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1. 发明申请

US20120292184A1 MICROBIAL IDENTIFICATION AND MANIPULATION OF NANOSCALE BIOMOLECULES 有权
标题翻译：纳米生物分子的微生物鉴定和操作
公开(公告)号：US20120292184A1
公开(公告)日：2012-11-22
申请号：US13108216
申请日：2011-05-16
申请人： I-Fang CHENG , Hsien-Chang Chang , Cheng-Che Chung
发明人： I-Fang CHENG , Hsien-Chang Chang , Cheng-Che Chung
IPC分类号： G01N27/447 , C25B15/00 , B82Y40/00
CPC分类号： C12Q1/6816 , B03C5/005 , B03C5/026 , C12N13/00 , G01N33/536 , G01N33/558 , G01N33/569 , G01N33/582 , G01N33/583 , C12Q2565/629
摘要： A method of microbial identification is disclosed. The method includes the steps of assembling dielectrophoretic particles modified with specific DNA probes on a surface thereof in a continuous fluid at a predetermined location in a microchannel to form a particle assembly by a negative dielectrophoretic force and a hydrodynamic force provided by the continuous fluid, narrowing gaps between the dielectrophoretic particles of the particle assembly to enhance the electric field in the gaps between the dielectrophoretic particles, injecting a fluid containing target DNAs of a target microbe into the microchannel at a predetermined flow rate to move the target DNAs toward the particle assembly and generating a positive dielectrophoretic force by the enhanced electric field to attract the target DNAs toward the dielectrophoretic particles of the particle assembly for hybridization with the DNA probes. The present invention also discloses a method of manipulation of nanoscale biomolecules.
摘要翻译：公开了一种微生物识别方法。该方法包括以下步骤：在微通道中的预定位置处将表面上的特定DNA探针修饰的介电泳细粒组装在微通道中的预定位置处，以通过负介电电泳力和由连续流体提供的流体动力力形成粒子组件颗粒组件的介电电泳颗粒之间的间隙以增强介电电泳颗粒之间的间隙中的电场，以预定流速将含有目标微生物的目标DNA的流体注入微通道以将靶DNA移动到颗粒组件，并且通过增强的电场产生正的介电电泳力，以将靶DNA吸引到颗粒组件的介电电泳颗粒，以与DNA探针杂交。本发明还公开了一种操作纳米尺度生物分子的方法。

2. 发明授权

US08911605B2 Method for antibiotic susceptibility testing and determining minimum inhibitory concentration of the antibiotic 有权
标题翻译：抗生素敏感性检测方法，并确定抗生素的最小抑菌浓度
公开(公告)号：US08911605B2
公开(公告)日：2014-12-16
申请号：US13473717
申请日：2012-05-17
申请人： Cheng-Che Chung , I-Fang Cheng , Wen-Horng Yang , Hsien-Chang Chang
发明人： Cheng-Che Chung , I-Fang Cheng , Wen-Horng Yang , Hsien-Chang Chang
IPC分类号： G01N27/447 , G01N33/15 , C12Q1/18
CPC分类号： G01N33/15 , B03C5/005 , C12Q1/18
摘要： A method of antibiotic susceptibility testing is disclosed, and includes the following steps: (A) providing a sample to be tested wherein the sample contains a microbe; (B) adding an antibiotic into the sample, wherein the antibiotic serves to inhibit cell wall synthesis; (C) checking the sample by dielectrophoresis and observing a shape change of the microbe; and (D) determining whether the microbe is susceptible to the antibiotic according to the shape change thereof. The present invention also discloses a method for determining a minimum inhibitory concentration of the antibiotic.
摘要翻译：公开了一种抗生素敏感性试验方法，包括以下步骤：（A）提供样品，其中样品含有微生物; （B）将抗生素添加到样品中，其中抗生素用于抑制细胞壁合成; （C）通过介电电泳检查样品并观察微生物的形状变化; 和（D）根据其形状变化确定微生物是否易受抗生素的影响。本发明还公开了一种确定抗生素最小抑制浓度的方法。

3. 发明授权

US08808518B2 Microbial identification and manipulation of nanoscale biomolecules 有权
标题翻译：微生物鉴定和纳米级生物分子的操作
公开(公告)号：US08808518B2
公开(公告)日：2014-08-19
申请号：US13108216
申请日：2011-05-16
申请人： I-Fang Cheng , Hsien-Chang Chang , Cheng-Che Chung
发明人： I-Fang Cheng , Hsien-Chang Chang , Cheng-Che Chung
IPC分类号： B01D57/02 , G01N33/536 , G01N33/58 , B03C5/02 , G01N33/569 , G01N33/558 , C12N13/00 , C12Q1/68 , B03C5/00
CPC分类号： C12Q1/6816 , B03C5/005 , B03C5/026 , C12N13/00 , G01N33/536 , G01N33/558 , G01N33/569 , G01N33/582 , G01N33/583 , C12Q2565/629
摘要： A method of microbial identification is disclosed. The method includes the steps of assembling dielectrophoretic particles modified with specific DNA probes on a surface thereof in a continuous fluid at a predetermined location in a microchannel to form a particle assembly by a negative dielectrophoretic force and a hydrodynamic force provided by the continuous fluid, narrowing gaps between the dielectrophoretic particles of the particle assembly to enhance the electric field in the gaps between the dielectrophoretic particles, injecting a fluid containing target DNAs of a target microbe into the microchannel at a predetermined flow rate to move the target DNAs toward the particle assembly and generating a positive dielectrophoretic force by the enhanced electric field to attract the target DNAs toward the dielectrophoretic particles of the particle assembly for hybridization with the DNA probes. The present invention also discloses a method of manipulation of nanoscale biomolecules.
摘要翻译：公开了一种微生物识别方法。该方法包括以下步骤：在微通道中的预定位置处将表面上的特定DNA探针修饰的介电泳细粒组装在微通道中的预定位置处，以通过负介电电泳力和由连续流体提供的流体动力力形成粒子组件颗粒组件的介电电泳颗粒之间的间隙以增强介电电泳颗粒之间的间隙中的电场，以预定流速将含有目标微生物的目标DNA的流体注入微通道以将靶DNA移动到颗粒组件，并且通过增强的电场产生正的介电电泳力，以将靶DNA吸引到颗粒组件的介电电泳颗粒，以与DNA探针杂交。本发明还公开了一种操作纳米尺度生物分子的方法。

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