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1. 发明授权

US08715965B2 Method of amplifying nucleic acid from blood 有权
标题翻译：从血液中扩增核酸的方法
公开(公告)号：US08715965B2
公开(公告)日：2014-05-06
申请号：US11746722
申请日：2007-05-10
申请人： Hun-joo Lee , Sung-young Jeong , Joon-ho Kim , Kyu-youn Hwang
发明人： Hun-joo Lee , Sung-young Jeong , Joon-ho Kim , Kyu-youn Hwang
IPC分类号： C12Q1/68 , C12P19/34 , G01N27/44
CPC分类号： B01D61/422 , B01D2325/20 , C12Q1/6806 , C12Q2565/629
摘要： A method of amplifying nucleic acid from blood, the method including performing an electrodialysis on a blood sample to reduce the ionic strength of the sample, and performing a Polymerase Chain Reaction (“PCR”) using the blood sample on which the electrodialysis is performed as a template.
摘要翻译：一种从血液中扩增核酸的方法，所述方法包括对血液样品进行电渗析以降低样品的离子强度，并使用进行电渗析的血液样品进行聚合酶链式反应（“PCR”），一个模板

2. 发明授权

US07402390B2 Method of isolating nucleic acid using material positively charged at first pH and containing amino group and carboxyl group 有权
标题翻译：使用在第一个pH下带正电并含有氨基和羧基的物质分离核酸的方法
公开(公告)号：US07402390B2
公开(公告)日：2008-07-22
申请号：US11317949
申请日：2005-12-23
申请人： Kyu-youn Hwang , Joon-ho Kim , Chang-eun Yoo , Hun-joo Lee , Hee-kyun Lim , Sung-young Jeong , Jeo-young Shim
发明人： Kyu-youn Hwang , Joon-ho Kim , Chang-eun Yoo , Hun-joo Lee , Hee-kyun Lim , Sung-young Jeong , Jeo-young Shim
IPC分类号： C12Q1/68 , G01N33/53 , C07H21/00
CPC分类号： C12N15/1006 , B01D15/361 , B01D15/364 , B01J20/3285 , B01J43/00 , C12Q1/6806 , C12Q2527/119 , C12Q2523/308
摘要： A method of isolating nucleic acid from a sample containing nucleic acid is provided. The method includes contacting the sample with a bifunctional material that contains an amino group and a carboxyl group and is positively charged at a first pH to allow binding of the nucleic acid to the bifunctional material; and extracting the nucleic acid at a second pH higher than the first pH from the complex.
摘要翻译：提供了从含有核酸的样品中分离核酸的方法。该方法包括使样品与含有氨基和羧基的双官能材料接触并在第一pH下带正电荷以允许核酸与双功能材料的结合; 以及从所述络合物中提取比所述第一pH高的第二pH的所述核酸。

3. 发明申请

US20080044884A1 METHOD AND DEVICE FOR SEPARATING CELLS FROM A SAMPLE USING A NONPLANAR SOLID SUBSTRATE 审中-公开
标题翻译：使用非固体基质从样品分离细胞的方法和装置
公开(公告)号：US20080044884A1
公开(公告)日：2008-02-21
申请号：US11841089
申请日：2007-08-20
申请人： Kyu-youn Hwang , Sung-young Jeong , Joon-ho Kim , Hun-joo Lee , Jung-im Han
发明人： Kyu-youn Hwang , Sung-young Jeong , Joon-ho Kim , Hun-joo Lee , Jung-im Han
IPC分类号： C12N7/02 , C12M1/00 , C12N1/00 , C12N1/20
CPC分类号： C12N15/1006 , B01L7/52 , B01L2300/0636 , C12N2533/30 , C12Q1/6806
摘要： Provided is a method and device for separating a cell from a sample. The method comprises contacting a nonplanar solid substrate with a cell-containing sample in a liquid medium having a pH of 3.0 to 6.0.
摘要翻译：提供了用于从样品中分离细胞的方法和装置。该方法包括使非平面固体基质与含有细胞的样品在pH为3.0至6.0的液体培养基中接触。

