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1. 发明申请

WO2004009796A3 SINGLE PROMOTER SYSTEM FOR MAKING SIRNA EXPRESSION CASSETTES AND EXPRESSION LIBRARIES USING A POLYMERASE PRIMER HAIRPIN LINKER 审中-公开
标题翻译：使用聚合酶原乳头连接器制造SIRNA表达盒和表达图案的单个促进器系统
公开(公告)号：WO2004009796A3
公开(公告)日：2005-03-17
申请号：PCT/US0323239
申请日：2003-07-23
申请人： IMMUSOL INC , LI HENRY , CHATTERTON JON E , KE NING , RHOADES KRISTINA L , WONG-STAAL FLOSSIE
发明人： LI HENRY , CHATTERTON JON E , KE NING , RHOADES KRISTINA L , WONG-STAAL FLOSSIE
IPC分类号： C12N15/09 , C12N15/11 , C12N15/66 , C12N15/63
CPC分类号： C12N15/111 , C12N15/66 , C12N2310/111 , C12N2310/14 , C12N2310/53 , C12N2330/31 , C12N2799/027
摘要： The present invention relates to methods and compositions for the elucidation of gene function and the identification of novel genes. Specifically, the present invention relates to methods and compositions for improved functional genomic screening, functional inactivation of specific essential or non-essential genes, and identification of genes that are modulated in response to specific stimuli or encode recognizable phenotypic traits. In particular, the compositions of the present invention include, but are not limited to, expression cassettes comprising a novel polymerase primer hairpin linker that allows rapid construction of a single transcriptional unit encoding both strands of a hairpin siRNA, regardless of sequence. In addition, the present invention includes libraries comprising the expression cassettes of the invention, including vectors for transforming cells, such as replication-deficient retroviral vectors. The invention also includes methods for the production and screening of siRNA libraries, as well as therapeutic uses for the siRNAs expressed in accordance with the invention.
摘要翻译：本发明涉及用于阐明基因功能和鉴定新基因的方法和组合物。具体而言，本发明涉及用于改进功能基因组筛选，特异性必需或非必需基因的功能失活以及根据特定刺激或编码可识别的表型性状调节的基因的鉴定的方法和组合物。特别地，本发明的组合物包括但不限于包含新型聚合酶底漆发夹接头的表达盒，其允许快速构建编码发夹siRNA两条链的单一转录单位，而不管序列如何。此外，本发明包括包含本发明的表达盒的文库，包括用于转化细胞的载体，例如复制缺陷型逆转录病毒载体。本发明还包括用于生产和筛选siRNA文库的方法以及根据本发明表达的siRNA的治疗用途。

2. 发明申请

WO2004072261A3 SIRNA LIBRARIES OPTIMIZED FOR PREDETERMINED PROTEIN FAMILIES 审中-公开
标题翻译：针对预测蛋白家族优化的SIRNA图书馆
公开(公告)号：WO2004072261A3
公开(公告)日：2005-06-09
申请号：PCT/US2004003949
申请日：2004-02-10
申请人： IMMUSOL INC , LI HENRY , CHATTERTON JON E , FAN WUFANG , KE NING , WONG-STAAL FLOSSIE
发明人： LI HENRY , CHATTERTON JON E , FAN WUFANG , KE NING , WONG-STAAL FLOSSIE
IPC分类号： C12N15/11 , C12N15/113 , C07H21/00
CPC分类号： C12N15/111 , C12N15/1137 , C12N15/1138 , C12N2310/111 , C12N2310/14 , C12N2310/53 , C12N2330/31 , C12Y207/10001 , C12Y207/10002
摘要： siRNA Libraries Optimized for Predetermined Protein Families ABSTRACT Libraries for generating small inhibitory RNA (siRNA) are provided where the members of the library are optimized to inhibit the expression of genes that encode a predetermined family of proteins. The members of the library target at least mRNA encoding all members of the family of proteins. Methods for generating siRNA libraries of the present invention are also provided.
摘要翻译：针对预定蛋白家族优化的siRNA文库摘要提供用于产生小抑制性RNA（siRNA）的文库，其中文库成员被优化以抑制编码预定蛋白质家族的基因的表达。图书馆的成员至少靶向编码蛋白质家族成员的mRNA。还提供了用于产生本发明的siRNA文库的方法。

