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1. 发明专利

JP2004208547A Method for evaluating depression 审中-公开
标题翻译：评估渗漏的方法
公开(公告)号：JP2004208547A
公开(公告)日：2004-07-29
申请号：JP2002380614
申请日：2002-12-27
申请人： Hitachi Ltd , Tetsuo Omori , Kazuhito Rokutan , 一仁六反 , 哲郎大森 , 株式会社日立製作所
发明人： ROKUTAN KAZUHITO , OMORI TETSUO , SAITO TOSHIRO , TOMITA HIROYUKI , KATO KOICHI , NARAHARA MASATOSHI
IPC分类号： G01N33/53 , C12M1/00 , C12N15/09 , C12Q1/68 , G01N33/566
CPC分类号： C12Q1/6883 , C12Q1/6837 , C12Q2600/158
摘要： PROBLEM TO BE SOLVED: To provide a method for evaluating depression by measuring the expression amount of a specific gene group in the peripheral blood leukocyte of a testee, by which the depression can simply and accurately be evaluated.
SOLUTION: This method for evaluating the depression is characterized by analyzing the expression amount of at least one gene selected from apoptosis-related gene, ATPase-related gene, cell cycle-related gene, cytokine-related gene, heat shock protein-related gene, polymerase-related gene, GTP-bound protein-related gene, protein kinase C-related gene, and mitochondria cytochrome C oxidase-related gene from a messenger RNA originating from the peripheral blood leukocyte of the testee and then evaluating the depression state of the testee on the basis of the analysis result.
COPYRIGHT: (C)2004,JPO&NCIPI
摘要翻译：待解决的问题：提供一种通过测量受试者的外周血白细胞中特定基因组的表达量来评估抑郁症的方法，由此可以简单且准确地评价抑郁症。解决方案：该方法用于分析从凋亡相关基因，ATP酶相关基因，细胞周期相关基因，细胞因子相关基因，热休克蛋白相关基因中选出的至少一种基因的表达量，相关基因，聚合酶相关基因，GTP结合蛋白相关基因，蛋白激酶C相关基因和源自受试者外周血白细胞的信使RNA的线粒体细胞色素C氧化酶相关基因，然后评估抑郁状态的受试者在分析结果的基础上。版权所有（C）2004，JPO＆NCIPI

2. 发明专利

JP2007185140A Method for measuring slight amount of sample 审中-公开
标题翻译：测量样品量的方法
公开(公告)号：JP2007185140A
公开(公告)日：2007-07-26
申请号：JP2006006169
申请日：2006-01-13
申请人： Hitachi Ltd , 株式会社日立製作所
发明人： KATO KOICHI , SAITO TOSHIRO , NARAHARA MASATOSHI
IPC分类号： C12Q1/68 , C12N15/09 , G01N33/53 , G01N33/566 , G01N37/00
摘要： PROBLEM TO BE SOLVED: To reduce the amount of a sample required for one time measurement in a gene expression analysis by using a DNA chip for reduction of the cost and labor for the same.
SOLUTION: This method for performing the gene expression analysis of a sample containing ≤15 μg total RNA is performed by using a DNA-immobilized sample having ≤100 mm
2 area and used in the hybridization with the sample.
COPYRIGHT: (C)2007,JPO&INPIT
摘要翻译：要解决的问题：通过使用DNA芯片降低成本和劳动力来减少基因表达分析中一次性测量所需的样品量。解决方案：使用含有≤15μg总RNA的样品进行基因表达分析的方法是通过使用具有≤100mm 2 面积的DNA固定化样品进行的，并用于与例子。版权所有（C）2007，JPO＆INPIT

