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1. 发明授权

US4054557A Growth promoting polypeptides and preparation method 失效
标题翻译：生长促进多肽及其制备方法
公开(公告)号：US4054557A
公开(公告)日：1977-10-18
申请号：US470040
申请日：1974-05-15
申请人： Hans Uno Sievertsson , Linda Fryklund , Knut Oivind Uthne
发明人： Hans Uno Sievertsson , Linda Fryklund , Knut Oivind Uthne
IPC分类号： A61K38/00 , C07K14/475 , C08H1/00
CPC分类号： C07K14/475 , A61K38/00 , Y10S530/831
摘要： The application discloses (A) a growth-promoting polypeptide having molecular weight from about 5,000 to about 7,000 chromatographic mobility under electrophoresis from about 0.25 to about 0.37 relative to lysine at pH 5 and from about 0.17 to about 0.43 relative to aspartic acid at pH 7.5, terminal amino acid at its amino end being asparagine or aspartic acid, and respective amounts of aspartic acid, threonine, serine, glutamic acid, proline, glycine, alanine, valine, leucine, tyrosine, phenylalanine, lysine, arginine, trypophan, and cysteine or carboxymethyl cysteine, and also a couple of instances also methionine, isoleucine and histidine; and (B) the derivation of the polypeptides from blood serum or plasma or a plasma fraction by combined steps of (a) homogenizing the starting material in water, (b) admixing a solution of hydrochloric acid in ethanol thereby splitting the polypeptide from its carrier proteins and precipitating them, (c) adjusting the pH to 8.4 to provide a precipitate, (d) separating it, (e) adjusting the pH of the liquid residue to 3, (f) admixing a water-insoluble strongly acidic cation exchange matrix of sulfopropyl-oxygen-linked-substitued dextran cross-linked with epichlorhydrin at pH 3, (g) separating the gel matrix from the liquid, (h) eluting the polypeptide-containing material from the matrix with (x) ammonium acetate at pH 10 or (y) 0.75M ammonium bicarbonate; and (i) precipitating the polypeptide by admixing acetone.
摘要翻译：本申请公开了（A）相对于PH7.5的天冬氨酸，相对于pH5的赖氨酸和约0.17-约0.43的分子量约为5,000至约7000的色谱迁移率，生物促进多肽的电泳从约0.25至约0.37 氨基末端氨基酸为天冬酰胺或天冬氨酸，各自的天冬氨酸，苏氨酸，丝氨酸，谷氨酸，脯氨酸，甘氨酸，丙氨酸，缬氨酸，亮氨酸，酪氨酸，苯丙氨酸，赖氨酸，精氨酸，trypophan和半胱氨酸或羧甲基半胱氨酸，还有一些情况，例如甲硫氨酸，异亮氨酸和组氨酸; 和（B）通过以下步骤的组合步骤（a）在水中均化原料，（b）将盐酸在乙醇中的溶液混合，从而将多肽从其载体中分离出来，从血清或血浆或血浆级分衍生多肽蛋白质并沉淀，（c）将pH调节至8.4以提供沉淀物，（d）将其分离，（e）将液体残余物的pH调节至3，（f）混合不溶于水的强酸性阳离子交换基质（g）将凝胶基质与液体分离，（h）用pH为10的（x）乙酸铵从基质中洗脱含有多肽的物质，或（y）0.75M碳酸氢铵; 和（i）通过混合丙酮沉淀多肽。

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