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    • 9. 发明专利
    • METHOD FOR SEPARATING BILE ACID
    • JPS61260097A
    • 1986-11-18
    • JP10287085
    • 1985-05-15
    • HITACHI LTD
    • MATSUZAKI HARUMIKUBOTA MASAYOSHITAKAHASHI SANKICHIYOSHIDA MASAHIROTAKAKUSAKI TSUNEHIKOSATO JOSHIRO
    • C07J9/00
    • PURPOSE:To readily separate bile acid of high purity even in the case where a raw material with a low bile acid content is used, by separating the bile acid from an alcohol obtained by extracting an esterification solution of a raw material containing the bile acid with a supercritical gas or liquefied gas. CONSTITUTION:A raw material containing bile acid, e.g. fish oil, is esterified with a lower alcohol under conditions to form an excess alcohol, and the resultant esterification solution 10 is then introduced into an extraction tank 1 and brought into contact with, for example, a supercritical gaseous carbon dioxide 20 to dissolve preferentially a fatty acid ester, alcohol and bile acid ester in the supercritical gaseous carbon dioxide. The esterification solution 10 and supercritical gaseous carbon dioxide are separated by specific gravity difference to introduce the supercritical gaseous carbon dioxide 20' into the first-stage separation tank 2 and lower the density of the critical gaseous carbon dioxide. Thus, the fatty acid ester 12 is preferantially separated. The alcohol 11 and free bile acid ester 14 are then separated in the secon-stage separation tank 3, and the resultant bile acid ester is separated by crystallization.