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1. 发明授权

US08431132B2 Recombinant human EPO-FC fusion proteins with prolonged half-life and enhanced erythropoietic activity in vivo 有权
标题翻译：重组人EPO-FC融合蛋白具有延长的半衰期和体内增强的红细胞生成活性
公开(公告)号：US08431132B2
公开(公告)日：2013-04-30
申请号：US12555743
申请日：2009-09-08
申请人： Haitao Wang , Du Yong , Rui Zhang , Jing Xu , Longbin Liu
发明人： Haitao Wang , Du Yong , Rui Zhang , Jing Xu , Longbin Liu
IPC分类号： A61K39/00 , C07K1/00 , C07K16/00
CPC分类号： C07K14/505 , A61K9/0019 , A61K38/00 , A61K47/6811 , C07K2317/53 , C07K2319/30
摘要： A recombinant fusion protein comprising a human erythropoietin peptide portion linked to an immunoglobulin peptide portion is described. The fusion protein has a prolonged half-life in vivo in comparison to naturally occurring or recombinant native human erythropoietin. In one embodiment of the invention, the protein has a half-life in vivo at least three fold higher than native human erythropoietin. The fusion protein also exhibits enhanced erythropoietic bioactivity in comparison to native human erythropoietin. In one embodiment, the fusion protein comprises the complete peptide sequence of a human erythropoietin (EPO) molecule and the peptide sequence of an Fc fragment of human immunoglobulin IgG1. The Fc fragment in the fusion protein includes the hinge region, CH2 and CH3 domains of human immunoglobulin IgG1. The EPO molecule may be linked directly to the Fc fragment to avoid extraneous peptide linkers and lessen the risk of an immunogenic response when administered in vivo. In one embodiment the hinge region is a human Fc fragment variant having a non-cysteine residue at amino acid 6. The invention also relates to nucleic acid and amino acid sequences encoding the fusion protein and transfected cell lines and methods for producing the fusion protein. The invention further includes pharmaceutical compositions comprising the fusion protein and methods of using the fusion protein and/or the pharmaceutical compositions, for example to stimulate erythropoiesis in subjects in need of therapy.
摘要翻译：描述了包含与免疫球蛋白肽部分连接的人促红细胞生成素肽部分的重组融合蛋白。与天然存在的或重组的天然人促红细胞生成素相比，融合蛋白在体内具有延长的半衰期。在本发明的一个实施方案中，蛋白质的体内半衰期比天然人促红细胞生成素高至少三倍。与天然人促红细胞生成素相比，融合蛋白也表现出增强的红细胞生物活性。在一个实施方案中，融合蛋白包含人促红细胞生成素（EPO）分子的完整肽序列和人免疫球蛋白IgG1的Fc片段的肽序列。融合蛋白中的Fc片段包括人免疫球蛋白IgG1的铰链区，CH2和CH3结构域。 EPO分子可以直接连接到Fc片段，以避免外来的肽接头并且当在体内施用时减轻免疫原性应答的风险。在一个实施方案中，铰链区是在氨基酸6具有非半胱氨酸残基的人Fc片段变体。本发明还涉及编码融合蛋白和转染细胞系的核酸和氨基酸序列以及用于产生融合蛋白的方法。本发明还包括包含融合蛋白的药物组合物和使用融合蛋白和/或药物组合物的方法，例如刺激需要治疗的受试者的红细胞生成。

