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1. 发明专利

JP2011125337A Method for detection of rna molecule, kit and use related thereto 审中-公开
标题翻译：检测RNA分子，包装及其使用方法
公开(公告)号：JP2011125337A
公开(公告)日：2011-06-30
申请号：JP2010280527
申请日：2010-12-16
申请人： F Hoffmann La Roche Ag , エフ.ホフマン−ラロシュアーゲーF. Hoffmann−La Roche Aktiengesellschaft
发明人： ALBERS ANDREAS , ANKENBAUER WALTRAUD , BERGMANN FRANK , FROEHNER STEFANIE , HEINDL DIETER
IPC分类号： C12Q1/68 , C12N15/09 , C12N15/113
CPC分类号： C12Q1/686 , C12Q2525/207 , C12Q2525/186 , C12Q2525/155
摘要： PROBLEM TO BE SOLVED: To provide an improved detection method for a low RNA molecule.
SOLUTION: The method for the detection of an RNA molecule comprises: (a) a step of providing a sample containing the RNA molecule; (b) a step of hybridizing a first polynucleotide to the RNA molecule; (c) a step of reversely transcribing the RNA molecule to generate a first strand cDNA; (d) a step of hybridizing a second polynucleotide to the first strand cDNA, wherein the second polynucleotide is 3'-inextensible oligonucleotide containing (i) 3'-moiety complementary to a part of the first strand cDNA and (ii) 5'-projection containing the sequence of a second primer binding site; (e) a step of obtaining an extension reaction product containing a sequence complementary to the second primer binding site from the first strand cDNA; (f) a step of amplifying the extension reaction product by using the second primer complementary to the first primer and second primer binding sites and by means of polymerase chain reaction; and (g) a step of detecting the amplification product by means of real-time fluorescence readout.
COPYRIGHT: (C)2011,JPO&INPIT
摘要翻译：要解决的问题：为低RNA分子提供改进的检测方法。解决方案：用于检测RNA分子的方法包括：（a）提供含有RNA分子的样品的步骤; （b）将第一多核苷酸与RNA分子杂交的步骤; （c）逆转录RNA分子以产生第一链cDNA的步骤; （d）将第二多核苷酸与第一链cDNA杂交的步骤，其中第二个多核苷酸是3'不可扩展的寡核苷酸，其含有（i）与第一链cDNA的一部分互补的3'-部分，和（ii）包含第二引物结合位点序列的突出物; （e）从第一链cDNA获得含有与第二引物结合位点互补的序列的延伸反应产物的步骤; （f）通过使用与第一引物和第二引物结合位点互补的第二引物并通过聚合酶链反应来扩增延伸反应产物的步骤; 和（g）通过实时荧光读数检测扩增产物的步骤。版权所有（C）2011，JPO＆INPIT

2. 发明专利

JP2011088892A New fluorescence quencher molecule, and method for utilizing the same and use thereof 有权
标题翻译：新型荧光探针分子及其利用方法及其应用
公开(公告)号：JP2011088892A
公开(公告)日：2011-05-06
申请号：JP2010234727
申请日：2010-10-19
申请人： F Hoffmann La Roche Ag , エフ.ホフマン−ラロシュアーゲーF. Hoffmann−La Roche Aktiengesellschaft
发明人： BECKERT RAINER , BERGMANN FRANK , HEINDL DIETER , HERRMANN RUPERT , JOSEL HANS-PETER DR , WELZEL THOMAS
IPC分类号： C07D401/04 , C12N15/09 , C12Q1/68 , G01N21/78
CPC分类号： C07D401/04
摘要： PROBLEM TO BE SOLVED: To provide a new quencher preferably having low background signal and/or high quenching efficiency. SOLUTION: The invention relates to: novel pyridinyl-isoquinoline-dione derivatives; methods of producing these derivatives; conjugates comprising novel pyridinyl-isoquinoline-dione derivatives and (i) a solid support, or (ii) a biomolecule; methods of producing these conjugates; and the use of these conjugates as quenchers in fluorescence resonance energy transfer (FRET). COPYRIGHT: (C)2011,JPO&INPIT
摘要翻译：要解决的问题：提供优选具有低背景信号和/或高猝熄效率的新的猝灭剂。解决方案：本发明涉及：新的吡啶基 - 异喹啉二酮衍生物; 生产这些衍生物的方法; 包含新的吡啶基 - 异喹啉二酮衍生物和（i）固体支持物或（ii）生物分子的缀合物; 生产这些缀合物的方法; 以及这些共轭物在荧光共振能量转移（FRET）中的猝灭剂的使用。版权所有（C）2011，JPO＆INPIT

