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    • 1. 发明申请
    • Fast spectral confocal imager
    • 快速光谱共焦成像仪
    • US20060238756A1
    • 2006-10-26
    • US11351558
    • 2006-02-10
    • Gregory BearmanDaniel WilsonScott Fraser
    • Gregory BearmanDaniel WilsonScott Fraser
    • G01J3/30G01J3/28
    • G01J3/2823G01J3/18G01J3/4406G01N21/6458G01N2021/6423G02B21/0064G02B21/0076
    • Fast confocal spectral imagers are provided. A fast confocal spectral imager according to the invention includes a spectral imager coupled to a fast confocal microscope. A laser is provided for generating laser light, which passes through scanning optics which are configured to scan a line- or slit-shaped region of a specimen at a given time. The light then passes through an objective lens and excites fluorescent dyes applied to the specimen, causing the dyes to fluoresce at respective emission spectra. The fluorescence radiated by the excited dyes then passes back through the scanning optics and is directed to a fixed slit that functions as an entrance slit for a spectral imager. The spectral imager receives the fluorescence and separates it into wavelength bands. The wavelength and position across the slit-shaped region of the specimen for each wavelength band are then recorded.
    • 提供快速共聚焦光谱成像仪。 根据本发明的快速共焦光谱成像仪包括耦合到快速共聚焦显微镜的光谱成像仪。 提供了用于产生激光的激光,其通过扫描光学器件,扫描光学器件被配置为在给定时间扫描样品的线或狭缝形区域。 然后光通过物镜并激发施加到样品上的荧光染料,导致染料在各自的发射光谱下发荧光。 由激发的染料辐射的荧光然后通过扫描光学器件返回,并被引导到用作光谱成像器的入口狭缝的固定狭缝。 光谱成像仪接收荧光并将其分离成波段。 然后记录每个波长带的样品的狭缝状区域的波长和位置。