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1. 发明申请

US20110151550A1 System For Multi Color Real Time PCR 审中-公开
标题翻译：多色实时PCR系统
公开(公告)号：US20110151550A1
公开(公告)日：2011-06-23
申请号：US13023407
申请日：2011-02-08
申请人： Gregor Sagner , Ingrid Bechler , Jochen Bolte , Dieter Heindl , Hans-Peter Josel , Martin Gutekunst , Rudolf Sebl , Christoph Mueller
发明人： Gregor Sagner , Ingrid Bechler , Jochen Bolte , Dieter Heindl , Hans-Peter Josel , Martin Gutekunst , Rudolf Sebl , Christoph Mueller
IPC分类号： C12M1/34
CPC分类号： C12Q1/6851 , G01N2021/6421 , C12Q2565/101 , C12Q2561/113 , C12Q2537/143
摘要： The invention is directed to a system for performing multi-color real time PCR, comprising a flexible real time PCR instrument and a specific composition or reaction mixture for performing multiplex PCR: In particular, the present invention is directed to a composition or reaction mixture which comprises at least 3, preferably 4-5 and most preferably exactly 4 pairs of FRET hybridization probes. Each pair of said hybridization probes consists of a FRET donor probe carrying a FRET donor moiety and a FRET acceptor probe carrying a FRET acceptor moiety having an emission maximum between 550 and 710 nm.
摘要翻译：本发明涉及用于进行多色实时PCR的系统，其包括灵活的实时PCR仪器和用于进行多重PCR的特定组合物或反应混合物：特别地，本发明涉及一种组合物或反应混合物，其中包含至少3个，优选4-5个，最优选正确的4对FRET杂交探针。每对所述杂交探针由携带FRET供体部分的FRET供体探针和携带具有550和710nm之间的发射最大值的FRET受体部分的FRET受体探针组成。

2. 发明授权

US08137616B2 System for multi color real time PCR 有权
标题翻译：多色实时PCR系统
公开(公告)号：US08137616B2
公开(公告)日：2012-03-20
申请号：US10549648
申请日：2004-04-01
申请人： Gregor Sagner , Ingrid Bechler , Jochen Bolte , Dieter Heindl , Hans-Peter Josel , Martin Gutekunst , Rudolf Sebl , Christoph Mueller
发明人： Gregor Sagner , Ingrid Bechler , Jochen Bolte , Dieter Heindl , Hans-Peter Josel , Martin Gutekunst , Rudolf Sebl , Christoph Mueller
IPC分类号： C12Q1/68 , G01N15/06 , G01N33/00 , G01N33/48 , G01N33/566 , C12P19/34
CPC分类号： C12Q1/6851 , G01N2021/6421 , C12Q2565/101 , C12Q2561/113 , C12Q2537/143
摘要： The invention is directed to a system for performing multi-color real time PCT, comprising a flexible real time PCT instrument and a specific composition or reaction mixture for performing multiplex PCR: In particular, the present invention is directed to a composition or reaction mixture which comprises at least 3, preferably 4-5 and most preferably exactly 4 pairs of FRET hybridization probes. Each pair of said hybridization probes consists of a FRET donor probe carrying a FRET donor moiety and a FRET acceptor probe carrying a FRET acceptor moiety having an emission maximum between 550 and 710 nm.
摘要翻译：本发明涉及用于执行多色实时PCT的系统，其包括柔性实时PCT仪器和用于进行多重PCR的特定组合物或反应混合物：特别地，本发明涉及一种组合物或反应混合物，其中包含至少3个，优选4-5个，最优选正确的4对FRET杂交探针。每对所述杂交探针由携带FRET供体部分的FRET供体探针和携带具有550和710nm之间的发射最大值的FRET受体部分的FRET受体探针组成。

3. 发明申请

US20060269922A1 System for multi color real time pcr 有权
标题翻译：多色实时系统
公开(公告)号：US20060269922A1
公开(公告)日：2006-11-30
申请号：US10549648
申请日：2004-04-01
申请人： Gregor Sagner , Ingrid Bechler , Jochen Bolte , Dieter Heindl , Hans-Peter Josel , Martin Gutekunst , Rudolf Sebl , Christoph Mueller
发明人： Gregor Sagner , Ingrid Bechler , Jochen Bolte , Dieter Heindl , Hans-Peter Josel , Martin Gutekunst , Rudolf Sebl , Christoph Mueller
IPC分类号： C12Q1/68 , C12P19/34 , C12M1/34 , C07H21/04
CPC分类号： C12Q1/6851 , G01N2021/6421 , C12Q2565/101 , C12Q2561/113 , C12Q2537/143
摘要： The invention is directed to a system for performing multi-color real time PCT, comprising a flexible real time PCT instrument and a specific composition or reaction mixture for performing multiplex PCR: In particular, the present invention is directed to a composition or reaction mixtuer which comprises at least 3, preferably 4-5 and most preferably exactly 4 pairs of FRET hybridization probes. Each pair of said hybridization probes consists of a FRET donor probe carrying a FRET donor moiety and a FRET acceptor probe carrying a FRET acceptor moiety having an emission maximum between 550 and 710 nm.
摘要翻译：本发明涉及用于执行多色实时PCT的系统，其包括灵活的实时PCT仪器和用于进行多重PCR的特定组合物或反应混合物：特别地，本发明涉及一种组合物或反应混合器，其组合物或反应混合器包含至少3个，优选4-5个，最优选正确的4对FRET杂交探针。每对所述杂交探针由携带FRET供体部分的FRET供体探针和携带具有550和710nm之间的发射最大值的FRET受体部分的FRET受体探针组成。