3. 发明专利

JP2008113669A N8- and c8-linked purine base and structurally related heterocycle as universal nucleoside used for oligonucleotide hybridization 有权
标题翻译： N8-和C8-连接的碱基和结构相关的杂交体作为用于寡核苷酸杂交的通用核苷酸
公开(公告)号：JP2008113669A
公开(公告)日：2008-05-22
申请号：JP2008014190
申请日：2008-01-24
申请人： F Hoffmann La Roche Ag , エフ.ホフマン−ラロシュアーゲーF. Hoffmann−La Roche Aktiengesellschaft
发明人： SEELA FRANK , DEBELAK HARALD , BERGMANN FRANK , HEINDL DIETER , VON DER ELTZ HERBERT
IPC分类号： C12N15/09 , C12Q1/68 , A61K31/70 , C07H19/04 , C07H19/10 , C07H19/16 , C07H19/20 , C07H19/23 , C07H21/00 , C07H21/04
CPC分类号： C07H19/10 , C07H19/04 , C07H19/20 , C07H21/00
摘要： PROBLEM TO BE SOLVED: To provide a nucleic acid binding compound such as a primer or a probe including universal bases that can form better base pairs together with four natural bases together. SOLUTION: This is a nucleic acid binding compound including a detectable reporter group wherein the nucleic acid binding compound includes a back bone, on which a hetero ring group is attached, and can form base pairs together with natural bases. At least one of the hetero cycle groups is N 8 -glycocilated 8-aza-7-deaza-adenine. COPYRIGHT: (C)2008,JPO&INPIT
摘要翻译：要解决的问题：提供核酸结合化合物，例如引物或探针，包括能与四种天然碱基一起形成更好碱基对的通用碱基。解决方案：这是一种核酸结合化合物，其包括可检测的报告基团，其中核酸结合化合物包括其上附着杂环基团的背骨，并且可以与天然碱一起形成碱基对。至少一个杂循环基团是N-SP 8 - 糖化8-氮杂-7-去氮腺嘌呤。版权所有（C）2008，JPO＆INPIT

4. 发明专利

JP2004203878A Mannitol and glucitol derivative 有权
公开(公告)号：JP2004203878A
公开(公告)日：2004-07-22
申请号：JP2003422631
申请日：2003-12-19
申请人： F Hoffmann La Roche Ag , エフ.ホフマン−ラロシュアーゲーF. Hoffmann−La Roche Aktiengesellschaft
发明人： BERGMANN FRANK , VON DER ELTZ HERBERT , SEIDEL CHRISTOPH DR , WEINDEL KURT DR
IPC分类号： G01N33/53 , C07C31/26 , C07D211/56 , C07D309/14 , C07D335/02 , C07D493/10 , C07F9/547 , C07F9/655 , C07H5/04 , C07H5/06 , C07H21/00 , C12N15/09 , C12P19/34 , C12Q1/68 , G01N21/78 , G01N33/533 , G01N33/566
CPC分类号： C07H21/00
摘要： PROBLEM TO BE SOLVED: To provide a novel compound used for integrating a labelling into nucleic acid. SOLUTION: This invention relates to a compound containing a mannitol moiety or a glucitol moiety, especially a specific compound having a mannitol moiety or a glucitol moiety and usable for forming an oligomer compound. The invention relates to the use of the oligomer compound for hybridization and as a probe. A detection method for a nucleic acid in a sample by using the oligomer compound is also provided. COPYRIGHT: (C)2004,JPO&NCIPI