3. 发明专利

JP2003052385A Probe sequence determination system for dna array 审中-公开
标题翻译：用于DNA阵列的探针序列测定系统
公开(公告)号：JP2003052385A
公开(公告)日：2003-02-25
申请号：JP2002111490
申请日：2002-04-15
申请人： Hitachi Ltd , 株式会社日立製作所
发明人： TOMITA HIROYUKI , SAITO TOSHIRO , NARAHARA MASATOSHI , KATO KOICHI
IPC分类号： G01N33/48 , C12N15/09 , C12Q1/68
摘要： PROBLEM TO BE SOLVED: To realize a probe sequence without cross-hybridization, having an equalized Tm and slightly assuming a secondary structure in order to assure the assay accuracy and reproducibility as a probe sequence for a DNA array. SOLUTION: The probe sequence determination for the DNA array is carried out by passing the probe design flow through the following at least two steps. A step of preparing a probe candidate list for the DNA probe array composed of a partial sequence of the genome or a cDNA sequence (a listing step) and a step of excluding candidates having the melting temperature deviating from the predetermined range or high stability of the secondary structure or possibility of cross-hybridization (a filtering step). Thereby, a method for designing the sequence of the probe DNA immobilized on the DNA probe array is provided.
摘要翻译：要解决的问题：为了实现没有交叉杂交的探针序列，具有均衡的Tm并稍微假设二级结构，以确保作为DNA阵列的探针序列的测定精度和重现性。解决方案：通过将探针设计流程通过以下至少两个步骤来进行DNA阵列的探针序列测定。制备由基因组的部分序列或cDNA序列构成的DNA探针阵列的探针候选序列的步骤（列举步骤）和排除具有偏离预定范围或高稳定性的熔解温度的候选物的步骤二级结构或交叉杂交的可能性（过滤步骤）。因此，提供了设计固定在DNA探针阵列上的探针DNA序列的方法。

4. 发明专利

JP2006141210A Method for detecting chimera gene 审中-公开
标题翻译：检测CHIMERA基因的方法
公开(公告)号：JP2006141210A
公开(公告)日：2006-06-08
申请号：JP2004331808
申请日：2004-11-16
申请人： Hitachi High-Technologies Corp , Hitachi Ltd , 株式会社日立ハイテクノロジーズ , 株式会社日立製作所
发明人： NARAHARA MASATOSHI , SAITO TOSHIRO , OBARA TAKAYUKI , YASUDA KENJI
IPC分类号： C12Q1/68 , C12N15/09
CPC分类号： C12Q1/6806 , C12Q1/6827 , C12Q1/6834 , C12Q1/6886 , C12Q2600/158 , C12Q2525/143 , C12Q2521/119 , C12Q2525/149
摘要： PROBLEM TO BE SOLVED: To provide a method for detecting chimera genes produced from chromosomal translocations, with which a great number of existing chimera genes are detected at once in a high throughput.
SOLUTION: The method for detecting chimera genes comprises hybridizing a sample containing a nucleic acid derived from a chimera gene with at least two or more kinds of probes including a partial base sequence in an exon sequence across a translocation point of a chimera gene immobilized on a substrate in-between or a base sequence complementary thereto.
COPYRIGHT: (C)2006,JPO&NCIPI
摘要翻译：要解决的问题：提供一种用于检测由染色体易位产生的嵌合基因的方法，利用该方法在高通量中立即检测到大量现有嵌合基因。解决方案：检测嵌合基因的方法包括将含有源自嵌合基因的核酸的样品与包含嵌合基因的易位位置的外显子序列中的部分碱基序列的至少两种或更多种探针杂交固定在其上的底物或与其互补的碱基序列之间。版权所有（C）2006，JPO＆NCIPI

5. 发明专利

JP2004028926A Method for predicting effectiveness of interferon beta medicine treatment to multiple sclerosis 有权
公开(公告)号：JP2004028926A
公开(公告)日：2004-01-29
申请号：JP2002188932
申请日：2002-06-28
申请人： Hitachi Ltd , 株式会社日立製作所
发明人： YAMAMURA TAKASHI , SATO JUNICHI , SAITO TOSHIRO , TOMITA HIROYUKI , NARAHARA MASATOSHI , KATO KOICHI
IPC分类号： G01N33/53 , A61K38/21 , A61P25/00 , C12M1/00 , C12N15/09 , C12Q1/68 , G01N33/48 , G01N37/00
CPC分类号： C12Q1/6883 , C12Q1/6837 , C12Q2600/158
摘要： PROBLEM TO BE SOLVED: To provide a method for predicting the presence of medicinal efficacy simply and with high reliability without increasing a load on the patient when a patient of multiple sclerosis is treated by medicine due to interferon beta.
SOLUTION: A database which records the correlation between the presence of the medicinal efficacy of the interferon beta administration, and an interferon inducing protein, interferon regulatory factor and the expression level of the gene of a chemokine is used. The presence of the medicinal efficacy of the interferon administration is predicted by measuring the expression level of the gene thereof from mRNA derived from peripheral blood leukocyte of a subject.
COPYRIGHT: (C)2004,JPO

