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2. 发明申请

US20150017738A1 MEANS AND METHODS OF MEASURING PARATHYROID HORMONE IN PATIENTS SUFFERING FROM OXIDATIVE STRESS 审中-公开
标题翻译：在氧化应激患者中测量PARATHYROID HORMONE的方法和方法
公开(公告)号：US20150017738A1
公开(公告)日：2015-01-15
申请号：US14380147
申请日：2013-02-22
申请人： IMMUNDIAGNOSTIK AG
发明人： Franz Paul Armbruster , Berthold Hocher , Hans Juergen Groen , Heinz Juergen Roth
IPC分类号： G01N33/78 , C07K16/26
CPC分类号： G01N33/78 , C07K16/26 , C07K2317/30 , C07K2317/34 , G01N33/6848 , G01N33/6854 , G01N33/6893 , G01N2333/635 , G01N2500/00 , G01N2800/046 , G01N2800/347 , G01N2800/7009
摘要： Method for obtaining an antibody or antibody fragment to a conformational epitope specific for oxidized, inactive human parathyroid hormone and fragments thereof; a method for removal of oxidized, inactive human parathyroid hormone from a sample of body fluid; methods of determining the concentration of active parathyroid hormone in a sample, and an in vitro method of diagnosis of renal failure or secondary hyperthyroidism in patients on dialysis. The antibody is obtained by immunizing an animal with an immunogen containing parathyroid hormone or fragment thereof oxidized at methionines at positions 8, 18 or both; and a recovering of antibodies; whereby the complementary determining region of the antibody or antibody fragment or single chain antibody specifically recognizes a conformational epitope (antigenic determinant) which is a tertiary structure and only present on oxidized parathyroid hormone and fragments thereof only but not regular bioactive human parathyroid hormone, while the binding surface to the complementary binding region does not include any one of the oxidized methionines of human parathyroid hormone.
摘要翻译：获得针对氧化的，无活性的人甲状旁腺激素及其片段特异的构象表位的抗体或抗体片段的方法; 从体液样品中去除氧化的，无活性的人甲状旁腺激素的方法; 确定样品中活性甲状旁腺激素浓度的方法，以及透析患者肾衰竭或继发性甲状腺功能亢进的体外诊断方法。抗体是通过用含有甲状旁腺激素或其片段的蛋白质在8,18或两者氧化的免疫原免疫动物获得的; 和抗体的回收; 由此抗体或抗体片段或单链抗体的互补决定区域特异性识别作为三级结构的构象表位（抗原决定簇），仅存在于氧化的甲状旁腺激素及其片段但不存在于规则的生物活性人甲状旁腺激素上，而与互补结合区的结合表面不包括人甲状旁腺激素的氧化甲硫氨酸中的任一种。

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