5. 发明专利

JP2014083053A Method of preventing high molecular weight products during amplification 审中-公开
标题翻译：在放大期间防止高分子量产品的方法
公开(公告)号：JP2014083053A
公开(公告)日：2014-05-12
申请号：JP2013214714
申请日：2013-10-15
申请人： F. Hoffmann-La Roche Ag , エフ.ホフマン−ラロシュアーゲーF. Hoffmann−La Roche Aktiengesellschaft
发明人： REINER BABIEL , BERGMANN FRANK , DOROTHEA SIZMANN
IPC分类号： C12N15/09 , C12Q1/68
CPC分类号： C12Q1/707 , C12Q1/6844 , C12Q1/6848 , C12Q1/6853 , C12Q1/686 , C12Q2525/161 , C12Q2525/173 , C12Q2531/113
摘要： PROBLEM TO BE SOLVED: To provide a method of preventing, or partially or completely inhibiting formation of high molecular weight products during amplification.SOLUTION: The invention relates to an improved method for amplifying and detecting target nucleic acids that may be present in a biological sample, comprising a primer pair for generating an amplicon where at least one primer is modified at the 5' terminus with a poly N sequence that is non-complementary to a target sequence, and a detectable probe specific for the amplicon or a DNA binding dye. The invention also provides use of an appropriately modified primer, and of a kit for preventing or inhibiting formation of high molecular weight products during amplification and for detecting the target nucleic acids, comprising the primer.
摘要翻译：要解决的问题：提供在扩增期间预防或部分或完全抑制高分子量产物形成的方法。解决方案：本发明涉及用于扩增和检测可能存在于生物样品中的靶核酸的改进方法，其包含用于产生扩增子的引物对，其中至少一个引物在5'末端被修饰为与靶序列不互补的多核苷酸序列，以及可检测的针对扩增子或DNA结合染料的探针。本发明还提供适当修饰的引物和用于在扩增期间预防或抑制高分子量产物形成并用于检测包含引物的靶核酸的试剂盒的用途。

6. 发明专利

JP2006055166A Method to reduce false positive result 审中-公开
标题翻译：减少假阳性结果的方法
公开(公告)号：JP2006055166A
公开(公告)日：2006-03-02
申请号：JP2005236516
申请日：2005-08-17
申请人： F Hoffmann La Roche Ag , エフ.ホフマン−ラロシュアーゲーF. Hoffmann−La Roche Aktiengesellschaft
发明人： BERGMANN FRANK , ESCHERICH ACHIM , HEINDL DIETER , KREBS JANE , DER ELTZ HERBERT VON
IPC分类号： C12Q1/68 , C12M1/00 , C12N15/09
CPC分类号： C12Q1/689 , C12Q1/6834 , C12Q2537/159 , C12Q2565/101
摘要： PROBLEM TO BE SOLVED: To provide a method and a compound for detection of biological molecules in a sample that decontaminates reagents, necessary for said detection, from biological molecules and/or maintains said reagents free of biological molecules. SOLUTION: The invention relates to the method for the detection of biological molecules in the sample that avoids the detection of biological molecules potentially present in the reagent necessary to detect the biological molecules in the sample, comprising steps of (a) providing a sample potentially comprising said biological molecule, (b) providing binding moieties coupled to the surface of a solid phase, (c) providing reagents necessary to detect the biological molecule, (d) adding said reagents to said sample, (e) detecting the biological molecules in the sample, wherein in step (c) or in step (d) or in step (c) and (d) said reagents are in physical contact with said binding moieties under conditions, whereby said biological molecules potentially present in said reagents bind to said binding moieties coupled to said surface of a solid phase. COPYRIGHT: (C)2006,JPO&NCIPI
摘要翻译：要解决的问题：提供用于检测生物分子的方法和化合物，其用于从生物分子中除去所需检测所需的试剂和/或维持所述没有生物分子的试剂。解决方案：本发明涉及检测样品中生物分子的方法，其避免检测可能存在于检测样品中的生物分子所必需的试剂中的生物分子，包括以下步骤：（a）提供可能包含所述生物分子的样品，（b）提供与固相表面偶联的结合部分，（c）提供检测生物分子所需的试剂，（d）将所述试剂加入到所述样品中，（e）分子，其中在步骤（c）或步骤（d）或步骤（c）和（d）中，所述试剂在条件下与所述结合部分物理接触，由此可能存在于所述试剂中的所述生物分子结合到与所述固相表面偶联的所述结合部分。版权所有（C）2006，JPO＆NCIPI

