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1. 发明授权

US06498340B2 Method for calibrating mass spectrometers 失效
标题翻译：校准质谱仪的方法
公开(公告)号：US06498340B2
公开(公告)日：2002-12-24
申请号：US09760248
申请日：2001-01-12
申请人： Gordon A. Anderson , Michael D. Brands , James E. Bruce , Ljiljana Pasa-Tolic , Richard D. Smith
发明人： Gordon A. Anderson , Michael D. Brands , James E. Bruce , Ljiljana Pasa-Tolic , Richard D. Smith
IPC分类号： H01J4900
CPC分类号： H01J49/0009
摘要： A method whereby a mass spectra generated by a mass spectrometer is calibrated by shifting the parameters used by the spectrometer to assign masses to the spectra in a manner which reconciles the signal of ions within the spectra having equal mass but differing charge states, or by reconciling ions having known differences in mass to relative values consistent with those known differences. In this manner, the mass spectrometer is calibrated without the need for standards while allowing the generation of a highly accurate mass spectra by the instrument.
摘要翻译：通过移动光谱仪使用的参数来校准光谱仪产生的质谱的方法来校准光谱仪产生的质谱，方法是以质量相同但不同电荷状态的光谱中的离子信号进行协调，或通过调和具有与已知差异一致的质量与相对值之间的已知差异的离子。以这种方式，质谱仪被校准，而不需要标准，同时允许通过仪器产生高精度的质谱。

2. 发明授权

US06629040B1 Isotope distribution encoded tags for protein identification 失效
标题翻译：用于蛋白质鉴定的同位素分布编码标签
公开(公告)号：US06629040B1
公开(公告)日：2003-09-30
申请号：US09528603
申请日：2000-03-20
申请人： David R. Goodlett , James E. Bruce , Beate Rist , Richard D. Smith , Ruedi Aebersold
发明人： David R. Goodlett , James E. Bruce , Beate Rist , Richard D. Smith , Ruedi Aebersold
IPC分类号： G01N3100
CPC分类号： G01N33/6848 , C12Q1/37 , G01N33/534 , G01N33/6815 , G01N33/6818 , G01N33/6842 , G01N2458/15
摘要： The present invention provides methods and reagents for identifying specific masses of compounds in a complex mixture. In particular, the present invention describes an isotope distribution encoded tag (IDEnT) concept wherein the ability to distinguish an analyte encoded with a non-native isotope from those not encoded with the isotope or from those encoded with a different isotope is used for protein identification, enzyme active site identification, peptide sequencing, and the like. Reagents used in the IDEnT strategy consist of a chemical element with a unique isotope distribution not normally found in the analyte class being studied, chemically bonded to, or an integral part of a chemical reagent with a high selectivity of affinity for specific functional group in the analyte.
摘要翻译：本发明提供用于鉴定复合混合物中化合物的特定质量的方法和试剂。特别地，本发明描述了同位素分布编码标签（IDEnT）概念，其中将用非天然同位素编码的分析物与未用同位素编码的分析物或由不同于同位素编码的分析物区分开的能力用于蛋白质鉴定酶活性位点鉴定，肽测序等。在IDEnT策略中使用的试剂由具有独特同位素分布的化学元素组成，该化学元素通常不被发现在被研究的分析物类别中，化学键合到化学试剂或其化学试剂的组成部分，对于特定官能团具有高亲和力选择性分析物。

3. 发明授权

US07479372B2 Beta-secretase substrates and uses thereof 失效
标题翻译： β-分泌酶底物及其用途
公开(公告)号：US07479372B2
公开(公告)日：2009-01-20
申请号：US11523507
申请日：2006-09-19
申请人： Stephen F. Brady , James E. Bruce , Elizabeth Chen-Dodson , Victor Garsky , Yueming Li , Mohinder Sardana , Jules A. Shafer , Xiaoting Tang
发明人： Stephen F. Brady , James E. Bruce , Elizabeth Chen-Dodson , Victor Garsky , Yueming Li , Mohinder Sardana , Jules A. Shafer , Xiaoting Tang
IPC分类号： G01N33/53
CPC分类号： C12Q1/37 , C07K7/06 , C07K14/4711 , C12P21/06 , G01N33/6896 , G01N2500/00
摘要： The present invention provides synthetic β-secretase peptide substrates useful in various assays for measuring β-secretase activity. Antibodies that recognize the synthetic substrates and uses of the antibodies in various assays are disclosed. The herein disclosed peptide substrates are hydrolyzed at rates substantially faster than the attendant Swedish mutant APP from which the substrate sequences are derived.
摘要翻译：本发明提供用于测量β-分泌酶活性的各种测定中的合成β-分泌酶肽底物。公开了在各种测定中识别合成底物和抗体应用的抗体。本文所公开的肽底物的水解速度明显快于从其中衍生出底物序列的瑞典突变体APP。

