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1. 发明授权

US08420352B2 Protein delivery system to generate pluripotent stem (iPS) cells or tissue specific cells 有权
标题翻译：产生多能干细胞或组织特异性细胞的蛋白质递送系统
公开(公告)号：US08420352B2
公开(公告)日：2013-04-16
申请号：US12870782
申请日：2010-08-27
申请人： George A. Oyler , Yung-Nien Chang
发明人： George A. Oyler , Yung-Nien Chang
IPC分类号： C07K14/33 , C12N1/20 , C12N15/62 , C12P21/02
CPC分类号： C12N5/0696 , A61K35/74 , A61K38/164 , C07K14/33 , C07K14/4702 , C07K2319/00 , C07K2319/10 , C07K2319/55 , C12N5/00 , C12N2501/602 , C12N2501/603
摘要： A novel protein delivery system to generate induced pluripotent stem (iPS) cells is described. The delivery system comprises a construct with a receptor binding domain that recognizes a receptor in a somatic cell, a translocation domain that allows the transfer of an inducer into the cytosolic space, and a cargo bearing domain to which the inducer is attached and facilitates transfer of the inducer into the cell.
摘要翻译：描述了产生诱导多能干细胞（iPS）细胞的新型蛋白质递送系统。递送系统包括具有受体结合结构域的构建体，其识别体细胞中的受体，允许诱导物转移到细胞溶质空间中的易位结构域，以及引诱物附着的携带轴承结构域，并促进诱导剂进入细胞。

2. 发明申请

US20140004605A1 METHOD FOR EXTRACTION AND PURIFICATION OF OILS FROM MICROALGAL BIOMASS USING HIGH-PRESSURE CO2 AS A SOLUTE 审中-公开
标题翻译：使用高压二氧化碳作为溶剂从微生物生物质中提取和纯化油的方法
公开(公告)号：US20140004605A1
公开(公告)日：2014-01-02
申请号：US13816737
申请日：2011-08-16
申请人： Marc D. Donohue , Michael J. Betenbaugh , George A. Oyler , Julian N. Rosenberg
发明人： Marc D. Donohue , Michael J. Betenbaugh , George A. Oyler , Julian N. Rosenberg
IPC分类号： C11B1/10
CPC分类号： C11B1/10 , C10G2300/1011 , C10G2300/1014 , C10G2300/4081 , C10L1/02 , C11B3/02 , Y02P30/20
摘要： The present invention provides methods for the isolation of oils from intact or lysed microorganisms in aqueous media with pressurized carbon dioxide as a solute. Such oils may be used for the production of biofuels. Also provided for are methods for harvesting and rupturing whole cell microorganisms in aqueous media with pressurized carbon dioxide as a solute
摘要翻译：本发明提供了在具有加压二氧化碳作为溶质的水性介质中从完整或裂解的微生物分离油的方法。这种油可用于生产生物燃料。还提供了用加压二氧化碳作为溶质在水性介质中收获和破裂全细胞微生物的方法

3. 发明授权

US08343743B2 Designer ubiquitin ligases having a non-cleavable SNAP25 domain and E3-ligase domain 失效
标题翻译：具有不可切割的SNAP25结构域和E3连接酶结构域的设计者泛素连接酶
公开(公告)号：US08343743B2
公开(公告)日：2013-01-01
申请号：US12482420
申请日：2009-06-10
申请人： George A. Oyler , Yien Che Tsai
发明人： George A. Oyler , Yien Che Tsai
IPC分类号： C12N9/00 , C12N1/20 , C12N15/00 , C07H21/04 , A61K38/43
CPC分类号： C12N9/93 , A61K38/4886 , Y02A50/471
摘要： The present invention relates to a designer or recombinant ubiquitin ligase molecule that includes a toxin binding domain that is specific for a toxin active fragment, wherein the toxin active fragment is an enzymatically active fragment of one or more toxins or toxin serotypes; and an E3-ligase domain that comprises an E3-ligase or polypeptide that facilitates E2-mediated ubiquitination of the toxin active fragment. In an embodiment, the composition further includes a delivery system that allow the designer ubiquitin ligase to enter the cell. The present invention further includes methods for treating an individual intoxicated with a toxin by administering the designer ubiquitin ligase of the present invention.
摘要翻译：本发明涉及一种设计者或重组泛素连接酶分子，其包括对毒素活性片段特异的毒素结合结构域，其中所述毒素活性片段是一种或多种毒素或毒素血清型的酶活性片段; 和E3连接酶结构域，其包含促进E2介导的毒素活性片段的泛素化的E3连接酶或多肽。在一个实施方案中，组合物还包括允许设计者泛素连接酶进入细胞的递送系统。本发明还包括通过施用本发明的设计者泛素连接酶来治疗用毒素中毒的个体的方法。