7. 发明专利

JP2005118041A Method for isolating nucleic acid 有权
标题翻译：分离核酸的方法
公开(公告)号：JP2005118041A
公开(公告)日：2005-05-12
申请号：JP2004294245
申请日：2004-10-06
申请人： F Hoffmann La Roche Ag , エフ.ホフマン−ラロシュアーゲーF. Hoffmann−La Roche Aktiengesellschaft
发明人： BERGMANN FRANK , KIRCHGESSER MICHAEL , WALTER THOMAS , WEINDEL KURT DR , ZIELENSKI RALF , SAROFIM EMAD
IPC分类号： C12N15/09 , C12N15/10 , C12Q1/68
CPC分类号： C12N15/1006 , C12N15/1003 , C12N15/1013
摘要： PROBLEM TO BE SOLVED: To provide a method for purifying a nucleic acid, by substituting a substance which is used in an aqueous adsorption solution for accelerating bonding of the nucleic acid to a substrate, such as an inorganic support material, with another substance. SOLUTION: This method for purifying the nucleic acid includes processes (a) to (d) and, optionally, a process (e) as follows: a process (a) for making the nucleic acid which is derived from a composition containing (i) an aqueous buffer solution, (ii) a salt of a high concentration, (iii) a water-miscible non-acidic organic compound having a functional group expressed by formula I (W is C or S; and Z is O or N), and (iv) the nucleic acid adsorbed on the substrate; (b) a process for, optionally, washing the substrate containing the adsorbed nucleic acid by a washing solution; (c) a process for making the nucleic acid desorbed from the substrate, by contacting the substrate containing the adsorbed nucleic acid with a solution containing the salt of which the concentration is lower when compared with the composition in the process (a); (d) a process for purifying the nucleic acid, by separating a solution containing the desorbed nucleic acid from the substrate; and (e) a process for further purifying the nucleic acid, by precipitating the desorbed nucleic acid from the solution of the process (d) and then isolating the precipitated nucleic acid. COPYRIGHT: (C)2005,JPO&NCIPI
摘要翻译：要解决的问题：提供一种纯化核酸的方法，通过用在水性吸附溶液中使用的物质代替加速将核酸粘合到诸如无机载体材料的基材上，与另一种物质。解决方案：用于纯化核酸的方法包括以下方法（a）至（d）和任选方法（e）：（a）制备从含有（i）缓冲水溶液，（ii）高浓度的盐，（iii）具有由式I表示的官能团的水可混溶的非酸性有机化合物（W是C或S; Z是O或 N），和（iv）吸附在基质上的核酸; （b）任选地通过洗涤溶液洗涤含有吸附核酸的底物的方法; （c）当与方法（a）中的组合物相比时，通过使含有吸附的核酸的底物与含有浓度较低的盐的溶液接触，使核酸脱离底物的方法; （d）通过从底物中分离含有解吸的核酸的溶液来纯化核酸的方法; 和（e）通过从方法（d）的溶液沉淀解吸的核酸，然后分离沉淀的核酸，进一步纯化核酸的方法。版权所有（C）2005，JPO＆NCIPI