4. 发明申请

US20050042618A1 Fluorescent resonance energy transfer probes 审中-公开
标题翻译：荧光共振能量转移探针
公开(公告)号：US20050042618A1
公开(公告)日：2005-02-24
申请号：US10635171
申请日：2003-08-06
申请人： Dieter Heindl , Hans-Peter Josel , Gregor Sagner , Ingrid Bechler
发明人： Dieter Heindl , Hans-Peter Josel , Gregor Sagner , Ingrid Bechler
IPC分类号： G01N33/53 , C12N15/09 , C12Q1/68 , G01N33/542 , G01N33/566 , G01N33/58 , C07H21/04
CPC分类号： C12Q1/6818
摘要： Compositions, reaction mixtures, and kits, as well as methods for their use, comprising a pair of FRET hybridization probes hybridizing adjacently to a target nucleic acid sequence, each hybridization probe comprising (i) a nucleotide sequence entity which is substantially complementary to the sequence of the target nucleic acid, (ii) a fluorescent entity, being either a FRET donor entity or a FRET acceptor entity, and (iii) a spacer entity connecting the nucleotide sequence entity and the fluorescent entity. The intensity of fluorescence emission from the FRET donor entity and from the FRET acceptor entity is not substantially affected by quenching activity of nucleotide residues present in the sequence of the target nucleic acid or in the nucleotide sequence entities of the hybridization probes.
摘要翻译：组合物，反应混合物和试剂盒及其使用方法包括与靶核酸序列相邻杂交的一对FRET杂交探针，每个杂交探针包含（i）与该序列基本互补的核苷酸序列实体的靶核酸，（ii）作为FRET供体实体或FRET受体实体的荧光实体，和（iii）连接核苷酸序列实体和荧光实体的间隔实体。来自FRET供体实体和FRET受体实体的荧光发射的强度基本上不受存在于靶核酸序列或杂交探针的核苷酸序列实体中的核苷酸残基的猝灭活性的影响。

5. 发明申请

US20050176014A1 Fluorescent hybridization probes with reduced background 审中-公开
标题翻译：具有降低背景的荧光杂交探针
公开(公告)号：US20050176014A1
公开(公告)日：2005-08-11
申请号：US10621428
申请日：2003-07-16
申请人： Dieter Heindl , Hans-Peter Josel , Gregor Sagner , Jutta Mayr
发明人： Dieter Heindl , Hans-Peter Josel , Gregor Sagner , Jutta Mayr
IPC分类号： G01N33/53 , C12N15/09 , C12Q1/68 , G01N33/566 , G01N33/58 , G01N37/00 , C07H21/04
CPC分类号： C12Q1/6818
摘要： The invention is directed to a pair of FRET hybridization probes consisting of a first oligonucleotide carrying a FRET donor entity and a second oligonucleotide carrying a FRET acceptor entity, wherein said first oligonucleotide carrying the donor fluorescent entity is carrying at least one second entity, said second entity being a compound which is capable of quenching fluorescence emission of said donor fluorescent entity. In addition, the invention is directed to methods of using the same.
摘要翻译：本发明涉及由携带FRET供体实体的第一寡核苷酸和携带FRET受体实体的第二寡核苷酸组成的一对FRET杂交探针，其中所述携带供体荧光实体的第一寡核苷酸携带至少一个第二实体，所述第二个实体实体是能够淬灭所述供体荧光实体的荧光发射的化合物。此外，本发明涉及使用该方法的方法。

