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    • 3. 发明申请
    • LIPID-FREE SCAFFOLDS FOR HUMAN VOLUME REPLACEMENT OR CELL CULTURE AND USE THEREOF
    • 用于人体容量更换或细胞培养的无脂质免疫球蛋白及其用途
    • US20100178681A1
    • 2010-07-15
    • US12601518
    • 2008-03-24
    • Heeyoung LeeHyunjin YangJun Seok LeeJi Suk Choi
    • Heeyoung LeeHyunjin YangJun Seok LeeJi Suk Choi
    • C12N13/00C12N5/00
    • A61L27/3604A61L27/3683A61L2430/40
    • The present invention relates to lipid removed scaffolds(lipid-free scaffolds) for human tissue volume replacement or cell culture. More particularly, the present invention relates to a method for preparing lipid removed scaffolds(lipid-free scaffolds) for human tissue volume replacement or cell culture, the method comprising the steps of: fragmenting fat tissue to isolate lipids by ultrasonic treatment or high pressure nozzle spray; removing the isolated lipids and fat tissue from which lipids were not isolated to sterilize. According to the present invention, lipids are removed from fat tissue only by physical treatment to maintain the volume thereof and microstructures such as cellular membrane as much as possible and thus the inventive scaffolds are useful for human tissue volume replacement.
    • 本发明涉及用于人组织体积替换或细胞培养的脂质移除的支架(无脂质支架)。 更具体地说,本发明涉及一种用于制备用于人体组织体积置换或细胞培养的脂质去除的支架(无脂质支架)的方法,所述方法包括以下步骤:通过超声处理或高压喷嘴使脂肪组织分离以分离脂质 喷雾; 去除未分离脂质的分离的脂质和脂肪组织进行灭菌。 根据本发明,仅通过物理处理从脂肪组织中除去脂质以尽可能多地维持其体积和微细结构如细胞膜,因此本发明的支架可用于人体组织体积替换。
    • 7. 发明授权
    • Proliferation culture methods using micro-scaffolds for regulations of cell-to-cell signals
    • 使用微支架进行细胞 - 细胞信号调控的增殖培养方法
    • US08399253B2
    • 2013-03-19
    • US12598032
    • 2007-04-28
    • Hyunjin Yang
    • Hyunjin Yang
    • C12N5/02
    • C12N5/0075C12N2533/40
    • A three-dimensional cell culture method for increasing cell proliferation efficiency by suitably regulating the proliferation-inducing and proliferation-inhibitory signals between cells is provided. The method includes repeatedly performing any one or both of the following processes a) and b) so as to regulate proliferation-inducing and proliferation-inhibitory signals between the cells: a) a process of gradually adding the micro-scaffolds, in which a small amount of the micro-scaffolds are used in an initial stage in order to maintain a suitable distance between the cells, and the amount of the micro-scaffolds is then increased slowly according to cell proliferation rate; and b) a periodic shaking process, in which shaking is performed in order to separate connected cells from each other by a physical force, after the cells are incubated for more than a given period of time.
    • 提供了通过适当调节细胞之间的增殖诱导和增殖抑制信号来提高细胞增殖效率的三维细胞培养方法。 所述方法包括重复进行以下过程a)和b)中的任何一个或两个,以便调节细胞之间的增殖诱导和增殖抑制信号:a)逐渐加入微支架的过程,其中小支架 在初始阶段使用微支架的量以保持细胞之间的适当距离,然后根据细胞增殖速率缓慢增加微支架的量; 和b)周期性振荡过程,其中在细胞孵育超过给定时间段之后,进行摇动以便通过物理力将相互连接的细胞彼此分离。