2. 发明授权

US07964375B2 Polynucleotides encoding human EPO-FC fusion proteins with prolonged half-life and enhanced erythropoietic activity in vivo 有权
标题翻译：编码具有延长的半衰期和增强体内红细胞生成活性的人EPO-FC融合蛋白的多核苷酸
公开(公告)号：US07964375B2
公开(公告)日：2011-06-21
申请号：US12555742
申请日：2009-09-08
申请人： Haitao Wang , Du Yong , Rui Zhang , Jing Xu , Longbin Liu
发明人： Haitao Wang , Du Yong , Rui Zhang , Jing Xu , Longbin Liu
IPC分类号： A61K39/00 , C12P21/06 , C12N1/20 , C12N15/00 , C07K14/00 , C07H21/02
CPC分类号： C07K14/505 , A61K9/0019 , A61K38/00 , A61K47/6811 , C07K2317/53 , C07K2319/30
摘要： A recombinant fusion protein comprising a human erythropoietin peptide portion linked to an immunoglobulin peptide portion is described. The fusion protein has a prolonged half-life in vivo in comparison to naturally occurring or recombinant native human erythropoietin. In one embodiment of the invention, the protein has a half-life in vivo at least three fold higher than native human erythropoietin. The fusion protein also exhibits enhanced erythropoietic bioactivity in comparison to native human erythropoietin. In one embodiment, the fusion protein comprises the complete peptide sequence of a human erythropoietin (EPO) molecule and the peptide sequence of an Fc fragment of human immunoglobulin IgG1. The Fc fragment in the fusion protein includes the hinge region, CH2 and CH3 domains of human immunoglobulin IgG1. The EPO molecule may be linked directly to the Fc fragment to avoid extraneous peptide linkers and lessen the risk of an immunogenic response when administered in vivo. In one embodiment the hinge region is a human Fc fragment variant having a non-cysteine residue at amino acid 6. The invention also relates to nucleic acid and amino acid sequences encoding the fusion protein and transfected cell lines and methods for producing the fusion protein. The invention further includes pharmaceutical compositions comprising the fusion protein and methods of using the fusion protein and/or the pharmaceutical compositions, for example to stimulate erythropoiesis in subjects in need of therapy.
摘要翻译：描述了包含与免疫球蛋白肽部分连接的人促红细胞生成素肽部分的重组融合蛋白。与天然存在的或重组的天然人促红细胞生成素相比，融合蛋白在体内具有延长的半衰期。在本发明的一个实施方案中，蛋白质的体内半衰期比天然人促红细胞生成素高至少三倍。与天然人促红细胞生成素相比，融合蛋白也表现出增强的红细胞生物活性。在一个实施方案中，融合蛋白包含人促红细胞生成素（EPO）分子的完整肽序列和人免疫球蛋白IgG1的Fc片段的肽序列。融合蛋白中的Fc片段包括人免疫球蛋白IgG1的铰链区，CH2和CH3结构域。 EPO分子可以直接连接到Fc片段，以避免外来的肽接头并且当在体内施用时减轻免疫原性应答的风险。在一个实施方案中，铰链区是在氨基酸6具有非半胱氨酸残基的人Fc片段变体。本发明还涉及编码融合蛋白和转染细胞系的核酸和氨基酸序列以及用于产生融合蛋白的方法。本发明还包括包含融合蛋白的药物组合物和使用融合蛋白和/或药物组合物的方法，例如刺激需要治疗的受试者的红细胞生成。

3. 发明授权

US07868151B2 Recombinant human interferon-like proteins 有权
标题翻译：重组人干扰素样蛋白
公开(公告)号：US07868151B2
公开(公告)日：2011-01-11
申请号：US12555762
申请日：2009-09-08
申请人： Haitao Wang , Chunsheng Mao , Jizhi Li , Jing Xu , Rui Zhang , Ling Wang , Yong Du , Longbin Liu
发明人： Haitao Wang , Chunsheng Mao , Jizhi Li , Jing Xu , Rui Zhang , Ling Wang , Yong Du , Longbin Liu
IPC分类号： C07H21/04 , A61K38/15 , C07K14/00 , C12P21/02
CPC分类号： C07K14/56 , A61K38/00 , C07K14/555
摘要： This application relates to recombinant human interferon-like proteins. In one embodiment a recombinant protein created by gene shuffling technology is described having enhanced anti-viral and anti-proliferative activities in comparison to naturally occurring human interferon alpha 2b (HuIFN-α2b). The invention encompasses a polynucleotide encoding the protein and recombinant vectors and host cells comprising the polynucleotide. Preferably the polynucleotide is selected from the group of polynucleotides each having a sequence at least 93% identical to SEQ ID: No. 1 and the protein is selected from the group of proteins each having an amino acid sequence at least 85% identical to SEQ ID No: 2. The proteins and compositions comprising the proteins can be used for treatment of conditions responsive to interferon therapy, such as viral diseases and cancer.
摘要翻译：本申请涉及重组人类干扰素样蛋白。在一个实施方案中，与天然存在的人类干扰素α2b（HuIFN-α2b）相比，描述了通过基因改组技术产生的重组蛋白质具有增强的抗病毒和抗增殖活性。本发明包括编码蛋白质的多核苷酸和包含多核苷酸的重组载体和宿主细胞。优选地，多核苷酸选自各自具有与SEQ ID：No.1至少93％相同的序列的多核苷酸，并且所述蛋白质选自蛋白质组，其各自具有与SEQ ID NO：至少85％相同的氨基酸序列否：2.包含蛋白质的蛋白质和组合物可用于治疗对干扰素治疗（例如病毒性疾病和癌症）有反应的病症。

