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1. 发明授权

US08535909B2 E. coli BL21 strain lacking a functional group II capsular gene cluster 有权
标题翻译：缺乏功能性II型荚膜基因簇的大肠杆菌BL21菌株
公开(公告)号：US08535909B2
公开(公告)日：2013-09-17
申请号：US13126379
申请日：2009-11-10
申请人： Helle Fabricius Wöldike , Jianhui Deng , Xin Zhao , Yun Liu , Jing Su
发明人： Helle Fabricius Wöldike , Jianhui Deng , Xin Zhao , Yun Liu , Jing Su
IPC分类号： C12P21/06 , C12N1/20
CPC分类号： C12P21/02 , C07K14/245 , C07K14/61 , C12N1/20 , C12N15/70 , C12R1/19
摘要： The present invention relates to a novel non-pathogenic Escherichia coli (E. coli) B BL21 strain comprising a deletion of group II capsular gene cluster, and the use thereof for the production of peptides.
摘要翻译：本发明涉及一种包含II型荚膜基因簇缺失的新型非致病性大肠杆菌（E.coli）B BL21菌株及其生产肽的用途。

2. 发明申请

US20110236929A1 E. COLI BL21 STRAIN LACKING A FUNCTIONAL GROUP II CAPSULAR GENE CLUSTER 有权
标题翻译：大肠杆菌BL21菌株缺失功能组II基因簇
公开(公告)号：US20110236929A1
公开(公告)日：2011-09-29
申请号：US13126379
申请日：2009-11-10
申请人： Helle Fabricius Wöldike , Jianhui Deng , Xin Zhao , Yun Liu , Jing Su
发明人： Helle Fabricius Wöldike , Jianhui Deng , Xin Zhao , Yun Liu , Jing Su
IPC分类号： C12P21/00 , C12N1/20 , C12N1/21
CPC分类号： C12P21/02 , C07K14/245 , C07K14/61 , C12N1/20 , C12N15/70 , C12R1/19
摘要： The present invention relates to a novel non-pathogenic Escherichia coli (E. coli) B BL21 strain comprising a deletion of group II capsular gene cluster, and the use thereof for the production of peptides.
摘要翻译：本发明涉及一种包含II型荚膜基因簇缺失的新型非致病性大肠杆菌（E.coli）B BL21菌株及其生产肽的用途。

3. 发明授权

US07858747B2 Expression of proteins in E.coli 有权
标题翻译：蛋白质在大肠杆菌中的表达
公开(公告)号：US07858747B2
公开(公告)日：2010-12-28
申请号：US12064837
申请日：2006-08-30
申请人： Helle Fabricius Wöldike , Christine Bruun Schiødt
发明人： Helle Fabricius Wöldike , Christine Bruun Schiødt
IPC分类号： C07K1/00
CPC分类号： C07K14/54 , C07K2319/00 , C12P21/02
摘要： Plasmid comprising a DNA tag encoding a peptide tag of the sequence MX1(X2X3)n wherein X1 represents K, R or H; X2 represents A, G, H, M, S or T; X3 represents K, R, H, A, Q or V; n represents an integer of 1 or larger; and wherein said DNA is operably-linked to a promoter sequence are provided.
摘要翻译：质粒包含编码序列MX1（X2X3）n的肽标签的DNA标签，其中X1表示K，R或H; X2表示A，G，H，M，S或T; X3表示K，R，H，A，Q或V; n表示1以上的整数，并且其中所述DNA与启动子序列可操作地连接。