3. 发明申请

WO2004009794A2 NOVEL SIRNA GENE LIBRARIES AND METHODS FOR THEIR PRODUCTION AND USE 审中-公开
标题翻译：新的SIRNA基因库及其生产和使用方法
公开(公告)号：WO2004009794A2
公开(公告)日：2004-01-29
申请号：PCT/US0323157
申请日：2003-07-23
申请人： IMMUSOL INC , LI HENRY , CHATTERTON JON E , KE NING , WONG-STAAL FLOSSIE
发明人： LI HENRY , CHATTERTON JON E , KE NING , WONG-STAAL FLOSSIE
IPC分类号： C12N15/09 , C12N15/10 , C12N15/11 , C12N
CPC分类号： C12N15/111 , C12N15/1075 , C12N2310/111 , C12N2310/14 , C12N2310/53 , C12N2330/31
摘要： The present invention relates to methods and compositions for the elucidation of gene function and the identification of novel genes. Specifically, the present invention relates to methods and compositions for improved functional genomic screening, functional inactivation of specific essential or non-essential genes, and identification of genes that are modulated in response to specific stimuli or encode recognizable phenotypic traits. In particular, the compositions of the present invention include, but are not limited to, expression cassettes comprising a novel dual promoter transcription system, that utilizes modified promoters, preferably containing complementary termination sequences, positioned across a coding sequence and in opposite orientation to each other. In addition, the present invention includes libraries comprising the expression cassettes of the invention, including vectors for transforming cells, such as replication-deficient retroviral vectors. The invention also includes methods for the production and screening of dsRNA/siRNA libraries, as well as therapeutic uses for the siRNAs expressed in accordance with the invention.
摘要翻译：本发明涉及阐明基因功能和鉴定新基因的方法和组合物。具体而言，本发明涉及用于改进功能基因组筛选，特异性必需或非必需基因的功能失活以及根据特定刺激或编码可识别的表型性状调节的基因的鉴定的方法和组合物。特别地，本发明的组合物包括但不限于包含新颖的双重启动子转录系统的表达盒，其利用修饰的启动子，优选含有互补终止序列，跨越编码序列并且彼此相反取向。此外，本发明包括包含本发明的表达盒的文库，包括用于转化细胞的载体，例如复制缺陷型逆转录病毒载体。本发明还包括用于生产和筛选dsRNA / siRNA文库的方法以及根据本发明表达的siRNA的治疗用途。

4. 发明申请

WO2006078880A3 INDUCIBLE SIRNA EXPRESSION CASSETTE AND METHOD OF USE 审中-公开
标题翻译：可诱导SIRNA表达载体及其使用方法
公开(公告)号：WO2006078880A3
公开(公告)日：2006-11-09
申请号：PCT/US2006001997
申请日：2006-01-18
申请人： IMMUSOL INC , ZHOU DEMIN , CHATTERTON JON E , KE NING , ZHANG JING , BLIESATH JOSHUA , LI HENRY , WONG-STAAL FLOSSIE
发明人： ZHOU DEMIN , CHATTERTON JON E , KE NING , ZHANG JING , BLIESATH JOSHUA , LI HENRY , WONG-STAAL FLOSSIE
IPC分类号： C12N15/00 , A01K67/00 , C07H21/04 , C12N5/00 , C12N15/63 , C12N15/82
CPC分类号： C12N15/86 , A01K2267/0331 , C12N15/111 , C12N15/113 , C12N2310/111 , C12N2310/14 , C12N2320/10 , C12N2320/30 , C12N2320/50 , C12N2330/31 , C12N2740/15043 , C12N2830/006 , C12N2840/203
摘要： An inducible siRNA expression polynucleotide and methods for its use are provided. The expression polynucleotide comprises a bicistronic expression cassette that encodes a repressor and a detectable marker, wherein the repressor controls expression of siRNA expression in the absence of an inducer.
摘要翻译：提供可诱导的siRNA表达多核苷酸及其使用方法。表达多核苷酸包含编码阻抑物和可检测标记的双顺反子表达盒，其中所述阻遏物在不存在诱导物的情况下控制siRNA表达的表达。

