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1. 发明申请

US20110008770A1 COMPOSITIONS AND METHODS FOR REVERSE TRANSCRIPTION OF NUCLEIC ACID MOLECULES 审中-公开
标题翻译：反向转录核酸分子的组合物和方法
公开(公告)号：US20110008770A1
公开(公告)日：2011-01-13
申请号：US12511569
申请日：2009-07-29
申请人： Gary F. Gerard , Michael D. Smith , Deb K. Chatterjee
发明人： Gary F. Gerard , Michael D. Smith , Deb K. Chatterjee
IPC分类号： C12Q1/68 , C12N9/12 , C12P19/34
CPC分类号： C12N15/1096 , C12N9/1276 , C12N2740/10011 , C12N2740/11011 , C12Q1/6848 , C12Q2527/101 , C12Q2521/301 , C12Q2521/107
摘要： The present invention is generally related to compositions and methods for the reverse transcription of nucleic acid molecules, especially messenger RNA molecules. Specifically, the invention relates to compositions comprising mixtures of polypeptides having reverse transcriptase (RT) activity, and to methods of producing, amplifying or sequencing nucleic acid molecules (particularly cDNA molecules) using these compositions or polypeptides, particularly at temperatures above about 55° C. The invention also relates to nucleic acid molecules produced by these methods, to vectors and host cells comprising these nucleic acid molecules, and to the use of such nucleic acid molecules to produce desired polypeptides. The invention also relates to methods for producing Rous Sarcoma Virus (RSV) and Avian Myeloblastosis Virus (AMV) RTs or other Avian Sarcoma-Leukosis Virus (ASLV) RTs (α and/or β subunits thereof), to isolated nucleic acid molecules encoding such RSV RT, AMV RT or other ASLV RT subunits, to vectors and host cells comprising these isolated nucleic acid molecules and to RSV RT, AMV RT and other ASLV RT subunits produced by these methods. The invention further relates to nucleic acid molecules encoding recombinant heterodimeric RT holoenzymes, particularly heterodimeric RSV RTs, AMV RTs or other ASLV RTs (which may be αβ RTs, ββ RTs, or α RTs), vectors (particularly baculovirus vectors) and host cells (particularly insect and yeast cells) comprising these nucleic acid molecules, methods for producing these heterodimeric RTs and heterodimeric RTs produced by these methods. The invention also relates to kits comprising the compositions, polypeptides, or RSV RTs, AMV RTs or other ASLV RTs of the invention.
摘要翻译：本发明通常涉及核酸分子，特别是信使RNA分子的逆转录的组合物和方法。具体地，本发明涉及包含具有逆转录酶（RT）活性的多肽的混合物的组合物，以及使用这些组合物或多肽，特别是在高于约55℃的温度下产生，扩增或测序核酸分子（特别是cDNA分子）的方法本发明还涉及通过这些方法产生的核酸分子，涉及包含这些核酸分子的载体和宿主细胞，以及使用这种核酸分子产生所需多肽。本发明还涉及用于生产分离的核酸分子编码的Rous肉瘤病毒（RSV）和禽成髓细胞增殖症病毒（AMV）RT或其他禽类肉瘤 - 白血病病毒（ASLV）RT（α和/或其亚基）的方法这样的RSV RT，AMV RT或其他ASLV RT亚基，涉及包含这些分离的核酸分子的载体和宿主细胞以及通过这些方法产生的RSV RT，AMV RT和其他ASLV RT亚基。本发明还涉及编码重组异源二聚体RT全酶，特别是异二聚体RSV RT，AMV RT或其它ASLV RT（其可以是α和bgr; RTs，bgr; RT或RT）的载体（特别是杆状病毒载体）的核酸分子，和包含这些核酸分子的宿主细胞（特别是昆虫和酵母细胞），通过这些方法生产这些异源二聚体RT和异二聚体RT的方法。本发明还涉及包含本发明的组合物，多肽或RSV RT，AMV RT或其他ASLV RT的试剂盒。