4. 发明申请

US20120277411A1 SINGLE CHAIN ANTIBODIES FOR PHOTOSYNTHETIC MICROORGANISMS AND METHODS OF USE 审中-公开
标题翻译：用于光合作用微生物的单链抗体及其使用方法
公开(公告)号：US20120277411A1
公开(公告)日：2012-11-01
申请号：US13441951
申请日：2012-04-09
申请人： George A. Oyler , Julian N. Rosenberg , Donald P. Weeks
发明人： George A. Oyler , Julian N. Rosenberg , Donald P. Weeks
IPC分类号： C07K14/405 , C07K1/14 , C07K19/00
CPC分类号： C07K16/14 , C07K2317/22 , C07K2317/569 , C07K2319/00 , C07K2319/20 , C07K2319/70 , C12N1/02 , C12N1/12 , C12N1/22
摘要： A single chain antibody that binds algae is described. The single chain antibody for algae is used to capture algae onto bioactive films. The single chain antibody is also used in a chimeric construct having a substrate binding domain and a single chain antibody domain. Dimers, trimmers, and multimer constructs are also described that aid in collection of algae from liquid mixtures by causing flocculation of algae cells.
摘要翻译：描述了结合藻类的单链抗体。用于藻类的单链抗体用于将藻类捕获到生物活性膜上。单链抗体也用于具有底物结合结构域和单链抗体结构域的嵌合构建体中。还描述了二聚体，微调剂和多聚体构建体，其通过引起藻类细胞的絮凝来帮助从液体混合物中收集藻类。

5. 发明申请

US20100278826A1 Designer Ubiquitin Ligases For Regulation Of Intracellular Pathogenic Proteins 审中-公开
标题翻译：设计人员用于调节细胞内病原性蛋白质的泛素连接酶
公开(公告)号：US20100278826A1
公开(公告)日：2010-11-04
申请号：US12481889
申请日：2009-06-10
申请人： Charles B. Shoemaker , George A. Oyler , Saul Tzipori
发明人： Charles B. Shoemaker , George A. Oyler , Saul Tzipori
IPC分类号： A61K39/395 , C12N9/00 , C07K16/18 , C07H21/04 , C12N5/071 , C12N15/79 , C07K14/00 , A61P39/00
CPC分类号： C12N9/93 , A61K38/00 , C07K16/1282 , C07K2317/22 , C07K2317/569 , C07K2317/622 , C07K2319/00 , C07K2319/55 , C07K2319/95 , C12N9/104 , C12Y603/02019
摘要： The present invention relates to a designer or recombinant ubiquitin ligase molecule that includes an antibody fragment that is specific for a toxin active fragment, wherein the toxin active fragment is an enzymatically active fragment of one or more toxins or toxin serotypes; and an E3-ligase domain that comprises an E3-ligase or polypeptide that facilitates E2-mediated ubiquitination of the toxin active fragment. In an embodiment, the composition further includes a delivery system that allow the designer ubiquitin ligase to enter the cell. The present invention further includes methods for treating an individual intoxicated with a toxin by administering the designer ubiquitin ligase of the present invention.
摘要翻译：本发明涉及设计者或重组泛素连接酶分子，其包括对毒素活性片段特异的抗体片段，其中所述毒素活性片段是一种或多种毒素或毒素血清型的酶促活性片段; 和E3连接酶结构域，其包含促进E2介导的毒素活性片段的泛素化的E3连接酶或多肽。在一个实施方案中，组合物还包括允许设计者泛素连接酶进入细胞的递送系统。本发明还包括通过施用本发明的设计者泛素连接酶来治疗用毒素中毒的个体的方法。

