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1. 发明申请

WO2013036764A1 EUKARYOTIC ORGANISMS AND METHODS FOR PRODUCING 1,3-BUTANEDIOL 审中-公开
标题翻译：用于生产1,3-丁二醇的原始有机体和方法
公开(公告)号：WO2013036764A1
公开(公告)日：2013-03-14
申请号：PCT/US2012/054152
申请日：2012-09-07
申请人： GENOMATICA, INC , BURGARD, Anthony, P. , BURK, Mark, J. , OSTERHOUT, Robin, E. , PHARKYA, Priti , LI, Jingyi
发明人： BURGARD, Anthony, P. , BURK, Mark, J. , OSTERHOUT, Robin, E. , PHARKYA, Priti , LI, Jingyi
IPC分类号： C12P7/04 , C12P1/00
CPC分类号： C12N15/52 , C08G63/16 , C12N1/14 , C12N1/16 , C12N1/20 , C12P7/18 , C12P19/32
摘要： Provided herein are non-naturally occurring eukaryotic organisms that can be engineered to produce and increase the availability of cytosolic acetyl-CoA. Also provided herein are non-naturally occurring eukaryotic organisms having a 1,3-butanediol (1,3-BDO) pathway, and methods of using such organisms to produce 1,3-BDO.
摘要翻译：本文提供了非天然存在的真核生物，其可被工程化以产生和增加细胞溶质乙酰辅酶A的可用性。本文还提供具有1,3-丁二醇（1,3-BDO）途径的非天然存在的真核生物，以及使用这些生物体产生1,3-BDO的方法。

2. 发明申请

WO2021108617A1 ENGINEERED CELLS FOR PRODUCTION OF CANNABINOIDS AND OTHER MALONYL-CoA-DERIVED PRODUCTS 审中-公开
公开(公告)号：WO2021108617A1
公开(公告)日：2021-06-03
申请号：PCT/US2020/062308
申请日：2020-11-25
申请人： GENOMATICA, INC.
发明人： LI, Jingyi , DEWALS, Pierre , SCHIRMER, Andreas , GHATAK, Sankha , GEORGIANNA, David R.
IPC分类号： C12N15/52 , C12N9/00 , C12N15/82 , C12N9/90 , C12P17/06
摘要： The invention relates to engineered microorganisms (e.g., E. coli) and associated improvements for increasing the production cannabinoids (e.g. CBGA) or precursors or derivatives thereof.

3. 发明申请

WO2020160289A1 ENGINEERED CELLS FOR IMPROVED PRODUCTION OF CANNABINOIDS 审中-公开
公开(公告)号：WO2020160289A1
公开(公告)日：2020-08-06
申请号：PCT/US2020/015913
申请日：2020-01-30
申请人： GENOMATICA, INC.
发明人： NOBLE, Michael A. , MOORE, Jonathan , LI, Jingyi , LECHNER, Anna
IPC分类号： C12N15/52 , C12N15/70 , C12P7/22
摘要： The invention provides non-natural microbial organisms containing enzymatic pathways and/or metabolic modifications for enhancing synthesis of olivetolic acid, olivetolic acid derivatives and/or cannabinoids.

