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SG11201908541SA BONE MARROW MICROFLUIDIC DEVICES AND METHODS FOR PREPARING AND USING THE SAME 未知
公开(公告)号：SG11201908541SA
公开(公告)日：2019-10-30
申请号：SG11201908541S
申请日：2018-03-26
申请人： HARVARD COLLEGE , MASSACHUSETTS GEN HOSPITAL
发明人： CHOU DAVID , LEIJTEN LILIANA , RECH ARIANNA , NOVAK RICHARD , INGBER DONALD , MILTON YUKA , FRISMANTAS VIKTORAS , LEVY OREN
IPC分类号： B01D59/36 , B01J19/00 , C12M1/00 , C12N5/00 , C12Q1/02 , C12Q1/68 , C12Q1/70
摘要： INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (43) International Publication Date 27 September 2018 (27.09.2018) WIP0 I PCT ~~ll~~~~~~~~ 011101010VIIIOH olo olomiloonmll oo mom ow (10) International Publication Number WO 2018/176043 Al (51) International Patent Classification: SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Published: — with international search report (Art. 21(3)) BOlD 59/36 (2006.01) C12Q 1/02 BO1J 19/00 (2006.01) C12Q 1/68 C12M 1/00 (2006.01) C12Q 1/70 C12N 5/00 (2006.01) (2006.01) (2018.01) (2006.01) (21) International Application Number: PCT/US2018/024377 (22) International Filing Date: 26 March 2018 (26.03.2018) (25) Filing Language: English (26) Publication Language: English (30) Priority Data: 62/476,205 24 March 2017 (24.03.2017) US (71) Applicants: PRESIDENT AND FELLOWS OF HAR- VARD COLLEGE [US/US]; 17 Quincy Street, Cam- bridge, Massachusetts 02138 (US). THE GENERAL HOSPITAL CORPORATION [US/US]; 55 Fruit Street, Boston, Massachusetts 02114 (US). = (72) Inventors: CHOU, David, Benson; 150 Staniford Street, Unit 429, Boston, Massachusetts 02114 (US). LEIJTEN, Liliana, S. Teixeira Moreira; Haspengouw 55R, Maas- tricht, 6227 RM Limburg (NL). RECH, Arianna; 525 New Ashby Road, Loughborough Leicestershire LE11 4EX (GB). NOVAK, Richard; 42 Rockview Street, Apt. 4, Ja- maica Plain, Massachusetts 02130 (US). INGBER, Don- ald, E.; 162 W. Canton St., Boston, Massachusetts 02118 (US). MILTON, Yuka; 223 Charles Street, Apt. 1, Cam- bridge, Massachusetts 02141 (US). FRISMANTAS, Vik- toras; 20 Fisher Ave., Apt. 1, Roxburg Crossing, Massa- chusetts 02120 (US). LEVY, Oren; 1386 Beacon St., Apt. 15, Brookline, Massachusetts 02446 (US). (74) Agent: RESNICK, David, S. et al.; Nixon Peabody LLP, 70 W. Madison St., Suite 3500, Chicago, Illinois 60602-4224 (US). 1-1 71' O N 1-1 00 1-1 O N C (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, (54) Title: BONE MARROW MICROFLUIDIC DEVICES AND METHODS FOR PREPARING AND USING THE SAME (57) : The present disclosure relates to a microfluidic devices and methods for culturing bone marrow cells. Aspects include methods of preparing microfluidic devices and culturing bone marrow cells with the microfluidic devices. In some aspects, a method includes providing a microfluidic device having an upper chamber, a lower chamber, and a porous membrane separating the upper chamber from the lower chamber. The method further includes seeding walls of the lower chamber and a bottom surface of the membrane with endothelial cells. The method further includes providing a matrix within the upper chamber. The matrix includes fibrin gel and bone marrow cells. The method further includes filling or perfusing the upper chamber with a media.

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