6. 发明授权

US09359580B2 Method for extraction and purification of oils from microalgal biomass using high-pressure CO2 as a solute 有权
标题翻译：使用高压二氧化碳作为溶质从微藻生物质中提取和纯化油的方法
公开(公告)号：US09359580B2
公开(公告)日：2016-06-07
申请号：US13816737
申请日：2011-08-16
申请人： Marc D. Donohue , Michael J. Betenbaugh , George A. Oyler , Julian N. Rosenberg
发明人： Marc D. Donohue , Michael J. Betenbaugh , George A. Oyler , Julian N. Rosenberg
IPC分类号： B01D11/00 , C11B1/10 , C11B3/10 , C10L1/02 , C11B3/02
CPC分类号： C11B1/10 , C10G2300/1011 , C10G2300/1014 , C10G2300/4081 , C10L1/02 , C11B3/02 , Y02P30/20
摘要： The present invention provides methods for the isolation of oils from intact or lysed microorganisms in aqueous media with pressurized carbon dioxide as a solute. Such oils may be used for the production of biofuels. Also provided for are methods for harvesting and rupturing whole cell microorganisms in aqueous media with pressurized carbon dioxide as a solute.
摘要翻译：本发明提供了在具有加压二氧化碳作为溶质的水性介质中从完整或裂解的微生物分离油的方法。这种油可用于生产生物燃料。还提供了用加压二氧化碳作为溶质在水性介质中收获和破裂全细胞微生物的方法。

7. 发明授权

US08940482B1 N-end rule protease activity indication methods and uses thereof 有权
标题翻译： N端规则蛋白酶活性指示方法及其用途
公开(公告)号：US08940482B1
公开(公告)日：2015-01-27
申请号：US13159284
申请日：2011-06-13
申请人： George A. Oyler , Yien Che Tsai
发明人： George A. Oyler , Yien Che Tsai
IPC分类号： C12Q1/37
CPC分类号： C12Q1/66 , C12Q1/37 , G01N2333/33 , G01N2333/952 , G01N2500/10
摘要： A cell based assay for detection for protease activity is disclosed. In the assay a cell is engineered to express a protease substrate with at least one label, preferably on its C-terminus. Cleavage of the substrate by the protease that recognizes it results in a C-terminal fragment and a N-terminal fragment, where the fragment having the label is subject to ubiquitin proteasome degradation. The assay measures the disappearance of the label due to degradation of the fragment to which it is attached. A cell free assay is also described for detection of protease activity. In the cell free assay, the protease substrate is expressed in a solution that includes the elements of the ubiquitin proteasome pathway for degradation of the fragment. The assay measures the disappearance of the label attached to the fragment that results from cleavage by the protease.
摘要翻译：公开了一种用于蛋白酶活性检测的基于细胞的测定法。在测定中，将细胞工程化以表达具有至少一个标记的蛋白质底物，优选在其C-末端。通过识别它的蛋白酶切割底物导致C-末端片段和N-末端片段，其中具有标记的片段经受泛素蛋白酶体降解。该测定法测量标签由于其附着的片段的降解而消失。还描述了无细胞测定法用于检测蛋白酶活性。在无细胞测定中，蛋白酶底物在包含用于降解片段的泛素蛋白酶体途径的元件的溶液中表达。该测定法测量由蛋白酶切割导致的片段附着的标记物的消失。