4. 发明申请

US20070281307A1 METHOD OF AMPLIFYING NUCLEIC ACID FROM BLOOD 有权
标题翻译：从血液中放大核酸的方法
公开(公告)号：US20070281307A1
公开(公告)日：2007-12-06
申请号：US11746722
申请日：2007-05-10
申请人： Hun-joo Lee , Sung-young Jeong , Joon-ho Kim , Kyu-youn Hwang
发明人： Hun-joo Lee , Sung-young Jeong , Joon-ho Kim , Kyu-youn Hwang
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： B01D61/422 , B01D2325/20 , C12Q1/6806 , C12Q2565/629
摘要： A method of amplifying nucleic acid from blood, the method including performing an electrodialysis on a blood sample to reduce the ionic strength of the sample, and performing a Polymerase Chain Reaction (“PCR”) using the blood sample on which the electrodialysis is performed as a template.
摘要翻译：一种从血液中扩增核酸的方法，所述方法包括对血液样品进行电渗析以降低样品的离子强度，并使用进行电渗析的血液样品进行聚合酶链式反应（“PCR”），一个模板

5. 发明申请

US20060154284A1 Method of isolating nucleic acid using material positively charged at first pH and containing amino group and carboxyl group 有权
标题翻译：使用在第一个pH下带正电并含有氨基和羧基的物质分离核酸的方法
公开(公告)号：US20060154284A1
公开(公告)日：2006-07-13
申请号：US11317949
申请日：2005-12-23
申请人： Kyu-youn Hwang , Joon-ho Kim , Chang-eun Yoo , Hun-joo Lee , Hee-kyun Lim , Sung-young Jeong , Jeo-young Shim
发明人： Kyu-youn Hwang , Joon-ho Kim , Chang-eun Yoo , Hun-joo Lee , Hee-kyun Lim , Sung-young Jeong , Jeo-young Shim
IPC分类号： C12Q1/68 , C07H21/04
CPC分类号： C12N15/1006 , B01D15/361 , B01D15/364 , B01J20/3285 , B01J43/00 , C12Q1/6806 , C12Q2527/119 , C12Q2523/308
摘要： A method of isolating nucleic acid from a sample containing nucleic acid is provided. The method includes contacting the sample with a bifunctional material that contains an amino group and a carboxyl group and is positively charged at a first pH to allow binding of the nucleic acid to the bifunctional material; and extracting the nucleic acid at a second pH higher than the first pH from the complex.
摘要翻译：提供了从含有核酸的样品中分离核酸的方法。该方法包括使样品与含有氨基和羧基的双官能材料接触并在第一pH下带正电荷以允许核酸与双功能材料的结合; 以及从所述络合物中提取比所述第一pH高的第二pH的所述核酸。

6. 发明授权

US07955842B2 Microfluidic device and method for concentrating sample containing cells or viruses and lysing cells or viruses, and method of producing the microfluidic device 有权
标题翻译：用于浓缩含有细胞或病毒的样品和裂解细胞或病毒的微流体装置和方法以及产生微流体装置的方法
公开(公告)号：US07955842B2
公开(公告)日：2011-06-07
申请号：US11670002
申请日：2007-02-01
申请人： Hun-joo Lee , Joon-ho Kim , Sung-young Jeong , Kyu-youn Hwang
发明人： Hun-joo Lee , Joon-ho Kim , Sung-young Jeong , Kyu-youn Hwang
IPC分类号： C12M1/42 , C12M1/33
CPC分类号： C12M47/02 , C12M47/06
摘要： A microfluidic device for concentrating a sample including cells or viruses and lysing the cells or viruses, the device including an anode chamber including an anode electrode, a cathode chamber including a cathode electrode and an ion exchange membrane separating the anode chamber and the cathode chamber. The cathode chamber includes a solid support therein. A method of producing the microfluidic device and a method of concentrating a sample including cells or viruses and lysing the cells or viruses therein using the microfluidic device.
摘要翻译：一种用于浓缩包含细胞或病毒的样品并裂解细胞或病毒的微流体装置，所述装置包括包括阳极电极的阳极室，包括阴极的阴极室和分离阳极室和阴极室的离子交换膜。阴极室包括固体支撑体。微流体装置的制造方法以及浓缩包含细胞或病毒的样品并使用微流体装置使细胞或病毒裂解的方法。

