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1. 发明申请

WO2008120218A2 UNDIFFERENTIATED STEM CELL CULTURE SYSTEMS 审中-公开
标题翻译：不稳定的干细胞培养系统
公开(公告)号：WO2008120218A2
公开(公告)日：2008-10-09
申请号：PCT/IL2008000460
申请日：2008-04-02
申请人： HADASIT MED RES SERVICE , REUBINOFF BENJAMIN , STEINER DEBORA
发明人： REUBINOFF BENJAMIN , STEINER DEBORA
IPC分类号： C12N5/0735
CPC分类号： C12N5/0606 , C12N2501/115 , C12N2501/119 , C12N2501/13 , C12N2501/155 , C12N2501/16 , C12N2502/1323 , C12N2533/52 , C12N2533/54
摘要： The present disclosure provides methods for maintaining and propagating undifferentiated pluripotent stem cells (SC) in suspension. The methods comprise culturing such SC in a non-adherent culture dish under conditions comprising a basic serum free medium and one or more of a basic medium, a serum replacement, an extra cellular matrix component and a factor supporting expansion of said SC. A specific and preferred culture condition comprise supplementing Neurobasal medium with KO serum replacement (KOSR). These conditions allowed for large scale and long term propagation of undifferentiated pluripotent SC. The culture system comprising suspended undifferentiated pluripotent SC were found to have many applications including in methods for directed as well as spontaneous differentiation of the SC into somatic cells. Also disclosed herein is a method of deriving SC, preferably human embryonic SC from human embryos via the formation of cell clusters.
摘要翻译：本公开提供了用于在悬浮液中维持和增殖未分化的多能干细胞（SC）的方法。所述方法包括在包含碱性无血清培养基和一种或多种碱性培养基，血清替代物，细胞外基质组分和支持所述SC扩张的因子的条件下，在非贴壁培养皿中培养这样的SC。特定和优选的培养条件包括补充有KO血清替代物（KOSR）的神经巴氏培养基。这些条件允许未分化多能SC的大规模和长期繁殖。发现包含悬浮的未分化多能SC的培养系统具有许多应用，包括用于定向和自发分化为体细胞的方法。本文还公开了通过细胞簇的形成从人胚胎衍生SC，优选人胚胎SC的方法。

2. 发明申请

WO2006070370A3 STEM CELLS CULTURE SYSTEMS 审中-公开
标题翻译：干细胞培养系统
公开(公告)号：WO2006070370A3
公开(公告)日：2007-03-08
申请号：PCT/IL2005001397
申请日：2005-12-29
申请人： HADASIT MED RES SERVICE , BEN SHUSHAN ETTI , TANNENBAUM SHELLY , ITSYKSON PAVEL , BANIN EYAL , REUBINOFF BENJAMIN
发明人： BEN SHUSHAN ETTI , TANNENBAUM SHELLY , ITSYKSON PAVEL , BANIN EYAL , REUBINOFF BENJAMIN
IPC分类号： C12N5/00 , C12N5/071 , C12N5/0735
CPC分类号： C12N5/0606 , C12N5/0621 , C12N2500/32 , C12N2500/38 , C12N2500/44 , C12N2500/90 , C12N2500/98 , C12N2500/99 , C12N2501/115 , C12N2501/155 , C12N2501/16 , C12N2502/13 , C12N2506/02 , C12N2533/52
摘要： The present invention concerns systems and methods for providing human cell cultures. Specific embodiments of the invention relate to cultures of feeder cells for use in stem cell technology, as well as cultures, culture systems and methods for maintenance and propagating of stem cells in an undifferentiated state as well as for the development of somatic cells cultures from stem cells, the somatic cell cultures being free of extraembryonic cells.
摘要翻译：本发明涉及用于提供人类细胞培养物的系统和方法。本发明的具体实施方案涉及用于干细胞技术的饲养细胞的培养物，以及用于维持和增殖未分化状态的干细胞的培养物，培养系统和方法，以及用于从茎发育的体细胞培养物细胞，体细胞培养物不含胚胎细胞。

