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1. 发明授权

US07993875B2 β-galactoside-α2,6-sialyltransferase, a gene encoding thereof, and a method for producing thereof 失效
标题翻译： β-半乳糖苷-α2,6-唾液酸转移酶及其制备方法
公开(公告)号：US07993875B2
公开(公告)日：2011-08-09
申请号：US12225148
申请日：2006-08-10
申请人： Hiroshi Tsukamoto , Toshiki Mine , Yoshimitsu Takakura , Takeshi Yamamoto
发明人： Hiroshi Tsukamoto , Toshiki Mine , Yoshimitsu Takakura , Takeshi Yamamoto
IPC分类号： C12P21/02
CPC分类号： C12N9/1081 , C07H21/04
摘要： The present invention provides a novel β-galactoside-α2,6-sialyltransferase having high productivity and/or high activity, and a nucleic acid encoding the sialyltransferase. The present invention also provides a microorganism producing the sialyltransferase. The present invention further provides a vector carrying a nucleic acid encoding the sialyltransferase, and a host cell transformed with the vector, as well as a method for producing a recombinant β-galactoside-α2,6-sialyltransferase.
摘要翻译：本发明提供了具有高生产力和/或高活性的新颖的β-半乳糖苷-α2,6-唾液酸转移酶和编码唾液酸转移酶的核酸。本发明还提供了产生唾液酸转移酶的微生物。本发明还提供了携带编码唾液酸转移酶的核酸的载体和用载体转化的宿主细胞，以及用于产生重组和半乳糖苷-α2,6-唾液酸转移酶的方法。

2. 发明申请

US20100112616A1 NOVEL BETA-GALACTOSIDE-a2,6-SIALYLTRANSFERASE, A GENE ENCODING THEREOF, AND A METHOD FOR ENHANCING ENZYME ACTIVITY 失效
标题翻译：新型β-GALACTOSIDE-a2,6-SIALYLTRANSFERASE，其编码的基因，以及用于增强酶活性的方法
公开(公告)号：US20100112616A1
公开(公告)日：2010-05-06
申请号：US12529496
申请日：2008-03-03
申请人： Takeshi Yamamoto , Yoshimitsu Takakura , Toshiki Mine , Yoko Hamada , Hitomi Kajiwara , Masako Ichikawa , Hiroshi Tsukamoto
发明人： Takeshi Yamamoto , Yoshimitsu Takakura , Toshiki Mine , Yoko Hamada , Hitomi Kajiwara , Masako Ichikawa , Hiroshi Tsukamoto
IPC分类号： C12Q1/48 , C12P21/06 , C12N9/10 , C12N5/00 , C12N15/63 , C07H21/04
CPC分类号： C12N9/1081
摘要： The present invention provides an extremely useful and novel β-galactoside-α2,6-sialyltransferase having an optimum reaction pH in a neutral to alkaline range, and a nucleic acid encoding the sialyltransferase. The present invention further provides a vector carrying a nucleic acid encoding the sialyltransferase, and a host cell transformed with the vector, as well as a method for producing a recombinant β-galactoside-α2,6-sialyltransferase.
摘要翻译：本发明提供了在中性至碱性范围内具有最佳反应pH的非常有用和新颖的β-半乳糖苷-α2,6-唾液酸转移酶，以及编码唾液酸转移酶的核酸。本发明还提供了携带编码唾液酸转移酶的核酸的载体和用载体转化的宿主细胞，以及用于产生重组和半乳糖苷-α2,6-唾液酸转移酶的方法。

3. 发明申请

US20120282659A1 NOVEL BETA-GALACTOSIDE-ALPHA2,6-SIALYLTRANSFERASE, A GENE ENCODING THEREOF, AND A METHOD FOR ENHANCING ENZYME ACTIVITY 失效
标题翻译：新型β-GALACTOSIDE-ALPHA2,6-SIALYLTRANSFERASE，其编码的基因和用于增强酶活性的方法
公开(公告)号：US20120282659A1
公开(公告)日：2012-11-08
申请号：US13455770
申请日：2012-04-25
申请人： Takeshi Yamamoto , Yoshimitsu Takakura , Toshiki Mine , Yoko Hamada , Hitomi Kajiwara , Masako Ichikawa , Hiroshi Tsukamoto
发明人： Takeshi Yamamoto , Yoshimitsu Takakura , Toshiki Mine , Yoko Hamada , Hitomi Kajiwara , Masako Ichikawa , Hiroshi Tsukamoto
IPC分类号： C12N9/10 , C12N15/54 , C12N15/63 , C12P19/18 , C12N1/21
CPC分类号： C12N9/1081
摘要： The present invention provides an extremely useful and novel β-galactoside-α2,6-sialyltransferase having an optimum reaction pH in a neutral to alkaline range, and a nucleic acid encoding the sialyltransferase. The present invention further provides a vector carrying a nucleic acid encoding the sialyltransferase, and a host cell transformed with the vector, as well as a method for producing a recombinant β-galactoside-α2,6-sialyltransferase.
摘要翻译：本发明提供了在中性至碱性范围内具有最佳反应pH的非常有用和新颖的β-半乳糖苷-α2,6-唾液酸转移酶，以及编码唾液酸转移酶的核酸。本发明还提供了携带编码唾液酸转移酶的核酸的载体和用载体转化的宿主细胞，以及用于产生重组和半乳糖苷-α2,6-唾液酸转移酶的方法。

