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1. 发明授权

US07083967B1 Cyanobacterial nucleic acid fragments encoding proteins useful for controlling plant traits via nuclear or plastome transformation 失效
标题翻译：编码可用于通过核或plastome转化来控制植物性状的蛋白质的蓝细菌核酸片段
公开(公告)号：US07083967B1
公开(公告)日：2006-08-01
申请号：US09893033
申请日：2001-06-27
申请人： Genichi Kakefuda , Hans Koop , Stephen Sturner , Rui-Guang Zhen
发明人： Genichi Kakefuda , Hans Koop , Stephen Sturner , Rui-Guang Zhen
IPC分类号： C12N9/88 , C12N1/20 , C12N15/00 , C07H21/04 , A01H1/00
CPC分类号： C12N15/8278 , C12N9/0004 , C12N9/88 , C12Y202/01006
摘要： This invention provides an alternative source of ahas and pds nucleic acids for plant transformation and selection. In particular, the invention provides ahas and pds nucleic acids from cyanobacteria, for example, Synechocystis, and expression elements of these genes for control of expression in plastids. The invention further provides nucleic acids encoding the acetolactate synthase (ahas) large subunit and the ahas small subunit which were found to provide herbicide resistance to plants. The present invention also provides a novel Synechocystis mutant phytoene desaturase (PDS) gene conferring resistance to 4′-fluoro-6-[(alpha, alpha, alpha,-trfluoro-m-tolyl)oxy]-picolinamide, a bleaching herbicide. The present invention provides improved methods involving cyanobacteria for the screening of compounds, including a new high throughput protocol that is a rapid and cost effective way to identify target site genes.
摘要翻译：本发明提供用于植物转化和选择的ahas和pds核酸的替代来源。特别地，本发明提供了来自蓝细菌的Ahas和pds核酸，例如集胞藻，以及用于控制质体中表达的这些基因的表达元件。本发明还提供了编码乙酰乳酸合酶（ahas）大亚基的核酸和被发现提供对植物的除草剂抗性的小亚单位。本发明还提供了赋予对4'-氟-6 - [（α，α，α， - 氟 - 间甲苯基）氧基] - 甲酰胺酰胺（漂白除草剂）的抗性的新颖的集胞藻突变体植物衍生物去饱和酶（PDS）基因。本发明提供了涉及用于筛选化合物的蓝细菌的改进方法，包括新的高通量方案，其是识别靶位点基因的快速和成本有效的方法。

2. 发明授权

US07285701B2 Cyanobacterial nucleic acid fragments encoding proteins useful for controlling plant traits via nuclear or plastome transformation 有权
标题翻译：编码可用于通过核或plastome转化来控制植物性状的蛋白质的蓝细菌核酸片段
公开(公告)号：US07285701B2
公开(公告)日：2007-10-23
申请号：US11244732
申请日：2005-10-06
申请人： Genichi Kakefuda , Rui-Guang Zhen
发明人： Genichi Kakefuda , Rui-Guang Zhen
IPC分类号： A01H1/00 , C12N9/00 , C12N15/00
CPC分类号： C07K14/195 , C12Q1/689
摘要： This invention provides cyanobacteria as an alternative source of ahas and pds genes for plant transformations and for selectable markers. In particular, it provides for cyanobacteria, for example, Synechocystis, as a source of genes encoding herbicide insensitive proteins, and elements of genes for control of expression in plastids. Nucleic acid fragments, both the acetolactate synthase (ahas) large subunit and the ahas small subunit, were found to provide herbicide resistance. Also, the present invention provides novel Synechocystis mutant phytoene desaturase (PDS) gene conferring resistance to 4′-fluoro-6[(alpha,alpha,alpha,-trifluoro-m-tolyl)oxy]-picolinamide, a bleaching herbicide. The present invention provides improvements to method involving cyanobacteria for the screening of compounds, including a new high-through-put protocol that is a rapid and cost effective way to identify target site genes.
摘要翻译：本发明提供蓝藻作为植物转化和可选择标记的ahas和pds基因的替代来源。特别地，其提供蓝细菌，例如集胞藻，作为编码除草剂不敏感蛋白的基因的来源，以及用于控制质体中表达的基因的元件。发现核酸片段，乙酰乳酸合酶（ahas）大亚基和ahas小亚单位，提供除草剂抗性。此外，本发明提供了赋予对4'-氟-6 [（α，α，α， - 三氟 - 间甲苯基）氧基] - 甲酰胺酰胺（漂白除草剂）的抗性的新颖的集胞藻突变体八氢番茄红素去饱和酶（PDS）基因。本发明提供了涉及用于筛选化合物的蓝细菌的方法的改进，包括新的高通量方案，其是鉴定靶位点基因的快速和成本有效的方法。