8. 发明专利

JP2009261405A Improved method for treatment of bisulfite 审中-公开
标题翻译：改进双酚A的方法
公开(公告)号：JP2009261405A
公开(公告)日：2009-11-12
申请号：JP2009150412
申请日：2009-06-25
申请人： F Hoffmann La Roche Ag , エフ.ホフマン−ラロシュアーゲーF. Hoffmann−La Roche Aktiengesellschaft
发明人： BERGMANN FRANK , MARKET-HAHN CHRISTINE , KLEIBER JOERG , BLOCK DIRK
IPC分类号： C12Q1/68 , C01D5/00 , C01D5/14 , C07H21/04 , C12N15/09
CPC分类号： C07H21/04 , C01D5/145 , C12Q1/6827 , C12Q2527/101 , C12Q2523/125
摘要： PROBLEM TO BE SOLVED: To provide a method for performing a bisulfite reaction to determine methylation positions in a nucleic acid, i.e., methylated and non-methylated cytosines. SOLUTION: Provided is the method for performing a bisulfite reaction, whereby the nucleic acid is incubated in a solution comprising the nucleic acid for a time period of 1.5 to 3.5 hours at a temperature between 70 and 90°C, whereby the concentration of bisulfite in the solution is between 3 M and 6.25 M and whereby the pH value of the solution is between 5.0 and 6.0 whereby the nucleic acid, i.e., the cytosine bases in the nucleic acid, are deaminated. Then the solution comprising the deaminated nucleic acid is desulfonated. A kit comprising the solution having a specific pH value and the bisulfite is disclosed. COPYRIGHT: (C)2010,JPO&INPIT
摘要翻译：待解决的问题：提供一种进行亚硫酸氢盐反应以确定核酸中甲基化位置的方法，即甲基化和非甲基化胞嘧啶。解决方案：提供了进行亚硫酸氢盐反应的方法，由此将核酸在包含核酸的溶液中在70至90℃的温度下温育1.5至3.5小时的时间段，由此浓度的亚硫酸氢盐在3 M和6.25 M之间，由此溶液的pH值在5.0和6.0之间，由此核酸，即核酸中的胞嘧啶碱基被脱氨。然后将包含脱氨酸核酸的溶液脱磺化。公开了包含具有特定pH值的溶液和亚硫酸氢盐的试剂盒。版权所有（C）2010，JPO＆INPIT