4. 发明授权

US08187853B2 β-galactoside-α2,6-sialyltransferase, a gene encoding thereof, and a method for enhancing enzyme activity 失效
标题翻译： β-半乳糖苷-α2,6-唾液酸转移酶，其编码基因，以及提高酶活性的方法
公开(公告)号：US08187853B2
公开(公告)日：2012-05-29
申请号：US12529496
申请日：2008-03-03
申请人： Takeshi Yamamoto , Yoshimitsu Takakura , Toshiki Mine , Yoko Hamada , Hitomi Kajiwara , Masako Ichikawa , Hiroshi Tsukamoto
发明人： Takeshi Yamamoto , Yoshimitsu Takakura , Toshiki Mine , Yoko Hamada , Hitomi Kajiwara , Masako Ichikawa , Hiroshi Tsukamoto
IPC分类号： C12N9/10
CPC分类号： C12N9/1081
摘要： The present invention provides an extremely useful and novel β-galactoside-α2,6-sialyltransferase having an optimum reaction pH in a neutral to alkaline range, and a nucleic acid encoding the sialyltransferase. The present invention further provides a vector carrying a nucleic acid encoding the sialyltransferase, and a host cell transformed with the vector, as well as a method for producing a recombinant β-galactoside-α2,6-sialyltransferase.
摘要翻译：本发明提供了在中性至碱性范围内具有最佳反应pH的非常有用和新颖的β-半乳糖苷-α2,6-唾液酸转移酶，以及编码唾液酸转移酶的核酸。本发明还提供了携带编码唾液酸转移酶的核酸的载体和用载体转化的宿主细胞，以及用于产生重组和半乳糖苷-α2,6-唾液酸转移酶的方法。

5. 发明申请

US20090291471A1 Novel Beta-Galactoside Alpha 2,6-Sialyltransferase, Gene Coding For The Transferase And Process For Producing The Same 失效
标题翻译：新型β-半乳糖苷α2,6-唾液酸转移酶，转移酶的基因编码及其生产方法
公开(公告)号：US20090291471A1
公开(公告)日：2009-11-26
申请号：US12225148
申请日：2006-08-10
申请人： Hiroshi Tsukamoto , Toshiki Mine , Yoshimitsu Takakura , Takeshi Yamamoto
发明人： Hiroshi Tsukamoto , Toshiki Mine , Yoshimitsu Takakura , Takeshi Yamamoto
IPC分类号： C12P21/02 , C07K14/00 , C12N9/10 , C07H21/00 , C12N15/74 , C12N1/20 , C07K16/00
CPC分类号： C12N9/1081 , C07H21/04
摘要： The present invention provides a novel β-galactoside-α2,6-sialyltransferase having high productivity and/or high activity, and a nucleic acid encoding the sialyltransferase. The present invention also provides a microorganism producing the sialyltransferase. The present invention further provides a vector carrying a nucleic acid encoding the sialyltransferase, and a host cell transformed with the vector, as well as a method for producing a recombinant β-galactoside-α2,6-sialyltransferase.
摘要翻译：本发明提供了具有高生产力和/或高活性的新型β-半乳糖苷-α2,6-唾液酸转移酶和编码唾液酸转移酶的核酸。本发明还提供了产生唾液酸转移酶的微生物。本发明还提供了携带编码唾液酸转移酶的核酸的载体和用该载体转化的宿主细胞，以及用于产生重组β-半乳糖苷-α2,6-唾液酸转移酶的方法。