3. 发明申请

US20060143730A1 Cyanobacterial nucleic acid fragments encoding proteins useful for controlling plant traits via nuclear or plastome transformation 有权
标题翻译：编码可用于通过核或plastome转化来控制植物性状的蛋白质的蓝细菌核酸片段
公开(公告)号：US20060143730A1
公开(公告)日：2006-06-29
申请号：US11244732
申请日：2005-10-06
申请人： Genichi Kakefuda , Hans-Ulrich Koop , Stephen Sturner , Rui-Guang Zhen
发明人： Genichi Kakefuda , Hans-Ulrich Koop , Stephen Sturner , Rui-Guang Zhen
IPC分类号： A01H1/00 , C07H21/04 , C12N15/82 , C12N5/04 , C12N9/80
CPC分类号： C07K14/195 , C12Q1/689
摘要： This invention provides cyanobacteria as an alternative source of ahas and pds genes for plant transformations and for selectable markers. In particular, it provides for cyanobacteria, for example, Synechocystis, as a source of genes encoding herbicide insensitive proteins, and elements of genes for control of expression in plastids. Nucleic acid fragments, both the acetolactate synthase (ahas) large subunit and the ahas small subunit, were found to provide herbicide resistance. Also, the present invention provides novel Synechocystis mutant phytoene desaturase (PDS) gene conferring resistance to 4′-fluoro-6[(alpha,alpha,alpha,-trifluoro-m-tolyl)oxy]-picolinamide, a bleaching herbicide. The present invention provides improvements to method involving cyanobacteria for the screening of compounds, including a new high-through-put protocol that is a rapid and cost effective way to identify target site genes.
摘要翻译：本发明提供蓝藻作为植物转化和可选择标记的ahas和pds基因的替代来源。特别地，其提供蓝细菌，例如集胞藻，作为编码除草剂不敏感蛋白的基因的来源，以及用于控制质体中表达的基因的元件。发现核酸片段，乙酰乳酸合酶（ahas）大亚基和ahas小亚单位，提供除草剂抗性。此外，本发明提供了赋予对4'-氟-6 [（α，α，α， - 三氟 - 间甲苯基）氧基] - 甲酰胺酰胺（漂白除草剂）的抗性的新颖的集胞藻突变体八氢番茄红素去饱和酶（PDS）基因。本发明提供了涉及用于筛选化合物的蓝细菌的方法的改进，包括新的高通量方案，其是鉴定靶位点基因的快速和成本有效的方法。

4. 发明授权

US08563806B2 Nematode inducible promotors and methods of use 失效
标题翻译：线虫诱导型启动子和使用方法
公开(公告)号：US08563806B2
公开(公告)日：2013-10-22
申请号：US12522260
申请日：2008-02-04
申请人： Aaron Wiig , Robert Ascenzi , Xiang Huang , Sumita Chaudhuri , Rui-Guang Zhen , Yu Han
发明人： Aaron Wiig , Robert Ascenzi , Xiang Huang , Sumita Chaudhuri , Rui-Guang Zhen , Yu Han
IPC分类号： C12N15/82 , C12N15/87 , C12N15/63
CPC分类号： C07K14/415 , C12N15/8227 , C12N15/8239
摘要： The invention provides isolated promoter polynucleotides that are root-specific and/or induced by plant parasitic nematodes. The promoters of the invention are useful for controlling expression of nucleic acids of interest in plant roots and are particularly useful for controlling transcription of nucleic acids encoding agents that disrupt formation or maintenance of parasitic nematode feeding sites in plants.
摘要翻译：本发明提供了由植物寄生线虫根特异性和/或诱导的分离的启动子多核苷酸。本发明的启动子可用于控制植物根中感兴趣的核酸的表达，并且特别可用于控制编码破坏植物中寄生线虫饲养位点的形成或维持的试剂的转录。