4. 发明授权

US06759354B1 Printable synthetic fabric 失效
标题翻译：可打印合成纤维布
公开(公告)号：US06759354B1
公开(公告)日：2004-07-06
申请号：US09941124
申请日：2001-08-28
申请人： Garnett H. Smith , James E. Bruce
发明人： Garnett H. Smith , James E. Bruce
IPC分类号： B32B2704
CPC分类号： D06P5/001 , D06B21/00 , D06M2200/50 , D06P1/16 , D06P3/54 , D06P5/2077 , D06P5/22 , Y10S8/922 , Y10S8/933 , Y10T442/2484 , Y10T442/291 , Y10T442/40
摘要： A dyed fabric, and apparel constructed therefrom, that has a high degree of colorfastness and a soft hand, formed by the process comprising the steps of applying dyestuffs directly to a polyester fabric that has been pre-treated with a wicking agent, and fixing the dyestuffs to the fabric by heating the fabric at between about 350 degrees Fahrenheit for between about 90 and 120 seconds, whereby the finished fabric has a crocking index of at least 4.0, and a soft hand.
摘要翻译：通过该方法形成的染色织物和由其构成的具有高度色牢度和柔软手感的服装，该方法包括以下步骤：将染料直接施加到已经用芯吸剂预处理的聚酯织物上，并将通过在约350华氏度之间加热织物约90至120秒，将织物染色至织物，由此成品织物具有至少4.0的摩擦指数和柔软的手感。

5. 发明申请

US20090218485A1 ION-TRAPPING DEVICES PROVIDING SHAPED RADIAL ELECTRIC FIELD 有权
标题翻译：提供形状的径向电场的离子捕获装置
公开(公告)号：US20090218485A1
公开(公告)日：2009-09-03
申请号：US12334259
申请日：2008-12-12
申请人： Nathan K. Kaiser , James E. Bruce
发明人： Nathan K. Kaiser , James E. Bruce
IPC分类号： B01D59/44
CPC分类号： H01J49/38
摘要： Disclosed are ion cyclotron resonance (ICR) cells and other ion-trapping cells with plural groups of multiple trapping electrodes for shaping (e.g., flattening) the radial electric field within the ICR cell. Also disclosed are methods for controlling the electric field to diminish effects of de-phasing. The diminished effects are achieved by decreasing space-charge contributions by increasing the length of the ion-oscillation path along the z-axis of the ICR cell. The methods and devices enhance the time-domain signal of a Fourier-transform ion-cyclotron resonance mass spectrometer (FTICR-MS) and provide enhanced resolution and accuracy of mass measurements.
摘要翻译：公开了具有多组捕获电极的离子回旋共振（ICR）电池和其它离子捕获电池，用于对ICR电池内的径向电场进行成形（例如，扁平化）。还公开了用于控制电场以减少去相位效应的方法。通过增加沿着ICR单元的z轴的离子振荡路径的长度来减小空间电荷的贡献来实现减小的效果。该方法和装置增强了傅立叶变换离子回旋共振质谱仪（FTICR-MS）的时域信号，并提供了更高的质量测量分辨率和精度。