6. 发明申请

US20100304460A1 NOVEL BETA-GALACTOSIDE-ALPHA2, 6-SIALYLTRANSFERASE, A GENE ENCODING THEREOF, AND A METHOD FOR PRODUCING THEREOF 失效
标题翻译：新型β-GALACTOSIDE-ALPHA2,6-SIALYLTRANSFERASE，其编码的基因及其生产方法
公开(公告)号：US20100304460A1
公开(公告)日：2010-12-02
申请号：US12789167
申请日：2010-05-27
申请人： Hiroshi TSUKAMOTO , Toshiki Mine , Yoshimitsu Takakura , Takeshi Yamamoto
发明人： Hiroshi TSUKAMOTO , Toshiki Mine , Yoshimitsu Takakura , Takeshi Yamamoto
IPC分类号： C12N9/10 , C07H21/04 , C12N15/63 , C12N1/21 , C12N1/20
CPC分类号： C12N9/1081 , C07H21/04
摘要： The present invention provides a novel β-galactoside-α2,6-sialyltransferase having high productivity and/or high activity, and a nucleic acid encoding the sialyltransferase. The present invention also provides a microorganism producing the sialyltransferase. The present invention further provides a vector carrying a nucleic acid encoding the sialyltransferase, and a host cell transformed with the vector, as well as a method for producing a recombinant β-galactoside-α2,6-sialyltransferase.
摘要翻译：本发明提供了具有高生产力和/或高活性的新颖的β-半乳糖苷-α2,6-唾液酸转移酶和编码唾液酸转移酶的核酸。本发明还提供了产生唾液酸转移酶的微生物。本发明还提供了携带编码唾液酸转移酶的核酸的载体和用载体转化的宿主细胞，以及用于产生重组和半乳糖苷-α2,6-唾液酸转移酶的方法。

7. 发明授权

US08372617B2 β-galactoside-α2,6-sialyltransferase, a gene encoding thereof, and a method for enhancing enzyme activity 失效
标题翻译： β-半乳糖苷-α2,6-唾液酸转移酶，其编码基因，以及提高酶活性的方法
公开(公告)号：US08372617B2
公开(公告)日：2013-02-12
申请号：US13455770
申请日：2012-04-25
申请人： Takeshi Yamamoto , Yoshimitsu Takakura , Toshiki Mine , Yoko Hamada , Hitomi Kajiwara , Masako Ichikawa , Hiroshi Tsukamoto
发明人： Takeshi Yamamoto , Yoshimitsu Takakura , Toshiki Mine , Yoko Hamada , Hitomi Kajiwara , Masako Ichikawa , Hiroshi Tsukamoto
IPC分类号： C12N9/10
CPC分类号： C12N9/1081
摘要： The present invention provides an extremely useful and novel β-galactoside-α2,6-sialyltransferase having an optimum reaction pH in a neutral to alkaline range, and a nucleic acid encoding the sialyltransferase. The present invention further provides a vector carrying a nucleic acid encoding the sialyltransferase, and a host cell transformed with the vector, as well as a method for producing a recombinant β-galactoside-α2,6-sialyltransferase.
摘要翻译：本发明提供了在中性至碱性范围内具有最佳反应pH的非常有用和新颖的β-半乳糖苷-α2,6-唾液酸转移酶，以及编码唾液酸转移酶的核酸。本发明还提供了携带编码唾液酸转移酶的核酸的载体和用载体转化的宿主细胞，以及用于产生重组和半乳糖苷-α2,6-唾液酸转移酶的方法。

8. 发明授权

US08187838B2 β-Galactoside-α2, 6-sialyltransferase, a gene encoding thereof, and a method for producing thereof 失效
标题翻译： - 糖苷-α2,6-唾液酸转移酶，其编码基因及其制备方法
公开(公告)号：US08187838B2
公开(公告)日：2012-05-29
申请号：US12789167
申请日：2010-05-27
申请人： Hiroshi Tsukamoto , Toshiki Mine , Yoshimitsu Takakura , Takeshi Yamamoto
发明人： Hiroshi Tsukamoto , Toshiki Mine , Yoshimitsu Takakura , Takeshi Yamamoto
IPC分类号： C12P21/02
CPC分类号： C12N9/1081 , C07H21/04
摘要： The present invention provides a novel β-galactoside-α2,6-sialyltransferase having high productivity and/or high activity, and a nucleic acid encoding the sialyltransferase. The present invention also provides a microorganism producing the sialyltransferase. The present invention further provides a vector carrying a nucleic acid encoding the sialyltransferase, and a host cell transformed with the vector, as well as a method for producing a recombinant β-galactoside-α2,6-sialyltransferase.
摘要翻译：本发明提供了具有高生产力和/或高活性的新颖的β-半乳糖苷-α2,6-唾液酸转移酶和编码唾液酸转移酶的核酸。本发明还提供了产生唾液酸转移酶的微生物。本发明还提供了携带编码唾液酸转移酶的核酸的载体和用载体转化的宿主细胞，以及用于产生重组和半乳糖苷-α2,6-唾液酸转移酶的方法。

