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1. 发明授权

US07332274B2 Process of quality examining for microarray of biological material 失效
标题翻译：生物材料微阵列的质量检验过程
公开(公告)号：US07332274B2
公开(公告)日：2008-02-19
申请号：US10180961
申请日：2002-06-27
申请人： Han-Oh Park , Jin-Tae Jeon , Gui-Hwan Oh , Jae-don Lee
发明人： Han-Oh Park , Jin-Tae Jeon , Gui-Hwan Oh , Jae-don Lee
IPC分类号： C12Q1/68 , C12M1/34 , C12M3/00 , G01N33/543 , G01N33/551 , G01N21/00 , G01N21/75 , G01N33/53
CPC分类号： G01N21/6452 , G01N21/253 , G01N21/274 , G01N21/6428 , G01N21/76 , G01N33/551 , G01N2021/6417 , G01N2021/6441 , G01N2021/646 , G01N2035/00158 , Y10S436/80 , Y10S436/809
摘要： The present invention relates a process of quality examining for microarray of biological material. More particularly, the present invention is directed to a process of quality examining for microarray of biological material, which comprises 1) a step for mixing probe and a compound which emits light or heat and does not react with said probe, 2) a step for microarraying the mixture obtained in step 1) on a substrate, and 3) a step for measuring light or heat emitted by the scanning of each spots of the microarray mixture.
摘要翻译：本发明涉及生物材料微阵列的质量检验方法。更具体地说，本发明涉及生物材料微阵列的质量检验方法，该方法包括：1）将探针和发出光或热而不与所述探针反应的化合物混合的步骤，2）步骤将步骤1）中获得的混合物微阵列在基底上，和3）用于测量通过扫描微阵列混合物的每个斑点而发射的光或热的步骤。

2. 发明授权

US06849404B2 Polymerase chain reaction of DNA of which base sequence is completely unidentified 失效
标题翻译： DNA序列的聚合酶链反应完全不明确
公开(公告)号：US06849404B2
公开(公告)日：2005-02-01
申请号：US09849597
申请日：2001-05-07
申请人： Han Oh Park , Se-Yeon Weon , Joo-Won Rhee , In-Suk Joung , Su-Nam Song , Jin-Tae Jeon
发明人： Han Oh Park , Se-Yeon Weon , Joo-Won Rhee , In-Suk Joung , Su-Nam Song , Jin-Tae Jeon
IPC分类号： C12N15/09 , C12Q1/68 , C07H21/04 , C12P19/34
CPC分类号： C12Q1/686 , C12Q2525/301 , C12Q2525/191 , C12Q2565/30
摘要： The present invention relates to a process for amplifying DNA of an organism. More particularly, the present invention is directed to a process for amplifying DNA of an organism through Polymerase Chain Reaction(PCR) without any information regarding a primer needed for amplifying DNA of an organism.
摘要翻译：本发明涉及一种用于扩增生物体的DNA的方法。更具体地，本发明涉及通过聚合酶链式反应（PCR）扩增生物体的DNA的方法，而没有关于扩增生物体的DNA所需的引物的任何信息。

3. 发明授权

US06653108B2 Process for selective amplification of a full-length cDNA involving an anchor nucleic acid 有权
标题翻译：选择性扩增涉及锚定核酸的全长cDNA的方法
公开(公告)号：US06653108B2
公开(公告)日：2003-11-25
申请号：US09989706
申请日：2001-11-21
申请人： Han-Oh Park , Jin-Tae Jeon , Mi-Sook Jang
发明人： Han-Oh Park , Jin-Tae Jeon , Mi-Sook Jang
IPC分类号： C12Q168
CPC分类号： C12Q1/6806 , C12N15/1096 , C12Q1/686 , C12Q2531/113 , C12Q2525/191 , C12Q2521/107
摘要： The present invention relates to a process for the preparation of full-length complementary DNA (cDNA). More particularly, the present invention is directed to a process for selective amplification of full-length cDNA, which comprises: i) a step for preparing a hybrid composed of a messenger RNA (mRNA) strand and a cDNA strand of which three (3) or four (4) deoxycitidinemono phosphate (dCMP) are combined at 3′ end, by treating mRNA with reverse transcriptase; separately from the above step, ii) a step for adenylating single strand anchor of which biotin or phosphate group is combined at 3′ end, and phosphate group is combined at 5′ end; iii) a step for ligating said adenylated single strand anchor to 3′ end of full-length cDNA strand of said cDNA/mRNA hybrid to select full-length cDNA/mRNA hybrid; and iv) a step for amplifying only the full-length cDNA/mRNA hybrid through polymerase chain reaction (PCR) which employs a primer of which base sequence is complementary to that of said anchor.
摘要翻译：本发明涉及一种制备全长互补DNA（cDNA）的方法。更具体地，本发明涉及全长cDNA的选择性扩增方法，其包括：i）制备由信使RNA（mRNA）链和cDNA链组成的杂交体的步骤，其中三（3）或4（4）脱氧皮质素磷酸（dCMP）通过用逆转录酶处理mRNA在3'末端组合; ii）在3'端结合生物素或磷酸基团的单链锚定的腺苷酸化步骤，在5'末端结合磷酸基; iii）将所述腺苷酸化的单链锚定于所述cDNA / mRNA杂交体的全长cDNA链的3'末端以选择全长cDNA / mRNA杂交体的步骤; 和iv）通过聚合酶链式反应（PCR）仅扩增全长cDNA / mRNA杂交体的步骤，其使用与所述锚的碱基序列互补的引物。

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