4. 发明申请

WO2017075208A1 METHANOL DEHYDROGENASE FUSION PROTEINS 审中-公开
标题翻译：甲醇脱氢酶融合蛋白
公开(公告)号：WO2017075208A1
公开(公告)日：2017-05-04
申请号：PCT/US2016/059096
申请日：2016-10-27
申请人： GENOMATICA, INC.
发明人： BARTON, Nelson R. , LI, Jingyi , WARNER, Joseph R. , PHARKYA, Priti
IPC分类号： C07K14/195 , C12N15/62 , C12N5/00
CPC分类号： C12Y503/01027 , C07K2319/00 , C12N9/0006 , C12N9/88 , C12N9/90 , C12N15/52 , C12Y401/02043
摘要： Described herein are fusion proteins including methanol dehydrogenase (MeDH) and at least one other polypeptide such as 3-hexulose-6-phosphate dehydrogenase (HPS) or 6-phospho-3-hexuloisomerase (PHI), such as DHAS synthase or fructose-6-Phosphate aldolase or such as DHA synthase or DHA kinase. In a localized manner, the fusion protein can promote the conversion of methanol to formaldehyde and then to a ketose phosphate such as hexulose 6-phosphate or then to DHA and G3P. When expressed in cells, the fusion proteins can promote methanol uptake and rapid conversion to the ketose phosphate or to the DHA and D3P, which in turn can be used in a pathway for the production of a desired bioproduct. Beneficially, the rapid conversion to the ketose phosphate or to the DHA and G3P can avoid the undesirable accumulation of formaldehyde in the cell. Also described are engineered cells expressing the fusion protein, optionally include one or more additional metabolic pathway transgene(s), methanol metabolic pathway genes, target product pathway genes, cell culture compositions including the cells, methods for promoting production of the target product or intermediate thereof from the cells, compositions including the target product or intermediate, and products made from the target product or intermediate.
摘要翻译：本文描述了融合蛋白，其包括甲醇脱氢酶（MeDH）和至少一种其他多肽如3-己酮糖-6-磷酸脱氢酶（HPS）或6-磷酸-3-己酮糖异构酶（PHI），如DHAS合酶或果糖-6-磷酸醛缩酶或例如DHA合酶或DHA激酶。以局部方式，融合蛋白可以促进甲醇转化为甲醛，然后转化为磷酸酮糖如己酮糖6-磷酸，然后转化为DHA和G3P。当在细胞中表达时，融合蛋白可以促进甲醇吸收并快速转化为磷酸酮糖或DHA和D3P，其又可用于生产期望的生物产品的途径中。有利的是，快速转化为磷酸酮糖或DHA和G3P可以避免细胞中不希望的甲醛累积。还描述了表达融合蛋白的工程细胞，任选地包括一种或多种另外的代谢途径转基因，甲醇代谢途径基因，靶产物途径基因，包括细胞的细胞培养组合物，用于促进目标产物或中间产物生成的方法包括目标产物或中间体的组合物，以及由目标产物或中间体制备的产物。

5. 发明申请

WO2015051298A2 ALCOHOL DEHYDROGENASE VARIANTS 审中-公开
标题翻译：酒精脱水变异
公开(公告)号：WO2015051298A2
公开(公告)日：2015-04-09
申请号：PCT/US2014/059135
申请日：2014-10-03
申请人： GENOMATICA, INC.
发明人： ANDRAE, Stefan , KUCHINSKAS, Michael Patrick , LI, Jingyi , NAGARAJAN, Harish , PHARKYA, Priti
IPC分类号： C12P7/16 , C12N1/21
CPC分类号： C12N9/0006 , C12P7/18 , C12P7/24 , C12Y101/01001 , C12Y101/01244 , Y02P20/127
摘要： Described herein are non-natural NAD+-dependent alcohol dehydrogenases (ADHs) capable of at least two fold greater conversion of methanol or ethanol to formaldehyde or acetaldehyde, respectively, as compared to its unmodified counterpart. Nucleic acids encoding the non-natural alcohol dehydrogenases, as well as expression constructs including the nucleic acids, and engineered cells comprising the nucleic acids or expression constructs are described. Also described are engineered cells expressing a non-natural NAD + -dependent alcohol dehydrogenase, optionally include one or more additional metabolic pathway transgene(s), methanol metabolic pathway genes, target product pathway genes, cell culture compositions including the cells, methods for promoting production of the target product or intermediate thereof from the cells, compositions including the target product or intermediate, and products made from the target product or intermediate.
摘要翻译：本文描述了与其未修饰的对应物相比，能够分别甲醇或乙醇转化为甲醛或乙醛的至少两倍的非天然NAD +依赖性醇脱氢酶（ADH）。描述了编码非天然醇脱氢酶的核酸，以及包含核酸的表达构建体和包含核酸或表达构建体的工程化细胞。还描述了表达非天然NAD +依赖性醇脱氢酶的工程细胞，任选地包括一种或多种另外的代谢途径转基因，甲醇代谢途径基因，靶产物途径基因，包括细胞的细胞培养组合物，促进生产的方法的靶产物或其中间体，包括靶产物或中间体的组合物，以及由目标产物或中间体制成的产品。

