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1. 发明授权

US07192764B2 Cell surface layer-binding protein and utilization thereof 有权
标题翻译：细胞表面层结合蛋白及其利用
公开(公告)号：US07192764B2
公开(公告)日：2007-03-20
申请号：US10474431
申请日：2002-03-22
申请人： Hideki Fukuda , Akihiko Kondo , Takeshi Matsumoto , Hideo Noda
发明人： Hideki Fukuda , Akihiko Kondo , Takeshi Matsumoto , Hideo Noda
IPC分类号： C12N1/19 , C12N9/00 , C12N15/00 , C07K14/39 , C07H21/04
CPC分类号： C40B40/02 , C07K14/395 , C07K2319/00 , C07K2319/02 , C12N11/16 , C12N15/1037
摘要： A plasmid is constructed so as to express a fused protein of a sugar chain-binding protein domain with a desired protein. Then this plasmid is transferred into cells and thus the protein is expressed in the cell surface layer. This method is particularly adequate in case of expressing a protein having an activity in the C-terminal portion in cell surface layer.
摘要翻译：构建质粒以便表达具有所需蛋白质的糖链结合蛋白结构域的融合蛋白。然后将该质粒转移到细胞中，因此蛋白质在细胞表面层中表达。在表达在细胞表面层的C末端部分具有活性的蛋白质的情况下，该方法特别合适。

2. 发明授权

US08252577B2 Microorganism that displays biotin on cell surface 有权
标题翻译：在细胞表面显示生物素的微生物
公开(公告)号：US08252577B2
公开(公告)日：2012-08-28
申请号：US12431102
申请日：2009-04-28
申请人： Akihiko Kondo , Hideki Fukuda , Tsutomu Tanaka , Hideo Noda
发明人： Akihiko Kondo , Hideki Fukuda , Tsutomu Tanaka , Hideo Noda
IPC分类号： C12N1/20 , C12N1/00 , C12N9/10 , C12Q1/00 , C12Q1/48 , G01N33/567 , G01N33/53 , C12P21/06 , C12P21/04
CPC分类号： C12N15/1037 , C12N9/93
摘要： Provided is a microorganism that can display, on the cell surface, any molecules other than a molecule comprising amino acids, more specifically, a microorganism that displays biotin on a cell surface. The microorganism is capable of co-expressing a biotinylating enzyme and an acceptor peptide having a sequence recognized by the biotinylating enzyme, wherein the acceptor peptide is expressed on the cell surface, so that lysine of the acceptor peptide is biotinylated to display biotin on the cell surface. Also provided is a method for displaying an intended molecule, including not only a molecule comprising amino acids but also any molecules, on a cell surface of a microorganism.
摘要翻译：本发明提供能够在细胞表面上显示除氨基酸分子以外的任何分子，更具体地，在细胞表面上显示生物素的微生物的微生物。微生物能够共表达生物素化酶和具有生物素化酶识别的序列的受体肽，其中受体肽在细胞表面上表达，使得受体肽的赖氨酸被生物素化以在细胞上显示生物素表面。还提供了在微生物的细胞表面上显示预期分子的方法，不仅包括包含氨基酸的分子，而且包括任何分子。

3. 发明申请

US20090275110A1 Microorganism that Displays Biotin on Cell Surface 有权
标题翻译：在细胞表面显示生物素的微生物
公开(公告)号：US20090275110A1
公开(公告)日：2009-11-05
申请号：US12431102
申请日：2009-04-28
申请人： Akihiko Kondo , Hideki Fukuda , Tsutomu Tanaka , Hideo Noda
发明人： Akihiko Kondo , Hideki Fukuda , Tsutomu Tanaka , Hideo Noda
IPC分类号： C12N1/20 , C12N1/16
CPC分类号： C12N15/1037 , C12N9/93
摘要： Provided is a microorganism that can display, on the cell surface, any molecules other than a molecule comprising amino acids, more specifically, a microorganism that displays biotin on a cell surface. The microorganism is capable of co-expressing a biotinylating enzyme and an acceptor peptide having a sequence recognized by the biotinylating enzyme, wherein the acceptor peptide is expressed on the cell surface, so that lysine of the acceptor peptide is biotinylated to display biotin on the cell surface. Also provided is a method for displaying an intended molecule, including not only a molecule comprising amino acids but also any molecules, on a cell surface of a microorganism.
摘要翻译：本发明提供能够在细胞表面上显示除氨基酸分子以外的任何分子，更具体地，在细胞表面上显示生物素的微生物的微生物。微生物能够共表达生物素化酶和具有由生物素化酶识别的序列的受体肽，其中受体肽在细胞表面上表达，使得受体肽的赖氨酸被生物素化以在细胞上显示生物素表面。还提供了在微生物的细胞表面上显示预期分子的方法，不仅包括包含氨基酸的分子，而且包括任何分子。

