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1. 发明专利

JP2008157939A METHOD FOR DETECTION OF cAMP AND cGMP 有权
标题翻译：检测cAMP和cGMP的方法
公开(公告)号：JP2008157939A
公开(公告)日：2008-07-10
申请号：JP2007326193
申请日：2007-12-18
申请人： F Hoffmann La Roche Ag , エフ.ホフマン−ラロシュアーゲーF. Hoffmann−La Roche Aktiengesellschaft
发明人： ENDERLE THILO , MATILE HUGUES , ROTH DORIS
IPC分类号： G01N33/542 , G01N21/78 , G01N33/53
CPC分类号： G01N33/542 , C12Q1/68 , G01N33/5735
摘要： PROBLEM TO BE SOLVED: To provide a simple assay for detecting cAMP (cyclic adenosine monophosphate formed upon modulation of adenylyl cyclase activity), and an in vitro method for indirectly detecting receptor activity and for screening of ligands. SOLUTION: The in vitro method for detecting cAMP or cGMP comprises: (a) contacting a mixture with a complex of a tracer and a dequencher, wherein the tracer is a fluorophore covalently linked to cAMP quencher; and (b) measuring the change in fluorescence. Furthermore, the method is used for determining the cAMP or cGMP concentration in the mixture, for determining the activity of a receptor wherein the signal transduction of the receptor comprises cAMP or cGMP, and for screening of a ligand for such a receptor. COPYRIGHT: (C)2008,JPO&INPIT
摘要翻译：要解决的问题：提供用于检测cAMP（调节腺苷酸环化酶活性时形成的环状腺苷一磷酸）的简单测定法，以及间接检测受体活性和筛选配体的体外方法。解决方案：用于检测cAMP或cGMP的体外方法包括：（a）使混合物与示踪剂和去屑剂的复合物接触，其中示踪剂是与cAMP猝灭剂共价连接的荧光团; 和（b）测量荧光的变化。此外，该方法用于确定混合物中的cAMP或cGMP浓度，用于确定受体的活性，其中受体的信号转导包含cAMP或cGMP，并用于筛选这种受体的配体。版权所有（C）2008，JPO＆INPIT

2. 发明专利

JP2007037546A Generic kinase assay 有权
标题翻译：一般激酶测定
公开(公告)号：JP2007037546A
公开(公告)日：2007-02-15
申请号：JP2006207285
申请日：2006-07-31
申请人： F Hoffmann La Roche Ag , エフ.ホフマン−ラロシュアーゲーF. Hoffmann−La Roche Aktiengesellschaft
发明人： ENDERLE THILO , ROTH DORIS
IPC分类号： C12Q1/42 , C12Q1/48 , G01N33/542
CPC分类号： C12Q1/485 , C12Q1/42 , G01N33/542
摘要： PROBLEM TO BE SOLVED: To provide an effective method of determining kinase or phosphatase activity based on measuring energy transfer between an energy donor and an energy acceptor.
SOLUTION: The invention relates to the method of determining kinase or phosphatase activity based on a three parts system. The method comprises contacting a binding partner (a metal ion on a bead) which can bind phosphorylated peptides, a detection molecule and a substrate peptide. Determination of activities is achieved by measuring energy transfer between an energy donor (Ru-luminophore) and an energy acceptor (phosphor) that are present on the detection molecule and the substrate molecule.
COPYRIGHT: (C)2007,JPO&INPIT
摘要翻译：要解决的问题：提供基于测量能量供体和能量受体之间的能量转移来确定激酶或磷酸酶活性的有效方法。解决方案：本发明涉及基于三部分系统确定激酶或磷酸酶活性的方法。该方法包括使可结合磷酸化肽的结合配偶体（珠粒上的金属离子），检测分子和底物肽接触。通过测量存在于检测分子和底物分子上的能量供体（Ru-发光体）和能量受体（荧光体）之间的能量转移来实现活性的测定。版权所有（C）2007，JPO＆INPIT

