USING SAME 审中-公开
标题翻译：固定化酶及其制备和使用过程
公开(公告)号：WO2007050100A2
公开(公告)日：2007-05-03
申请号：PCT/US2005/042657
申请日：2005-11-23
申请人： INDUSTRIAL SCIENCE & TECHNOLOGY NETWORK, INC. , DAVID, Allan , YANG, Arthur
发明人： DAVID, Allan , YANG, Arthur
IPC分类号： C12N11/14
CPC分类号： C12N11/14 , C12N9/54 , C12P21/06
摘要： A highly loaded immobilized enzyme conjugate is prepared from a nanopore silica gel support. The enzyme may be immobilized in an amount, based on the silica gel support, of at least about 500 mg enzyme per gram of silica gel. The immobilized enzyme conjugate may be prepared by a method which includes the following steps: (a) surface modifying silicic acid by reacting the silicic acid with a basic aminoalkylsilane in solution in an alcoholic medium, wherein the amount of the basic aminoalkylsilane is sufficient to raise the pH of the solution to induce gelation and form a chemically surface-modified silica gel; (b) activating the chemically surface-modified silica gel by reaction with a cross-linking agent; and (c) contacting the activated chemically surface-modified silica gel with an enzyme. Immobilized thermolysin has been used to prepare a low molecular weight protamine with high efficiency.
摘要翻译：从纳米孔硅胶载体制备高负载的固定化酶缀合物。基于硅胶载体，酶可以固定在至少约500mg酶/克硅胶的量。可通过包括以下步骤的方法制备固定化酶缀合物：（a）通过硅酸与碱性氨基烷基硅烷在醇类介质中的溶液中反应来表面改性硅酸，其中碱性氨基烷基硅烷的量足以提高该溶液的pH诱导凝胶化并形成化学表面改性的硅胶; （b）通过与交联剂反应活化化学表面改性的硅胶; 和（c）使活化的化学表面改性的硅胶与酶接触。固定的嗜热菌蛋白酶已被用于高效率地制备低分子量鱼精蛋白。

IPRDB

热门服务

关于我们

友情链接

联系方式