9. 发明申请

US20120184016A1 NOVEL PROTEIN AND GENE THAT CODES THEREFOR 审中-公开
标题翻译：新蛋白及其代码
公开(公告)号：US20120184016A1
公开(公告)日：2012-07-19
申请号：US13377236
申请日：2010-06-11
申请人： Toshiki Mine , Takeshi Yamamoto , Hitomi Kajiwara , Hiroshi Tsukamoto
发明人： Toshiki Mine , Takeshi Yamamoto , Hitomi Kajiwara , Hiroshi Tsukamoto
IPC分类号： C12N9/10 , C12N15/56 , C12N15/54 , C12N5/10 , C07K16/40 , C12N1/21 , C12N1/19 , C12N9/24 , C12N15/63
CPC分类号： C12Y302/01018 , C12N9/1081 , C12N9/2402 , C12Y204/99001
摘要： The present invention provides a novel protein having neuraminidase activity and/or β-galactoside-α2,6-sialyltransferase activity and a nucleic acid encoding the protein. The present invention further provides a vector containing a nucleic acid encoding the protein, a host cell transformed with the vector, together with a method for producing a recombinant β-galactoside-α2,6-sialyltransferase. The present invention also provides an antibody specifically recognizing the protein.
摘要翻译：本发明提供了具有神经氨酸酶活性和/或β-半乳糖苷-α2,6-唾液酸转移酶活性的新蛋白质和编码该蛋白质的核酸。本发明还提供了含有编码该蛋白质的核酸，载体转化的宿主细胞以及重组α-半乳糖苷-α2,6-唾液酸转移酶的制备方法的载体。本发明还提供了特异性识别蛋白质的抗体。

10. 发明申请

US20100132068A1 Cosmid Vector For Plant Transformation And Use Thereof 有权
标题翻译：用于植物转化和使用的粘粒载体
公开(公告)号：US20100132068A1
公开(公告)日：2010-05-27
申请号：US12306163
申请日：2007-06-25
申请人： Yoshimitsu Takakura , Toshihiko Komari , Yuji Ishida , Toshiyuki Komori , Yukoh Hiei , Toshiki Mine , Teruyuki Imayama
发明人： Yoshimitsu Takakura , Toshihiko Komari , Yuji Ishida , Toshiyuki Komori , Yukoh Hiei , Toshiki Mine , Teruyuki Imayama
IPC分类号： A01H1/00 , C12N15/63
CPC分类号： C12N15/82 , C12N15/8205
摘要： The present invention aims to provide novel vectors for plant transformation.The vectors of the present invention are cosmid vectors having a full length of 15 kb or less characterized in that: 1) they contain an origin of replication of an IncP plasmid, but do not contain any origin of replication of other plasmid groups; 2) they contain the trfA1 gene of an IncP plasmid; 3) they contain an oriT of an IncP plasmid; 4) they contain the incC1 gene of an IncP plasmid; 5) they contain a cos site of lambda phage and the cos site is located outside the T-DNA; 6) they contain a drug resistance gene expressed in E. coli and a bacterium of the genus Agrobacterium; 7) they contain a T-DNA right border sequence of a bacterium of the genus Agrobacterium; 8) they contain a T-DNA left border sequence of a bacterium of the genus Agrobacterium; 9) they contain a selectable marker gene for plant transformation located between 7) and 8) and expressed in a plant; and 10) they contain restriction endonuclease recognition site(s) located between 7) and 8) for cloning a foreign gene.
摘要翻译：本发明旨在提供用于植物转化的新型载体。本发明的载体是全长为15kb或更小的粘粒载体，其特征在于：1）它们含有IncP质粒的复制起点，但不含有其他质粒组的任何复制起点; 2）它们含有IncP质粒的trfA1基因; 3）它们含有一个IncP质粒的oriT; 4）它们含有IncP质粒的incC1基因; 5）它们含有λ噬菌体的cos位点，cos位点位于T-DNA外部; 6）它们含有在大肠杆菌中表达的耐药基因和农杆菌属的细菌; 7）它们含有农杆菌属细菌的T-DNA右侧序列; 8）它们含有农杆菌属细菌的T-DNA左边界序列; 9）它们含有位于7）和8之间的植物转化的选择性标记基因并在植物中表达; 和10）它们含有位于7）和8之间的限制性内切核酸酶识别位点，用于克隆外来基因。

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