专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明申请

US20060084155A1 Biological systems for manufacture of polyhydroxyalkanoate polymers containing 4-hydroxyacids 有权
公开(公告)号：US20060084155A1
公开(公告)日：2006-04-20
申请号：US11245891
申请日：2005-10-07
申请人： Gjalt Huisman , Frank Skraly , David Martin , Oliver Peoples
发明人： Gjalt Huisman , Frank Skraly , David Martin , Oliver Peoples
IPC分类号： C12P7/62 , C07H21/04 , C12N9/10 , C12N1/21 , C08G63/82 , C12N15/74
CPC分类号： C12N9/0008 , C08G63/06 , C12N9/1029 , C12N9/1096 , C12N15/8243 , C12P7/625
摘要： The gene encoding a 4-hydroxybutyryl-Co A transferase has been isolated from bacteria and integrated into the genome of bacteria also expressing a polyhydroxyalkanoate synthase, to yield an improved production process for 4HB-containing polyhydroxyalkanoates using transgenic organisms, including both bacteria and plants. The new pathways provide means for producing 4HB containing PHAs from cheap carbon sources such as sugars and fatty acids, in high yields, which are stable. Useful strains are obtaining by screening strains having integrated into their genomes a gene encoding a 4HB-CoA transferase and/or PHA synthase, for polymer production. Processes for polymer production use recombinant systems that can utilize cheap substrates. Systems are provided which can utilize amino acid degradation pathways, α-ketoglutarate, or succinate as substrate.

2. 发明申请

US20050170480A1 Transgenic microbial polyhydroxyalkanoate producers 审中-公开
公开(公告)号：US20050170480A1
公开(公告)日：2005-08-04
申请号：US11053551
申请日：2005-02-08
申请人： Gjalt Huisman , Oliver Peoples , Frank Skraly
发明人： Gjalt Huisman , Oliver Peoples , Frank Skraly
IPC分类号： C12N15/09 , C12N1/21 , C12N9/00 , C12N9/02 , C12N9/04 , C12N9/10 , C12N9/88 , C12N15/90 , C12P7/62 , C12R1/19 , C12N15/74
CPC分类号： C12N9/1029 , C12N9/00 , C12N9/0004 , C12N9/0006 , C12N9/1025 , C12N9/13 , C12N9/88 , C12N15/90 , C12P7/625 , Y10S435/829 , Y10S435/831 , Y10S435/877
摘要： Transgenic microbial strains are provided which contain the genes required for PHA formation integrated on the chromosome. The strains are advantageous in PHA production processes, because (1) no plasmids need to be maintained, generally obviating the required use of antibiotics or other stabilizing pressures, and (2) no plasmid loss occurs, thereby stabilizing the number of gene copies per cell throughout the fermentation process, resulting in homogeneous PHA product formation throughout the production process. Genes are integrated using standard techniques, preferably transposon mutagenesis. In a preferred embodiment wherein mutiple genes are incorporated, these are incorporated as an operon. Sequences are used to stabilize mRNA, to induce expression as a function of culture conditions (such as phosphate concentration), temperature, and stress, and to aid in selection, through the incorporation of selection markers such as markers conferring antibiotic resistance.