3. 发明专利

JP2006115843A Assay for tracing ligand receptor 审中-公开
标题翻译：追踪配体受体的测定
公开(公告)号：JP2006115843A
公开(公告)日：2006-05-11
申请号：JP2005308002
申请日：2005-10-24
申请人： F Hoffmann La Roche Ag , エフ.ホフマン−ラロシュアーゲーF. Hoffmann−La Roche Aktiengesellschaft
发明人： APFEL CHRISTIAN , ENDERLE THILO , ZOFFMANN SANNAH J , MOSER MIREILLE
IPC分类号： C12Q1/02 , C12N15/09 , G01N33/15 , G01N33/50 , G01N33/53 , G01N33/543
CPC分类号： G01N33/582
摘要： PROBLEM TO BE SOLVED: To provide a method for measuring the effects of many cell groups to many cell parameters to enable the screening of a compound, and to provide an assay for tracing a ligand receptor.
SOLUTION: An image-based method comprises screening a compound on the basis of the measurement of the internalization of a fluorescent light labeled ligand. A multiplication method for screening the high content of a compound comprises measuring at least one cell parameter.
COPYRIGHT: (C)2006,JPO&NCIPI
摘要翻译：要解决的问题：提供一种用于测量许多细胞组对许多细胞参数的影响以使得能够筛选化合物并提供用于追踪配体受体的测定法的方法。解决方案：基于图像的方法包括基于荧光灯标记的配体的内化测量来筛选化合物。用于筛选化合物的高含量的增殖方法包括测量至少一个细胞参数。版权所有（C）2006，JPO＆NCIPI

4. 发明专利

JP2005120093A Fp-ghrelin 有权
标题翻译： FP-GHRELIN
公开(公告)号：JP2005120093A
公开(公告)日：2005-05-12
申请号：JP2004302925
申请日：2004-10-18
申请人： F Hoffmann La Roche Ag , エフ.ホフマン−ラロシュアーゲーF. Hoffmann−La Roche Aktiengesellschaft
发明人： ENDERLE THILO , GRAF MARTIN , HERTEL CORNELIA , ZOFFMANN SANNAH J , KITAS ERIC A
IPC分类号： G01N33/53 , A61K45/00 , A61P5/06 , A61P43/00 , C07K7/08 , C07K14/60 , G01N31/22 , G01N33/566 , G01N33/58
CPC分类号： C07K14/60 , G01N31/22
摘要： PROBLEM TO BE SOLVED: To provide a fluorescent-labeled ghrelin analog for high-throughput screening.
SOLUTION: The fluorescent-labeled growth hormone secretogogue can be used for identification of a compound to bond to a receptor of the growth hormone secretogogue especially for identification by high-throughput screening.
COPYRIGHT: (C)2005,JPO&NCIPI
摘要翻译：要解决的问题：提供荧光标记的生长素释放肽类似物进行高通量筛选。解决方案：荧光标记的生长激素分泌途径可用于鉴定与生长激素分泌的受体结合的化合物，特别是通过高通量筛选来鉴定。版权所有（C）2005，JPO＆NCIPI

5. 发明专利

JP2001194375A MICRO AMOUNT DISPENSING SYSTEM TO IMPARTING LIQUID IN OPEN JET 失效
公开(公告)号：JP2001194375A
公开(公告)日：2001-07-19
申请号：JP2000340596
申请日：2000-11-08
申请人： HOFFMANN LA ROCHE
发明人： ENDERLE THILO , FATTINGER CHRISTOF , FLURY GERHARD , HIRT HANS-PETER , RIETMANN RENE , TSCHIRKY HANSJOERG
IPC分类号： G01N35/10 , B01L3/02 , B05B1/14 , B05B1/30 , F16K1/00 , F16K7/12 , F16K31/06
摘要： PROBLEM TO BE SOLVED: To provide a dispensing system which gives a micro amount of a liquid having extensively diversified physical and chemical characteristics to a target, causes no pollution, suppresses losses to a minimum, estimates the quantity to be given in advance, and is not substantially affected by the shearing force, the temperature rise and the pressure wave, sturdy and inert. SOLUTION: This dispensing system comprises a valve chamber 16 which is demarcated by a non-permeable elastic film 11 and filled with a liquid, and at least one liquid feed lines 24, 26 communicated with the valve chamber 16, and has a valve in which an outlet closed from the outside by the film at the rest position is communicated with an imparting nozzle 10 from the chamber, and imparts the liquid in an open jet. An actuator chamber 29 is disposed opposite to the film, an electro-mechanical drive 30 is provided in the actuator chamber 29 to temporarily lift the film from the outlet, and connected to the film, and closed in the pressure-sealed condition so that the actuator chamber can apply the pressure different from the atmospheric pressure.

