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1. 发明专利

JP2009232871A Improved system for multi color real time pcr 有权
标题翻译：改进的多色实时PCR系统
公开(公告)号：JP2009232871A
公开(公告)日：2009-10-15
申请号：JP2009170421
申请日：2009-07-21
申请人： F Hoffmann La Roche Ag , エフ.ホフマン−ラロシュアーゲーF. Hoffmann−La Roche Aktiengesellschaft
发明人： SAGNER GREGOR , BECHLER INGRID , BOLTE JOACHIM , HEINDL DIETER , JOSEL HANS-PETER , GUTEKUNST MARTIN , SEIBL RUDOLF , MUELLER CHRISTOPH
IPC分类号： C12M1/00 , C12N15/09 , C12Q1/68
CPC分类号： C12Q1/6851 , G01N2021/6421 , C12Q2565/101 , C12Q2561/113 , C12Q2537/143
摘要： PROBLEM TO BE SOLVED: To provide an improved system for optimizing multiplex/multi-collar detecting experiments, and at the same time, allowing a flexible design.
SOLUTION: The real time PCR device comprises at least one light source radiating the light toward a reaction container containing a fluorescent compound, an excitation unit placed to receive the light from the reaction container and containing an optical pipe uniformly distributing the light for delivering the light to a bundle of optical fibers, a detecting unit containing at least five independent fluorescence detecting entities, the detecting entities each having central detecting wavelengths differing from each other at least by 25 nm, a plurality, at least five, of optical fiber bundles arranged to receive the uniformly distributed light from the optical pipe and to transmit the light to the fluorescence detecting entities, a means for heating and cooling, and a plurality of reaction containers for containing reaction mixtures, wherein the plurality of the detecting entities can simultaneously detect a maximum fluorescence of at least five different fluorescent compounds, and the excitation unit and the detecting unit locate in separated housings.
COPYRIGHT: (C)2010,JPO&INPIT
摘要翻译：要解决的问题：提供一种改进的系统，用于优化多重/多领检测实验，同时允许灵活的设计。解决方案：实时PCR装置包括至少一个光源，将光朝向包含荧光化合物的反应容器辐射，激励单元被放置成接收来自反应容器的光，并且包含均匀分布光的光管将光传送到一束光纤，检测单元包含至少五个独立的荧光检测实体，所述检测实体各自具有至少25nm彼此不同的中心检测波长，多个至少五个光纤束，其布置成接收来自光管的均匀分布的光并将光透射到荧光检测实体，用于加热和冷却的装置以及用于容纳反应混合物的多个反应容器，其中所述多个检测实体可以同时检测至少五种不同荧光化合物的最大荧光，和t 他的激励单元和检测单元位于分离的外壳中。版权所有（C）2010，JPO＆INPIT

2. 发明专利

JP2004065262A Improved fluorescence resonance energy transfer probe 有权
公开(公告)号：JP2004065262A
公开(公告)日：2004-03-04
申请号：JP2003286973
申请日：2003-08-05
申请人： F Hoffmann La Roche Ag , エフ.ホフマン−ラロシュアーゲーF. Hoffmann−La Roche Aktiengesellschaft
发明人： HEINDL DIETER , JOSEL HANS-PETER DR , SAGNER GREGOR , BECHLER INGRID
IPC分类号： G01N33/53 , C12N15/09 , C12Q1/68 , G01N33/542 , G01N33/566 , G01N33/58
CPC分类号： C12Q1/6818
摘要： PROBLEM TO BE SOLVED: To provide an improved FRET hybridization probe designed so that an absolute signal is not affected by the base composition and sequence of a target nucleic acid. SOLUTION: A FRET hybridization probe pair is a FRET hybridization probe pair comprising a substantially complementary nucleotide sequence substance to the target nucleic acid sequence, a fluorescence substance being either one of a FRET donor substance or a FRET acceptor substance, and a spacer substance connecting the nucleotide substance with the fluorescence substance respectively. The FRET hybridization probe pair is adjacently hybridizable to the target nucleic acid sequence. The spacer substances between the two members of the FRET hybridization probe pair can mutually form non-covalent bonding interaction. COPYRIGHT: (C)2004,JPO