5. 发明申请

US20090271891A1 Pathogen Inducible Plant Thehalose-6-Phophate Phophatase Gene Promoters and Regulatory Elements 失效
标题翻译：病原体诱导植物海马-6-磷酸酶基因启动子和调节元件
公开(公告)号：US20090271891A1
公开(公告)日：2009-10-29
申请号：US12518676
申请日：2007-12-12
申请人： Aaron Wiig , Robert Ascenzi , Xiang Huang , Sumita Chaudhuri , Rui-Guang Zhen , Yu Han
发明人： Aaron Wiig , Robert Ascenzi , Xiang Huang , Sumita Chaudhuri , Rui-Guang Zhen , Yu Han
IPC分类号： A01H1/00 , C12N15/63 , C07H21/04 , A01H5/00
CPC分类号： C12N15/8239 , C12N9/16 , C12N15/8227
摘要： The invention provides plant gene promoters and regulatory elements that are root-specific and/or induced by parasitic nematodes. The promoters of the invention are useful for controlling expression of nucleic acids of interest in plants roots.
摘要翻译：本发明提供植物基因启动子和根系特异性和/或寄生线虫诱导的调控元件。本发明的启动子可用于控制植物根中感兴趣的核酸的表达。

6. 发明申请

US20090089896A1 PLANT METABOLITE EXPORTER GENE PROMOTERS 失效
标题翻译：植物代谢物出口基因促进剂
公开(公告)号：US20090089896A1
公开(公告)日：2009-04-02
申请号：US12280186
申请日：2007-02-13
申请人： Aaron Wiig , Robert Ascenzi , Sumita Chaudhuri , Xiang Huang , Rui-Guang Zhen , Yu Han
发明人： Aaron Wiig , Robert Ascenzi , Sumita Chaudhuri , Xiang Huang , Rui-Guang Zhen , Yu Han
IPC分类号： A01H1/00 , C07H21/00 , C12N15/63 , C12N5/04 , A01H5/00
CPC分类号： C12N15/8239 , C12N15/8227 , C12N15/8285 , Y02A40/164
摘要： The invention provides plant gene promoters, and essential promoter elements, which are root-specific and/or induced by parasitic nematodes. The promoters of the invention are useful for controlling expression of nucleic acids of interest in plant roots.
摘要翻译：本发明提供植物基因启动子和必需的启动子元件，其是根特异性的和/或由寄生线虫诱导的。本发明的启动子可用于控制植物根目的感兴趣的核酸的表达。

7. 发明授权

US08691962B2 Peroxidase gene nematode inducible promotors and methods of use 失效
标题翻译：过氧化物酶基因线虫诱导型启动子及其使用方法
公开(公告)号：US08691962B2
公开(公告)日：2014-04-08
申请号：US12519530
申请日：2007-12-20
申请人： Aaron Wiig , Robert Ascenzi , Xiang Huang , Sumita Chaudhuri , Rui-Guang Zhen , Yu Han
发明人： Aaron Wiig , Robert Ascenzi , Xiang Huang , Sumita Chaudhuri , Rui-Guang Zhen , Yu Han
IPC分类号： C12N15/63 , C12N15/82 , A01H5/00
CPC分类号： C12N15/8285 , C12N15/8239 , Y02A40/164
摘要： The invention provides isolated plant gene promoters and regulatory elements that are root specific and/or induced by plant parasitic nematodes. The promoters of the invention are useful for controlling expression of nucleic acids of interest in plant roots and are particularly useful for controlling transcription of nucleic acids encoding agents that disrupt formation or maintenance of parasitic nematode feeding sites in plants.
摘要翻译：本发明提供了植物寄生线虫根系特异性和/或诱导的分离的植物基因启动子和调控元件。本发明的启动子可用于控制植物根中感兴趣的核酸的表达，并且特别可用于控制编码破坏植物中寄生线虫饲养位点的形成或维持的试剂的转录。