6. 发明专利

ES2368669T3 ENSAYO GENERICO DE DETECCION DE ACTIVIDAD QUINASA/FOSFATASA CON UNA UNICA LECTURA. 未知
公开(公告)号：ES2368669T3
公开(公告)日：2011-11-21
申请号：ES08801776
申请日：2008-09-01
申请人： HOFFMANN LA ROCHE
发明人： ENDERLE THILO , ROTH DORIS
IPC分类号： C12Q1/42 , C12Q1/48 , G01N33/542
摘要： Un método para detectar una actividad quinasa o actividad fosfatasa que comprende los pasos: a) incubar una muestra con actividad quinasa o fosfatasa con una molécula sustrato quinasa o fosfatasa que comprende un fluoróforo con un marcaje detectable, b) incubar la mezcla del paso a) con una entidad de detección que comprende un grupo aromático que se selecciona de entre el grupo que consiste en los aminoácidos triptófano, tirosina y fenilalanina, y una pareja de unión, en la que ambas molécula de sustrato fosforilado y la entidad de detección son capaces de unirse a la pareja de unión y la unión de la molécula sustrato y la entidad de detección a la pareja de unión conduce a una lectura alterada del fluoróforo, en el que la lectura alterada del fluoróforo se debe al bloqueo estático o dinámico que resulta de la interacción directa de orbitales moleculares del fluoróforo de la molécula sustrato y el grupo aromático de la entidad de detección, y c) medir el marcaje del fluoróforo en la mezcla del paso b), en el que una lectura alterada del fluoróforo en comparación con el blanco es indicativo de la presencia de una actividad quinasa o fosfatasa en la muestra.

7. 发明专利

DE602004012207D1 未知
公开(公告)号：DE602004012207D1
公开(公告)日：2008-04-17
申请号：DE602004012207
申请日：2004-10-08
申请人： HOFFMANN LA ROCHE
发明人： ENDERLE THILO , GRAF MARTIN , HERTEL CORNELIA , ZOFFMANN SANNAH JENSEN , KITAS ERIC ARGIRIOS
IPC分类号： C07K14/60 , A61K45/00 , G01N33/53 , A61P5/06 , A61P43/00 , C07K7/08 , G01N31/22 , G01N33/566 , G01N33/58 , G01N33/74
摘要： The present invention relates to a fluorescently labeled growth hormone secretagogue which can be used for the identification of compounds capable of binding to growth hormone secretagogue receptors, in particular by high throughput screening.

8. 发明专利

CA2552986A1 GENERIC KINASE ASSAY 未知
公开(公告)号：CA2552986A1
公开(公告)日：2007-01-29
申请号：CA2552986
申请日：2006-07-20
申请人： HOFFMANN LA ROCHE
发明人： ENDERLE THILO , ROTH DORIS
IPC分类号： C12Q1/48 , C12Q1/42 , G01N33/573 , G01N33/58
摘要： The present invention relates to a method of determining kinase or phosphata se activity based on a three parts system. The method comprises contacting a binding partner which can bind phosphorylated peptides, a detection molecule and a substrate peptide. Determination of activities is achieved by measuring energy transfe r between an energy donor and an energy acceptor that are present on the detection molecu le and the substrate molecule.

9. 发明专利

AU5417199A Device for transporting and handling microtiter plates 未知
公开(公告)号：AU5417199A
公开(公告)日：2000-02-28
申请号：AU5417199
申请日：1999-07-30
申请人： ZEISS CARL JENA GMBH , HOFFMANN LA ROCHE
发明人： AMELING RICHARD , ENDERLE THILO , FATTINGER CHRISTOF , GLUCH MARTIN , TSCHIRKY HANSJORG
IPC分类号： G01N31/20 , B65G43/00 , C12M1/00 , C40B60/14 , G01N35/00 , G01N35/02 , G01N35/04 , G01N37/00 , B01J19/00
摘要： A transport system for transporting and handling microtiter plates, for use in high throughput screening, diagnosis and/or combinatorial chemistry, comprising modules with devices for preparing specimens and/or introducing specimens, and/or for optical readout and/or for plate storage and/or devices for further processing steps or readout steps. The system includes an inter-modular transport system for transporting the microtiter plates between the different devices and at least one central transport system for asynchronous plate transfer between individual modules via input and output buffers.

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