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1. 发明授权

US5696157A Sulfonated derivatives of 7-aminocoumarin 失效
标题翻译： 7-氨基香豆素的磺化衍生物
公开(公告)号：US5696157A
公开(公告)日：1997-12-09
申请号：US749753
申请日：1996-11-15
申请人： Hui-Ying Wang , Wai-Yee Leung , Fei Mao
发明人： Hui-Ying Wang , Wai-Yee Leung , Fei Mao
IPC分类号： C07D311/16 , C07D405/12 , A61K31/37 , C07D311/18
CPC分类号： C07D405/12 , C07D311/16
摘要： The present invention describes 7-aminocoumarin dyes that are substituted one or more times at the 3-, 6- and/or 8-positions by a sulfonic acid or a salt of a sulfonic acid, said dyes being useful as fluorescent probes or in the preparation of enzyme substrates, caged probes, or adducts with reducing sugars. The dyes of the invention optionally possess a reactive group useful for preparing fluorescent conjugates, which conjugates and methods for their preparation are described herein.
摘要翻译：本发明描述了在3-，6-和/或8位被磺酸或磺酸盐取代一次或多次的7-氨基香豆素染料，所述染料可用作荧光探针或在酶底物的制备，笼式探针或与还原糖的加合物。本发明的染料任选地具有可用于制备荧光共轭物的反应性基团，其缀合物及其制备方法在本文中描述。

2. 发明授权

US08877437B1 Methods of using dyes in association with nucleic acid staining or detection 有权
标题翻译：使用染料与核酸染色或检测相关的方法
公开(公告)号：US08877437B1
公开(公告)日：2014-11-04
申请号：US12976917
申请日：2010-12-22
申请人： Fei Mao , Wai-Yee Leung , Tam Van
发明人： Fei Mao , Wai-Yee Leung , Tam Van
IPC分类号： C12Q1/68 , C12Q1/02 , C12P19/24 , C07H21/02 , C07H21/04 , C07H19/04
CPC分类号： C12Q1/6816 , C12Q2563/173
摘要： Methods of using dyes and associated technology are provided. A dye, such as a monomeric dye or a dimeric dye, may be used in a nucleic acid gel staining application and/or a nucleic acid detection application. A dimeric dye, such as a dimeric dye capable of forming a hairpin-like structure, may be used to stain and/or detect nucleic acids via a release-on-demand mechanism. A dimeric dye having low background fluorescence in the absence of nucleic acids and high fluorescence in the presence of nucleic acids, upon binding therewith, may be used to stain and/or detect nucleic acids. Buffers comprising a weak acid and a salt of the weak acid, such as a lithium salt, are also provided to allow for effective prestaining of nucleic acids.
摘要翻译：提供了使用染料和相关技术的方法。可以在核酸凝胶染色应用和/或核酸检测应用中使用染料，例如单体染料或二聚染料。可以使用二聚染料，例如能够形成发夹状结构的二聚染料，以通过按需释放机制对核酸进行染色和/或检测。在存在核酸时具有低背景荧光的二聚染料和在存在核酸时与核酸存在时的高荧光，可以用于染色和/或检测核酸。还提供了包含弱酸和弱酸盐的缓冲剂，例如锂盐，以允许核酸的有效预染色。

3. 发明申请

US20120329068A1 FLUORESCENT DYES, FLUORESCENT DYE KITS, AND METHODS OF PREPARING LABELED MOLECULES 有权
标题翻译：荧光染料，荧光染发剂及其制备标记分子的方法
公开(公告)号：US20120329068A1
公开(公告)日：2012-12-27
申请号：US13423092
申请日：2012-03-16
申请人： Fei Mao , Wai-Yee Leung , Ching-Ying Cheung , Hye Eun Hoover
发明人： Fei Mao , Wai-Yee Leung , Ching-Ying Cheung , Hye Eun Hoover
IPC分类号： G01N21/64 , G01N33/53 , C12N9/96 , C07K1/113
CPC分类号： G01N1/30 , C07K1/13 , C07K17/06 , C09B11/24 , C09B23/06 , C09B23/083 , C09B57/001 , C09B57/02 , G01N33/53 , G01N33/532 , G01N33/533 , G01N33/582 , G01N33/6854
摘要： The present invention provides methods, compositions, and kits useful in preparing labeled molecules, which are useful in the detection of binding partners.
摘要翻译：本发明提供了可用于制备标记分子的方法，组合物和试剂盒，其可用于检测结合配偶体。