3. 发明申请

US20060183209A1 Enzymes for biopolymer production 审中-公开
标题翻译：用于生物聚合物生产的酶
公开(公告)号：US20060183209A1
公开(公告)日：2006-08-17
申请号：US11355440
申请日：2006-02-16
申请人： Oliver Peoples , Lara Madison , Gjalt Huisman
发明人： Oliver Peoples , Lara Madison , Gjalt Huisman
IPC分类号： C12N9/02 , C07H21/04 , C12N5/04
CPC分类号： C12N9/0008 , C07K2319/00 , C12N9/10 , C12N9/1025 , C12N9/1029 , C12N9/88 , C12N15/62 , C12N15/82 , C12N15/8243 , C12P7/625
摘要： In order to optimize the flux or flow of carbon intermediates from normal cellular metabolism into PHAs it is desirable to optimize the expression of the enzymes of the PHA biosynthetic pathway. Gene fusions are genetic constructs where two open reading frames have been fused into one and encode hybrid proteins and in some cases bifunctional hybrid enzymes. Linkers may be added to spatially separate the two domains of the hybrid protein. In the case of enzymes which catalyse successive reactions in a pathway, the fusion of two genes results in bringing two enzymatic activities into close proximity to each other. When the product of the first reaction is a substrate for the second one, this new configuration of active sites may result in a faster transfer of the product of the first reaction to the second active site with a potential for increasing the flux through the pathway.
摘要翻译：为了优化碳中间体从正常细胞代谢到PHAs的通量或流量，优选优化PHA生物合成途径的酶的表达。基因融合是遗传构建体，其中两个开放阅读框已融合在一起并编码杂交蛋白质，并且在某些情况下编码双功能杂合酶。可以加入接头以在空间上分离杂交蛋白的两个结构域。在催化途径中连续反应的酶的情况下，两个基因的融合导致使两个酶活性彼此靠近。当第一反应的产物是第二反应的底物时，活性位点的这种新构型可能导致第一反应的产物更快地转移到第二活性位点，具有增加通过该通路的通量的可能性。

4. 发明授权

US06703240B1 Modified starch metabolism enzymes and encoding genes for improvement and optimization of plant phenotypes 失效
标题翻译：改性淀粉代谢酶和编码基因，用于改良和优化植物表型
公开(公告)号：US06703240B1
公开(公告)日：2004-03-09
申请号：US09547844
申请日：2000-04-12
申请人： Willem P. C. Stemmer , Venkitswaran Subramanian , Sun Ai Raillard , Gjalt Huisman
发明人： Willem P. C. Stemmer , Venkitswaran Subramanian , Sun Ai Raillard , Gjalt Huisman
IPC分类号： C12N1500
CPC分类号： C08L3/02
摘要： The invention provides methods for generating, identifying, and selecting polynucleotides encoding novel starch metabolizing enzymes (NSME), NSME-encoding polynucleotides, compositions of recombinant shuffled NSME protein, plant cells and microbes containing a shuffled NSME polynucleotide in expressible form, plants containing a shuffled NSME polynucleotide in expressible form, novel starch compositions produced by plants and cells, uses of such plants, cells, and starch compositions.
摘要翻译：本发明提供了用于产生，鉴定和选择编码新型淀粉代谢酶（NSME），NSME编码多核苷酸，重组洗牌NSME蛋白质的组合物，植物细胞和含有可表达形式的洗牌NSME多核苷酸的微生物的多核苷酸的方法，所述植物含有洗牌可表达形式的NSME多核苷酸，由植物和细胞产生的新型淀粉组合物，这种植物，细胞和淀粉组合物的用途。

5. 发明授权

US08329438B2 Enone reductases 有权
标题翻译： Enone还原酶
公开(公告)号：US08329438B2
公开(公告)日：2012-12-11
申请号：US12646907
申请日：2009-12-23
申请人： Christopher Savile , Vesna Mitchell , Xiyun Zhang , Gjalt Huisman
发明人： Christopher Savile , Vesna Mitchell , Xiyun Zhang , Gjalt Huisman
IPC分类号： C12N9/02
CPC分类号： C12N9/001 , C07K2319/00 , C12N9/0036 , C12N15/00 , C12P7/24 , C12P7/26 , C12P7/52 , C12P7/62 , C12P13/002 , C12Y103/01031 , C12Y106/99001 , Y02P20/52
摘要： The disclosure relates to engineered enone reductase polypeptides having improved properties, polynucleotides encoding the engineered polypeptides, related vectors, host cells, and methods for making the engineered enone reductase polypeptides. The disclosure also provides methods of using the engineered enone reductase polypeptides for chemical transformations.
摘要翻译：本公开涉及具有改进性质的工程化烯酮还原酶多肽，编码工程改造多肽的多核苷酸，相关载体，宿主细胞和用于制备工程化烯酮还原酶多肽的方法。本公开还提供了使用工程化烯酮还原酶多肽进行化学转化的方法。