9. 发明专利

JP2009149605A Six membered ring-having nucleotide analogue 审中-公开
标题翻译：六个会员环的核心模拟
公开(公告)号：JP2009149605A
公开(公告)日：2009-07-09
申请号：JP2008273072
申请日：2008-10-23
申请人： F Hoffmann La Roche Ag , エフ.ホフマン−ラロシュアーゲーF. Hoffmann−La Roche Aktiengesellschaft
发明人： BERGMANN FRANK , DONNER HORST , VON DER ELTZ HERBERT , HEINDL DIETER , HERDEWIJN PIET
IPC分类号： C07F9/6561 , G01N33/53 , A61K31/513 , A61K31/52 , A61K31/675 , C07D405/04 , C07D495/04 , C07H19/06 , C07H19/10 , C07H19/16 , C07H21/00 , C07H21/04 , C12N15/09 , C12P19/30 , C12P19/34 , C12P19/38 , C12Q1/68 , G01N21/78 , G01N33/566 , G01N33/58 , G01N37/00
CPC分类号： A61K31/513 , A61K31/52 , A61K31/675 , C07D405/04 , C07D495/04 , C07H19/06 , C07H19/10 , C07H19/16 , C07H21/00 , C07H21/04 , C12P19/34 , C12P19/38 , C12Q1/68 , G01N33/53 , G01N33/566 , G01N33/58 , G01N37/00 , Y02P20/55
摘要： PROBLEM TO BE SOLVED: To provide a new compound which is used for incorporating a label into a nucleic acid or affects the characteristics thereof. SOLUTION: Provided is the compound having formula I, wherein, L is a connection portion; B is a heterocyclic base; R 1 is a label or a solid phase; R 2 is selected from the group consisting of -H and -OR 6 ; R 3 is (1) a protecting group, (2) a connection portion covalently bound to the solid phase, (3) phosphoramidite, (4) H-phosphonate, or (5) triphosphate; R 4 is a connection portion; R 6 is selected from the group consisting of (1)-H, (2) a protecting group, (3) a connection portion covalently bound to the solid phase; (4) phosphoramidite, and (5) H-phosphonate; Y is O, S, NR 5 and CR 5a R 5b ; and R 5 is selected from an alkyl or the like. COPYRIGHT: (C)2009,JPO&INPIT
摘要翻译：要解决的问题：提供用于将标记掺入核酸中或影响其特征的新化合物。解决方案：提供具有式I的化合物，其中L是连接部分; B是杂环基; R 1 是标签或固相; R 2 选自-H和-OR 6 ; （3）亚磷酰胺，（4）H-膦酸盐或（5）三磷酸盐;（3）亚磷酰胺，（4） R 4 是连接部分; R 6选自（1）-H，（2）保护基，（3）与固相共价结合的连接部分; （4）亚磷酰胺，和（5）H-膦酸盐; Y是O，S，NR 5 和CR 5a R 5b ; 并且R 5选自烷基等。版权所有（C）2009，JPO＆INPIT

10. 发明专利

JP2007224028A Improved labeling reagent 有权
标题翻译：改进的标签试剂
公开(公告)号：JP2007224028A
公开(公告)日：2007-09-06
申请号：JP2007035616
申请日：2007-02-16
申请人： F Hoffmann La Roche Ag , エフ.ホフマン−ラロシュアーゲーF. Hoffmann−La Roche Aktiengesellschaft
发明人： BERGMANN FRANK , HEINDL DIETER , JOSEL HANS-PETER DR , KESSLER DIRK , LAMPERT GUNTER , WEILKE CHRISTIAN
IPC分类号： C07H21/04 , C12N15/09 , C12Q1/68
CPC分类号： C07H19/23 , C07H19/04 , C07H21/00 , C07H21/04 , Y02P20/55
摘要： PROBLEM TO BE SOLVED: To provide a probe improved for the amplification, detection and determination of nucleic acid. SOLUTION: This reagent of a compound having formula (I) is characterized in that A and B are each independent to each other, and independent from R and X [wherein, the A and B are selected from a group consisting of (1) hydrogen, (2) a protecting group, (3) a solid phase having a linker, (4) a phosphoramidite, (5) a H-phosphonate, (6) triphosphoric acid, (7) phosphoric acid and (8) a nucleotide residue chain, and provided that the A can be the triphosphoric acid but the B can not be the triphosphoric acid, and also one of the A and B is the phosphoramidite or H-phosphonate, etc]. COPYRIGHT: (C)2007,JPO&INPIT
摘要翻译：要解决的问题：提供改进用于扩增，检测和测定核酸的探针。解决方案：具有式（I）的化合物的该试剂的特征在于A和B各自独立，并且独立于R和X [其中，A和B选自（ 1）氢，（2）保护基，（3）具有接头的固相，（4）亚磷酰胺，（5）H-膦酸盐，（6）三磷酸，（7）磷酸和（8）核苷酸残基链，并且A可以是三磷酸，但B不能是三磷酸，并且A和B中的一个是亚磷酰胺或H-膦酸盐等]。版权所有（C）2007，JPO＆INPIT

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