6. 发明授权

US07524925B2 Protein interaction reporter agents and methods for using same 失效
标题翻译：蛋白质相互作用记录剂及其使用方法
公开(公告)号：US07524925B2
公开(公告)日：2009-04-28
申请号：US11282403
申请日：2005-11-18
申请人： James E. Bruce , Xiaoting Tang , Gerhard Munske
发明人： James E. Bruce , Xiaoting Tang , Gerhard Munske
IPC分类号： C07K2/00 , C07K1/13 , C07C229/38
CPC分类号： C12Q1/37 , C07D207/46 , C07D403/14 , C07D495/04 , G01N33/542 , G01N33/6848
摘要： Particular aspects provide novel protein interaction reporter (PIR) compounds (e.g., formulas I and II), comprising at least two protein reactive moieties (e.g., N-hydroxysuccinamide), each linked to a reporter moiety (e.g., mass reporter) by a covalent labile bond that is differentially cleavable with respect to peptide bonds (e.g., by a method such as collisional activation in a mass spectrometer, activation by electron capture dissociation (ECD), photoactivation, etc.), wherein the reporter moiety is operatively releasable from the PIR agent upon cleavage of the labile bonds, the released reporter moiety having a characteristic identifying property or label (e.g., m/z value). Particular PIRs comprise a mass reporter moiety, and further comprise an affinity group, (e.g., biotin), linked to the PIR (e.g., to the mass reporter moiety) by a selectively cleavable bone (e.g. photo-labile bond)). Additional aspects provide methods for characterizing intermolecular or intramolecular protein interactions using one or more inventive PIR compounds.
摘要翻译：特别的方面提供新的蛋白质相互作用报告物（PIR）化合物（例如式I和II），其包含至少两个蛋白质反应性部分（例如，N-羟基琥珀酰胺），其各自通过共价键连接到报道部分（例如质量报告基团）相对于肽键可以差异地切割的不稳定键（例如，通过诸如质谱仪中的碰撞激活的方法，通过电子捕获解离的激活（ECD），光活化等），其中报道部分可操作地从裂解不稳定键时的PIR试剂，具有特征识别性质或标记的释放的报导部分（例如，m / z值）。具体的PIR包括质量报告基因部分，并且还包含通过可选择性切割的骨（例如光不稳定键）与PIR连接的亲和基团（例如，生物素）（例如与质量报告基团部分））。另外的方面提供了使用一种或多种本发明的PIR化合物表征分子间或分子内蛋白质相互作用的方法。

7. 发明授权

US06196126B1 Method and apparatus for preventing pigment buildup during a rotary screen printing process 失效
标题翻译：用于防止旋转丝网印刷过程中颜料累积的方法和装置
公开(公告)号：US06196126B1
公开(公告)日：2001-03-06
申请号：US09228699
申请日：1999-01-12
申请人： Garnett H. Smith , Jerry D. Hiatt , James E. Bruce
发明人： Garnett H. Smith , Jerry D. Hiatt , James E. Bruce
IPC分类号： B41F1512
CPC分类号： B41F35/004 , B41P2235/21 , B41P2235/26 , B41P2235/40
摘要： An apparatus and method for preventing excessive pigment buildup on the exterior surface of a rotary printing screen in a region adjacent the edge of fabric being printed including a flexible wiper member biased against the exterior surface of the rotary printing screen and positioned in the excessive pigment region and a mounting bracket for holding the wiper member in a predetermined orientation with respect to the exterior surface of the rotary printing screen. The wiper member is positioned in the excessive pigment region and is angled to the axis of rotation of the rotary printing screen so as to push the excessive pigment buildup away from the fabric being printed. The method further includes applying a lubricant stream to the exterior surface adjacent to and upstream of the flexible wiper member.
摘要翻译：一种用于防止在被印刷的织物边缘附近的旋转印刷丝网的外表面上积聚过多的颜料的装置和方法，该装置和方法包括偏置在旋转印刷丝网的外表面上且位于过量颜料区域中的柔性擦拭器部件以及用于将擦拭器构件相对于旋转印刷屏幕的外表面保持在预定取向的安装支架。擦拭器构件位于过量颜料区域中并且与旋转印刷屏幕的旋转轴线成一定角度，从而将过量的颜料积聚推离出被印刷的织物。该方法还包括将润滑剂流施加到与柔性擦拭器构件相邻并上游的外表面。

8. 发明授权

US5187764A Conduit for fiber optical cables 失效
标题翻译：光纤电缆导线
公开(公告)号：US5187764A
公开(公告)日：1993-02-16
申请号：US691451
申请日：1991-04-25
申请人： Kenneth L. Hixon , James E. Bruce
发明人： Kenneth L. Hixon , James E. Bruce
IPC分类号： G02B6/44
CPC分类号： G02B6/4459 , G02B6/02033 , G02B6/4463
摘要： A conduit for receiving lengths of fiber optical cable, which enhances light emission and transmission within the cable by substantially reducing an air gap located between a polymeric core and an outer cladding of the fiber optical cable. The conduit of the present invention has a retaining member that defines a first channel adapted to receive the fiber optical cable and a base member that defines a second channel adapted to receive the cable. The retaining member is mounted with the base member, such that the cable is retained within the first and the second channels and a compressive force is exerted on the cable, which substantially eliminates the gap between the core and outer cladding. Furthermore, the conduit of the present invention supports and guides the cable and facilitates the installation thereof.
摘要翻译：用于接收长度的光纤光缆的导管，其通过基本上减少位于光纤光缆的聚合物芯和外包层之间的气隙来增强光缆内的光发射和透射。本发明的管道具有保持构件，该保持构件限定适于接收光纤光缆的第一通道和限定适于接收电缆的第二通道的基座构件。保持构件安装有基部构件，使得电缆保持在第一和第二通道内，并且压缩力施加在电缆上，这基本上消除了芯和外包层之间的间隙。此外，本发明的导管支撑并引导电缆并便于其安装。

