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    • 4. 发明公开
    • NOVEL METHODS FOR RAPID DRUG SUSCEPTIBILITY TESTING AND DRUG SCREENS FOR GROWING AND NON-GROWING CELLS OF PROKARYOTIC AND EUKARYOTIC ORIGIN
    • 用于快速药物敏感性试验和药物屏障的新方法用于生长和非生长的原核和真核生成细胞
    • EP3321371A1
    • 2018-05-16
    • EP17159022.7
    • 2017-03-02
    • Zhang, YingFeng, JieYee, RebeccaZhang, ShuoZhang, WenhongWang, Xiaodong
    • ZHANG, YingFENG, JieYEE, RebeccaZHANG, WenhongZHANG, Shuo
    • C12Q1/18G01N33/50
    • G01N33/5014C12Q1/18Y02A50/57
    • The presently disclosed subject matter provides methods, compositions, and kits for extremely rapid assessment of drug susceptibility of growing as well as non-growing cells of prokaryotic and also eukaryotic origins, without the need for organism growth, which is in contrast to the current growth-dependent methods.This growth-independent new methodology achieves extremely rapid drug susceptibility testing results previously not possible. Methods for rapidly identifying compounds with inhibitory activity against both growing and non-growing cells in real-time continuous manner with previously unachievable efficiency in drug screens using the novel methodology are also provided. Cell cultures are incubated with a drug or test agent and with a staining mixture containing a first fluorescent dye indicative the emission of which is indicative of live cells (e.g. SYBR Green I) and a second fluorescent dye indicative emitting a different color, the emission of which is indicative of dead cells (e.g. Propidium iodide). The ratio of the intensities of the emitted fluorescence of the dyes is indicative of the percentage live and dead cells in the culture.
    • 本发明公开的主题提供了方法,组合物和试剂盒,用于非常快速评估原核生物和真核生物起源的生长以及非生长细胞的药物敏感性,而不需要生物体生长,这与目前的生长相反 这种独立于生长的新方法实现了以前不可能的极快的药物敏感性测试结果。 还提供了用实时连续方式迅速鉴定具有抑制生长和非生长细胞的活性的化合物的方法,以及使用新方法在药物筛选中以前无法实现的效率。 将细胞培养物与药物或测试试剂以及含有第一荧光染料和第二荧光染料的染色混合物孵育,所述第一荧光染料指示其发射指示活细胞(例如SYBR Green I),第二荧光染料指示发射不同颜色, 这是死细胞的指示(例如碘化丙锭)。 染料荧光发射强度的比例表明培养物中活细胞和死细胞的百分比。
    • 7. 发明申请
    • SURFACE NANOFABRICATION METHODS USING SELF-ASSEMBLED POLYMER NANOMASKS
    • 使用自组装聚合物纳米颗粒的表面纳米化方法
    • WO2014116547A1
    • 2014-07-31
    • PCT/US2014/012228
    • 2014-01-21
    • CORNING INCORPORATEDQUESADA, Mark AlejandroWANG, JianguoZHANG, Ying
    • QUESADA, Mark AlejandroWANG, JianguoZHANG, Ying
    • G03F7/00
    • B05D5/00B82Y10/00B82Y40/00G03F7/0002Y10S977/888
    • Methods for fabricating a nanopillared substrate surface include applying a polymer solution containing an amphiphilic block copolymer and a hydrophilic homopolymer to a substrate surface. The amphiphilic block copolymer and the hydrophilic homopolymer in the polymer solution self-assemble on the substrate surface to form a self-assembled polymer layer having hydrophobic domains adjacent to the substrate surface and hydrophilic domains extending into the self-assembled polymer layer. At least a portion of the hydrophilic domains may be removed to form a plurality of pores in the exposed surface of the self-assembled polymer layer. A protective layer may be deposited on the exposed surface as a mask for etching through the plurality of pores to form through-holes. A nanopillar-forming material may be deposited onto the substrate surface via the through-holes. Then, the remaining portion of the self-assembled polymer layer may be removed to expose a nanopillared substrate surface.
    • 用于制造纳米圆柱形基底表面的方法包括将含有两亲嵌段共聚物和亲水均聚物的聚合物溶液施加到基底表面。 聚合物溶液中的两亲性嵌段共聚物和亲水均聚物自组装在基材表面上以形成具有与基材表面相邻的疏水区域和延伸到自组装聚合物层中的亲水区域的自组装聚合物层。 亲水区域的至少一部分可以被去除以在自组装聚合物层的暴露表面中形成多个孔。 可以在暴露的表面上沉积保护层作为用于通过多个孔蚀刻的掩模以形成通孔。 可以通过通孔将纳米柱形成材料沉积到衬底表面上。 然后,可以除去自组装聚合物层的剩余部分以暴露出纳米圆柱形的衬底表面。
    • 9. 发明申请
    • NETWORK-WIDE FLOW MONITORING IN SPLIT ARCHITECTURE NETWORKS
    • 分布式架构网络中的网络流量监控
    • WO2013038279A1
    • 2013-03-21
    • PCT/IB2012/054030
    • 2012-08-07
    • TELEFONAKTIEBOLAGET L M ERICSSON (PUBL)ZHANG, Ying
    • ZHANG, Ying
    • H04L12/26
    • H04L43/026H04L43/04H04L43/0876H04L43/12
    • Flow monitoring tasks are assigned to a set of switches in a split architecture network to optimize network-wide flow monitoring. The assignment maximizes the number of monitored flows and reduces overhead of the flow monitoring. A controller receives an estimated traffic volume for each path in the network. The controller calculates, for all of the switches and all of the paths, sampling fractions that maximize the number of the flows sampled by the switches. In response to a request for setting up a new flow to traverse one of the paths in the network, the controller assigns the new flow to one of the switches that are located on the one of the paths, based on the sampling fraction for the assigned switch and the one of the paths, the bandwidth constraint and the memory constraint.
    • 分流架构网络中的一组交换机分配了流量监控任务,以优化全网络流量监控。 分配最大化监视流量的数量,并减少流量监控的开销。 控制器接收网络中每个路径的估计交通量。 控制器为所有交换机和所有路径计算采样分数,以使由交换机采样的流量数量最大化。 响应于设置新流程以遍历网络中的一条路径的请求,控制器基于所分配的采样分数将新流量分配给位于一条路径上的交换机之一 切换和路径之一,带宽约束和内存约束。