6. 发明公开

EP2753699A1 EUKARYOTIC ORGANISMS AND METHODS FOR PRODUCING 1,3-BUTANEDIOL 审中-公开
标题翻译：真核生物及其制备方法1,3-丁二醇
公开(公告)号：EP2753699A1
公开(公告)日：2014-07-16
申请号：EP12829409.7
申请日：2012-09-07
申请人： Genomatica, Inc.
发明人： BURGARD, Anthony, P. , BURK, Mark, J. , OSTERHOUT, Robin, E. , PHARKYA, Priti , LI, Jingyi
IPC分类号： C12P7/04 , C12P1/00
CPC分类号： C12N15/52 , C08G63/16 , C12N1/14 , C12N1/16 , C12N1/20 , C12P7/18 , C12P19/32
摘要： Provided herein are non-naturally occurring eukaryotic organisms that can be engineered to produce and increase the availability of cytosolic acetyl-CoA. Also provided herein are non-naturally occurring eukaryotic organisms having a 1,3-butanediol (1,3-BDO) pathway. and methods of using such organisms to produce 1,3-BDO.

7. 发明公开

EP3052635A2 ALCOHOL DEHYDROGENASE VARIANTS 审中-公开
标题翻译：醇脱氢酶变体
公开(公告)号：EP3052635A2
公开(公告)日：2016-08-10
申请号：EP14850119.0
申请日：2014-10-03
申请人： Genomatica, Inc.
发明人： ANDRAE, Stefan , KUCHINSKAS, Michael Patrick , LI, Jingyi , NAGARAJAN, Harish , PHARKYA, Priti
IPC分类号： C12P1/00 , C12P5/00
CPC分类号： C12N9/0006 , C12P7/18 , C12P7/24 , C12Y101/01001 , C12Y101/01244 , Y02P20/127
摘要： Described herein are non-natural NAD+-dependent alcohol dehydrogenases (ADHs) capable of at least two fold greater conversion of methanol or ethanol to formaldehyde or acetaldehyde, respectively, as compared to its unmodified counterpart. Nucleic acids encoding the non-natural alcohol dehydrogenases, as well as expression constructs including the nucleic acids, and engineered cells comprising the nucleic acids or expression constructs are described. Also described are engineered cells expressing a non-natural NAD
+ -dependent alcohol dehydrogenase, optionally include one or more additional metabolic pathway transgene(s), methanol metabolic pathway genes, target product pathway genes, cell culture compositions including the cells, methods for promoting production of the target product or intermediate thereof from the cells, compositions including the target product or intermediate, and products made from the target product or intermediate.
摘要翻译：与未修饰的对应物相比，本文描述的是非天然NAD +依赖性醇脱氢酶（ADH），其分别能够将甲醇或乙醇分别转化为甲醛或乙醛的两倍以上。描述了编码非天然醇脱氢酶的核酸，以及包括核酸的表达构建体，以及包含核酸或表达构建体的工程细胞。还描述了表达非天然NAD +依赖性醇脱氢酶的工程细胞，任选地包括一种或多种另外的代谢途径转基因，甲醇代谢途径基因，靶产物途径基因，包括细胞的细胞培养组合物，用于促进从细胞生产目标产物或其中间体，包括目标产物或中间体的组合物，以及由目标产物或中间体制备的产物。