9. 发明授权

US07858930B2 Ion-trapping devices providing shaped radial electric field 有权
标题翻译：离子捕获装置提供成形的径向电场
公开(公告)号：US07858930B2
公开(公告)日：2010-12-28
申请号：US12334259
申请日：2008-12-12
申请人： Nathan K. Kaiser , James E. Bruce
发明人： Nathan K. Kaiser , James E. Bruce
IPC分类号： H01J49/42 , B01D59/44
CPC分类号： H01J49/38
摘要： Disclosed are ion cyclotron resonance (ICR) cells and other ion-trapping cells with plural groups of multiple trapping electrodes for shaping (e.g., flattening) the radial electric field within the ICR cell. Also disclosed are methods for controlling the electric field to diminish effects of de-phasing. The diminished effects are achieved by decreasing space-charge contributions by increasing the length of the ion-oscillation path along the z-axis of the ICR cell. The methods and devices enhance the time-domain signal of a Fourier-transform ion-cyclotron resonance mass spectrometer (FTICR-MS) and provide enhanced resolution and accuracy of mass measurements.
摘要翻译：公开了具有多组捕获电极的离子回旋共振（ICR）电池和其它离子捕获电池，用于对ICR电池内的径向电场进行成形（例如，扁平化）。还公开了用于控制电场以减少去相位效应的方法。通过增加沿着ICR单元的z轴的离子振荡路径的长度来减小空间电荷的贡献来实现减小的效果。该方法和装置增强了傅立叶变换离子回旋共振质谱仪（FTICR-MS）的时域信号，并提供了更高的质量测量分辨率和精度。

10. 发明申请

US20100047814A1 PROTEIN INTERACTION REPORTER AGENTS AND METHODS FOR USING SAME 审中-公开
标题翻译：蛋白质相互作用记录剂及其使用方法
公开(公告)号：US20100047814A1
公开(公告)日：2010-02-25
申请号：US12423459
申请日：2009-04-14
申请人： James E. Bruce , Xiaoting Tang , Gerhard Munske
发明人： James E. Bruce , Xiaoting Tang , Gerhard Munske
IPC分类号： G01N33/53
CPC分类号： C12Q1/37 , C07D207/46 , C07D403/14 , C07D495/04 , G01N33/542 , G01N33/6848
摘要： Particular aspects provide novel protein interaction reporter (PIR) compounds (e.g., formulas I and II), comprising at least two protein reactive moieties (e.g., N-hydroxysuccinamide), each linked to a reporter moiety (e.g., mass reporter) by a covalent labile bond that is differentially cleavable with respect to peptide bonds (e.g., by a method such as collisional activation in a mass spectrometer, activation by electron capture dissociation (ECD), photoactivation, etc.), wherein the reporter moiety is operatively releasable from the PIR agent upon cleavage of the labile bonds, the released reporter moiety having a characteristic identifying property or label (e.g., m/z value). Particular PIRs comprise a mass reporter moiety, and further comprise an affinity group, (e.g., biotin), linked to the PIR (e.g., to the mass reporter moiety) by a selectively cleavable bone (e.g. photo-labile bond)). Additional aspects provide methods for characterizing intermolecular or intramolecular protein interactions using one or more inventive PIR compounds.
摘要翻译：特别的方面提供新的蛋白质相互作用报告物（PIR）化合物（例如式I和II），其包含至少两个蛋白质反应性部分（例如，N-羟基琥珀酰胺），其各自通过共价键连接到报道部分（例如质量报告基团）相对于肽键可以差异地切割的不稳定键（例如，通过诸如质谱仪中的碰撞激活的方法，通过电子捕获解离的激活（ECD），光活化等），其中报道部分可操作地从裂解不稳定键时的PIR试剂，具有特征识别性质或标记的释放的报导部分（例如，m / z值）。具体的PIR包括质量报告基因部分，并且还包含通过可选择性切割的骨（例如光不稳定键）与PIR连接的亲和基团（例如，生物素）（例如与质量报告基团部分））。另外的方面提供了使用一种或多种本发明的PIR化合物表征分子间或分子内蛋白质相互作用的方法。

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