3. 发明申请

WO2009057111A3 HUMAN STEM CELL-DERIVED NEURAL PRECURSORS FOR TREATMENT OF AUTOIMMUNE DISEASES OF THE CENTRAL NERVOUS SYSTEM 审中-公开
标题翻译：人类干细胞衍生的神经前体治疗中枢神经系统的自身免疫性疾病
公开(公告)号：WO2009057111A3
公开(公告)日：2010-05-27
申请号：PCT/IL2008001426
申请日：2008-10-29
申请人： HADASIT MED RES SERVICE , AHARONOWIZ MICHAL , EINSTEIN OFIRA , REUBINOFF BENJAMIN , BEN-HUR TAMIR
发明人： AHARONOWIZ MICHAL , EINSTEIN OFIRA , REUBINOFF BENJAMIN , BEN-HUR TAMIR
IPC分类号： A61K35/12 , A61P25/28
CPC分类号： C12N5/0623 , A61K35/30 , A61K2035/122 , C12N2501/105 , C12N2501/11 , C12N2501/115 , C12N2501/13 , C12N2501/135 , C12N2501/385 , C12N2501/395 , C12N2501/41 , C12N2501/70 , C12N2506/02
摘要： The present invention concerns the use of a population of cells comprising: (a) neural precursor cells committed to an oligodendroglial fate; (b) uncommitted neural precursor cells (c) differentiated oligodendrocytes; or (d) a combination of any one of (a) to (c) for the treatment of CNS autoimmune diseases, or for the preparation of a pharmaceutical composition for treating CNS autoimmune diseases, the population of cells being derived from human pluripotent stem cells. The invention also provides methods for obtaining such populations of cells, namely, neural precursor cells committed to an oligodendroglial fate as well as differentiated oligodendrocytes which then can be used in the treatment of CNS autoimmune diseases. A preferred autoimmune disease in the context of the present invention is multiple sclerosis where the population of cells is administered to the CNS for local treatment of the disease.
摘要翻译：本发明涉及细胞群的用途，其包括：（a）承载于少突神经胶质细胞命运的神经前体细胞; （b）未提及的神经前体细胞（c）分化的少突胶质细胞; 或（d）（a）至（c）中任一项的组合用于治疗CNS自身免疫性疾病，或用于制备用于治疗CNS自身免疫疾病的药物组合物，所述细胞群源自人多能干细胞。本发明还提供了获得这种细胞群的方法，即致死少突胶质细胞命运的神经前体细胞以及分化的少突胶质细胞，其然后可用于治疗CNS自身免疫性疾病。在本发明的上下文中优选的自身免疫性疾病是多发性硬化，其中细胞群被施用于CNS以局部治疗该疾病。

4. 发明公开

EP2142641A4 UNDIFFERENTIATED STEM CELL CULTURE SYSTEMS 审中-公开
标题翻译：未分化的干细胞培养系统
公开(公告)号：EP2142641A4
公开(公告)日：2010-08-18
申请号：EP08738164
申请日：2008-04-02
申请人： HADASIT MED RES SERVICE
发明人： REUBINOFF BENJAMIN , STEINER DEBORA
IPC分类号： C12N5/0735
CPC分类号： C12N5/0606 , C12N2501/115 , C12N2501/119 , C12N2501/13 , C12N2501/155 , C12N2501/16 , C12N2502/1323 , C12N2533/52 , C12N2533/54

5. 发明专利

HK1179647A1 STEM CELL-DERIVED RETINAL PIGMENT EPITHELIAL CELLS 未知
公开(公告)号：HK1179647A1
公开(公告)日：2013-10-04
申请号：HK13106571
申请日：2013-06-04
申请人： HADASIT MED RES SERVICE
发明人： IDELSON MASHA , ALPER-PINUS RUSLANA , OBOLENSKY ALEX , BANIN EYAL , REUBINOFF BENJAMIN
IPC分类号： A61K35/44 , A61K35/48 , C12N5/071
摘要： The present invention relates to a method of differentiating human pluripotent stem cells into retinal pigment epithelium (RPE) cells, the method comprising: a. culturing the human pluripotent stem cells in a medium supplemented with nicotinamide (NA) to generate differentiating cells; and thereafter b. culturing said differentiating cells in a culture system comprising one or more members of the TGF β superfamily, whereby said human pluripotent stem cells are induced to differentiate into RPE cells.

6. 发明专利

HK1140791A1 STEM CELL-DERIVED RETINAL PIGMENT EPITHELIAL CELLS 未知
公开(公告)号：HK1140791A1
公开(公告)日：2010-10-22
申请号：HK10107017
申请日：2010-07-20
申请人： HADASIT MED RES SERVICE
发明人： IDELSON MASHA , ALPER-PINUS RUSLANA , OBOLENSKY ALEX , BANIN EYAL , REUBINOFF BENJAMIN
IPC分类号： A61K35/44 , A61K35/48 , C12N5/071
摘要： The present invention relates to a method of differentiating human pluripotent stem cells into retinal pigment epithelium (RPE) cells, the method comprising: a. culturing the human pluripotent stem cells in a medium supplemented with nicotinamide (NA) to generate differentiating cells; and thereafter b. culturing said differentiating cells in a culture system comprising one or more members of the TGF β superfamily, whereby said human pluripotent stem cells are induced to differentiate into RPE cells.