2. 发明授权

US06989259B2 Recombinant methods for making reverse transcriptases and mutants thereof 失效
标题翻译：制备逆转录酶及其突变体的重组方法
公开(公告)号：US06989259B2
公开(公告)日：2006-01-24
申请号：US09064057
申请日：1998-04-22
申请人： Gary F. Gerard , Michael D. Smith , Deb K. Chatterjee
发明人： Gary F. Gerard , Michael D. Smith , Deb K. Chatterjee
IPC分类号： C12N9/12
CPC分类号： C12N15/1096 , C12N9/1276 , C12N2740/10011 , C12N2740/11011 , C12Q1/6848 , C12Q2527/101 , C12Q2521/301 , C12Q2521/107
摘要： The invention relates to compositions comprising mixtures of polypeptides having reverse transcriptase (RT) activity and to methods of producing, amplifying or sequencing nucleic acid molecules using these compositions or polypeptides, particularly at temperatures above about 55° C. The invention also relates to nucleic acid molecules produced by these methods, to vectors and host cells comprising these nucleic acid molecules, and use of such nucleic acid molecules to produce desired polypeptide. The invention also relates to methods for producing Avian Sarcoma-Leukosis Virus (ASLV) RT subunits, in particular, Avian Myeloblastosis Virus (AMV) RTs, to isolated nucleic acid molecules encoding ASLV RT subunits, and to ASLV RT subunits produced by these methods. The invention further relates to nucleic acid molecules encoding recombinant RT holoenzymes, particularly ASLV RTs, methods for producing these RTs and to RTs produced by these methods. The invention also relates to kits comprising the compositions, polypeptides, and ASLV RTs of the invention.
摘要翻译：本发明涉及包含具有逆转录酶（RT）活性的多肽的混合物的组合物以及使用这些组合物或多肽，特别是在高于约55℃的温度下产生，扩增或测序核酸分子的方法。本发明还涉及核酸通过这些方法产生的分子与包含这些核酸分子的载体和宿主细胞，以及使用这种核酸分子产生所需多肽。本发明还涉及用于将编码ASLV RT亚基的分离的核酸分子以及通过这些方法产生的ASLV RT亚基生产禽类肉瘤 - 白血病病毒（ASLV）RT亚单位，特别是禽成髓细胞病毒（AMV）RT）的方法。本发明还涉及编码重组RT全酶，特别是ASLV RT的核酸分子，用于制备这些RT的方法和通过这些方法产生的RT。本发明还涉及包含本发明的组合物，多肽和ASLV RT的试剂盒。

3. 发明授权

US06835561B1 Composition of reverse transcriptases and mutants thereof 有权
标题翻译：逆转录酶及其突变体的组成
公开(公告)号：US06835561B1
公开(公告)日：2004-12-28
申请号：US09245025
申请日：1999-02-05
申请人： Gary F. Gerard , Michael D. Smith , Deb K. Chatterjee
发明人： Gary F. Gerard , Michael D. Smith , Deb K. Chatterjee
IPC分类号： C12N912
CPC分类号： C12N15/1096 , C12N9/1276 , C12N2740/10011 , C12N2740/11011 , C12Q1/6848 , C12Q2527/101 , C12Q2521/301 , C12Q2521/107
摘要： The invention relates to compositions comprising mixtures of polypeptides having reverse transcriptase (RT) activity and to methods of producing, amplifying or sequencing nucleic acid molecules using these compositions or polypeptides, particularly at temperatures above about 55° C. The invention also relates to nucleic acid molecules produced by these methods, to vectors and host cells comprising these nucleic acid molecules, and use of such nucleic acid molecules to produce desired polypeptide. The invention also relates to methods for producing Avian Sarcoma-Leukosis Virus (ASLV) RT subunits, in particular, Avian Myeloblastosis Virus (AMV) RTs, to isolated nucleic acid molecules encoding ASLV RT subunits, and to ASLV RT subunits produced by these methods. The invention further relates to nucleic acid molecules encoding recombinant RT holoenzymes, particularly ASLV RTs, methods for producing these RTs and to RTs produced by these methods. The invention also relates to kits comprising the compositions, polypeptides, and ASLV RTs of the invention.
摘要翻译：本发明涉及包含具有逆转录酶（RT）活性的多肽的混合物的组合物以及使用这些组合物或多肽，特别是在高于约55℃的温度下产生，扩增或测序核酸分子的方法。本发明还涉及核酸通过这些方法产生的分子与包含这些核酸分子的载体和宿主细胞，以及使用这种核酸分子产生所需多肽。本发明还涉及用于将编码ASLV RT亚基的分离的核酸分子以及通过这些方法产生的ASLV RT亚基生产禽类肉瘤 - 白血病病毒（ASLV）RT亚单位，特别是禽成髓细胞病毒（AMV）RT）的方法。本发明还涉及编码重组RT全酶，特别是ASLV RT的核酸分子，用于制备这些RT的方法和通过这些方法产生的RT。本发明还涉及包含本发明的组合物，多肽和ASLV RT的试剂盒。