6. 发明授权

US08288141B2 Ketoreductase polypeptides for the production of 3-aryl-3-hydroxypropanamine from a 3-aryl-3-ketopropanamine 有权
标题翻译：用于从3-芳基-3-酮基丙胺生产3-芳基-3-羟基丙胺的酮还原酶多肽
公开(公告)号：US08288141B2
公开(公告)日：2012-10-16
申请号：US12549293
申请日：2009-08-27
申请人： Christopher Savile , John M. Gruber , Emily Mundorff , Gjalt Huisman , Steven James Collier
发明人： Christopher Savile , John M. Gruber , Emily Mundorff , Gjalt Huisman , Steven James Collier
IPC分类号： C12N9/02 , C12N9/04 , C12P17/12 , C12P13/00 , C07H21/04
CPC分类号： C12P17/00 , C12N9/0006 , C12P13/001 , C12Y101/01164 , C12Y101/01184
摘要： The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize a variety of chiral compounds. The engineered ketoreductase polypeptides are optimized for catalyzing the conversion of N-methyl-3-keto-3-(2-thienyl)-1-propanamine to (S)—N-methyl-3-hydroxy-3-(2-thienyl)-1-propanamine.
摘要翻译：本公开提供与天然存在的野生型酮还原酶相比具有改进性质的工程化酮还原酶。还提供了编码工程化酮还原酶的多核苷酸，能够表达工程化酮还原酶的宿主细胞，以及使用工程化酮还原酶合成各种手性化合物的方法。优化工程化的酮还原酶多肽，用于催化N-甲基-3-酮-3-（2-噻吩基）-1-丙胺向（S）-N-甲基-3-羟基-3-（2-噻吩基） -1-丙胺。

7. 发明授权

US07148054B2 Evolution of whole cells and organisms by recursive sequence recombination 有权
标题翻译：通过递归序列重组进行全细胞和生物体的进化
公开(公告)号：US07148054B2
公开(公告)日：2006-12-12
申请号：US10194686
申请日：2002-07-11
申请人： Stephen delCardayre , Matthew Tobin , Willem P. C. Stemmer , Jon E. Ness , Jeremy Minshull , Phillip Patten , Venkiteswatan Subramanian , Linda A. Castle , Claus M. Krebber , Steve Bass , Ying-Xin Zhang , Tony Cox , Gjalt Huisman , Ling Yuan , Joseph A. Affholter
发明人： Stephen delCardayre , Matthew Tobin , Willem P. C. Stemmer , Jon E. Ness , Jeremy Minshull , Phillip Patten , Venkiteswatan Subramanian , Linda A. Castle , Claus M. Krebber , Steve Bass , Ying-Xin Zhang , Tony Cox , Gjalt Huisman , Ling Yuan , Joseph A. Affholter
IPC分类号： C12N1/16 , C12N1/18
CPC分类号： C12R1/465 , A01K2267/02 , C07K14/245 , C12N15/1027 , C12N15/8271 , C12N15/8273 , C12N15/8279 , C12N15/90 , C12P17/162 , C12Q1/6811 , C12Q1/6827
摘要： The invention provides methods employing iterative cycles of recombination and selection/screening for evolution of whole cells and organisms toward acquisition of desired properties Examples of such properties include enhanced recombinogenicity, genome copy number, and capacity for expression and/or secretion of proteins and secondary metabolites.
摘要翻译：本发明提供了使用重组和选择/筛选整个细胞和生物体以获得所需性质的进化的迭代循环的方法。这些性质的实例包括增加的重组原型，基因组拷贝数和蛋白质和次级代谢物的表达和/或分泌能力。

8. 发明申请

US20050272064A1 Halohydrin dehalogenases and related polynucleotides 有权
公开(公告)号：US20050272064A1
公开(公告)日：2005-12-08
申请号：US11067323
申请日：2005-02-23
申请人： S. Davis , Richard Fox , Gjalt Huisman , Vesna Gavrilovic , Lisa Newman
发明人： S. Davis , Richard Fox , Gjalt Huisman , Vesna Gavrilovic , Lisa Newman
IPC分类号： C07H21/04 , C12N1/21 , C12N9/04 , C12N9/14 , C12N9/88 , C12N15/74 , C12Q1/68
CPC分类号： C12N9/14 , C12N9/88
摘要： The present invention relates to novel halohydrin dehalogenase polypeptides and the polynucleotides that encode them. These polypeptides are useful in the production of 4-substituted-3-butyric acid derivatives and vicinal cyano, hydroxyl substituted carboxylic acid esters. The invention also provides related vectors, host cells and methods.