7. 发明专利

AU2008242106A1 Stem cell-derived retinal pigment epithelial cells 未知
公开(公告)号：AU2008242106A1
公开(公告)日：2008-10-30
申请号：AU2008242106
申请日：2008-04-27
申请人： HADASIT MED RES SERVICE
发明人： OBOLENSKY ALEX , ALPER-PINUS RUSLANA , REUBINOFF BENJAMIN , BANIN EYAL , IDELSON MASHA
IPC分类号： A61K35/44 , A61K35/48 , C12N5/071
摘要： The present invention relates to a method of differentiating human pluripotent stem cells into retinal pigment epithelium (RPE) cells, the method comprising: a. culturing the human pluripotent stem cells in a medium supplemented with nicotinamide (NA) to generate differentiating cells; and thereafter b. culturing said differentiating cells in a culture system comprising one or more members of the TGF β superfamily, whereby said human pluripotent stem cells are induced to differentiate into RPE cells.

8. 发明专利

CA2684460A1 STEM CELL-DERIVED RETINAL PIGMENT EPITHELIAL CELLS 未知
公开(公告)号：CA2684460A1
公开(公告)日：2008-10-30
申请号：CA2684460
申请日：2008-04-27
申请人： HADASIT MED RES SERVICE
发明人： OBOLENSKY ALEX , REUBINOFF BENJAMIN , BANIN EYAL , IDELSON MASHA , ALPER-PINUS RUSLANA
IPC分类号： A61K35/44 , A61K35/48 , C12N5/071
摘要： The present invention concerns RPE cells obtainable by directed different iation from stem cell, particularly, human stem cells. It has been specifica lly found that culturing stem cells in the presence of one or more member of the TGF superfamily, such as Activin A) induced directed differentiation in to mature and functional RPE cells. This was evidenced by the expression of markers specific to mature RPE cells, including MiTF-A, RPE65 or Bestrophin) . In accordance with one particular embodiment, the cells are a priori cultu red with nicotinamide (NA) which was found to augment the cells' response to the inductive effect of the one or more member of the TGF superfamily. The invention also provides methods of performing the directed differentiation, as well as methods for use of the resulting RPE cells.

9. 发明专利

ES2527444T3 Células del epitelio pigmentario de la retina derivadas de células madre 未知
公开(公告)号：ES2527444T3
公开(公告)日：2015-01-23
申请号：ES08738258
申请日：2008-04-27
申请人： HADASIT MED RES SERVICE
发明人： IDELSON MASHA , ALPER-PINUS RUSLANA , OBOLENSKY ALEX , BANIN EYAL , REUBINOFF BENJAMIN
IPC分类号： C12N5/079 , A61K35/44 , A61K35/48 , C12N5/071
摘要： Un método de inducción de la diferenciación dirigida de células madre pluripotentes humanas en células del epitelio pigmentario de la retina, método que comprende: a. cultivar células madre pluripotentes humanas en un sistema de cultivo que comprende un medio básico suplementado con nicotinamida (NA) para generar células en diferenciación; y b. cultivar dichas células en diferenciación en un sistema de cultivo que comprende un medio básico suplementado con uno o más miembros de la superfamilia de factores de crecimiento de transformación TGFβ, mediante lo que dichas células madre pluripotentes humanas son inducidas a la diferenciación en células RPE.

10. 发明专利

AU2008242106B2 Stem cell-derived retinal pigment epithelial cells 未知
公开(公告)号：AU2008242106B2
公开(公告)日：2013-05-16
申请号：AU2008242106
申请日：2008-04-27
申请人： HADASIT MED RES SERVICE
发明人： ALPER-PINUS RUSLANA , BANIN EYAL , IDELSON MASHA , REUBINOFF BENJAMIN , OBOLENSKY ALEX
IPC分类号： C12N5/071 , A61K35/44 , A61K35/48
摘要： The present invention concerns RPE cells obtainable by directed differentiation from stem cell, particularly, human stem cells. It has been specifically found that culturing stem cells in the presence of one or more member of the TGF superfamily, such as Activin A) induced directed differentiation into mature and functional RPE cells. This was evidenced by the expression of markers specific to mature RPE cells, including MiTF-A, RPE65 or Bestrophin). In accordance with one particular embodiment, the cells are a priori cultured with nicotinamide (NA) which was found to augment the cells' response to the inductive effect of the one or more member of the TGF superfamily. The invention also provides methods of performing the directed differentiation, as well as methods for use of the resulting RPE cells.

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