4. 发明申请

US20080312148A1 RECOMBINANT HUMAN INTERFERON-LIKE PROTEINS 有权
标题翻译：重组人类干扰素蛋白
公开(公告)号：US20080312148A1
公开(公告)日：2008-12-18
申请号：US11764786
申请日：2007-06-18
申请人： Haitao Wang , Chunsheng Mao , Jizhi Li , Jing Xu , Rui Zhang , Ling Wang , Yong Du , Longbin Liu
发明人： Haitao Wang , Chunsheng Mao , Jizhi Li , Jing Xu , Rui Zhang , Ling Wang , Yong Du , Longbin Liu
IPC分类号： A61K38/21 , A61P35/00 , C07K14/00 , C12N1/19 , C12N1/21 , C12N15/11 , C12N15/63 , C12N5/10
CPC分类号： C07K14/56 , A61K38/00 , C07K14/555
摘要： This application relates to recombinant human interferon-like proteins. In one embodiment a recombinant protein created by gene shuffling technology is described having enhanced anti-viral and anti-proliferative activities in comparison to naturally occurring human interferon alpha 2b (HuIFN-α2b). The invention encompasses a polynucleotide encoding the protein and recombinant vectors and host cells comprising the polynucleotide. Preferably the polynucleotide is selected from the group of polynucleotides each having a sequence at least 93% identical to SEQ ID: No. 1 and the protein is selected from the group of proteins each having an amino acid sequence at least 85% identical to SEQ ID No: 2. The proteins and compositions comprising the proteins can be used for treatment of conditions responsive to interferon therapy, such as viral diseases and cancer.
摘要翻译：本申请涉及重组人类干扰素样蛋白。在一个实施方案中，与天然存在的人干扰素α2b（HuIFN-α2b）相比，描述了通过基因改组技术产生的重组蛋白质具有增强的抗病毒和抗增殖活性。本发明包括编码蛋白质的多核苷酸和包含多核苷酸的重组载体和宿主细胞。优选地，多核苷酸选自各自具有与SEQ ID：No.1至少93％相同的序列的多核苷酸，并且所述蛋白质选自蛋白质组，其各自具有与SEQ ID NO：至少85％相同的氨基酸序列否：2.包含蛋白质的蛋白质和组合物可用于治疗对干扰素治疗（例如病毒性疾病和癌症）有反应的病症。

5. 发明授权

US08425895B2 Recombinant human interferon-like proteins 有权
标题翻译：重组人干扰素样蛋白
公开(公告)号：US08425895B2
公开(公告)日：2013-04-23
申请号：US12665682
申请日：2007-06-22
申请人： Haitao Wang , Chunsheng Mao , Jizhi Li , Ling Wang , Yong Du , Longbin Liu , Jing Xu , Rui Zhang
发明人： Haitao Wang , Chunsheng Mao , Jizhi Li , Ling Wang , Yong Du , Longbin Liu , Jing Xu , Rui Zhang
IPC分类号： A61K38/21 , C07K14/00 , C08H1/02 , C12P21/04 , C07H21/04 , A61K38/00
CPC分类号： C07K14/56 , A61K38/00 , C07K14/555
摘要： This application relates to recombinant human interferon-like proteins. In one embodiment a recombinant protein created by gene shuffling technology is described having enhanced anti-viral and anti-proliferative activities in comparison to naturally occurring human interferon alpha 2b (HuIFN-α2b). The invention encompasses a polynucleotide encoding the protein and recombinant vectors and host cells comprising the polynucleotide. Preferably the polynucleotide is selected from the group of polynucleotides each having a sequence at least 93% identical to SEQ ID: No. 1 and the protein is selected from the group of proteins each having an amino acid sequence at least 85% identical to SEQ ID NO: 2. The proteins and compositions comprising the proteins can be used for treatment of conditions responsive to interferon therapy, such as viral diseases and cancer.
摘要翻译：本申请涉及重组人类干扰素样蛋白。在一个实施方案中，与天然存在的人干扰素α2b（HuIFN-α2b）相比，描述了通过基因改组技术产生的重组蛋白质具有增强的抗病毒和抗增殖活性。本发明包括编码蛋白质的多核苷酸和包含多核苷酸的重组载体和宿主细胞。优选地，多核苷酸选自各自具有与SEQ ID：No.1至少93％相同的序列的多核苷酸，并且所述蛋白质选自蛋白质组，其各自具有与SEQ ID NO：至少85％相同的氨基酸序列包含蛋白质的蛋白质和组合物可用于治疗对干扰素治疗（例如病毒性疾病和癌症）有反应的病症。