8. 发明申请

WO2020214951A1 OLIVETOL SYNTHASE VARIANTS AND METHODS FOR PRODUCTION OF OLIVETOLIC ACID AND ITS ANALOG COMPOUNDS 审中-公开
公开(公告)号：WO2020214951A1
公开(公告)日：2020-10-22
申请号：PCT/US2020/028766
申请日：2020-04-17
申请人： GENOMATICA, INC.
发明人： NOBLE, Michael A. , KOMOR, Russell S. , LI, Jingyi
IPC分类号： C12P7/22 , C12N15/54 , C12N15/63 , C12P1/04 , C12P7/00 , C12P7/40 , C12P7/42 , C12N1/15 , C12N5/04
摘要： Described herein are non-natural olivetol synthase (OLS) variants, nucleic acids, engineered cells, method s for preparing cannabinoids, and compositions thereof. The non-natural olivetol OLS variants form desired cannabinoid precursor and products at increased rates, have higher affinity for pathway substrates, and/or byproducts are formed in lower amounts in their presence, as compared to wild type OLS. The OLS variants can be used to form linear polyketides, and can be expressed in an engineered cell having a pathway to form cannabinoids, which include CBGA, its analogs and derivatives. CBGA can be used for the preparation of cannabigerol (CBG), which can be used in therapeutic compositions.

9. 发明申请

WO2013071226A1 EUKARYOTIC ORGANISMS AND METHODS FOR INCREASING THE AVAILABILITY OF CYTOSOLIC ACETYL-COA, AND FOR PRODUCING 1,3-BUTANEDIOL 审中-公开
标题翻译：用于增加CYPOSOLIC ACETYL-COA和生产1,3-丁二醇的可用性的原始有机体和方法
公开(公告)号：WO2013071226A1
公开(公告)日：2013-05-16
申请号：PCT/US2012/064647
申请日：2012-11-12
申请人： GENOMATICA, INC.
发明人： BURGARD, Anthony, P. , BURK, Mark, J. , OSTERHOUT, Robin, E. , PHARKYA, Priti , LI, Jingyi
IPC分类号： C12N1/20 , C12P7/16
CPC分类号： C12P7/18 , C12N15/52 , C12P7/16 , C12P19/32 , Y02E50/10
摘要： Provided herein are non-naturally occurring eukaryotic organisms that can be engineered to produce and increase the availability of cytosolic acetyl-CoA. Also provided herein are non- naturally occurring eukaryotic organisms having a 1,3-butanediol (1,3-BDO) pathway, and methods of using such organisms to produce 1,3-BDO.
摘要翻译：本文提供的是非天然存在的真核生物，其可被工程化以产生和增加细胞溶质乙酰辅酶A的可用性。本文还提供了具有1,3-丁二醇（1,3-BDO）途径的非天然存在的真核生物，以及使用这些生物体产生1,3-BDO的方法。

10. 发明公开

EP3368548A1 METHANOL DEHYDROGENASE FUSION PROTEINS 有权
公开(公告)号：EP3368548A1
公开(公告)日：2018-09-05
申请号：EP16791508.1
申请日：2016-10-27
申请人： Genomatica, Inc.
发明人： BARTON, Nelson R. , LI, Jingyi , WARNER, Joseph R. , PHARKYA, Priti
IPC分类号： C07K14/195 , C12N15/62 , C12N5/00
CPC分类号： C12Y503/01027 , C07K2319/00 , C12N9/0006 , C12N9/88 , C12N9/90 , C12N15/52 , C12Y401/02043
摘要： Described herein are fusion proteins including methanol dehydrogenase (MeDH) and at least one other polypeptide such as 3-hexulose-6-phosphate dehydrogenase (HPS) or 6-phospho-3-hexuloisomerase (PHI), such as DHAS synthase or fructose-6-Phosphate aldolase or such as DHA synthase or DHA kinase. In a localized manner, the fusion protein can promote the conversion of methanol to formaldehyde and then to a ketose phosphate such as hexulose 6-phosphate or then to DHA and G3P. When expressed in cells, the fusion proteins can promote methanol uptake and rapid conversion to the ketose phosphate or to the DHA and D3P, which in turn can be used in a pathway for the production of a desired bioproduct. Beneficially, the rapid conversion to the ketose phosphate or to the DHA and G3P can avoid the undesirable accumulation of formaldehyde in the cell. Also described are engineered cells expressing the fusion protein, optionally include one or more additional metabolic pathway transgene(s), methanol metabolic pathway genes, target product pathway genes, cell culture compositions including the cells, methods for promoting production of the target product or intermediate thereof from the cells, compositions including the target product or intermediate, and products made from the target product or intermediate.

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