3. 发明专利

JP2006075167A Real time pcr accompanying addition of pyrophosphatase 审中-公开
标题翻译：伴随添加磷酸氢钙酶的实时PCR
公开(公告)号：JP2006075167A
公开(公告)日：2006-03-23
申请号：JP2005261187
申请日：2005-09-08
申请人： F Hoffmann La Roche Ag , エフ.ホフマン−ラロシュアーゲーF. Hoffmann−La Roche Aktiengesellschaft
发明人： BOELL INGA , DEGEN ANJA , HEINDL DIETER , SAGNER GREGOR
IPC分类号： C12N15/09 , C12Q1/68 , G01N21/78
CPC分类号： C12Q1/6818 , C12Q1/6851 , C12Q1/686 , C12Q2531/113 , C12Q2561/113 , C12Q2521/525 , C12Q2565/301
摘要： PROBLEM TO BE SOLVED: To provide an improved performance in melting curve analysis in a quantitative real time PCR amplification and following later-stage amplification cycle. SOLUTION: The composition for amplifying and detecting a target nucleic acid comprises a thermostable DNA polymerase, a mixture of deoxynucleoside triphosphate, a buffer, at least two amplification primers and at least one first hybridization probe labeled with the first fluorescent substance, and further contains pyrophosphatase. COPYRIGHT: (C)2006,JPO&NCIPI
摘要翻译：要解决的问题：在定量实时PCR扩增和后期扩增循环中提供改进的熔解曲线分析性能。解决方案：用于扩增和检测靶核酸的组合物包含热稳定DNA聚合酶，脱氧核苷三磷酸盐，缓冲液，至少两个扩增引物和至少一个用第一荧光物质标记的第一杂交探针的混合物，以及还含有焦磷酸酶。版权所有（C）2006，JPO＆NCIPI

4. 发明专利

JP2004121252A Improved fret method 审中-公开
公开(公告)号：JP2004121252A
公开(公告)日：2004-04-22
申请号：JP2003344928
申请日：2003-10-02
申请人： F Hoffmann La Roche Ag , エフ.ホフマン−ラロシュアーゲーF. Hoffmann−La Roche Aktiengesellschaft
发明人： SAGNER GREGOR , HEINDL DIETER , BECHLER INGRID , KRAUSE CHRISTINA
IPC分类号： G01N33/53 , C12N15/09 , C12Q1/68 , G01N33/566
CPC分类号： C12Q1/6818
摘要： PROBLEM TO BE SOLVED: To provide an improved hybridization probe pair and to provide a technology using the same. SOLUTION: A pair of FRET hybridization probes adjacently hybridizing to a target nucleic acid sequence, a first member of the pair of hybridization probes comprising (i) a base sequence which is substantially complementary to the sequence of the target nucleic acid, (ii) a fluorescent entity, said entity being either the FRET donor entity or the FRET acceptor entity and (iii) a spacer entity connecting the base sequence entity and the fluorescent entity, said spacer entity comprising a connecting chain of at least 15 atoms. The pair of FRET hybridization probes which have 2 atoms of the connecting chain comprising at least 15 atoms having negatively charged substituents; a composition or a kit containing the same; a method for qualitatively or quantitatively detecting the target nucleic acid sequence in a sample by using the same; method for the determination of the melting profile of a hybrid consisting of a target nucleic acid and a pair of FRET hybridization probes; method for chemical solid phase synthesis of multiple oligonucleotides. COPYRIGHT: (C)2004,JPO

5. 发明专利

JP2004049241A Fluoresce hybridization probe lowering background 有权
公开(公告)号：JP2004049241A
公开(公告)日：2004-02-19
申请号：JP2003278482
申请日：2003-07-23
申请人： F Hoffmann La Roche Ag , エフ.ホフマン−ラロシュアーゲーF. Hoffmann−La Roche Aktiengesellschaft
发明人： HEINDL DIETER , JOSEL HANS-PETER DR , SAGNER GREGOR , MAYR JUTTA
IPC分类号： G01N33/53 , C12N15/09 , C12Q1/68 , G01N33/566 , G01N33/58 , G01N37/00
CPC分类号： C12Q1/6818
摘要： PROBLEM TO BE SOLVED: To provide an FRET hybridization probe giving improved signal strength and considerably wide dynamic range independently of type of applied identification pigments.
SOLUTION: The FRET hybridization probe pair comprises the first oligonucleotide having an FRET donor substance and the second oligonucleotide having an FRET acceptor substance, wherein an oligonucleotide having a donor fluorescent substance has at least one second substance and the second substance is a compound extinguishing fluorescene of the donor fluorescent substance.
COPYRIGHT: (C)2004,JPO