6. 发明专利

JP2004016070A Oligonucleotide array for measuring ortholog gene expression distribution 审中-公开
公开(公告)号：JP2004016070A
公开(公告)日：2004-01-22
申请号：JP2002174208
申请日：2002-06-14
申请人： Hitachi Ltd , 株式会社日立製作所
发明人： TOMITA HIROYUKI , SAITO TOSHIRO , NARAHARA MASATOSHI , KATO KOICHI
IPC分类号： G01N33/53 , C12M1/00 , C12M1/34 , C12N15/09 , C12Q1/68 , G01N37/00
摘要： PROBLEM TO BE SOLVED: To provide an oligonucleotide array which enables the extrapolation of data for predicting toxicity and medicinal actions in an experimental animal (for example, rat or mouse) to man with a DNA chip having excellent performance for predicting the toxicity and the medicinal actions, and to provide a method using the same.
SOLUTION: This array in which a plurality of oligonucleotides having different base sequences are immobilized at different positions on a support is characterized in that the partial sequences of the ortholog genes of species A and B are used as probes and cross hybridization is reduced in an extent which can substantially be ignored.
COPYRIGHT: (C)2004,JPO

7. 发明专利

JP2006194611A Comparison analysis method of in vivo polymer 审中-公开
标题翻译：生物聚合物的比较分析方法
公开(公告)号：JP2006194611A
公开(公告)日：2006-07-27
申请号：JP2005003832
申请日：2005-01-11
申请人： Hitachi Ltd , 株式会社日立製作所
发明人： NARAHARA MASATOSHI , SAITO TOSHIRO , KATO KOICHI , OTA MASAYUKI
IPC分类号： G01N33/50 , C12N15/09 , G01N33/68
摘要： PROBLEM TO BE SOLVED: To provide a method for specifying accurately with high reproducibility comparison data of in vivo polymers wherein in vivo polymers having large dispersion between samples exist in great numbers in the comparison data, in the case where the number of the comparison data of the in vivo polymers which are analysis objects is small.
SOLUTION: In comparison analysis wherein each abundance of in vivo polymers existing in two different kinds of samples is used as an index, a plurality of in vivo polymers wherein dispersion of the abundance between the samples is known to be small are selected, and a correction value of signal intensity showing the abundance is calculated, and the comparison data between the two samples are specified by using the correction value.
COPYRIGHT: (C)2006,JPO&NCIPI
摘要翻译：要解决的问题：为了提供一种精确地指定体内聚合物的高再现性比较数据的方法，其中在比较数据中存在大量样品间的分散体积较大的体内聚合物，作为分析对象的体内聚合物的比较数据较少。解决方案：选择使用存在于两种不同种类的样品中的各种丰度的体内聚合物作为指标的比较分析，选择其中已知样品之间丰度的分散小的多种体内聚合物，并计算显示丰度的信号强度的校正值，并且通过使用校正值来指定两个样本之间的比较数据。版权所有（C）2006，JPO＆NCIPI