6. 发明授权

US07625564B2 Recombinant human EPO-Fc fusion proteins with prolonged half-life and enhanced erythropoietic activity in vivo 有权
标题翻译：重组人EPO-Fc融合蛋白具有延长的半衰期和增强体内红细胞生成活性
公开(公告)号：US07625564B2
公开(公告)日：2009-12-01
申请号：US11340661
申请日：2006-01-27
申请人： Haitao Wang , Yong Du , Rui Zhang , Jing Xu , Longbin Liu
发明人： Haitao Wang , Yong Du , Rui Zhang , Jing Xu , Longbin Liu
IPC分类号： A61K39/00 , C07K14/00 , C07K17/00
CPC分类号： C07K14/505 , A61K9/0019 , A61K38/00 , A61K47/6811 , C07K2317/53 , C07K2319/30
摘要： A recombinant fusion protein comprising a human erythropoietin peptide portion linked to an immunoglobulin peptide portion is described. The fusion protein has a prolonged half-life in vivo in comparison to naturally occurring or recombinant native human erythropoietin. In one embodiment of the invention, the protein has a half-life in vivo at least three fold higher than native human erythropoietin. The fusion protein also exhibits enhanced erythropoietic bioactivity in comparison to native human erythropoietin. In one embodiment, the fusion protein comprises the complete peptide sequence of a human erythropoietin (EPO) molecule and the peptide sequence of an Fc fragment of human immunoglobulin IgG1. The Fc fragment in the fusion protein includes the hinge region, CH2 and CH3 domains of human immunoglobulin IgG1. The EPO molecule may be linked directly to the Fc fragment to avoid extraneous peptide linkers and lessen the risk of an immunogenic response when administered in vivo. In one embodiment the hinge region is a human Fc fragment variant having a non-cysteine residue at amino acid 6. The invention also relates to nucleic acid and amino acid sequences encoding the fusion protein and transfected cell lines and methods for producing the fusion protein. The invention further includes pharmaceutical compositions comprising the fusion protein and methods of using the fusion protein and/or the pharmaceutical compositions, for example to stimulate erythropoiesis in subjects in need of therapy.
摘要翻译：描述了包含与免疫球蛋白肽部分连接的人促红细胞生成素肽部分的重组融合蛋白。与天然存在的或重组的天然人促红细胞生成素相比，融合蛋白在体内具有延长的半衰期。在本发明的一个实施方案中，蛋白质的体内半衰期比天然人促红细胞生成素高至少三倍。与天然人促红细胞生成素相比，融合蛋白也表现出增强的红细胞生物活性。在一个实施方案中，融合蛋白包含人促红细胞生成素（EPO）分子的完整肽序列和人免疫球蛋白IgG1的Fc片段的肽序列。融合蛋白中的Fc片段包括人免疫球蛋白IgG1的铰链区，CH2和CH3结构域。 EPO分子可以直接连接到Fc片段，以避免外来的肽接头并且当在体内施用时减轻免疫原性应答的风险。在一个实施方案中，铰链区是在氨基酸6具有非半胱氨酸残基的人Fc片段变体。本发明还涉及编码融合蛋白和转染的细胞系的核酸和氨基酸序列以及用于产生融合蛋白的方法。本发明还包括包含融合蛋白的药物组合物和使用融合蛋白和/或药物组合物的方法，例如刺激需要治疗的受试者的红细胞生成。