7. 发明授权

US07919278B2 Method of amplifying nucleic acid from a cell using a nonplanar solid substrate 有权
标题翻译：使用非平面固体底物从细胞中扩增核酸的方法
公开(公告)号：US07919278B2
公开(公告)日：2011-04-05
申请号：US11841107
申请日：2007-08-20
申请人： Sung-young Jeong , Kyu-youn Hwang , Joon-ho Kim , Jung-im Han , Hun-joo Lee
发明人： Sung-young Jeong , Kyu-youn Hwang , Joon-ho Kim , Jung-im Han , Hun-joo Lee
IPC分类号： C12P19/34 , C12Q1/68
CPC分类号： C12N15/1006 , B01L7/52 , B01L2300/0636 , C12N2533/30 , C12Q1/6806
摘要： Provided is a method of amplifying nucleic acid from a comprising: contacting a cell-containing sample with a nonplanar solid substrate in a liquid medium having a pH range of 3.0-6.0 to attach the cell to the solid substrate; washing the nonplanar solid substrate to remove materials that are not attached thereto; and performing PCR using the nucleic acid from the cell attached to the nonplanar solid substrate as a template sample to amplify nucleic acid from the cell, wherein the contacting, washing and PCR processes are performed in a single vessel.
摘要翻译：本发明提供一种扩增来自a的核酸的方法，其特征在于：在pH范围为3.0-6.0的液体介质中使含细胞的样品与非平面固体基质接触，以将该细胞附着在固体基质上; 洗涤非平面固体基质以除去未附着于其上的材料; 以及使用来自附着在所述非平面固体底物上的细胞的核酸作为模板样品进行PCR以扩增来自所述细胞的核酸，其中所述接触，洗涤和PCR过程在单个容器中进行。

8. 发明授权

US07695678B2 Method of isolating nucleic acid using material positively charged at first pH and containing amino group and carboxyl group 有权
标题翻译：使用在第一个pH下带正电并含有氨基和羧基的物质分离核酸的方法
公开(公告)号：US07695678B2
公开(公告)日：2010-04-13
申请号：US12141894
申请日：2008-06-18
申请人： Kyu-youn Hwang , Joon-ho Kim , Chang-eun Yoo , Hun-joo Lee , Hee-kyun Lim , Sung-yung Jeong , Jeo-young Shim
发明人： Kyu-youn Hwang , Joon-ho Kim , Chang-eun Yoo , Hun-joo Lee , Hee-kyun Lim , Sung-yung Jeong , Jeo-young Shim
IPC分类号： G01N15/06 , A01N61/00 , C12Q1/68 , G01N33/53 , C07H21/04
CPC分类号： C12N15/1006 , B01D15/361 , B01D15/364 , B01J20/3285 , B01J43/00 , C12Q1/6806 , C12Q2527/119 , C12Q2523/308
摘要： A method of isolating nucleic acid from a sample containing nucleic acid is provided. The method includes contacting the sample with a bifunctional material that contains an amino group and a carboxyl group and is positively charged at a first pH to allow binding of the nucleic acid to the bifunctional material; and extracting the nucleic acid at a second pH higher than the first pH from the complex.
摘要翻译：提供了从含有核酸的样品中分离核酸的方法。该方法包括使样品与含有氨基和羧基的双官能材料接触并在第一pH下带正电荷以允许核酸与双功能材料的结合; 以及从所述络合物中提取比所述第一pH高的第二pH的所述核酸。