4. 发明授权

US06982155B1 Process for producing fatty acid lower alcohol ester 失效
标题翻译：脂肪酸低级醇酯的制备方法
公开(公告)号：US06982155B1
公开(公告)日：2006-01-03
申请号：US10130977
申请日：2000-11-20
申请人： Hideki Fukuda , Hideo Noda
发明人： Hideki Fukuda , Hideo Noda
IPC分类号： C12P7/62
CPC分类号： C12P7/649 , C10G2300/1011 , C12P7/62 , Y02E50/13 , Y02P30/20
摘要： The present invention provides a method for producing a fatty acid ester inexpensively and efficiently by reacting a lipase or an intact mircroorganism containing a lipase, an oil and fat, and a linear lower alcohol in a system containing little or no solvent in the presence of water. The intact microorganism has not been treated with a solvent or the like, and is used as such, so that a fatty acid ester can be prepared in a simple manner. An oil and fat, a natural oil and fat such as a vegetable oil and fat, an animal oil and fat, as well as a waste oil thereof can be used. According to the method of the present invention, a reaction proceeds even in the presence of water, so that waste oils containing a large amount of water can be used as a raw material. Therefore, the waste oil to be dumped into the environment can be recycled, and a biodiesel fuel, which hardly pollutes the environment, can be provided at the same time.
摘要翻译：本发明提供了一种通过使含有脂肪酶，油脂和线型低级醇的完整的微生物在含有少量或不溶剂的体系中，在水存在下使脂肪酶或完整的微生物廉价且有效地生产脂肪酸酯的方法。。完整的微生物没有用溶剂等处理，因此可以这样使用，从而可以以简单的方式制备脂肪酸酯。可以使用油脂，天然油脂，如植物油脂，动物油和脂肪，以及废油。根据本发明的方法，即使在水的存在下也进行反应，因此可以使用含有大量水的废油作为原料。因此，可以将排入环境的废油再循环，同时可以提供几乎不污染环境的生物柴油。

5. 发明申请

US20100261235A1 CONTINUOUS PRODUCTION OF BIODIESEL FUEL BY ENZYMATIC METHOD 审中-公开
标题翻译：通过酶联免疫法连续生产生物柴油
公开(公告)号：US20100261235A1
公开(公告)日：2010-10-14
申请号：US12745962
申请日：2008-11-28
申请人： Nobuyuki Kuratani , Shinji Hama , Hideo Noda , Hideki Fukuda
发明人： Nobuyuki Kuratani , Shinji Hama , Hideo Noda , Hideki Fukuda
IPC分类号： C12P7/64 , C12M1/02
CPC分类号： C12P7/649 , C10G2300/1011 , C11C3/003 , C12P7/62 , Y02E50/13 , Y02P30/20
摘要： A method for continuously producing a fatty acid ester of the present invention comprises (a) mixing and agitating an oil and fat starting material and a lower alcohol, and supplying a mixture to one of the catalyst reaction tubes filled with a lipase; (b) producing a fatty acid ester and glycerin in the catalyst reaction tube; (c) introducing an outflowing liquid from the catalyst reaction tube into a glycerin separation tank, thereby collecting the glycerin; (d) adding a lower alcohol to a separated liquid obtained by separating the glycerin from the outflowing liquid, mixing and agitating an obtained material, and supplying a mixture to a following catalyst reaction tube; (e) repeating the steps (b) to (d) until supply to a last catalyst reaction tube is performed; and (f) collecting a fatty acid ester from the separated liquid obtained from the last catalyst reaction tube. According to the method of the present invention, the concentration of a lower alcohol can be strictly controlled and by product glycerin can be automatically removed.
摘要翻译：本发明的连续生产脂肪酸酯的方法包括：（a）将油脂原料和低级醇混合搅拌，向填充有脂肪酶的催化剂反应管之一供给混合物; （b）在催化剂反应管中制备脂肪酸酯和甘油; （c）将来自催化剂反应管的流出液引入甘油分离槽中，从而收集甘油; （d）向从流出液中分离甘油得到的分离液中加入低级醇，混合搅拌所得物质，向后述催化剂反应管供给混合物; （e）重复步骤（b）至（d），直到进行最后一个催化剂反应管的供应; 和（f）从最后一个催化剂反应管获得的分离液中收集脂肪酸酯。根据本发明的方法，可以严格控制低级醇的浓度，并且可以自动除去产物甘油。

