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1. 发明专利

JP2007222052A Method for inducing shoot 有权
标题翻译：诱导鞋的方法
公开(公告)号：JP2007222052A
公开(公告)日：2007-09-06
申请号：JP2006045690
申请日：2006-02-22
申请人： Hamamatsu Photonics Kk , Toyota Motor Corp , トヨタ自動車株式会社 , 浜松ホトニクス株式会社
发明人： KIMURA TATSURO , TSUCHIYA KOJI , TACHIIRI YOSHIAKI
IPC分类号： A01H4/00
摘要： PROBLEM TO BE SOLVED: To provide a method for inducing the shoots of a plant.
SOLUTION: The method for inducing the shoots of the plant is characterized by culturing the cells, organs or tissues of a plant under light quality conditions comprising the light wavelengths of blue light of 420 to 500 nm, green light of 500 to 570 nm, or red light of 590 to 700 nm and a light intensity of 2 to 50 μmol/m
2 /s.
COPYRIGHT: (C)2007,JPO&INPIT
摘要翻译：要解决的问题：提供诱导植物芽的方法。解决方案：诱导植物芽的方法的特征在于在包括420-500nm的蓝光的光波长，500-570的绿光的光质量条件下培养植物的细胞，器官或组织或红色光为590〜700nm，光强度为2〜50μmol/ m 2 SP / s。版权所有（C）2007，JPO＆INPIT

2. 发明专利

JP2007043924A Method for judging methylated state in dna 有权
标题翻译：在DNA中判断甲基化状态的方法
公开(公告)号：JP2007043924A
公开(公告)日：2007-02-22
申请号：JP2005229907
申请日：2005-08-08
申请人： Hamamatsu Photonics Kk , 浜松ホトニクス株式会社
发明人： TACHIIRI YOSHIAKI , UCHIDA TETSUYA
IPC分类号： C12Q1/68 , C12N15/09
摘要： PROBLEM TO BE SOLVED: To provide a method for easily judging the methylated state in a specific domain in a DNA with different methylated states obtained from a cell cluster or the like in a single operation without making any cloning.
SOLUTION: The method comprises the first step of making a chemical treatment of a DNA to convert nonmethylated cytosine to urasil, the second step of amplifying a double-stranded DNA fragment corresponding to a specific domain by PCR, the third step of synthesizing a single-stranded DNA fragment by PCR using a reaction liquid containing one primer, dNTP (deoxynucleoside-5'-triphosphate) and ddCTP (dideoxycytidine-5'-triphosphate) with the amplified double-stranded DNA fragment as template, the fourth step of isolating the synthesized single-stranded DNA fragment and detecting it by capillary electrophoresis, and the fifth step of judging that the cytosine corresponding to a peak position detected is methylated.
COPYRIGHT: (C)2007,JPO&INPIT
摘要翻译：待解决的问题：提供一种在不进行任何克隆的情况下，在单次操作中容易地判断从细胞簇等获得的具有不同甲基化状态的DNA的特定区域中的甲基化状态的方法。解决方案：该方法包括进行DNA的化学处理以将非甲基化胞嘧啶转化成尿嘧啶的第一步骤，通过PCR扩增对应于特定结构域的双链DNA片段的第二步，第三步合成通过PCR使用含有一个引物，dNTP（脱氧核苷-5'-三磷酸）和ddCTP（双脱氧胞苷-5'-三磷酸））的反应液与扩增的双链DNA片段作为模板的单链DNA片段，第四步分离合成的单链DNA片段并通过毛细管电泳检测，第五步判断与检测到的峰位置相对应的胞嘧啶是甲基化的。版权所有（C）2007，JPO＆INPIT

3. 发明专利

JP2012070727A Method for increasing nutrient component content per unit dry weight of tomato fruit 审中-公开
标题翻译：增加TOMATO水果单位干重的营养成分含量的方法
公开(公告)号：JP2012070727A
公开(公告)日：2012-04-12
申请号：JP2011097344
申请日：2011-04-25
申请人： Hamamatsu Photonics Kk , 浜松ホトニクス株式会社
发明人： UCHIDA TETSUYA , SUZUKI HITOSHI , TACHIIRI YOSHIAKI
IPC分类号： A01G7/00
摘要： PROBLEM TO BE SOLVED: To provide a method for increasing the content per unit dry weight of at least one or more kinds of components selected from the group consisting of amino acids, potassium, carotenoids, ascorbic acid and vitamin B6 in tomato fruits.SOLUTION: Far infrared light in the range of 700-750 nm wave length having the strongest light intensity is irradiated to tomatoes after the end of a light period to increase the amino acid content and potassium content per unit dry weight of the tomato fruits. To increase the carotenoid content, ascorbic acid content and vitamin B6 content per unit dry weight of the tomato fruits, far infrared light in the range of 600-700 nm wave length having the strongest light intensity is irradiated to tomatoes.
摘要翻译：待解决的问题：提供一种增加番茄果实中选自氨基酸，钾，类胡萝卜素，抗坏血酸和维生素B6中的至少一种或多种成分的单位干重量的方法。
解决方案：在光周期结束后，将具有最强光强度的700-750nm波长范围内的远红外光照射到番茄，以增加番茄的每单位干重的氨基酸含量和钾含量水果。为了增加番茄果实的每单位干重中的类胡萝卜素含量，抗坏血酸含量和维生素B6含量，将具有最强光强度的600-700nm波长范围内的远红外光照射到番茄上。版权所有（C）2012，JPO＆INPIT