7. 发明授权

US07625555B2 Recombinant human interferon-like proteins 有权
标题翻译：重组人干扰素样蛋白
公开(公告)号：US07625555B2
公开(公告)日：2009-12-01
申请号：US11764786
申请日：2007-06-18
申请人： Haitao Wang , Chunsheng Mao , Jizhi Li , Jing Xu , Rui Zhang , Ling Wang , Yong Du , Longbin Liu
发明人： Haitao Wang , Chunsheng Mao , Jizhi Li , Jing Xu , Rui Zhang , Ling Wang , Yong Du , Longbin Liu
IPC分类号： A61K38/21 , C07K14/00 , C08H1/02 , C12P21/04 , C07H21/04 , A61K38/00
CPC分类号： C07K14/56 , A61K38/00 , C07K14/555
摘要： This application relates to recombinant human interferon-like proteins. In one embodiment a recombinant protein created by gene shuffling technology is described having enhanced anti-viral and anti-proliferative activities in comparison to naturally occurring human interferon alpha 2b (HuIFN-α2b). The invention encompasses a polynucleotide encoding the protein and recombinant vectors and host cells comprising the polynucleotide. Preferably the polynucleotide is selected from the group of polynucleotides each having a sequence at least 93% identical to SEQ ID: No. 1 and the protein is selected from the group of proteins each having an amino acid sequence at least 85% identical to SEQ ID No: 2. The proteins and compositions comprising the proteins can be used for treatment of conditions responsive to interferon therapy, such as viral diseases and cancer.
摘要翻译：本申请涉及重组人类干扰素样蛋白。在一个实施方案中，与天然存在的人干扰素α2b（HuIFN-α2b）相比，描述了通过基因改组技术产生的重组蛋白质具有增强的抗病毒和抗增殖活性。本发明包括编码蛋白质的多核苷酸和包含多核苷酸的重组载体和宿主细胞。优选地，多核苷酸选自各自具有与SEQ ID：No.1至少93％相同的序列的多核苷酸，并且所述蛋白质选自蛋白质组，其各自具有与SEQ ID NO：至少85％相同的氨基酸序列否：2.包含蛋白质的蛋白质和组合物可用于治疗对干扰素治疗（例如病毒性疾病和癌症）有反应的病症。

8. 发明授权

US11028398B2 Fusion proteins having mutated immunoglobulin hinge region 有权
公开(公告)号：US11028398B2
公开(公告)日：2021-06-08
申请号：US13275205
申请日：2011-10-17
申请人： Haitao Wang , Yong Du , Rui Zhang , Jing Xu , Longbin Liu
发明人： Haitao Wang , Yong Du , Rui Zhang , Jing Xu , Longbin Liu
IPC分类号： C07K1/00 , A61K45/00 , A61K39/00 , C12N15/62 , A61K47/68 , C07K16/00
摘要： A fusion protein having a non-immunoglobulin polypeptide having a cysteine residue proximal to the C terminal thereof, and an immunoglobulin component with a mutated hinge region is provided. The mutation comprises a point mutated site corresponding in position to the position in a native hinge region of the cysteine residue located nearest the cysteine residue of the non-Ig component. The distance from the cysteine residue of the non-immunoglobulin polypeptide and any remaining cysteine residues of the mutated hinge region is sufficient to prevent the formation of a disulphide bond therebetween.