4. 发明申请

US20120282617A1 DETECTION USING A DYE AND A DYE MODIFIER 有权
标题翻译：使用染色剂和染色体改性剂进行检测
公开(公告)号：US20120282617A1
公开(公告)日：2012-11-08
申请号：US13413352
申请日：2012-03-06
申请人： Fei Mao , Wai-Yee Leung , Hye Eun Hoover
发明人： Fei Mao , Wai-Yee Leung , Hye Eun Hoover
IPC分类号： G01N21/64
CPC分类号： C09B67/0033 , C09B69/02 , Y10T436/143333
摘要： The present invention relates to dyes in general. The present invention provides a wide range of dyes and kits containing the same, which are applicable for labeling a variety of biomolecules such as nucleic acids, cells and microorganisms. The present invention also provides various methods of using the dyes for research and development, forensic identification, environmental studies, diagnosis, prognosis, and/or treatment of disease conditions.
摘要翻译：本发明一般涉及染料。本发明提供了广泛的含有该染料和试剂盒的染料和试剂盒，其适用于标记各种生物分子如核酸，细胞和微生物。本发明还提供了用于研究和开发，法医鉴定，环境研究，诊断，预后和/或治疗疾病状况的各种方法。

5. 发明授权

US08148515B1 Detection using a dye and a dye modifier 有权
标题翻译：使用染料和染料改性剂进行检测
公开(公告)号：US08148515B1
公开(公告)日：2012-04-03
申请号：US12792718
申请日：2010-06-02
申请人： Fei Mao , Wai-Yee Leung , Hye Eun Hoover
发明人： Fei Mao , Wai-Yee Leung , Hye Eun Hoover
IPC分类号： C07H21/00
CPC分类号： C09B67/0033 , C09B69/02 , Y10T436/143333
摘要： The present invention relates to dyes in general. The present invention provides a wide range of dyes and kits containing the same, which are applicable for labeling a variety of biomolecules such as nucleic acids, cells and microorganisms. The present invention also provides various methods of using the dyes for research and development, forensic identification, environmental studies, diagnosis, prognosis, and/or treatment of disease conditions.
摘要翻译：本发明一般涉及染料。本发明提供了广泛的含有该染料和试剂盒的染料和试剂盒，其适用于标记各种生物分子如核酸，细胞和微生物。本发明还提供了用于研究和开发，法医鉴定，环境研究，诊断，预后和/或治疗疾病状况的各种方法。

6. 发明授权

US07776567B2 Dimeric and trimeric nucleic acid dyes, and associated systems and methods 有权
标题翻译：二聚体和三聚体核酸染料，以及相关系统和方法
公开(公告)号：US07776567B2
公开(公告)日：2010-08-17
申请号：US11377253
申请日：2006-03-16
申请人： Fei Mao , Wai-Yee Leung , Xing Xin
发明人： Fei Mao , Wai-Yee Leung , Xing Xin
IPC分类号： C12P19/34 , C12Q1/68 , C07H19/04 , C07H21/02 , C07H21/04
CPC分类号： C12Q1/6876 , C07D209/08 , C07D213/38 , C07D221/10 , C07D417/06 , C07D417/14 , C07D491/147 , C07H21/00 , C09B15/00 , C12Q1/6851 , C12Q1/686 , C12Q2563/173
摘要： Dimeric and trimeric nucleic acid dyes, and associated systems and methods are provided. Such a dye may form a hairpin-like structure that enables it to stain nucleic acids via a release-on-demand mechanism, for example. Such a dye may have low background fluorescence in the absence of nucleic acids and high fluorescence in the presence of nucleic acids, upon binding therewith, for example. A dye provided herein may be useful in a variety of applications, such as in DNA quantitation in real-time PCR, for example.
摘要翻译：提供二聚和三聚体核酸染料以及相关系统和方法。这样的染料可以形成发夹状结构，使其能够通过例如按需释放机制来染色核酸。在不存在核酸的情况下，这样的染料可能具有低的背景荧光，并且例如在与核酸的结合时在核酸存在下具有高荧光。本文提供的染料可用于各种应用，例如在实时PCR中的DNA定量中。

