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    • 1. 发明授权
    • Expression of fructose 1,6 bisphosphate aldolase in transgenic plants
    • 果糖1,6-二磷酸醛缩酶在转基因植物中的表达
    • US06716474B2
    • 2004-04-06
    • US09923109
    • 2001-08-06
    • Gerard F. BarryNordine CheikhGanesh M. Kishore
    • Gerard F. BarryNordine CheikhGanesh M. Kishore
    • A23L1217
    • C12N9/88C12N15/8245C12N15/8261Y02A40/146
    • Fructose-1,6-bisphosphate aldolase (FDA) is an enzyme reversibly catalyzing the reaction converting triosephosphate into fructose-1,6-bisphosphate. In the leaf, this enzyme is located in the chloroplast (starch synthesis) and the cytosol (sucrose biosynthesis). Transgenic plants were generated that express the E. coli fda gene in the chloroplast to improve plant yield by increasing leaf starch biosynthetic ability in particular and sucrose production in general. Leaves from plants expressing the fda transgene showed a significantly higher starch accumulation, as compared to control plants expressing the null vector, particularly early in the photoperiod, but had lower leaf sucrose. Transgenic plants also had a significantly higher root mass. Furthermore, transgenic potatoes expressing fda exhibited improved uniformity of solids.
    • 果糖-1,6-二磷酸醛缩酶(FDA)是一种可逆地催化将磷酸三脂转化为果糖-1,6-二磷酸的反应的酶。 在叶中,该酶位于叶绿体(淀粉合成)和胞质溶胶(蔗糖生物合成)中。 产生转基因植物,其通过增加叶淀粉生物合成能力和蔗糖生产通常在叶绿体中表达大肠杆菌fda基因以提高植物产量。 与表达无载体的对照植物相比,表达fda转基因植物的叶子显示出显着更高的淀粉积累,特别是在光周期的早期,但具有较低的叶片蔗糖。 转基因植物也具有显着更高的根质量。 此外,表达fda的转基因马铃薯表现出改善的固体均匀性。