6. 发明授权

US06184379B2 Rhodamine derivatives and the use thereof 有权
标题翻译：罗丹明衍生物及其用途
公开(公告)号：US06184379B2
公开(公告)日：2001-02-06
申请号：US09324265
申请日：1999-06-02
申请人： Hans-Peter Josel , Rupert Herrmann , Dieter Heindl , Klaus Muhlegger , Gregor Sagner , Karl Heinz Drexhage , Jorg Frantzeskos , Jutta Arden-Jacob
发明人： Hans-Peter Josel , Rupert Herrmann , Dieter Heindl , Klaus Muhlegger , Gregor Sagner , Karl Heinz Drexhage , Jorg Frantzeskos , Jutta Arden-Jacob
IPC分类号： C07D49147
CPC分类号： C09B11/24 , Y10S436/80
摘要： The invention concerns rhodamine derivatives of the general formulae in which Ca-Cd each denote a C atom, and Ca and Cb as well as Cc and Cd are either linked together by a single bond or by a double bond; X1 to X16 denote independently of one another halogen, sulfonic acid, hydrogen or an alkyl residue with 1-20 C atoms in which the alkyl residue can be substituted with one or several halogen or sulfonic acid residues; R1 and R2 are either identical or different and denote either hydrogen, alkyl with 1-20 C atoms, polyoxyhydrocarbyl units, phenyl or phenylalkyl with 1-3 carbon atoms in the alkyl chain in which the alkyl and/or phenyl residues can be substituted by one or several hydroxy, halogen, sulfonic acid, amino, carboxy or alkoxycarbonyl groups where alkoxy can have 1-4 carbon atoms, R1 contains at least one activatable group, R2 and X4 can be optionally linked together via a bridge composed of 0-2 C atoms. In contrast to the prior art, these compounds are characterized in that A1, A2 and A3 can independently of one another denote hydrogen, cyano, halogen and sulfonic acid; B1 denotes either halogen, cyano or hydrogen; B2 denotes hydrogen, amide, halogen and an alkyl residue with 1-20 C atoms. In addition the invention concerns activated rhodamine derivatives, correspondingly conjugated biomolecules and their use in diagnostic systems.
摘要翻译：本发明涉及一般式的罗丹明衍生物，其中Ca-Cd各自表示C原子，Ca和Cb以及C c和Cd通过单键或双键连接在一起; X1至X16彼此独立地表示卤素，磺酸，氢或具有1-20个C原子的烷基残基，其中烷基残基可被一个或几个卤素或磺酸残基取代; R 1和R 2可以相同或不同，表示氢，具有1-20个C原子的烷基，聚氧烃基单元，烷基链中具有1-3个碳原子的苯基或苯基烷基，其中烷基和/或苯基残基可被一个或几个羟基，卤素，磺酸，氨基，羧基或烷氧基羰基，其中烷氧基可以具有1-4个碳原子，R 1包含至少一个可活化基团，R 2和X 4可以任选地通过由0-2 C原子。与现有技术相反，这些化合物的特征在于，A1，A2和A3可以彼此独立地表示氢，氰基，卤素和磺酸; B1表示卤素，氰基或氢; B2表示氢，酰胺，卤素和具有1-20个C原子的烷基。此外，本发明涉及活化的罗丹明衍生物，相应的共轭生物分子及其在诊断系统中的用途。

7. 发明申请

US20070184453A1 Fret process 审中-公开
标题翻译：反应过程
公开(公告)号：US20070184453A1
公开(公告)日：2007-08-09
申请号：US10678440
申请日：2003-10-01
申请人： Gregor Sagner , Dieter Heindl , Ingrid Bechler , Christina Krause
发明人： Gregor Sagner , Dieter Heindl , Ingrid Bechler , Christina Krause
IPC分类号： C12Q1/68 , C12P19/34 , C07H21/04
CPC分类号： C12Q1/6818
摘要： The present invention is directed to hybridization probes hybridizing adjacently to another at a target nucleic acid sequence, wherein one member of said hybridization probes comprises (i) a nucleotide sequence entity which is substantially complementary to the sequence of the target nucleic acid, (ii) a fluorescent entity being either a FRET donor entity or a FRET acceptor entity, and (iii) a spacer entity connecting the nucleotide sequence entity and the fluorescent entity.
摘要翻译：本发明涉及在目标核酸序列上与另一种相邻地杂交的杂交探针，其中所述杂交探针的一个成员包含（i）与靶核酸序列基本上互补的核苷酸序列实体，（ii）荧光实体是FRET供体实体或FRET受体实体，和（iii）连接核苷酸序列实体和荧光实体的间隔实体。