7. 发明申请

US20100009454A1 METHODS OF USING DYES IN ASSOCIATION WITH NUCLEIC ACID STAINING OR DETECTION AND ASSOCIATED TECHNOLOGY 有权
标题翻译：使用染料与核酸染色或检测及相关技术相结合的方法
公开(公告)号：US20100009454A1
公开(公告)日：2010-01-14
申请号：US12484968
申请日：2009-06-15
申请人： Fei Mao , Wai-Yee Leung
发明人： Fei Mao , Wai-Yee Leung
IPC分类号： G01N33/50
CPC分类号： C09B15/00 , C09B11/28 , C09B23/04 , C12Q1/6818 , G01N1/30 , G01N21/6486 , Y10S435/81 , Y10T436/143333 , C12Q2525/301
摘要： Methods of using dyes and associated technology are provided. A dye, such as a monomeric dye or a dimeric dye, may be used in a nucleic acid gel staining application and/or a nucleic acid detection application. Such a dye and a salt that comprises an anion that is associated with a strong acid and a cation that is associated with a strong base may be used in such an application. A dimeric dye, such as a dimeric dye capable of forming a hairpin-like structure, may be used to stain and/or detect nucleic acids via a release-on-demand mechanism. A dimeric dye having low background fluorescence in the absence of nucleic acids and high fluorescence in the presence of nucleic acids, upon binding therewith, may be used to stain and/or detect nucleic acids.
摘要翻译：提供了使用染料和相关技术的方法。可以在核酸凝胶染色应用和/或核酸检测应用中使用染料，例如单体染料或二聚染料。这种染料和包含与强酸相关的阴离子和与强碱相关的阳离子的盐可用于这种应用中。可以使用二聚染料，例如能够形成发夹状结构的二聚染料，以通过按需释放机制对核酸进行染色和/或检测。在存在核酸时具有低背景荧光的二聚染料和在存在核酸时与核酸存在时的高荧光可用于染色和/或检测核酸。

8. 发明授权

US08790892B2 Substituted anthraquinone dyes for cellular stains and enzyme detection 有权
标题翻译：取代的蒽醌染料用于细胞染色和酶检测
公开(公告)号：US08790892B2
公开(公告)日：2014-07-29
申请号：US13439821
申请日：2012-04-04
申请人： Fei Mao , Wai-Yee Leung , Ching-Ying Cheung , Lori Michelle Roberts
发明人： Fei Mao , Wai-Yee Leung , Ching-Ying Cheung , Lori Michelle Roberts
IPC分类号： G01N1/30
CPC分类号： C09B1/516 , A61K49/0052 , C09B1/501 , C09B1/51 , C09B1/5155 , G01N1/30 , G01N21/6428
摘要： The invention discloses new substituted anthraquinone dyes that may be useful as cellular stains. In some aspect of the invention, the nuclear stains are useful for staining the nuclei of dead or fixed cells. Another aspect of the invention relates to substituted anthraquinone dyes comprising an enzyme substrate moiety that is transformable or cleavable by an enzyme such that the transformation or cleavage of the substrate moiety causes a detectable change in the functionality or spectral properties of the dye.
摘要翻译：本发明公开了可用作细胞污渍的新的取代的蒽醌染料。在本发明的某些方面，核染料可用于染色死细胞或固定细胞的细胞核。本发明的另一方面涉及包含酶底物部分的取代的蒽醌染料，其可由酶转化或切割，使得底物部分的转化或切割导致染料的官能度或光谱性质的可检测变化。