9. 发明授权

US07867482B2 Recombinant human interferon-like proteins 有权
公开(公告)号：US07867482B2
公开(公告)日：2011-01-11
申请号：US12555759
申请日：2009-09-08
申请人： Haitao Wang , Chungsheng Mao , Jizhi Li , Jing Xu , Rui Zhang , Ling Wang , Yong Du , Longbin Liu
发明人： Haitao Wang , Chungsheng Mao , Jizhi Li , Jing Xu , Rui Zhang , Ling Wang , Yong Du , Longbin Liu
IPC分类号： A61K38/21 , C07K14/00 , C12P21/04 , C07H21/04 , A61K38/00
CPC分类号： C07K14/56 , A61K38/00 , C07K14/555
摘要： This application relates to recombinant human interferon-like proteins. In one embodiment a recombinant protein created by gene shuffling technology is described having enhanced anti-viral and anti-proliferative activities in comparison to naturally occurring human interferon alpha 2b (HuIFN-α2b). The invention encompasses a polynucleotide encoding the protein and recombinant vectors and host cells comprising the polynucleotide. Preferably the polynucleotide is selected from the group of polynucleotides each having a sequence at least 93% identical to SEQ ID: No. 1 and the protein is selected from the group of proteins each having an amino acid sequence at least 85% identical to SEQ ID No: 2. The proteins and compositions comprising the proteins can be used for treatment of conditions responsive to interferon therapy, such as viral diseases and cancer.

10. 发明申请

US20070178112A1 Recombinant human EPO-Fc fusion proteins with prolonged half-life and enhanced erythropoietic activity in vivo 有权
标题翻译：重组人EPO-Fc融合蛋白具有延长的半衰期和增强体内红细胞生成活性
公开(公告)号：US20070178112A1
公开(公告)日：2007-08-02
申请号：US11340661
申请日：2006-01-27
申请人： Haitao Wang , Yong Du , Rui Zhang , Jing Xu , Longbin Liu
发明人： Haitao Wang , Yong Du , Rui Zhang , Jing Xu , Longbin Liu
IPC分类号： A61K39/395 , C12P21/06 , C07K16/46 , C07H21/04 , C12N5/06
CPC分类号： C07K14/505 , A61K9/0019 , A61K38/00 , A61K47/6811 , C07K2317/53 , C07K2319/30
摘要： A recombinant fusion protein comprising a human erythropoietin peptide portion linked to an immunoglobulin peptide portion is described. The fusion protein has a prolonged half-life in vivo in comparison to naturally occurring or recombinant native human erythropoietin. In one embodiment of the invention, the protein has a half-life in vivo at least three fold higher than native human erythropoietin. The fusion protein also exhibits enhanced erythropoietic bioactivity in comparison to native human erythropoietin. In one embodiment, the fusion protein comprises the complete peptide sequence of a human erythropoietin (EPO) molecule and the peptide sequence of an Fc fragment of human immunoglobulin IgG1. The Fc fragment in the fusion protein includes the hinge region, CH2 and CH3 domains of human immunoglobulin IgG1. The EPO molecule may be linked directly to the Fc fragment to avoid extraneous peptide linkers and lessen the risk of an immunogenic response when administered in vivo. In one embodiment the hinge region is a human Fc fragment variant having a non-cysteine residue at amino acid 6. The invention also relates to nucleic acid and amino acid sequences encoding the fusion protein and transfected cell lines and methods for producing the fusion protein. The invention further includes pharmaceutical compositions comprising the fusion protein and methods of using the fusion protein and/or the pharmaceutical compositions, for example to stimulate erythropoiesis in subjects in need of therapy.
摘要翻译：描述了包含与免疫球蛋白肽部分连接的人促红细胞生成素肽部分的重组融合蛋白。与天然存在的或重组的天然人促红细胞生成素相比，融合蛋白在体内具有延长的半衰期。在本发明的一个实施方案中，蛋白质的体内半衰期比天然人促红细胞生成素高至少三倍。与天然人促红细胞生成素相比，融合蛋白也表现出增强的红细胞生物活性。在一个实施方案中，融合蛋白包含人促红细胞生成素（EPO）分子的完整肽序列和人免疫球蛋白IgG1的Fc片段的肽序列。融合蛋白中的Fc片段包括人免疫球蛋白IgG1的铰链区，CH2和CH3结构域。 EPO分子可以直接连接到Fc片段，以避免外来的肽接头并且当在体内施用时减轻免疫原性应答的风险。在一个实施方案中，铰链区是在氨基酸6具有非半胱氨酸残基的人Fc片段变体。本发明还涉及编码融合蛋白和转染细胞系的核酸和氨基酸序列以及用于产生融合蛋白的方法。本发明还包括包含融合蛋白的药物组合物和使用融合蛋白和/或药物组合物的方法，例如刺激需要治疗的受试者的红细胞生成。

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