5. 发明申请

WO2005017112A2 METHODS OF INHIBITING CANCER GROWTH BY BINDING TO NUCLEAR RECEPTORS 审中-公开
标题翻译：通过与核受体结合抑制癌症生长的方法
公开(公告)号：WO2005017112A2
公开(公告)日：2005-02-24
申请号：PCT/US2004023821
申请日：2004-07-23
申请人： IMMUSOL INC , LI HENRY , KE NING , GRIFMAN MIRTA , CLAASSEN GISELA , HU XIUYUAN , DEFIFE KRISTIN , HABITA CELLIA , FAN WUFANG , RHOADES KRISTINA , TAN PHILIP , WONG-STAAL FLOSSIE
发明人： LI HENRY , KE NING , GRIFMAN MIRTA , CLAASSEN GISELA , HU XIUYUAN , DEFIFE KRISTIN , HABITA CELLIA , FAN WUFANG , RHOADES KRISTINA , TAN PHILIP , WONG-STAAL FLOSSIE
IPC分类号： C12N20060101 , C12N15/113 , G01N33/50 , C12N
CPC分类号： G01N33/5011 , C12N15/1138 , C12N2310/121 , C12N2310/14 , G01N2510/00
摘要： The present invention provides methods for identifying agents useful for inhibiting cancer cells by binding to various nuclear receptor proteins, or to the genes or RNA encoding such proteins.
摘要翻译：本发明提供了通过结合各种核受体蛋白质或编码这些蛋白质的基因或RNA鉴定可用于抑制癌细胞的药剂的方法。

6. 发明申请

WO2006020557A3 METHODS OF USING OR IDENTIFYING AGENTS THAT INHIBIT CANCER GROWTH 审中-公开
标题翻译：使用或识别抑制癌症生长的药物的方法
公开(公告)号：WO2006020557A3
公开(公告)日：2007-08-16
申请号：PCT/US2005028100
申请日：2005-08-09
申请人： IMMUSOL INC , QI-XIAN LI HENRY , KE NING , GRIFMAN MIRTA , FAN WUFANG , DEHUA YU , WONG-STAAL FLOSSIE
发明人： QI-XIAN LI HENRY , KE NING , GRIFMAN MIRTA , FAN WUFANG , DEHUA YU , WONG-STAAL FLOSSIE
IPC分类号： A61K31/70
CPC分类号： C07K16/2863 , C07K14/705 , C07K2317/34 , C07K2317/73 , C12N15/1138 , C12N2310/111 , C12N2310/14
摘要： The present invention is directed in general to methods of inhibiting the growth of cancer cells or tumors. The present invention is also directed to identifying agents useful for the treatment of cancer. These methods generally involve targeting two genes or their encoded proteins, GPR56 or DNER.
摘要翻译：本发明总体上涉及抑制癌细胞或肿瘤生长的方法。本发明还涉及鉴定可用于治疗癌症的药剂。这些方法通常涉及靶向两种基因或其编码的蛋白质GPR56或DNER。

7. 发明申请

WO2005023186A2 METHODS OF IDENTIFYING AGENTS THAT INHIBIT THE GROWTH OF CANCER CELLS 审中-公开
标题翻译：鉴定抑制癌细胞生长的药物的方法
公开(公告)号：WO2005023186A2
公开(公告)日：2005-03-17
申请号：PCT/US2004027672
申请日：2004-08-24
申请人： IMMUSOL INC , HU XIUYUAN , LI HENRY , KE NING , GRIFMAN MIRTA , ROGERS CHERYL , DEFIFE KRISTIN , HABITA CELLIA , FAN WUFANG , RHOADES KRISTINA , TAN PHILIP , TRITZ RICHARD , WONG-STAAL FLOSSIE
发明人： HU XIUYUAN , LI HENRY , KE NING , GRIFMAN MIRTA , ROGERS CHERYL , DEFIFE KRISTIN , HABITA CELLIA , FAN WUFANG , RHOADES KRISTINA , TAN PHILIP , TRITZ RICHARD , WONG-STAAL FLOSSIE
IPC分类号： A61K20060101 , C12N15/113 , G01N33/50 , A61K
CPC分类号： G01N33/5011 , C12N15/113 , C12N15/1137 , C12N2310/14 , C12N2320/12 , C12Y114/19001 , C12Y207/01137 , C12Y207/11025 , C12Y301/04004 , C12Y402/01001 , G01N2510/00
摘要： The present invention is directed to methods of identifying agents useful for inhibiting cancer cells. The agents bind to one of the proteins described herein, or to a gene or mRNA encoding such protein. The invention is also directed to methods for inhibiting cancer cells by administering an agent that binds to one of the proteins described herein, or to a gene or mRNA encoding such protein.
摘要翻译：本发明涉及鉴定可用于抑制癌细胞的药剂的方法。这些试剂与本文所述的一种蛋白质结合，或与编码这种蛋白质的基因或mRNA结合。本发明还涉及通过施用与本文所述的蛋白质结合的试剂或编码这种蛋白质的基因或mRNA来抑制癌细胞的方法。

