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1. 发明申请

WO2021180724A1 IMPROVEMENTS IN NUCLEIC ACID SEQUENCING 审中-公开
公开(公告)号：WO2021180724A1
公开(公告)日：2021-09-16
申请号：PCT/EP2021/055922
申请日：2021-03-09
申请人： ILLUMINA, INC.
发明人： PEACE, Jared , MONTANO, Jeanine , NIZIOLEK, Michael , SILBERGLEIT, Ark , CAPEK, Petr , MCINERNEY, Peter
IPC分类号： C12Q1/6806 , C12Q1/6837 , C12Q1/6869
摘要： A method of preparing templates for high throughput nucleic acid sequencing, comprising: a) providing a solid support, wherein said solid support comprises a plurality of nucleic acid primers immobilised adaptor nucleic acid sequences which hybridise to one or more of the nucleic acid primers, and the library preparation having a nucleic acid concentration of 400 pM or less;c) allowing single stranded fragments of template nucleic acid to bind to said nucleic acid primers, thereby immobilising said single stranded fragments on said solid support; and d) repeating steps b) and c) at least three further times, i.e. multiple loadings or rounds of template hybridization to improve occupation rates, e.g., of nanowells on a flow cell.

2. 发明申请

WO2017176896A1 METHODS AND SYSTEMS FOR CONSTRUCTION OF NORMALIZED NUCLEIC ACID LIBRARIES 审中-公开
标题翻译：用于构建正常化核酸文库的方法和系统
公开(公告)号：WO2017176896A1
公开(公告)日：2017-10-12
申请号：PCT/US2017/026169
申请日：2017-04-05
申请人： ILLUMINA, INC.
发明人： ZHANG, Zhihong , GAO, Yangbin , ECKHARDT, Allen, E. , CAPEK, Petr
IPC分类号： C12Q1/68 , C12N15/10 , B01L3/00
摘要： The present disclosure generally relates to nucleic acid amplification systems and methods suitable for construction of nucleic acid samples, including construction of normalized nucleic acid libraries. In some embodiments, the method includes providing one or more input nucleic acid samples, contacting each of the input nucleic acid samples (e.g., input library) with a reaction mixture including first amplification or normalization primers and second amplification or normalization primers, wherein the first amplification or normalization primers are immobilized on a solid support and the second amplification or normalization primers are in solution phase, and amplifying the input nucleic acid samples under conditions such that substantially all of the first amplification or normalization primers are incorporated into amplification products. Further provided are systems and droplet actuator devices that are configured to carry out the methods disclosed herein. Compositions that include nucleic acid samples and libraries, preferably normalized, prepared in accordance with the disclosed methods and systems are also provided.
摘要翻译：本公开总体上涉及适用于构建核酸样品的核酸扩增系统和方法，包括构建标准化的核酸文库。在一些实施方案中，该方法包括提供一个或多个输入核酸样品，使每个输入核酸样品（例如输入文库）与包含第一扩增或标准化引物和第二扩增或标准化引物的反应混合物接触，其中第一扩增或标准化引物固定在固体支持物上，并且第二扩增或标准化引物处于溶液相中，并且在使得基本上所有第一扩增或标准化引物掺入扩增产物的条件下扩增输入核酸样品。进一步提供了被配置为执行本文公开的方法的系统和液滴致动器装置。还提供了包含核酸样品和文库的组合物，优选标准化，根据所公开的方法和系统制备。

3. 发明申请

WO2016182814A2 CATIONIC POLYMERS AND METHOD OF SURFACE APPLICATION 审中-公开
标题翻译：阳离子聚合物和表面应用方法
公开(公告)号：WO2016182814A2
公开(公告)日：2016-11-17
申请号：PCT/US2016/030742
申请日：2016-05-04
申请人： ILLUMINA, INC.
发明人： ECKHARDT, Allen E. , PANTOJA, Rijo , RAMIREZ, Sean M. , CAPEK, Petr , LI, Edwin
IPC分类号： B01L3/00
CPC分类号： B01L3/502707 , B01L3/502784 , B01L2200/0673 , B01L2200/141 , B01L2300/0645 , B01L2300/0816 , B01L2300/0887 , B01L2300/163 , B01L2400/0427
摘要： Embodiments of present application are directed to microfluidic devices and particularly digital microfluidic devices that are specifically designed to prevent sample contamination during sample processing, methods of manufacturing the same, and methods to improve sample analysis process by preventing sample contamination.
摘要翻译：本申请的实施例涉及微流体装置，特别是专门设计用于防止样品处理期间样品污染的数字微流体装置，其制造方法，以及通过防止样品污染来改进样品分析过程的方法。

4. 发明公开

EP3440220A1 METHODS AND SYSTEMS FOR CONSTRUCTION OF NORMALIZED NUCLEIC ACID LIBRARIES 有权
公开(公告)号：EP3440220A1
公开(公告)日：2019-02-13
申请号：EP17718279.7
申请日：2017-04-05
申请人： Illumina, Inc.
发明人： ZHANG, Zhihong , GAO, Yangbin , ECKHARDT, Allen, E. , CAPEK, Petr
IPC分类号： C12Q1/68 , C12N15/10 , B01L3/00
摘要： The present disclosure generally relates to nucleic acid amplification systems and methods suitable for construction of nucleic acid samples, including construction of normalized nucleic acid libraries. In some embodiments, the method includes providing one or more input nucleic acid samples, contacting each of the input nucleic acid samples (e.g., input library) with a reaction mixture including first amplification or normalization primers and second amplification or normalization primers, wherein the first amplification or normalization primers are immobilized on a solid support and the second amplification or normalization primers are in solution phase, and amplifying the input nucleic acid samples under conditions such that substantially all of the first amplification or normalization primers are incorporated into amplification products. Further provided are systems and droplet actuator devices that are configured to carry out the methods disclosed herein. Compositions that include nucleic acid samples and libraries, preferably normalized, prepared in accordance with the disclosed methods and systems are also provided.

5. 发明公开

EP3969612A1 IMPROVEMENTS IN NUCLEIC ACID SEQUENCING 审中-实审
公开(公告)号：EP3969612A1
公开(公告)日：2022-03-23
申请号：EP21711819.9
申请日：2021-03-09
申请人： Illumina, Inc.
发明人： PEACE, Jared , MONTANO, Jeanine , NIZIOLEK, Michael , SILBERGLEIT, Ark , CAPEK, Petr , MCINERNEY, Peter
IPC分类号： C12Q1/6806 , C12Q1/6837 , C12Q1/6869

6. 发明授权

EP3440220B1 METHODS AND SYSTEMS FOR CONSTRUCTION OF NORMALIZED NUCLEIC ACID LIBRARIES 有权
公开(公告)号：EP3440220B1
公开(公告)日：2020-03-25
申请号：EP17718279.7
申请日：2017-04-05
申请人： Illumina, Inc.
发明人： ZHANG, Zhihong , GAO, Yangbin , ECKHARDT, Allen, E. , CAPEK, Petr
IPC分类号： C12Q1/6844

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