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1. 发明授权

US5403706A Carrier matrix with dissolvably impregnated reagent 失效
标题翻译：载体基质与溶解性浸渍试剂
公开(公告)号：US5403706A
公开(公告)日：1995-04-04
申请号：US383759
申请日：1989-07-20
申请人： Hans-Erich Wilk , Dieter Mangold , Rolf Lerch , Joachim Steinbiss
发明人： Hans-Erich Wilk , Dieter Mangold , Rolf Lerch , Joachim Steinbiss
IPC分类号： C12N11/08 , C12Q1/34 , G01N21/78 , G01N33/52 , G01N33/543 , G01N33/552 , C12Q1/00 , C12N11/06 , G01N33/53
CPC分类号： G01N33/525 , C12N11/08 , G01N33/54393 , G01N33/552 , Y10S530/815
摘要： A carrier matrix of polyvinyl alcohol-coated glass is dissolvably impregnated with reagent. The matrix is manufactured by slurrying glass fibers in an excess of water and polyvinyl alcohol and forming a layer, which is then dried and impregnated with reagent.
摘要翻译：聚乙烯醇涂覆的玻璃的载体基质可溶剂地浸渍试剂。该基体通过将玻璃纤维在过量的水和聚乙烯醇中制浆并形成一层来制造，然后将其干燥并用试剂浸渍。

2. 发明授权

US5262067A Device and its use for the separation of plasma from whole blood 失效
标题翻译：装置及其用于从全血分离血浆的用途
公开(公告)号：US5262067A
公开(公告)日：1993-11-16
申请号：US698461
申请日：1991-05-10
申请人： Hans-Erich Wilk , Peter Vogel , Rolf Lerch , Erich Schneider , Andreas Marschall
发明人： Hans-Erich Wilk , Peter Vogel , Rolf Lerch , Erich Schneider , Andreas Marschall
IPC分类号： A61K35/16 , B01D39/00 , B01D39/20 , G01N1/28 , G01N33/48 , G01N33/49 , G01N33/52 , G01N33/543 , B06D15/00
CPC分类号： G01N33/491 , B01D39/00 , B01D39/2017 , G01N33/525 , G01N33/54386 , B01D2239/0478 , B01D2239/0492 , B01D2239/1233 , Y10T436/25375
摘要： The invention concerns a device and its use for separating plasma from whole blood by means of a fibre-containing layer which contains an erythrocyte-aggregating substance and is characterized in that the fibre-containing layer contains glass fibres which are coated with polyvinyl alcohol or with polyvinyl alcohol/polyvinyl acetate. The invention also concerns a test carrier for the determination of a blood component as well as a process for separation from whole blood in which the device according to the present invention is used.
摘要翻译：本发明涉及一种装置及其用于通过含有红细胞聚集物质的含纤维层从全血中分离血浆的用途，其特征在于，所述含纤维层含有玻璃纤维，所述玻璃纤维用聚乙烯醇或与聚乙烯醇/聚乙酸乙烯酯。本发明还涉及用于测定血液成分的测试载体以及使用根据本发明的装置的全血分离方法。