6. 发明申请

WO2004087950A3 IMPROVED SYSTEM FOR MULTI COLOR REAL TIME PCR 审中-公开
标题翻译：改进的多色实时PCR系统
公开(公告)号：WO2004087950A3
公开(公告)日：2004-11-25
申请号：PCT/EP2004003457
申请日：2004-04-01
申请人： ROCHE DIAGNOSTICS GMBH , HOFFMANN LA ROCHE , SAGNER GREGOR , BECHLER INGRID , BOLTE JOACHIM , HEINDL DIETER , JOSEL HANS-PETER , GUTEKUNST MARTIN , SEIBL RUDOLF , MUELLER CHRISTOPH
发明人： SAGNER GREGOR , BECHLER INGRID , BOLTE JOACHIM , HEINDL DIETER , JOSEL HANS-PETER , GUTEKUNST MARTIN , SEIBL RUDOLF , MUELLER CHRISTOPH
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6851 , G01N2021/6421 , C12Q2565/101 , C12Q2561/113 , C12Q2537/143
摘要： The invention is directed to a system for performing multi-color real time PCT, comprising a flexible real time PCT instrument and a specific composition or reaction mixture for performing multiplex PCR: In particular, the present invention is directed to a composition or reaction mixtuer which comprises at least 3, preferably 4-5 and most preferably exactly 4 pairs of FRET hybridization probes. Each pair of said hybridization probes consists of a FRET donor probe carrying a FRET donor moiety and a FRET acceptor probe carrying a FRET acceptor moiety having an emission maximum between 550 and 710 nm.
摘要翻译：本发明涉及一种用于执行多色实时PCT的系统，其包括灵活的实时PCT仪器和用于进行多重PCR的特定组合物或反应混合物：具体地，本发明涉及组合物或反应混合物，其中包含至少3个，优选4-5个，最优选恰好4对FRET杂交探针。每对所述杂交探针由携带FRET供体部分的FRET供体探针和携带具有550-710nm发射最大值的FRET受体部分的FRET受体探针组成。

7. 发明专利

CA2699011A1 FLUID TRANSFER CONTROL 未知
公开(公告)号：CA2699011A1
公开(公告)日：2010-10-09
申请号：CA2699011
申请日：2010-04-06
申请人： HOFFMANN LA ROCHE
发明人： HORAT MARTIN , SAGNER GREGOR
IPC分类号： G01N21/11 , C12M1/34 , C12M1/38 , C12Q1/68 , G01N33/52 , G01N33/58 , G01N35/10 , G01T1/167 , G01T1/20
摘要： The present invention provides a fluid transfer control method, said method is based on measurements of intensities of dyes within the fluid to be transferred. In more detail, the present invention makes use of control dyes and quencher molecules for the fluid transfer controls.

8. 发明专利

CA2525482A1 IMPROVED SYSTEM FOR MULTI COLOR REAL TIME PCR 未知
公开(公告)号：CA2525482A1
公开(公告)日：2004-10-14
申请号：CA2525482
申请日：2004-04-01
申请人： HOFFMANN LA ROCHE
发明人： BECHLER INGRID , SAGNER GREGOR , MUELLER CHRISTOPH , BOLTE JOACHIM , SEIBL RUDOLF , GUTEKUNST MARTIN , JOSEL HANS-PETER , HEINDL DIETER
IPC分类号： C12Q1/68
摘要： The invention is directed to a system for performing multi-color real time PCT, comprising a flexible real time PCT instrument and a specific compositi on or reaction mixture for performing multiplex PCR: In particular, the present invention is directed to a composition or reaction mixtuer which comprises a t least 3, preferably 4-5 and most preferably exactly 4 pairs of FRET hybridization probes. Each pair of said hybridization probes consists of a FRET donor probe carrying a FRET donor moiety and a FRET acceptor probe carrying a FRET acceptor moiety having an emission maximum between 550 and 7 10 nm.

9. 发明专利

CA2525482C IMPROVED SYSTEM FOR MULTICOLOR REAL-TIME PCR COMPRISING 3 TO 6 PAIRS OF FORESTER RESONANCE ENERGY TRANSFER (FRET) PROBES 未知
公开(公告)号：CA2525482C
公开(公告)日：2015-02-17
申请号：CA2525482
申请日：2004-04-01
申请人： HOFFMANN LA ROCHE
发明人： SAGNER GREGOR , BECHLER INGRID , BOLTE JOCHEN , HEINDL DIETER , JOSEL HANS-PETER , GUTEKUNST MARTIN , SEIBL RUDOLF , MUELLER CHRISTOPH
IPC分类号： C12Q1/68
摘要： The invention is directed to a system for performing multi-color real time PCT, comprising a flexible real time PCT instrument and a specific composition or reaction mixture for performing multiplex PCR: In particular, the present invention is directed to a composition or reaction mixtuer which comprises at least 3, preferably 4-5 and most preferably exactly 4 pairs of FRET hybridization probes. Each pair of said hybridization probes consists of a FRET donor probe carrying a FRET donor moiety and a FRET acceptor probe carrying a FRET acceptor moiety having an emission maximum between 550 and 710 nm.

10. 发明专利

CA2896616A1 IMPROVED CALIBRATION OF HIGH RESOLUTION MELTING 未知
公开(公告)号：CA2896616A1
公开(公告)日：2014-07-17
申请号：CA2896616
申请日：2014-01-08
申请人： HOFFMANN LA ROCHE
发明人： REISER ASTRID , SAGNER GREGOR
IPC分类号： C12Q1/68
摘要： The present invention refers to a method and a kit for performing temperature calibration in high resolution melting PCR experiments. The present invention further refers to a method for optimal calibration allowing read-out of identical or similar melting temperatures for target and calibrator. The present invention further refers to an apparatus for performing the method and a computer program for executing the method.

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