9. 发明授权

US08530195B2 Dyes and labeled molecules 有权
标题翻译：染料和标记分子
公开(公告)号：US08530195B2
公开(公告)日：2013-09-10
申请号：US12820983
申请日：2010-06-22
申请人： Fei Mao , Wai-Yee Leung , Xing Xin
发明人： Fei Mao , Wai-Yee Leung , Xing Xin
IPC分类号： C12P19/34 , C12Q1/68 , C07H21/04
CPC分类号： C12Q1/6876 , C07D209/08 , C07D213/38 , C07D221/10 , C07D417/06 , C07D417/14 , C07D491/147 , C07H21/00 , C09B15/00 , C12Q1/6851 , C12Q1/686 , C12Q2563/173
摘要： Dimeric and trimeric nucleic acid dyes, and associated systems and methods are provided. Such a dye may form a hairpin-like structure that enables it to stain nucleic acids via a release-on-demand mechanism, for example. Such a dye may have low background fluorescence in the absence of nucleic acids and high fluorescence in the presence of nucleic acids, upon binding therewith, for example. A dye provided herein may be useful in a variety of applications, such as in DNA quantitation in real-time PCR, for example.
摘要翻译：提供二聚和三聚体核酸染料以及相关系统和方法。这样的染料可以形成发夹状结构，使其能够通过例如按需释放机制来染色核酸。在不存在核酸的情况下，这样的染料可能具有低的背景荧光，并且例如在与核酸的结合时在核酸存在下具有高荧光。本文提供的染料可用于各种应用，例如在实时PCR中的DNA定量中。

10. 发明授权

US08232050B2 Methods of using dyes in association with nucleic acid staining or detection and associated technology 有权
标题翻译：使用染料与核酸染色或检测及相关技术相结合的方法
公开(公告)号：US08232050B2
公开(公告)日：2012-07-31
申请号：US12854436
申请日：2010-08-11
申请人： Fei Mao , Wai-Yee Leung
发明人： Fei Mao , Wai-Yee Leung
IPC分类号： C12Q1/68 , C07H21/00 , C07H19/04
CPC分类号： C09B15/00 , C09B11/28 , C09B23/04 , C12Q1/6818 , G01N1/30 , G01N21/6486 , Y10S435/81 , Y10T436/143333 , C12Q2525/301
摘要： Methods of using dyes and associated technology are provided. A dye, such as a monomeric dye or a dimeric dye, may be used in a nucleic acid gel staining application and/or a nucleic acid detection application. Such a dye and a salt that comprises an anion that is associated with a strong acid and a cation that is associated with a strong base may be used in such an application. A dimeric dye, such as a dimeric dye capable of forming a hairpin-like structure, may be used to stain and/or detect nucleic acids via a release-on-demand mechanism. A dimeric dye having low background fluorescence in the absence of nucleic acids and high fluorescence in the presence of nucleic acids, upon binding therewith, may be used to stain and/or detect nucleic acids.
摘要翻译：提供了使用染料和相关技术的方法。可以在核酸凝胶染色应用和/或核酸检测应用中使用染料，例如单体染料或二聚染料。这种染料和包含与强酸相关的阴离子和与强碱相关的阳离子的盐可用于这种应用中。可以使用二聚染料，例如能够形成发夹状结构的二聚染料，以通过按需释放机制对核酸进行染色和/或检测。在存在核酸时具有低背景荧光的二聚染料和在存在核酸时与核酸存在时的高荧光可用于染色和/或检测核酸。

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