3. 发明授权

US5071746A Test carrier for analysis of a sample liquid, method of using a test carrier and process for making a test carrier layer 失效
标题翻译：用于分析样品液体的试验载体，使用试验载体的方法和制备试验载体层的方法
公开(公告)号：US5071746A
公开(公告)日：1991-12-10
申请号：US337351
申请日：1989-04-13
申请人： Hans-Erich Wilk , Karin Munter , Rolf Lerch
发明人： Hans-Erich Wilk , Karin Munter , Rolf Lerch
IPC分类号： G01N31/22 , G01N33/543
CPC分类号： G01N33/54366 , G01N33/54386
摘要： The present invention provides a test carrier for the analytical determination of a component of a sample liquid with the help of immunological reagents having a conjugate layer (2, 12), which contains a soluble conjugate of a first immunological binding partner with a labelling, a porous fixing layer (3, 13), which contains a second binding partner in carrier-fixed form binding specifically with the first binding partner, and an optical detection layer (8, 18), wherein the fixing layer (3, 13) is constructed in such a manner that the suction speed with which the liquid is transported in it is so high that the time needed for the spreading out of the liquid in the fixing layer is considerably shorter than the incubation time which is needed for the completion of the specific binding reaction between the first binding partner and the second binding partner and the optical detection layer (8, 18) is so fixed on the test carrier (1, 11) that, in a first position, it is not in contact with the fixing layer (3, 13) making possible a liquid exchange but can be brought into a second position in which a liquid exchange between the fixing layer (3, 13) and the optical detection layer (8, 18) can take place.The present invention also provides a process for the immunological analytical determination of a component of a sample liquid, as well as a process for the production of a fixing layer for a test carrier.
摘要翻译：本发明提供了一种用于借助具有共轭层（2,12）的免疫试剂来分析测定样品液体组分的测试载体，其包含具有标记的第一免疫结合配偶体的可溶性缀合物，多孔固定层（3,13），其包含与第一结合对象特异性结合的载体固定形式的第二结合对象，以及光学检测层（8,18），其中构成固定层（3,13）以这样一种方式使得液体在其中被输送的吸入速度非常高，使得定影层中的液体展开所需的时间比完成特定的时间所需的孵育时间短得多第一结合对象和第二结合对象之间的结合反应和光学检测层（8,18）固定在测试载体（1,11）上，在第一位置，它不与所述固定层（3,13）能够进行液体交换，但可以进入其中可以发生固定层（3,13）和光学检测层（8,18）之间的液体交换的第二位置。本发明还提供了用于对样品液体的组分进行免疫分析测定的方法，以及用于制备测试载体的固定层的方法。

4. 发明授权

US5424220A Analysis element and method for determination of an analyte in a liquid sample 失效
标题翻译：用于测定液体样品中分析物的分析元素和方法
公开(公告)号：US5424220A
公开(公告)日：1995-06-13
申请号：US10985
申请日：1993-01-29
申请人： Ada Goerlach-Graw , Ulrich Naegele , Hans-Erich Wilk , Reiner Graw
发明人： Ada Goerlach-Graw , Ulrich Naegele , Hans-Erich Wilk , Reiner Graw
IPC分类号： G01N33/543 , G01N33/558
CPC分类号： G01N33/558 , Y10S435/805 , Y10S435/81 , Y10S435/967 , Y10S435/97 , Y10S435/973 , Y10S436/805 , Y10S436/807 , Y10S436/81
摘要： The invention addresses an analysis element for the determination of an analyte according to the principle of a heterogeneous immunoassay. Said analysis element is made of a chromatographic porous carrier material and has a reaction zone, at least two detection zones and absorptive zones following said detection zones. The reaction zone contains analyte-specific and labelled binding partners which are present in a number of soluble compartments which are close together, but nevertheless spatially separated. The detection zones are adjacent to the reaction zones and contain an immobilized binding reagent for one of the binding partners present in the reaction zone. At least one detection zone contains a binding reagent for the analyte-specific unlabelled binding partner.
摘要翻译：本发明解决了根据异源免疫测定原理确定分析物的分析元件。所述分析元件由色谱多孔载体材料制成，并且具有反应区域，在所述检测区域之后具有至少两个检测区域和吸收区域。反应区含有分析物特异性和标记的结合配偶体，它们存在于多个可靠的隔室中，它们靠近在一起，但仍然在空间上分离。检测区域与反应区域相邻并且含有用于存在于反应区域中的一个结合配偶体的固定化结合试剂。至少一个检测区域包含用于分析物特异性未标记结合配偶体的结合试剂。