6. 发明授权

US06524839B1 Method for enhancing catalytic activity of cells 失效
标题翻译：增强细胞催化活性的方法
公开(公告)号：US06524839B1
公开(公告)日：2003-02-25
申请号：US09744068
申请日：2001-03-12
申请人： Hideki Fukuda , Akihiko Kondo
发明人： Hideki Fukuda , Akihiko Kondo
IPC分类号： C12N138
CPC分类号： C12N1/32 , C12N1/38
摘要： Treatment of cells with lower alcohol provides cells having the reaction rate 350 to 600 times higher than that of cells that are not treated with lower alcohol More specifically, a simple operation of treating cells with lower alcohol provides a catalyst that has a higher activity that that of a cell extract and can be recycled.
摘要翻译：用低级醇处理细胞提供的细胞的反应速率比未用低级醇处理的细胞的反应速率高350至600倍更具体地说，用低级醇处理细胞的简单操作提供了具有较高活性的催化剂，的细胞提取物，可以回收利用。

7. 发明授权

US08441519B2 Optical disk for high resolution and three-dimensional video recording, optical disk reproduction apparatus and optical disk recording apparatus 有权
标题翻译：用于高分辨率和三维视频记录的光盘，光盘再现装置和光盘记录装置
公开(公告)号：US08441519B2
公开(公告)日：2013-05-14
申请号：US12683677
申请日：2010-01-07
申请人： Mitsuaki Oshima , Hiromu Kitaura , Hideki Fukuda , Hideshi Ishihara , Toshiyuki Kawara
发明人： Mitsuaki Oshima , Hiromu Kitaura , Hideki Fukuda , Hideshi Ishihara , Toshiyuki Kawara
IPC分类号： H04N15/00 , H04N13/02
CPC分类号： H04N13/172 , G11B20/00007 , G11B20/10222 , G11B20/10527 , G11B20/1217 , G11B20/14 , G11B27/005 , G11B27/10 , G11B27/105 , G11B2020/10546 , G11B2020/10675 , G11B2020/1074 , G11B2020/10805 , G11B2020/10953 , G11B2020/1288 , G11B2220/2562 , H04N5/04 , H04N5/4401 , H04N5/45 , H04N5/46 , H04N5/765 , H04N5/775 , H04N5/85 , H04N7/012 , H04N7/0122 , H04N9/7921 , H04N9/8042 , H04N9/8045 , H04N9/8063 , H04N9/8205 , H04N9/8227 , H04N9/877 , H04N13/10 , H04N13/144 , H04N13/15 , H04N13/161 , H04N13/167 , H04N13/178 , H04N13/183 , H04N13/189 , H04N13/194 , H04N13/239 , H04N13/341 , H04N13/359 , H04N13/361 , H04N13/363 , H04N13/398 , H04N19/597 , H04N19/61 , H04N21/42646 , H04N21/4305 , H04N21/4307 , H04N21/4325 , H04N21/4334 , H04N21/4392 , H04N2013/0085
摘要： The present invention has an objective of providing an optical disk having a high resolution picture and a system for reproducing data on the optical disk, which are compatible with a conventional system for reproducing an ordinary resolution picture. A high resolution signal is divided into a main signal and a sub signal by picture division means and MPEG-encoded. The main signal and the sub signal are divided into frames each having 1 GOP or more. The resultant first interleave block 54 and second interleave block 55 are recorded alternately on an optical disk. A high resolution reproduction apparatus reproduced both the first and second interleave blocks, so that a high resolution picture is obtained. A non-high resolution reproduction apparatus reproduces only the first or second interleave block, so that an ordinary resolution picture.
摘要翻译：本发明的目的是提供一种具有高分辨率图像的光盘和用于在光盘上再现数据的系统，其与用于再现普通分辨率图像的常规系统兼容。高分辨率信号由图像分割装置分割为主信号和副信号，并进行MPEG编码。主信号和子信号被分成具有1个GOP或更多GOP的帧。所得到的第一交错块54和第二交错块55交替地记录在光盘上。高分辨率再现装置再现第一和第二交错块，从而获得高分辨率图像。非高分辨率再现设备仅再现第一或第二交错块，从而使普通分辨率图像。