8. 发明专利

CA2515688A1 SIRNA LIBRARIES OPTIMIZED FOR PREDETERMINED PROTEIN FAMILIES 未知
公开(公告)号：CA2515688A1
公开(公告)日：2004-08-26
申请号：CA2515688
申请日：2004-02-10
申请人： IMMUSOL INC
发明人： LI HENRY , WONG-STAAL FLOSSIE , CHATTERTON JON E , FAN WUFANG , KE NING
IPC分类号： C12N15/11 , C12N15/113 , C07H21/00
摘要： siRNA Libraries Optimized for Predetermined Protein Families Libraries for generating small inhibitory RNA (siRNA) are provided where the members of the library are optimized to inhibit the expression of genes that encode a predetermined family of proteins. The members of the library target at least mRNA encoding all members of the family of proteins. Methods for generating siRNA libraries of the present invention are also provided.

9. 发明专利

AU2003254151A1 NOVEL SIRNA GENE LIBRARIES AND METHODS FOR THEIR PRODUCTION AND USE 未知
公开(公告)号：AU2003254151A1
公开(公告)日：2004-02-09
申请号：AU2003254151
申请日：2003-07-23
申请人： IMMUSOL INC
发明人： KE NING , WONG-STAAL FLOSSIE , LI HENRY , CHATTERTON JON E
IPC分类号： C12N15/09 , C12N15/10 , C12N15/11
摘要： The present invention relates to methods and compositions for the elucidation of gene function and the identification of novel genes. Specifically, the present invention relates to methods and compositions for improved functional genomic screening, functional inactivation of specific essential or non-essential genes, and identification of genes that are modulated in response to specific stimuli or encode recognizable phenotypic traits. In particular, the compositions of the present invention include, but are not limited to, expression cassettes comprising a novel dual promoter transcription system, that utilizes modified promoters, preferably containing complementary termination sequences, positioned across a coding sequence and in opposite orientation to each other. In addition, the present invention includes libraries comprising the expression cassettes of the invention, including vectors for transforming cells, such as replication-deficient retroviral vectors. The invention also includes methods for the production and screening of dsRNA/siRNA libraries, as well as therapeutic uses for the siRNAs expressed in accordance with the invention.

10. 发明专利

CA2493564A1 NOVEL SIRNA LIBRARIES AND THEIR PRODUCTION AND USE 未知
公开(公告)号：CA2493564A1
公开(公告)日：2004-01-29
申请号：CA2493564
申请日：2003-07-23
申请人： IMMUSOL INC
发明人： WONG-STAAL FLOSSIE , KE NING , CHATTERTON JON E , LI HENRY
IPC分类号： C12N15/09 , C12N15/10 , C12N15/11 , C12N15/74 , C12Q1/68
摘要： The present invention relates to methods and compositions for the elucidatio n of gene function and the identification of novel genes. Specifically, the present invention relates to methods and compositions for improved functiona l genomic screening, functional inactivation of specific essential or non- essential genes, and identification of genes that are modulated in response to specific stimuli or encode recognizable phenotypic traits. In particular, th e compositions of the present invention include, but are not limited to, expression cassettes comprising a novel dual promoter transcription system, that utilizes modified promoters, preferably containing complementary termination sequences, positioned across a coding sequence and in opposite orientation to each other. In addition, the present invention includes libraries comprising the expression cassettes of the invention, including vectors for transforming cells, such as replication-deficient retroviral vectors. The invention also includes methods for the production and screenin g of dsRNA/siRNA libraries, as well as therapeutic uses for the siRNAs express ed in accordance with the invention.

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