8. 发明授权

US08492109B2 Methods for the delivery of toxins or enzymatically active portions thereof 有权
公开(公告)号：US08492109B2
公开(公告)日：2013-07-23
申请号：US12690427
申请日：2010-01-20
申请人： George A. Oyler , Charles B. Shoemaker , Chuehling Kuo
发明人： George A. Oyler , Charles B. Shoemaker , Chuehling Kuo
IPC分类号： C12Q1/37 , C12Q1/02 , C12N5/07 , C12N15/00
CPC分类号： C12N5/0618 , C12N5/0686 , C12N9/52 , C12Y304/24069 , G01N33/5014 , G01N2333/21 , G01N2333/25 , G01N2333/28 , G01N2333/32 , G01N2333/33 , G01N2333/415 , G01N2333/43517 , G01N2333/705
摘要： The present invention relates to methods, systems, and kits for intoxicating cells, neuronal and non-neuronal cells, with a toxin or fragment thereof. This is done by subjecting toxin substrate and a lipid or polymeric carrier (e.g., DNA uptake facilitating agent) to one or more cells for use in cell based assays. In an aspect, the methods of the present invention allow for high throughput assays and, as such, for the evaluation of drug candidates.

9. 发明申请

US20130065312A1 METHOD AND COMPOSITION FOR GENERATING PROGRAMMED CELL DEATH RESISTANT ALGAL CELLS 审中-公开
标题翻译：用于产生编程性细胞死亡细胞的方法和组合物
公开(公告)号：US20130065312A1
公开(公告)日：2013-03-14
申请号：US13505783
申请日：2010-11-03
申请人： Michael J. Betenbaugh , George A. Oyler , Julian N. Rosenberg
发明人： Michael J. Betenbaugh , George A. Oyler , Julian N. Rosenberg
IPC分类号： C12N1/13 , C12N15/79
CPC分类号： C12N15/8263 , A01G33/00 , C07K14/4747 , C12N15/8261 , Y02A40/146 , Y02A40/88
摘要： The present invention provides transgenic algal cells resistant to programmed cell death (PCD) and methods and compositions useful in generating such cells. Specifically, the invention utilizes expression of one or more mammalian anti-apoptotic genes in algal cells to promote resistance to PCD, which is useful for stress tolerance and increased cell viability and biomass production during cultivation.
摘要翻译：本发明提供对程序性细胞死亡（PCD）有抗性的转基因藻类细胞以及可用于产生这种细胞的方法和组合物。具体地，本发明利用藻类细胞中一种或多种哺乳动物抗凋亡基因的表达来促进对PCD的抗性，其对于胁迫耐受性和培养期间增加的细胞活力和生物量产生是有用的。

10. 发明申请

US20150010931A1 N-END RULE PROTEASE ACTIVITY INDICATION METHODS AND USES THEREOF 有权
标题翻译： N末端规则蛋白酶活性指示方法及其用途
公开(公告)号：US20150010931A1
公开(公告)日：2015-01-08
申请号：US13159284
申请日：2011-06-13
申请人： George A. Oyler , Yien Che Tsai
发明人： George A. Oyler , Yien Che Tsai
IPC分类号： C12Q1/66 , C12Q1/37
CPC分类号： C12Q1/66 , C12Q1/37 , G01N2333/33 , G01N2333/952 , G01N2500/10
摘要： A cell based assay for detection for protease activity is disclosed. In the assay a cell is engineered to express a protease substrate with at least one label, preferably on its C-terminus. Cleavage of the substrate by the protease that recognizes it results in a C-terminal fragment and a N-terminal fragment, where the fragment having the label is subject to ubiquitin proteasome degradation. The assay measures the disappearance of the label due to degradation of the fragment to which it is attached. A cell free assay is also described for detection of protease activity. In the cell free assay, the protease substrate is expressed in a solution that includes the elements of the ubiquitin proteasome pathway for degradation of the fragment. The assay measures the disappearance of the label attached to the fragment that results from cleavage by the protease.
摘要翻译：公开了一种用于蛋白酶活性检测的基于细胞的测定法。在测定中，将细胞工程化以表达具有至少一个标记的蛋白质底物，优选在其C-末端。通过识别它的蛋白酶切割底物导致C-末端片段和N-末端片段，其中具有标记的片段经受泛素蛋白酶体降解。该测定法测量标签由于其附着的片段的降解而消失。还描述了无细胞测定法用于检测蛋白酶活性。在无细胞测定中，蛋白酶底物在包含用于降解片段的泛素蛋白酶体途径的元件的溶液中表达。该测定法测量由蛋白酶切割导致的片段附着的标记物的消失。

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