8. 发明授权

US08107794B2 Optical disk for high resolution and general video recording, optical disk reproduction apparatus, optical disk recording apparatus, and reproduction control information generation apparatus 有权
公开(公告)号：US08107794B2
公开(公告)日：2012-01-31
申请号：US12464977
申请日：2009-05-13
申请人： Mitsuaki Oshima , Hiromu Kitaura , Hideki Fukuda , Hideshi Ishihara
发明人： Mitsuaki Oshima , Hiromu Kitaura , Hideki Fukuda , Hideshi Ishihara
IPC分类号： H04N5/76 , H04N5/93
CPC分类号： H04N5/85 , G11B2020/10953 , H04N5/04 , H04N5/4401 , H04N5/45 , H04N5/46 , H04N5/765 , H04N5/775 , H04N7/012 , H04N7/0122 , H04N9/7921 , H04N9/8042 , H04N9/8063 , H04N9/8205 , H04N9/8227 , H04N9/877 , H04N13/10 , H04N13/15 , H04N13/161 , H04N13/167 , H04N13/178 , H04N13/189 , H04N13/194 , H04N13/239 , H04N13/341 , H04N13/361 , H04N13/363 , H04N13/398 , H04N19/597 , H04N21/42646 , H04N21/4307 , H04N21/4325 , H04N2013/0085
摘要： The present invention has an objective of realizing compatibility of an optical disk having a high resolution video signal recorded thereon and a system for reproducing the optical disk, with a conventional system for producing a standard resolution video signal. A high resolution video signal is divided by video division means into a main signal and a sub signal, and the main signal and the sub signal are MPEG-encoded. The stream of the main signal and the stream of the sub signal are divided into 1 GOP or more of frames. First interleave blocks 54 each including 1 GOP or more of the stream of the main signal and second interleave blocks 55 each including 1 GOP or more of the stream of the sub signal are recorded on an optical disk 1. A high resolution reproduction apparatus reproduces both the first and second interleave blocks to obtain a high resolution video output. A non-high quality picture reproduction apparatus reproduces only the first or second interleave blocks to obtain a standard resolution video output.