8. 发明申请

US20120070840A1 RELATIVE QUANTIFICATION ANALYSIS OF MULTI-PARAMETRIC PCR EXPERIMENTS 审中-公开
标题翻译：多参数PCR实验的相对量化分析
公开(公告)号：US20120070840A1
公开(公告)日：2012-03-22
申请号：US13224423
申请日：2011-09-02
申请人： Ingrid Bechler , Gregor Sagner
发明人： Ingrid Bechler , Gregor Sagner
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6851 , G16B40/00 , C12Q2545/101 , C12Q2545/113
摘要： An analysis method for quantitative PCR experiments including multiple genes of interest, multiple samples and multiple conditions is provided. For calculating the relative expression status of multiple target genes from multiple samples under multiple different conditions, a calculation method based on up to three different parameters is performed. In addition to normalization against one or multiple reference gene(s) and normalization against a reference sample (calibrator), a third normalization step against a base value is performed in a target-, sample- and condition-specific manner.
摘要翻译：提供了包括感兴趣的多个基因，多个样品和多个条件的定量PCR实验的分析方法。为了计算多个不同条件下多个样本的多个目标基因的相对表达状态，采用基于三个不同参数的计算方法。除了针对一个或多个参考基因的标准化和针对参考样品（校准物）的归一化之外，针对基础值的第三归一化步骤以目标，样品和条件特异性方式进行。

9. 发明授权

US06691041B2 Method for the efficiency-corrected real-time quantification of nucleic acids 有权
标题翻译：核酸的效率校正实时定量的方法
公开(公告)号：US06691041B2
公开(公告)日：2004-02-10
申请号：US09823711
申请日：2001-03-30
申请人： Gregor Sagner , Karim Tabiti , Martin Gutekunst , Richie Soong
发明人： Gregor Sagner , Karim Tabiti , Martin Gutekunst , Richie Soong
IPC分类号： G01N3348
CPC分类号： G06F19/00 , C12Q1/6851 , C12Q2561/113 , C12Q2545/114 , C12Q2545/113
摘要： The present invention concerns a method for the quantification of a target nucleic acid in a sample comprising the following steps: (i) determination of the amplification efficiency of the target nucleic acid under defined amplification conditions, (ii) amplification of the target nucleic acid contained in the sample under the same defined reaction conditions, (iii) measuring the amplification in real-time, (iv) quantification of the original amount of target nucleic acid in the sample by correction of the original amount derived from step (iii) with the aid of the determined amplification efficiency. The efficiency correction of PCR reactions according to the invention for the quantification of nucleic acids can be used for absolute quantification with the aid of an external or internal standard as well as for relative quantification compared to the expression of housekeeping genes.
摘要翻译：本发明涉及用于定量样品中靶核酸的方法，包括以下步骤：（i）在确定的扩增条件下测定靶核酸的扩增效率，（ii）扩增含有的靶核酸在相同定义的反应条件下的样品中，（iii）实时测量扩增，（iv）通过用步骤（iii）得到的原始量的校正来定量样品中目标核酸的原始量，有助于确定的扩增效率。根据本发明的用于定量核酸的PCR反应的效率校正可以用于借助于外部或内部标准的绝对定量，以及与管家基因的表达相比进行相对定量。

10. 发明申请

US20100021923A1 METHOD FOR THE EFFICIENCY-CORRECTED REAL-TIME QUANTIFICATION OF NUCLEIC ACIDS 有权
标题翻译：用于有效校正核酸实时定量的方法
公开(公告)号：US20100021923A1
公开(公告)日：2010-01-28
申请号：US12549150
申请日：2009-08-27
申请人： Gregor Sagner , Karim Tabiti , Martin Gutekunst , Richie Soong
发明人： Gregor Sagner , Karim Tabiti , Martin Gutekunst , Richie Soong
IPC分类号： C12Q1/68
CPC分类号： G06F19/00 , C12Q1/6851 , C12Q2561/113 , C12Q2545/114 , C12Q2545/113
摘要： The present invention concerns a method for the quantification of a target nucleic acid in a sample comprising the following steps: (i) determination of the amplification efficiency of the target nucleic acid under defined amplification conditions, (ii) amplification of the target nucleic acid contained in the sample under the same defined reaction conditions, (iii) measuring the amplification in real-time, (iv) quantification of the original amount of target nucleic acid in the sample by correction of the original amount derived from step (iii) with the aid of the determined amplification efficiency. The efficiency correction of PCR reactions according to the invention for the quantification of nucleic acids can be used for absolute quantification with the aid of an external or internal standard as well as for relative quantification compared to the expression of housekeeping genes.
摘要翻译：本发明涉及用于定量样品中靶核酸的方法，包括以下步骤：（i）在确定的扩增条件下测定靶核酸的扩增效率，（ii）扩增含有的靶核酸在相同定义的反应条件下的样品中，（iii）实时测量扩增，（iv）通过用步骤（iii）得到的原始量的校正来定量样品中目标核酸的原始量，有助于确定的扩增效率。根据本发明的用于定量核酸的PCR反应的效率校正可以用于借助于外部或内部标准的绝对定量，以及与管家基因的表达相比进行相对定量。

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