5. 发明授权

US5037736A Process and test carrier for the determination of an analyte 失效
标题翻译：用于测定分析物的工艺和测试载体
公开(公告)号：US5037736A
公开(公告)日：1991-08-06
申请号：US122185
申请日：1987-11-18
申请人： Helmut Freitag , Hans-Erich Wilk , Anselm Rothe
发明人： Helmut Freitag , Hans-Erich Wilk , Anselm Rothe
IPC分类号： G01N33/52 , G01N33/543
CPC分类号： G01N33/525 , G01N33/54386 , Y10S436/823
摘要： The present invention provides a process for the determination of an analyte in a body fluid, in which there are used two binding components capable of specifically binding with one another, one of the binding components being enzyme-labelled and not carrier-fixed and the other binding component being carrier-fixed. The process contains a step in which the binding components are incubated with one another so that binding reaction takes place. The amount of enzyme-labelled binding component not bound to the carrier-fixed binding component is a measure of the concentration of the analyte which is determined by allowing the labelling enzyme to act upon a substrate producing a detection signal. During the specific binding reaction, incubation is carried out simultaneously with a non-fixed substrate of the labelling enzyume which does not produce a detection signal and with a carrier-fixed substrate of the labelling enzyme which produces a detection signal, the substrate not producing a detection signal being so chosen with regard to the amount used and affinity to the labelling enzyme in relation to the amount of the substrate producing a detection signal and its affinity to the labelling enzyme and in relation to the total activity of the labelling enzyme that the enzyme-catalyzed reaction of the substrate producing a detection signal is delayed until the specific binding reaction between the binding components has substantially taken place.
摘要翻译：本发明提供了一种用于测定体液中分析物的方法，其中使用能够彼此特异性结合的两种结合成分，一种结合成分是酶标记的而不是载体固定的，另一种绑定组件是载体固定的。该方法包含将结合组分彼此温育以进行结合反应的步骤。未结合载体固定结合组分的酶标记的结合成分的量是通过使标记酶作用于产生检测信号的底物而确定的分析物的浓度的量度。在特异性结合反应期间，与不产生检测信号的标记酶的非固定底物和产生检测信号的标记酶的载体固定底物同时进行孵育，所述底物不产生相对于产生检测信号的底物量及其对标记酶的亲和性以及与标记酶的总活性相关的所用量和对标记酶的亲和性，检测信号被选择为酶产生检测信号的底物的催化反应被延迟直到基本上发生结合成分之间的特异性结合反应。

6. 发明授权

US5314803A Process and test carrier for the determination of an enzyme from an isoenzyme mixture 失效
标题翻译：用于从同工酶混合物中测定酶的工艺和测试载体
公开(公告)号：US5314803A
公开(公告)日：1994-05-24
申请号：US989875
申请日：1992-12-10
申请人： Hans-Erich Wilk , Anselm Rothe , Erich Schneider
发明人： Hans-Erich Wilk , Anselm Rothe , Erich Schneider
IPC分类号： C12Q1/40 , C12Q1/00 , G01N33/573 , G21F9/28 , C12Q1/25 , G01N21/00
CPC分类号： G01N33/573 , C12Q1/007 , Y10S435/962 , Y10S435/969 , Y10S435/97
摘要： The present invention provides a process for the determination of an enzyme from an isoenzyme mixture in a liquid sample by inhibition of the disturbing isoenzymes and determination of the non-inhibited enzyme, wherein the isoenzyme mixture is contacted with one or more substances which are able to inhibit the disturbing isoenzymes, the sample containing the inhibiting substance(s) is transferred to a small-pored reaction medium and the disturbing enzyme is there inhibited and the determination of the non-inhibited enzyme is carried out in the resulting liquid.The present invention also provides a test carrier for carrying out this process.
摘要翻译：本发明提供了一种通过抑制干扰同功酶和测定非抑制酶来测定液体样品中的同工酶混合物的酶的方法，其中同位素混合物与一种或多种能够抑制干扰的同工酶，将含有抑制物质的样品转移到小孔反应介质中，并且干扰酶被抑制，并且在所得液体中进行非抑制酶的测定。本发明还提供了一种用于执行该过程的测试载体。

