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    • 10. 发明申请
    • PROTEASE FOR WOUND CONDITIONING AND SKIN CARE
    • 蛋白酶对伤口调理和皮肤护理
    • WO2010099955A1
    • 2010-09-10
    • PCT/EP2010/001328
    • 2010-03-03
    • B.R.I.A.N. BIOTECHNOLOGY RESEARCH AND INFORMATION NETWORK AGNIEHAUS, FrankECK, JürgenSCHULZE, Renate
    • NIEHAUS, FrankECK, JürgenSCHULZE, Renate
    • C12N9/64C07K16/40A61K38/43C12N15/62C12N5/10
    • C12N9/6408
    • We have identified by molecular cloning a protease which originates from the larvae of Lucilia sericata and which was termed debrilase due to its activities useful for debridement of wounds. Described is a nucleic acid molecule encoding a serine protease having the ability to cleave fibrin and casein which is (a) a nucleic acid molecule encoding the serine protease comprising or consisting of the amino acid sequence of SEQ ID NO: 4 as well as to nucleic acid molecules encoding precursors or fragments of said serine protease; (b) a nucleic acid molecule comprising or consisting of the nucleotide sequence of SEQ ID NO: 3; (c) a nucleic acid molecule encoding a serine protease the amino acid sequence of which is at least 80 % identical to the amino acid sequence of (a), preferably at least 85 % identical, more preferably at least 90 % identical, and most preferred 95% identical; (d) a nucleic acid molecule comprising or consisting of a nucleotide sequence which is at least 80 % identical to the nucleotide sequence of (b), preferably at least 85 % identical, more preferably at least 90 % identical, and most preferred 95% identical; (e) a nucleic acid molecule which is degenerate with respect to the nucleic acid molecule of (b) or (d); or (f) a nucleic acid molecule corresponding to the nucleic acid molecule of any one of (a) to (d) wherein T is replaced by U.
    • 我们已经通过分子克隆鉴定了来源于丝光绿蝇幼虫的蛋白酶,并且由于其活性可用于伤口清创,因此被称为清创酶。 描述了编码丝氨酸蛋白酶的核酸分子,所述丝氨酸蛋白酶具有切割纤维蛋白和酪蛋白的能力,所述纤维蛋白和酪蛋白是(a)编码包含SEQ ID NO:4的氨基酸序列或由SEQ ID NO:4的氨基酸序列组成的丝氨酸蛋白酶的核酸分子, 编码所述丝氨酸蛋白酶的前体或片段的酸分子; (b)包含SEQ ID NO:3的核苷酸序列或由其组成的核酸分子; (c)编码丝氨酸蛋白酶的核酸分子,其氨基酸序列与(a)的氨基酸序列至少80%相同,优选至少85%相同,更优选至少90%相同,且大多数 优选的95%相同; (d)包含与(b)的核苷酸序列至少80%相同,优选至少85%相同,更优选至少90%相同,并且最优选95%相同的核苷酸序列或由其组成的核酸分子, 相同; (e)相对于(b)或(d)的核酸分子简并的核酸分子; 或(f)对应于(a)至(d)中任一项的核酸分子的核酸分子,其中T被U取代。