4. 发明专利

JP2015000021A 細胞内ｃＡＭＰ濃度を制御する方法审中-公开
标题翻译：控制细胞内cAMP浓度的方法
公开(公告)号：JP2015000021A
公开(公告)日：2015-01-05
申请号：JP2013125373
申请日：2013-06-14
申请人：浜松ホトニクス株式会社 , Hamamatsu Photonics Kk
发明人： TAKEBE MASUMI , MATSUNAGA SHIGERU , TAKAMOTO HISANOBU , TACHIIRI YOSHIAKI
IPC分类号： C12N5/10 , C12N1/15 , C12N1/19 , C12N1/21 , C12N15/09 , C12Q1/02 , C12Q1/66
摘要：【課題】細胞内cAMP濃度を時間的に自由自在に制御する方法を提供すること。【解決手段】光活性化アデニル酸シクラーゼ遺伝子及び改変ルシフェラーゼ遺伝子を導入した細胞であって、改変ルシフェラーゼ遺伝子によってコードされる改変ルシフェラーゼは、cAMP結合ドメインが挿入されており、cAMPのcAMP結合ドメインへの結合により、改変ルシフェラーゼがルシフェリンと反応可能となるようタンパク質構造が変化し、改変ルシフェラーゼの発光量が増大する、細胞を利用する。【選択図】なし
摘要翻译：要解决的问题：提供一种随意控制细胞内cAMP浓度的方法。解决方案：本发明的方法利用已经引入可光活化的腺苷酸环化酶基因和修饰的萤光素酶基因的细胞，其中编码的修饰的萤光素酶通过修饰的荧光素酶基因含有插入的cAMP结合结构域，并且只有当cAMP与cAMP结合结构域结合时，修饰的荧光素酶的蛋白质结构发生变化并变成与荧光素反应，以促进经修饰的荧光素酶的发光。

5. 发明专利

JP2011109925A Method for controlling growth of grass plant 有权
标题翻译：控制草坪生长的方法
公开(公告)号：JP2011109925A
公开(公告)日：2011-06-09
申请号：JP2009266318
申请日：2009-11-24
申请人： Hamamatsu Photonics Kk , 浜松ホトニクス株式会社
发明人： UCHIDA TETSUYA , TACHIIRI YOSHIAKI , SUZUKI HITOSHI
IPC分类号： A01G7/00 , A01G16/00 , C12Q1/68
摘要： PROBLEM TO BE SOLVED: To provide a method for controlling growth of grass plant by which annual harvest of the grass plant per unit area is increased, a cultivation period per single cropping is shortened and also the harvesting amount in an ordinary cultivation period can be maintained.
SOLUTION: The method for controlling growth of grass plant includes irradiating the plant with only artificial light while shielding outside light to grow the plant in a photoperiod including a light period and a dark period. In the method, end-of-day treatment for 10-15 days is subjected to the plant from one day in a period of 13-3 days before a day when the transcription amount of Hd3a in the plant or mRNA of its homologous gene reaches its peak, to one day in a period from the day when the transcription amount reaches its peak to a day 7 days after the day when the transcription amount reaches its peak, and the end-of-day treatment includes irradiating the plant with far red light in an irradiation time within 60 min just after finishing the light period.
COPYRIGHT: (C)2011,JPO&INPIT
摘要翻译：要解决的问题：为了提供一种控制草坪生长的方法，每单位面积的年收获量增加，每个单作物的种植期缩短，普通栽培期的收获量可以维护。解决方案：用于控制草植物生长的方法包括仅在人造光下照射植物，同时屏蔽外部光线以在包括光周期和暗期的光周期中使植物生长。在该方法中，在植物中的Hd3a的转录量或其同源基因的mRNA的转录量的一天之前的13-3天的一天内，从10天到10天的日处理结束其高峰期，从转录量达到峰值的当天到转录量达到峰值的第7天为止的一天，并且日终处理包括以远红色照射植物在完成光照期后的60分钟内照射时间。版权所有（C）2011，JPO＆INPIT

