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    • 8. 发明授权
    • Conditional disruption of dicer1 in cell lines and non-human mammals
    • 细胞系和非人类哺乳动物中dicer1的条件破坏
    • US07582741B2
    • 2009-09-01
    • US11191157
    • 2005-07-26
    • Stephen JonesZhinqing Zhu
    • Stephen JonesZhinqing Zhu
    • C12N15/11C12N15/74
    • C12N15/8509A01K67/0276A01K2217/075A01K2227/105A01K2267/03C12N9/90C12N2800/30
    • The present invention is based, at least in part, on the generation of cells, cell lines and non-human mammals that contain a synthetic conditional allele of the Dicer1 gene. Many of the constructs, methods, animals and cells of the invention feature recombinase recognition sites (in certain embodiments, loxP sites) positioned on either flank of a sequence that comprises exons 14, 15 and 16 of the Dicer1 gene (in certain embodiments, the mouse Dicer1 gene). Through readily manipulable introduction, administration, or expression of a recombinase (in certain aspects of the invention, the Cre recombinase of bacteriophage P1), exons 14 through 16 of Dicer1 may be directedly excised, producing a functional knockout of Dicer1 in cells containing these recombinase-induced alleles of the invention.
    • 本发明至少部分地基于含有Dicer1基因的合成条件等位基因的细胞,细胞系和非人哺乳动物的产生。 本发明的许多构建体,方法,动物和细胞的特征在于位于包含Dicer1基因的外显子14,15和16的序列的任一侧的重组酶识别位点(在某些实施方案中,loxP位点)(在某些实施方案中, 小鼠Dicer1基因)。 通过易于操作的引入,施用或表达重组酶(在本发明的某些方面,噬菌体P1的Cre重组酶),Dicer1的外显子14至16可以被直接切除,在含有这些重组酶的细胞中产生Dicer1的功能性敲除 诱导的本发明的等位基因。
    • 9. 发明申请
    • CLOSED-LOOP PHYSICAL CALIPER MEASUREMENTS AND DIRECTIONAL DRILLING METHOD
    • 闭环物理卡尺测量和方向钻孔方法
    • US20090090554A1
    • 2009-04-09
    • US12332911
    • 2008-12-11
    • Junichi SugiuraStephen Jones
    • Junichi SugiuraStephen Jones
    • E21B44/00G01V9/00
    • E21B7/062E21B47/08
    • Aspects of this invention include a downhole tool and method for making a physical caliper measurement of a subterranean borehole. The tool is configured to make the physical borehole caliper measurement only when the measured pressure in each of three or more outwardly extendable blades is greater than a predetermined threshold pressure. Blade positions are measured and the borehole caliper calculated only when the pressure in each of the blades exceeds the threshold. Exemplary embodiments of the invention enable physical caliper measurements to be made with increased accuracy with each of the blades making firm contact with the borehole wall. Methods in accordance with the invention are especially well suited for use in directional drilling applications in that they tend to enable accurate caliper measurements to be made without repositioning the steering tool in the borehole.
    • 本发明的方面包括用于制造地下钻孔的物理测径仪的井下工具和方法。 只有当三个或更多个可向外延伸的叶片中的每一个中的测量压力大于预定阈值压力时,该工具被配置为仅进行物理钻孔测径器测量。 测量叶片位置,只有当每个叶片中的压力超过阈值时才计算出钻孔厚度。 本发明的示例性实施例使得能够以增加的精度进行物理测径,其中每个叶片与钻孔壁牢固接触。 根据本发明的方法特别适用于定向钻井应用,因为它们倾向于使得能够进行准确的卡尺测量,而不需要在井眼中重新定位转向工具。
    • 10. 发明授权
    • Closed-loop control of hydraulic pressure in a downhole steering tool
    • 井下转向工具中液压的闭环控制
    • US07464770B2
    • 2008-12-16
    • US11595054
    • 2006-11-09
    • Stephen JonesJunichi Sugiura
    • Stephen JonesJunichi Sugiura
    • E21B21/08E21B7/04
    • E21B7/062
    • Aspects of this invention include a steering tool having a controller configured to provide closed-loop control of hydraulic fluid pressure. In one exemplary embodiment, closed-loop control of a system (reservoir) pressure may be provided. In another embodiment, closed-loop control of a blade pressure may be provided while the blade remains substantially locked at a predetermined position. Other exemplary embodiments may incorporate rule-based-intelligence such that pressure control thresholds may be determined based on various measured and/or predetermined downhole parameters. The invention tends to reduce the friction (drag) between the blades and the borehole wall and thereby also tends to improve drilling rates. Moreover, the invention also tends to improve the service life and reliability of downhole steering tools.
    • 本发明的方面包括具有被配置为提供液压流体压力的闭环控制的控制器的转向工具。 在一个示例性实施例中,可以提供系统(储存器)压力的闭环控制。 在另一个实施例中,可以在叶片保持基本上锁定在预定位置的同时提供叶片压力的闭环控制。 其他示例性实施例可以包括基于规则的智能,使得可以基于各种测量和/或预定的井下参数来确定压力控制阈值。 本发明倾向于减小叶片与钻孔壁之间的摩擦(阻力),从而也提高钻井速率。 此外,本发明还倾向于提高井下转向工具的使用寿命和可靠性。