9. 发明申请

US20060134777A1 Microfluidic device comprising electrolysis device for cell lysis and method for electrochemically lysing cells using the same 有权
公开(公告)号：US20060134777A1
公开(公告)日：2006-06-22
申请号：US11304083
申请日：2005-12-15
申请人： Hun-joo Lee , Joon-ho Kim , Chang-eun Yoo , Hee-kyun Lim , Kyu-youn Hwang , Soo-min Ma , Jun-hong Min
发明人： Hun-joo Lee , Joon-ho Kim , Chang-eun Yoo , Hee-kyun Lim , Kyu-youn Hwang , Soo-min Ma , Jun-hong Min
IPC分类号： G01N27/447 , C12M1/33
CPC分类号： C12N1/066 , C12M47/06
摘要： Provided are a microfluidic device including an electrolysis device for cell lysis which includes an anode chamber, a cathode chamber and a separator, in which the separator is installed between the anode chamber and the cathode chamber, the anode chamber includes an inlet and an outlet for an anode chamber solution and an electrode, and the cathode chamber includes an inlet and an outlet for a cathode chamber solution and an electrode, and a method of electrochemically lysing cells using the same.

10. 发明授权

US07914660B2 Method of controlling pH of electrolyzed solution using microfluidic device including electrolysis device 有权
标题翻译：使用包括电解装置的微流体装置控制电解液pH值的方法
公开(公告)号：US07914660B2
公开(公告)日：2011-03-29
申请号：US11303673
申请日：2005-12-16
申请人： Hun-joo Lee , Joon-ho Kim , Chang-eun Yoo , Kyu-youn Hwang , Hee-kyun Lim
发明人： Hun-joo Lee , Joon-ho Kim , Chang-eun Yoo , Kyu-youn Hwang , Hee-kyun Lim
IPC分类号： C25B1/10
CPC分类号： G05D21/02 , B01D61/422 , B01L3/50273 , B01L2200/14 , B01L2300/0867 , B01L2400/0415 , C02F1/4618 , C02F2103/02 , C02F2201/4611 , C02F2201/46115 , C02F2201/4618 , C02F2209/06 , Y02E60/366
摘要： Provided is a method of controlling the pH of a solution using electrolysis in a microfluidic device comprising an electrolysis device including an anode chamber, a cathode chamber, and a partition membrane between the anode chamber and the cathode chamber, wherein the anode chamber includes an inlet and an outlet through which an anode chamber solution enters and is discharged from the anode chamber, respectively, and an electrode, and the cathode chamber includes an inlet and an outlet through which a cathode chamber solution enters and is discharged from the cathode chamber, respectively, and an electrode. The method includes: flowing the anode chamber solution containing a compound having a lower standard oxidation potential than water into the anode chamber through the inlet of the anode chamber; flowing the cathode chamber solution containing a compound having a lower standard reduction potential than water into the cathode chamber through the inlet of the cathode chamber; applying voltage between the electrode in the anode chamber and the electrode in the cathode chamber to induce electrolysis in the anode chamber and the cathode chamber; and mixing equal volumes of the acidic anode-electrolyzed solution and the basic cathode-electrolyzed solution, wherein the volumes of the cathode chamber and the anode chamber are controlled in a predetermined ratio according to a target pH of a solution obtained by mixing the acidic anode-electrolyzed solution and the basic cathode-electrolyzed solution.
摘要翻译：提供了一种在微流体装置中使用电解来控制溶液的pH的方法，该微流体装置包括在阳极室和阴极室之间包括阳极室，阴极室和分隔膜的电解装置，其中阳极室包括入口以及阳极室溶液分别从阳极室进入和排出的出口以及电极，阴极室包括入口和出口，阴极室溶液分别从阴极室溶液进入并从阴极室排出，和电极。该方法包括：将含有比水低的标准氧化电位的化合物的阳极室溶液通过阳极室的入口流入阳极室; 将含有比水低的标准还原电位的化合物的阴极室溶液通过阴极室的入口流入阴极室; 在阳极室中的电极和阴极室中的电极之间施加电压以在阳极室和阴极室中引起电解; 并混合等体积的酸性阳极电解溶液和碱性阴极电解溶液，其中阴极室和阳极室的体积根据通过将酸性阳极混合获得的溶液的目标pH控制在预定比例电解液和碱性阴极电解溶液。

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