9. 发明授权

US09193957B2 Engineered imine reductases and methods for the reductive animation of ketone and amine compounds 有权
标题翻译：酮和胺化合物的还原动力的工程亚胺还原酶和方法
公开(公告)号：US09193957B2
公开(公告)日：2015-11-24
申请号：US13890944
申请日：2013-05-09
申请人： Haibin Chen , Steven J. Collier , Jovana Nazor , Joly Sukumaran , Derek Smith , Jeffrey C. Moore , Gregory Hughes , Jacob Janey , Gjalt Huisman , Scott Novick , Nicholas Agard , Oscar Alvizo , Gregory Cope , Wan Lin Yeo , Stefanie Ng Minor
发明人： Haibin Chen , Steven J. Collier , Jovana Nazor , Joly Sukumaran , Derek Smith , Jeffrey C. Moore , Gregory Hughes , Jacob Janey , Gjalt Huisman , Scott Novick , Nicholas Agard , Oscar Alvizo , Gregory Cope , Wan Lin Yeo , Stefanie Ng Minor
IPC分类号： C12N9/06 , C12P13/00 , C12P13/02 , C12P13/04 , C12P13/06 , C12P17/10 , C12P17/12 , C12P17/16 , C12P17/18
CPC分类号： C12P13/02 , C12N9/0028 , C12P13/001 , C12P13/04 , C12P13/06 , C12P17/10 , C12P17/12 , C12P17/165 , C12P17/185 , C12P17/188 , C12Y105/01 , C12Y105/01023 , C12Y105/01024 , C12Y105/01028 , Y02P20/52
摘要： The present disclosure provides engineered polypeptides having imine reductase activity, polynucleotides encoding the engineered imine reductases, host cells capable of expressing the engineered imine reductases, and methods of using these engineered polypeptides with a range of ketone and amine substrate compounds to prepare secondary and tertiary amine product compounds.
摘要翻译：本公开提供了具有亚胺还原酶活性的工程改造的多肽，编码工程改造的亚胺还原酶的多核苷酸，能够表达工程化亚胺还原酶的宿主细胞，以及使用这些工程改造的多肽与一系列酮和胺底物化合物以制备仲胺和叔胺的方法产品化合物。

10. 发明授权

US08426178B2 Ketoreductase polypeptides for the production of a 3-aryl-3-hydroxypropanamine from a 3-aryl-3-ketopropanamine 有权
标题翻译：用于从3-芳基-3-酮基丙胺生产3-芳基-3-羟基丙胺的酮还原酶多肽
公开(公告)号：US08426178B2
公开(公告)日：2013-04-23
申请号：US12549154
申请日：2009-08-27
申请人： Christopher Savile , John M. Gruber , Emily Mundorff , Gjalt Huisman , Steven James Collier
发明人： Christopher Savile , John M. Gruber , Emily Mundorff , Gjalt Huisman , Steven James Collier
IPC分类号： C12N9/02 , C12P13/00 , C12Q1/26 , C07K14/00 , C12P21/00
CPC分类号： C12N9/0006 , C12P13/001 , C12P17/00 , C12P41/002
摘要： The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize a variety of chiral compounds. The engineered ketoreductase polypeptides are optimized for catalyzing the conversion of N,N-dimethyl-3-keto-3-(2-thienyl)-1-ketopropanamine to (S)—N,N-dimethyl-3-hydroxy-3-(2- thienyl)-1-propanamine.
摘要翻译：本公开提供与天然存在的野生型酮还原酶相比具有改进性质的工程化酮还原酶。还提供了编码工程化酮还原酶的多核苷酸，能够表达工程化酮还原酶的宿主细胞，以及使用工程化酮还原酶合成各种手性化合物的方法。优化了工程化酮还原酶多肽，用于催化N，N-二甲基-3-酮-3-（2-噻吩基）-1-酮基丙胺转化为（S）-N，N-二甲基-3-羟基-3-（ 2-噻吩基）-1-丙胺。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式