4. 发明授权

US07517668B1 Process for the production of protein products in aspergillus 失效
标题翻译：在曲霉菌中生产蛋白质产物的方法
公开(公告)号：US07517668B1
公开(公告)日：2009-04-14
申请号：US08435557
申请日：1995-05-05
申请人： Esper Boel , Tove Christensen , Helle Fabricius Wöldike
发明人： Esper Boel , Tove Christensen , Helle Fabricius Wöldike
IPC分类号： C12P21/06 , C12N15/00 , C12N15/74 , C12N9/30 , C07H21/04
CPC分类号： C12N15/80 , C07K2319/02 , C12N9/20 , C12N15/67
摘要： A process for expression of a protein product in Aspergillus oryzae is disclosed. The process comprises transforming Aspergillus oryzae with a vector system comprising DNA-sequences encoding functions facilitating gene expression, a suitable marker for selection of transformants, and a DNA-sequence encoding the desired protein product. The process enables industrial production of many different polypeptides and proteins in A. oryzae. Examples of such products are chymosin or prochymosin other rennets, proteases, lipases and amylases. Also disclosed is an effective promoter for expression of a protein in Aspergillus. A preferred promoter is the TAKA-amylase promoter or functional parts thereof. There is also provided a process for the production of a recombinant Humicola lipase. The recombinant Humicola lipase from A. oryzae differs from the native lipase in having a greater glycosylation and in exhibiting an improved thermostability.
摘要翻译：公开了在米曲霉中表达蛋白质产物的方法。该方法包括用包含编码促进基因表达的功能的DNA序列的载体系统，选择转化体的合适标记物和编码所需蛋白质产物的DNA序列来转化米曲霉。该方法能够在米曲霉中工业生产许多不同的多肽和蛋白质。这些产品的实例是凝乳酶或凝乳酶原等其他抗体，蛋白酶，脂肪酶和淀粉酶。还公开了用于在曲霉中表达蛋白质的有效启动子。优选的启动子是TAKA-淀粉酶启动子或其功能部分。还提供了用于生产重组Humicola脂肪酶的方法。来自米曲霉的重组Humicola脂肪酶与天然脂肪酶的不同之处在于具有更大的糖基化并具有改善的热稳定性。

5. 发明授权

US06171823B2 Process for producing extracellular proteins in bacteria 失效
标题翻译：在细菌中产生胞外蛋白的方法
公开(公告)号：US06171823B2
公开(公告)日：2001-01-09
申请号：US08849602
申请日：1997-06-02
申请人： Helle Fabricius Wöldike , Sven Hastrup
发明人： Helle Fabricius Wöldike , Sven Hastrup
IPC分类号： C12N1509
CPC分类号： C12N9/54 , C07K14/605 , C07K2319/00 , C07K2319/02 , C07K2319/75 , C12N9/20 , C12N9/52 , C12N15/62 , Y10S435/824
摘要： The invention relates to a method of producing an extracellular protein in a bacterium provided with an inner and an outer cell membrane, the method comprising: (a) providing a recombinant vector including a DNA construct comprising a DNA sequence encoding the prepropeptide or part of the prepropeptide of a bacterial extracellular protease selected from the group consisting of Achromobacter lyticus protease I, Bacillus metalloproteases and Bacillus serine proteases preceding and operably connected to a DNA sequence encoding a desired protein, (b) transforming cells of a microorganism provided with an inner and outer cell membrane with the recombinant vector of step (a), (c) culturing the transformed cells of step (b) under conditions permitting expression of said DNA insert and leakage of the bacterial extracellular protease propeptide fused to the desired protein into the culture medium, and (d) recovering the resulting protein from the medium.
摘要翻译：本发明涉及在具有内细胞膜和外细胞膜的细菌中产生细胞外蛋白质的方法，所述方法包括：（a）提供包含DNA构建体的重组载体，所述DNA构建体包含编码前肽的DNA序列或部分选自下组的细菌细胞外蛋白酶的前肽，所述细菌细胞外蛋白酶选自无菌杆菌蛋白酶I，芽孢杆菌金属蛋白酶和芽孢杆菌丝氨酸蛋白酶，之前和可操作地连接到编码所需蛋白质的DNA序列，（b）转化具有内部和外部的微生物的细胞细胞膜与步骤（a）的重组载体，（c）在允许所述DNA插入片段表达的条件下培养步骤（b）的转化细胞，并将融合到所需蛋白质的细菌细胞外蛋白酶前体的渗漏渗入培养基中，和（d）从培养基中回收得到的蛋白质。

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