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1. 发明申请

WO2013136280A1 PROCESS FOR THE RECOVERY OF L-FUCOSE FROM EXOPOLYSACCHARIDES 审中-公开
标题翻译：从异丙肾上腺素恢复L-糖蛋白的方法
公开(公告)号：WO2013136280A1
公开(公告)日：2013-09-19
申请号：PCT/IB2013/051994
申请日：2013-03-13
申请人： INALCO S.A.S. DI GIOVANNI CIPOLLETTI & C.
发明人： VAGNOLI, Luana , GIACOMELLI, Silvia , BIAGIOLINI, Silvia , CIPOLLETTI, Giovanni , GORI, Alessandro
IPC分类号： C07H1/08 , C13K13/00
CPC分类号： C07H1/08 , C07H3/02 , C13K13/00
摘要： The present invention relates to the isolation, on an industrial scale, of L-Fucose (or 6-Deoxygalactose) from exopolysaccharides obtained by fermentative process. The purification step is a crucial point of the L-Fucose recovery process: the polysaccharide is recovered by ultrafiltration in that it quantitatively remains in the retentate together with only the cells of the microorganism while all the culture medium residues, the fermentation by-products and the salts are eliminated in the permeate in an almost quantitative manner. This step excellently resolves the problem of the purification of the Fucose with a view to obtaining a high-purity crystal; it indeed allows separation of the sugars that make up the polysaccharide from all those by-products having a low molecular weight that cannot be eliminated in the subsequent steps and which, being present in considerable amounts, constitute a great problem for the direct crystallization of the product of the product.
摘要翻译：本发明涉及在工业规模上从通过发酵方法获得的外多糖分离L-岩藻糖（或6-脱氧半乳糖）。纯化步骤是L-岩藻糖回收过程的关键点：多糖通过超滤回收，因为它只与微生物的细胞一起定量保留在滞留物中，而所有的培养基残留物，发酵副产物和在几乎定量的方式在渗透物中消除盐。为了获得高纯度晶体，该步骤极好地解决了岩藻糖的纯化问题。确实允许从所有那些在随后的步骤中不能被消除的低分子量副产物的多糖分离出来，并且以相当的量存在，构成了直接结晶的重要问题产品的产品。

2. 发明申请

WO2014006606A1 ENZYMES IMMOBILISED ON STYRENE-DIVINYL BENZENE POLYMER MATRICES AND THE USE THEREOF IN INDUSTRIAL PRODUCTIONS 审中-公开
标题翻译：固定在苯乙烯 - 苯乙烯聚合物基体上的酶及其在工业生产中的应用
公开(公告)号：WO2014006606A1
公开(公告)日：2014-01-09
申请号：PCT/IB2013/055572
申请日：2013-07-08
申请人： INALCO S.A.S. DI GIOVANNI CIPOLLETTI & C.
发明人： CHINI, Jacopo , FEBBRUARI, Barbara , MATULLI, Marina , SPAGNESI, Sonia , VAGNOLI, Luana , GIACOMELLI, Silvia , TAMERLANI, Giancarlo , CIPOLLETTI, Giovanni
IPC分类号： C12N11/02 , C12N11/06 , C12N11/08
CPC分类号： C12N11/06 , C12N9/2468 , C12N11/08 , C12P19/02 , C12P19/14
摘要： The present invention discloses a process for immobilising enzymes, at industrial scale, on commercial styrene-divinyl benzene polymer matrix resins which are non-functionalised or functionalised with tertiary amine groups, by forming a stable bond between the matrix, which is activated with a di-aldehyde compound (such as glutaraldehyde) and the enzyme. The enzymes immobilised according to the invention are particularly useful for the industrial production of galactose and tagatose.
摘要翻译：本发明公开了一种将工业规模的酶固定在商业苯乙烯 - 二乙烯基苯聚合物基质树脂上的方法，该聚合物基质树脂是用叔胺基团官能化或官能化的，通过在基体之间形成稳定的键， - 醛化合物（如戊二醛）和酶。根据本发明固定的酶特别可用于半乳糖和塔格糖的工业生产。

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