9. 发明申请

US20100247695A1 LATERALLY INDEPENDENTLY OPERATED COLUMN-EQUIPPED VULCANIZER 有权
标题翻译：独立操作的柱式VULCANIZER
公开(公告)号：US20100247695A1
公开(公告)日：2010-09-30
申请号：US12514346
申请日：2008-09-16
申请人： Katsuhide Aikawa , Hideki Fukuda , Hideaki Katayama
发明人： Katsuhide Aikawa , Hideki Fukuda , Hideaki Katayama
IPC分类号： B29C35/02
CPC分类号： B29D30/0601
摘要： A laterally independently operated column-equipped vulcanizer comprises two (right and left) die opening and closing devices (12A, 12B) disposed to be operable independently of each other, the die opening and closing devices having upper die mounting members (17a, 17b) corresponding to lower die mounting members (13a, 13b) installed on a base plate (11), the upper die mounting members being supported in an ascendable and descendable manner on right and left paired columns (18a, 18b) erected on the base plate. The right and left paired columns throughout the two die opening and closing devices are arranged in V-configuration as viewed in plan. Loaders (22a, 22b) capable of carrying green tires GT into the die opening and closing devices are disposed on the side of the columns narrowly spaced from each other in the lateral direction among the columns arranged in V-configuration. Unloaders (23a, 23b) capable of carrying vulcanized tires T out of the die opening and closing devices are disposed on the side of the columns widely spaced from each other in the lateral direction among the columns arranged in V-configuration. Thus, the entire vulcanizer can be rendered even more compact.
摘要翻译：横向独立操作的装有柱的硫化机包括两个（右和左）模具开闭装置（12A，12B），其设置成可彼此独立地操作，模具打开和关闭装置具有上模具安装构件（17a，17b）对应于安装在基板（11）上的下模安装构件（13a，13b），上模具安装构件以竖起在基板上的左右成对的列（18a，18b）上升和下降地支撑。两个模具打开和关闭装置中的左右对齐的列以计划中所示的V形配置。能够将绿色轮胎GT装载到模具打开和关闭装置中的装载机（22a，22b）设置在排列成V形构造的列之间沿横向彼此狭窄间隔的列的侧面。能够将硫化轮胎T从模具打开和关闭装置运出的卸载器（23a，23b）设置在排列成V形构造的列之间沿横向彼此宽间隔的列的一侧。因此，整个硫化机可以变得更加紧凑。

10. 发明申请

US20090220215A1 OPTICAL DISK FOR HIGH RESOLUTION AND GENERAL VIDEO RECORDING, OPTICAL DISK REPRODUCTION APPARATUS, OPTICAL DISK RECORDING APPARATUS, AND REPRODUCTION CONTROL INFORMATION GENERATION APPARATUS 有权
公开(公告)号：US20090220215A1
公开(公告)日：2009-09-03
申请号：US12464986
申请日：2009-05-13
申请人： Mitsuaki OSHIMA , Hiromu Kitaura , Hideki Fukuda , Hideshi Ishihara
发明人： Mitsuaki OSHIMA , Hiromu Kitaura , Hideki Fukuda , Hideshi Ishihara
IPC分类号： H04N7/00 , H04N5/00
CPC分类号： H04N5/85 , G11B2020/10953 , H04N5/04 , H04N5/4401 , H04N5/45 , H04N5/46 , H04N5/765 , H04N5/775 , H04N7/012 , H04N7/0122 , H04N9/7921 , H04N9/8042 , H04N9/8063 , H04N9/8205 , H04N9/8227 , H04N9/877 , H04N13/10 , H04N13/15 , H04N13/161 , H04N13/167 , H04N13/178 , H04N13/189 , H04N13/194 , H04N13/239 , H04N13/341 , H04N13/361 , H04N13/363 , H04N13/398 , H04N19/597 , H04N21/42646 , H04N21/4307 , H04N21/4325 , H04N2013/0085
摘要： The present invention has an objective of realizing compatibility of an optical disk having a high resolution video signal recorded thereon and a system for reproducing the optical disk, with a conventional system for producing a standard resolution video signal. A high resolution video signal is divided by video division means into a main signal and a sub signal, and the main signal and the sub signal are MPEG-encoded. The stream of the main signal and the stream of the sub signal are divided into 1 GOP or more of frames. First interleave blocks 54 each including 1 GOP or more of the stream of the main signal and second interleave blocks 55 each including 1 GOP or more of the stream of the sub signal are recorded on an optical disk 1. A high resolution reproduction apparatus reproduces both the first and second interleave blocks to obtain a high resolution video output. A non-high quality picture reproduction apparatus reproduces only the first or second interleave blocks to obtain a standard resolution video output.

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