7. 发明授权

US5132208A Test carrier for the analysis of a sample liquid 失效
标题翻译：用于分析样品液体的试验载体
公开(公告)号：US5132208A
公开(公告)日：1992-07-21
申请号：US189782
申请日：1988-05-03
申请人： Helmut Freitag , Hans-Erich Wilk , Anselm Rothe , Heino Eikmeier
发明人： Helmut Freitag , Hans-Erich Wilk , Anselm Rothe , Heino Eikmeier
IPC分类号： G01N31/22 , C12Q1/00 , G01N33/52 , G01N33/543
CPC分类号： G01N33/525
摘要： The present invention provides a test carrier for the analysis of a sample liquid and especially of a body fluid, having a porous test layer (8, 13) which contains a solid component (9, 17), wherein the solid component (9, 17) is coated with a protein which is insoluble in the sample liquid under the test conditions. A process for the production of this test layer is also disclosed, wherein the protein is dissolved in a solvent under conditions under which the solubility of the protein is sufficiently high in order to dissolve a certain minimum amount of the protein, the solid component of the test layer is contracted with the solution and the solubility is reduced to such an extent that the component is coated by the precipitating protein.
摘要翻译：本发明提供了一种用于分析样品液体，特别是体液的测试载体，其具有含有固体组分（9,17）的多孔测试层（8,13），其中固体组分（9,17））在测试条件下涂覆有不溶于样品液体的蛋白质。还公开了用于生产该测试层的方法，其中将蛋白质溶解在溶解有蛋白质的溶解度足够高以便溶解某些最低量的蛋白质的条件下，将固体成分试验层与溶液一起收缩，溶解度降低至组分被沉淀蛋白包被的程度。

8. 发明授权

US5232663A Test carrier for analytical determination having a highly effective fixing layer for flow-through bound/free separation 失效
标题翻译：用于分析确定的测试载体，具有高效的固定层，用于流动 - 通过边界/自由分离
公开(公告)号：US5232663A
公开(公告)日：1993-08-03
申请号：US697566
申请日：1991-05-09
申请人： Hans-Erich Wilk , Erich Schneider , Andreas Marschall , Manfred Bleisteiner
发明人： Hans-Erich Wilk , Erich Schneider , Andreas Marschall , Manfred Bleisteiner
IPC分类号： G01N31/22 , G01N33/543
CPC分类号： G01N33/54386 , Y10S436/805 , Y10S436/81
摘要： Test carrier for the analytical determination of a constituent of a sample fluid by a specific binding reaction between two binding partners having biological affinity. A plurality of test layers is so disposed that they are wetted successively by the fluid and form a fluid transport path, in which one of the test layers is a fixing layer (18) which is a porous carrier matrix. The first of the two binding partners is fixed to the latter. The fluid containing the second binding partner flows through the fixing layer (18), at right angles to its surface, on the test carrier (10).A largely complete binding reaction in an extraordinarily short time is achieved during the flow through the preferably very thin fixing layer (18) by the fact that a microporous plastics layer with a pore size P of at least 0.01 pm is used as the carrier matrix, and at the same time the size D of the second binding partner and the pore size P of the plastics layer are adjusted to one another so that the pore size P is at least twice and at most ten times as great as the size D of the second binding partner.
摘要翻译：用于通过具有生物亲和力的两个结合配偶体之间的特异性结合反应来分析测定样品液体成分的测试载体。多个测试层被布置成使得它们被流体依次润湿并形成流体输送路径，其中一个测试层是作为多孔载体矩阵的固定层（18）。两个具有约束力的合作伙伴中的第一个是固定的。含有第二结合对象的流体在测试载体（10）上流过固定层（18），与其表面成直角。在通过优选非常薄的固定层（18）的流动期间，通过使用具有至少0.01μm的孔径P的微孔塑料层作为载体矩阵的事实，在非常短的时间内实现了基本上完全的结合反应，并且同时将第二结合配对物的尺寸D和塑料层的孔径P彼此调节，使得孔径P至少为D的尺寸的两倍和至多十倍第二个约束力的伙伴。