8. 发明专利

JP2003339375A Hybridization method and apparatus 有权
标题翻译：混合方法和装置
公开(公告)号：JP2003339375A
公开(公告)日：2003-12-02
申请号：JP2002150446
申请日：2002-05-24
申请人： Hitachi Ltd , 株式会社日立製作所
发明人： KATO KOICHI , SAITO TOSHIRO , TOMITA HIROYUKI , NARAHARA MASATOSHI
IPC分类号： G01N33/53 , C12M1/00 , C12N15/09 , C12Q1/68 , G01N37/00
摘要： PROBLEM TO BE SOLVED: To provide a hybridization method and apparatus to improve the hybridization efficiency of a target DNA relative to the probe DNA immobilized on a substrate surface in a DNA chip assay.
SOLUTION: A hybridization solution containing fine particles is sealed on a DNA chip with a cover glass and a sealing agent. The hybridization chamber holding the DNA chip is rotated on a rotary shaft. Since the fine particles are moved in the direction of the gravitation force, these particles have an effect to stir the hybridization solution. The hybridization efficiency is increased by the increase of the diffusion distance of the DNA target in the hybridization solution. The method is effective for improving the hybridization efficiency in the DNA chip assay and attains a signal having high quantitative accuracy and reproducibility.
COPYRIGHT: (C)2004,JPO
摘要翻译：要解决的问题：提供在DNA芯片测定中提高靶DNA相对于固定在底物表面上的探针DNA的杂交效率的杂交方法和装置。解决方案：将含有细颗粒的杂交溶液用盖玻片和密封剂密封在DNA芯片上。保持DNA芯片的杂交室在旋转轴上旋转。由于微粒在重力方向上移动，所以这些粒子具有搅拌杂交溶液的作用。通过杂交溶液中DNA靶的扩散距离的增加，增加了杂交效率。该方法对于提高DNA芯片测定中的杂交效率是有效的，并获得具有高定量精度和重复性的信号。版权所有（C）2004，JPO

9. 发明专利

JP2004201589A Method for identifying bacterium 审中-公开
公开(公告)号：JP2004201589A
公开(公告)日：2004-07-22
申请号：JP2002375194
申请日：2002-12-25
申请人： Bml Inc , Hitachi Ltd , 株式会社ビー・エム・エル , 株式会社日立製作所
发明人： SAITO TOSHIRO , KATO KOICHI , NARAHARA MASATOSHI , TOMITA HIROYUKI , SHIMOJIMA MASAHIRO , ICHIMURA SADAHIRO , SUGIURA MASAHIKO
IPC分类号： G01N33/48 , C12N15/09 , C12Q1/68 , G01N33/53 , G01N33/566 , G01N33/58 , G01N37/00
摘要： PROBLEM TO BE SOLVED: To enable a bacterium to be readily identified and detected at a low cost in high reliability, especially to enable the mechanism of the action thereof to be examined.
SOLUTION: The method is carried out by forming a DNA microarray by immobilizing a genome DNA extracted from a specimen on a substrate, hybridizing the resultant microarray with a probe DNA having a base sequence peculiar to a well-known genome of the species of the bacterium. As a result, not only the species of the bacterium is accurately identified in a genome level, but also the cost is reduced and the time required for the detection is shortened by parallel assay. Two or more species are simultaneously identified and detected at once by hybridizing oligonucleotides labeled with different fluorochromes for each species with the array to extremely shorten the time required for the detection.
COPYRIGHT: (C)2004,JPO&NCIPI

10. 发明专利

JP2004041121A Method and system for analyzing allergy 审中-公开
公开(公告)号：JP2004041121A
公开(公告)日：2004-02-12
申请号：JP2002204785
申请日：2002-07-12
申请人： Hitachi Ltd , 株式会社日立製作所
发明人： TOMITA HIROYUKI , SAITO TOSHIRO , NARAHARA MASATOSHI , KATO KOICHI , YOSHIKAWA EMIKO
IPC分类号： G01N33/53 , C12N15/00 , C12Q1/02 , C12Q1/68 , G01N37/00
CPC分类号： C12Q1/6883 , C12Q2600/158 , G06F19/20
摘要： PROBLEM TO BE SOLVED: To provide a method for analyzing the presence of specific cells in a system with several kinds of cells without isolating the specific cells. SOLUTION: Directly using leukocyte-derived RNA extracted from peripheral blood, the assay value is corrected, thereby the balance(Th1/Th2) for helper T(Th) and the number ratio:Th1 cells/Th2 cells in a nucleic acid sample or the respective Th1/Th2 data for a patient and a healthy subject are compared with each other to analyze allergy. Further, by specifying a group of genes indispensable for examining the Th1/Th2, the necessary number of DNA fragments( oligonucleotides ) to be laid on an array is minimized, thereby the objective method and system for analyzing allergy using the array for this purpose are provided in high reproducibility and reliability without isolating specific cells. COPYRIGHT: (C)2004,JPO

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