6. 发明专利

AU5680398A Process for separating biological substances by using photoresist 未知
公开(公告)号：AU5680398A
公开(公告)日：1998-08-25
申请号：AU5680398
申请日：1998-02-03
申请人： HAMAMATSU PHOTONICS KK
发明人： TSUCHIYA HIROSHI , TACHIIRI YOSHIAKI , ITO TOSHIAKI
IPC分类号： C12N5/06 , C07K1/24 , C12N15/10 , C07K1/14 , C07H1/08
摘要： After a photoresist (2) is used to fix a biological sample, in order to obtain a particular biological substance, for example, a certain cell or biopolymer, from the biological sample embedded in the photoresist (2), properties of the photoresist are changed by exposing the portion of the photoresist (2) that covers the biological substance to light L which has an appropriate wavelength. The biological substance embedded in the changed photoresist is collected.

7. 发明专利

JPH10215862A SEPARATION OF SUBSTANCE ORIGINATING FROM LIVING BODY BY USING PHOTORESIST 失效
公开(公告)号：JPH10215862A
公开(公告)日：1998-08-18
申请号：JP3432197
申请日：1997-02-03
申请人： HAMAMATSU PHOTONICS KK
发明人： TSUCHIYA KOJI , TACHIIRI YOSHIAKI , ITO TOSHIAKI
IPC分类号： C12N5/06 , C07K1/24
摘要： PROBLEM TO BE SOLVED: To enable the simple separation of a substance originating from living body in high purity and utilizing it for gene cloning and gene diagnosis by covering the sample containing substances originating from living body with a positive type photo resist, treating them under specific conditions and covering the living body-originating substances therefrom. SOLUTION: A sample from living body containing substances originating from loving body as DNA, protein and the like is coated with a positive type photoresist as photomodification type and the photo resist is solidified to fix the sample from living body, the part of covering the living body substance is exposed to light, the photoresist exposed to light is dissolved in the dissolving liquid to recover the living body substance buried in the photoresist thereby separating the substance. The exposure to the light is preferably carried out by using visible, ultraviolet, X-ray or electron beam.

8. 发明专利

JP2000083667A CONTROL OF GENE EXPRESSION AND GENE DUPLICATION 失效
公开(公告)号：JP2000083667A
公开(公告)日：2000-03-28
申请号：JP28201198
申请日：1998-09-17
申请人： HAMAMATSU PHOTONICS KK
发明人： TACHIIRI YOSHIAKI
IPC分类号： C12N15/09
摘要： PROBLEM TO BE SOLVED: To provide a method for control of gene expression and gene duplication through the intermediary of light by binding a photodissociative compound to part of any substance involved in a control system of gene expression to thereby control the gene expression so that the gene expression is permitted at any time in any sites. SOLUTION: This method comprises: preparing a DNA sequence of the control range of a RNA polymerase, a primer corresponding to a DNA polymerase or the like each forming part of substances A, B and C involved in a control system of gene expression; binding an avidin beads, caused by a biotin-avidin bond via an orthonitrothiobenzyl ether bond, to the DNA sequence of the control range or the primer A; binding a photodissociative compound D thereto for controlling gene expression; and conducting a light radiation on the control system where the gene expression is controlled so as to remove the photodissociative compound D and activating it for gene expression, thus permitting the gene expression at any time in any cells or tissue sites and providing a control of gene expression and gene duplication by use of light.

9. 发明专利

JPH1128086A DEVICE FOR INTRODUCING MOLECULE INTO CELL 失效
公开(公告)号：JPH1128086A
公开(公告)日：1999-02-02
申请号：JP18660197
申请日：1997-07-11
申请人： HAMAMATSU PHOTONICS KK
发明人： FUJISAKA SHINICHI , TACHIIRI YOSHIAKI , SATO KATSUHIKO
IPC分类号： C12N13/00 , A61B18/00 , A61B17/36
摘要： PROBLEM TO BE SOLVED: To provide a molecule-introducing device having an excellent operation performance and not giving a damage, etc., to cells. SOLUTION: A liquid supplied from a liquid-injecting device 4 is charged in a chamber 18. Pulse laser light hν introduced from a laser device 2 is guided with an optical fiber cable 12, gathered with a light-gathering lens 30, and focused at a breakdown position in the liquid. The energy of the laser hν induces impact waves due to a breakdown phenomenon in the liquid, and is propagated to outer cells and a molecule to be introduced through the liquid and the sound-propagating member 56. The molecule is introduced into the cell with the impact waves.

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