8. 发明授权

US08053630B2 Nematode inducible plant metabolite exporter gene promoters 失效
标题翻译：线虫诱导型植物代谢产物出口基因启动子
公开(公告)号：US08053630B2
公开(公告)日：2011-11-08
申请号：US12280186
申请日：2007-02-13
申请人： Aaron Wig , Robert Ascenzi , Sumita Chaudhuri , Xiang Huang , Rui-Guang Zhen , Yu Han
发明人： Aaron Wig , Robert Ascenzi , Sumita Chaudhuri , Xiang Huang , Rui-Guang Zhen , Yu Han
IPC分类号： A01H5/00 , A01H1/00 , C07H21/04
CPC分类号： C12N15/8239 , C12N15/8227 , C12N15/8285 , Y02A40/164
摘要： The invention provides plant gene promoters, and essential promoter elements, which are root-specific and/or induced by parasitic nematodes. The promoters of the invention are useful for controlling expression of nucleic acids of interest in plant roots.
摘要翻译：本发明提供植物基因启动子和必需的启动子元件，其是根特异性的和/或由寄生线虫诱导的。本发明的启动子可用于控制植物根目的感兴趣的核酸的表达。

9. 发明申请

US20100077506A1 Peroxidase Gene Nematode Inducible Promotors and Methods of Use 失效
标题翻译：过氧化物酶基因线虫诱导启动子和使用方法
公开(公告)号：US20100077506A1
公开(公告)日：2010-03-25
申请号：US12519530
申请日：2007-12-20
申请人： Aaron Wiig , Robert Ascenzi , Xiang Huang , Sumita Chaudhuri , Rui-Guang Zhen , Yu Han
发明人： Aaron Wiig , Robert Ascenzi , Xiang Huang , Sumita Chaudhuri , Rui-Guang Zhen , Yu Han
IPC分类号： C12N15/82 , C07H21/04 , A01H5/00
CPC分类号： C12N15/8285 , C12N15/8239 , Y02A40/164
摘要： The invention provides plant gene promoters and regulatory elements that are root-specific and/or induced by parasitic nematodes. The promoters of the invention are useful for controlling expression of nucleic acids of interest in plants roots.
摘要翻译：本发明提供植物基因启动子和根系特异性和/或寄生线虫诱导的调控元件。本发明的启动子可用于控制植物根中感兴趣的核酸的表达。

10. 发明授权

US08546647B2 Pathogen inducible plant thehalose-6-phophate phophatase gene promoters and regulatory elements 失效
标题翻译：病原体诱导植物海洛因-6-磷酸酶基因启动子和调控元件
公开(公告)号：US08546647B2
公开(公告)日：2013-10-01
申请号：US12518676
申请日：2007-12-12
申请人： Aaron Wiig , Robert Ascenzi , Xiang Huang , Sumita Chaudhuri , Rui-Guang Zhen , Yu Han
发明人： Aaron Wiig , Robert Ascenzi , Xiang Huang , Sumita Chaudhuri , Rui-Guang Zhen , Yu Han
IPC分类号： C12N15/82 , C12N15/63 , C12N15/87
CPC分类号： C12N15/8239 , C12N9/16 , C12N15/8227
摘要： The invention provides plant gene promoters and regulatory elements that are root-specific and/or induced by parasitic nematodes. The promoters of the invention are useful for controlling expression of nucleic acids of interest in plants' roots. The invention also provides expression cassettes including the plant gene promoters and regulatory elements of the invention, transgenic plants containing such expression cassettes, and methods of producing such transgenic plants.
摘要翻译：本发明提供植物基因启动子和根系特异性和/或寄生线虫诱导的调控元件。本发明的启动子可用于控制植物根中感兴趣的核酸的表达。本发明还提供包括本发明的植物基因启动子和调控元件，含有这种表达盒的转基因植物以及生产这种转基因植物的方法的表达盒。

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