9. 发明授权

US5162238A Test carrier for analysis of a liquid sample 失效
标题翻译：用于液体样品分析的试验载体
公开(公告)号：US5162238A
公开(公告)日：1992-11-10
申请号：US275409
申请日：1988-11-23
申请人： Henio Eikmeier , Helmut Freitag , Karin Munter , Klaus Pollmann , Hans-Erich Wilk , Johannes Winkle
发明人： Henio Eikmeier , Helmut Freitag , Karin Munter , Klaus Pollmann , Hans-Erich Wilk , Johannes Winkle
IPC分类号： G01N31/22 , G01N33/543 , G01N33/551 , G01N33/552
CPC分类号： G01N33/54386 , G01N33/551 , G01N33/552
摘要： The present invention provides a test carrier for the analysis of a sample liquid with a test layer which contains a ligand fixed to to carrier particles, which ligand reacts with a component of the sample liquid, the test layer having a three-dimensional open structure which is so porous that the sample liquid with the component can penetrate into it, the carrier particles being arranged in the layer in such a manner that the ligand, upon pentration of the sample liquid, comes into contact with the component of the sample, wherein the carrier particles consist of an inorganic material, on the surface of which reactive organic groups are covalently bound as coupling groups, and the ligand is covalently bound to the coupling groups. The present invention also provides processes for the production of such a test carrier and a method for using it.
摘要翻译：本发明提供了一种用于分析具有测试层的样品液体的测试载体，该测试层含有固定在载体颗粒上的配体，该配体与样品液体的组分反应，测试层具有三维开放结构，使得具有组分的样品液体可以渗入其中，载体颗粒以这样的方式排列在层中，使得配体在样品液体的稀释时与样品的组分接触，其中，载体颗粒由无机材料组成，其表面上的反应性有机基团共价键合作为偶联基团，并且配体与偶联基团共价结合。本发明还提供了生产这种试验载体的方法及其使用方法。

10. 发明授权

US5037764A Process for determination of an analyte and reagent therefor 失效
标题翻译：用于测定分析物和试剂的方法
公开(公告)号：US5037764A
公开(公告)日：1991-08-06
申请号：US73989
申请日：1987-07-16
申请人： Hans-Erich Wilk , Helmut Freitag , Josef Burg , Johann Berger
发明人： Hans-Erich Wilk , Helmut Freitag , Josef Burg , Johann Berger
IPC分类号： G01N33/58 , C12Q1/00 , G01N33/543 , G01N33/78
CPC分类号： G01N33/78 , G01N33/54306
摘要： The present invention provides a process for the detection of an analyte, wherein a sample containing the analyte is incubated either with a labelled binding partner and the immobilized analyte or an immobilized analyte analogue or with the labelled analyte or analyte analogue and an immobilized binding partner, the binding partner displaying towards the immobilized or labelled analyte or analyte analogue a higher affinity than towards the free analyte, and the labelled binding partner or the labelled analyte or the labelled analyte analogue is present in insufficiency compared with the concentration of the analyte to be detected.The present invention also provides an agent for carrying out this process, wherein it contains either the immobilized analyte or an immobilized analyte analogue and a labelled binding partner or the labelled analyte or a labelled analyte analogue and an immobilized binding partner, as well as optionally an appropriate buffer system and further conventionally used adjuvants, the binding partner displaying towards the immobilized or labelled analyte or analyte analogue a higher affinity than towards the free analyte.
摘要翻译：本发明提供了一种用于检测分析物的方法，其中将含有分析物的样品与标记的结合配偶体和固定的分析物或固定的分析物类似物或标记的分析物或分析物类似物和固定的结合配偶体一起温育，与被检测的分析物的浓度相比，向固定的或标记的分析物或分析物类似物的结合配体显示出比对于游离的分析物更高的亲合力，并且标记的结合配偶体或标记的分析物或标记的分析物类似物的存在不足。本发明还提供了用于进行该方法的试剂，其中其包含固定的分析物或固定的分析物类似物和标记的结合配偶体或标记的分析物或标记的分析物类似物和固定的结合配偶体，以及任选的合适的缓冲系统和进一步常规使用的佐剂，向固定的或标记的分析物或分析物类似物的结合配偶体显示比对游离的分析物更高的亲和力。

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