4. 发明授权

US06518019B2 Compositions and methods for reverse transcription of nucleic acid molecules 有权
标题翻译：核酸分子逆转录的组成和方法
公开(公告)号：US06518019B2
公开(公告)日：2003-02-11
申请号：US09245026
申请日：1999-02-05
申请人： Gary F. Gerard , Michael D. Smith , Deb K. Chatterjee
发明人： Gary F. Gerard , Michael D. Smith , Deb K. Chatterjee
IPC分类号： C12Q168
CPC分类号： C12N15/1096 , C12N9/1276 , C12N2740/10011 , C12N2740/11011 , C12Q1/6848 , C12Q2527/101 , C12Q2521/301 , C12Q2521/107
摘要： The present invention is generally related to compositions and methods for the reverse transcription of nucleic acid molecules, especially messenger RNA molecules. Specifically, the invention relates to compositions comprising mixtures of polypeptides having reverse transcriptase (RT) activity, and to methods of producing, amplifying or sequencing nucleic acid molecules (particularly cDNA molecules) using these compositions or polypeptides, particularly at temperatures about about 55° C. The invention also relates to nucleic acid molecules produced by these methods, to vectors and host cells comprising these nucleic acid molecules, and to the use of such nucleic acid molecules to produce desired polypeptides. The invention also relates to methods for producing Rous Sarcoma Virus (RSV) and Avian Myeloblastosis Virus (AMV) RTs or other Avian Sarcoma-Leukosis Virus (ASLV) RTs (&agr; and/or &bgr; subunits thereof), to isolated nucleic acid molecules encoding such RSV RT, AMV RT or other ASLV RT subunits, to vectors and host cells comprising these isolated nucleic acid molecules and to RSV RT, AMV RT and other ASLV RT subunits produced by these methods. The invention further relates to nucleic acid molecules encoding recombinant heterodimeric RT holoenzymes, particularly heterodimeric RSV RTs, AMV RTs or other ASLV RTs (which may be &agr;&bgr; RTs, &bgr;&bgr; RTs, or &agr; RTs), vectors (particularly baculovirus vectors) and host cells (particularly insect and yeast cells) comprising these nucleic acid molecules, methods for producing these heterodimeric RTs and heterodimeric RTs produced by these methods. The invention also relates to kits comprising the compositions, polypeptides, or RSV RTs, AMV RTs or other ASLV RTs of the invention.
摘要翻译：本发明通常涉及核酸分子，特别是信使RNA分子的逆转录的组合物和方法。具体地，本发明涉及包含具有逆转录酶（RT）活性的多肽的混合物的组合物，以及使用这些组合物或多肽，特别是在约55℃的温度下产生，扩增或测序核酸分子（特别是cDNA分子）的方法本发明还涉及通过这些方法产生的核酸分子，涉及包含这些核酸分子的载体和宿主细胞，以及使用这种核酸分子产生所需多肽。本发明还涉及用于生产分离的编码这样的核酸分子的Rous肉瘤病毒（RSV）和禽成髓细胞病毒（AMV）RT或其他禽类肉瘤 - 白血病病毒（ASLV）RT（其α和/或β亚基））的方法 RSV RT，AMV RT或其他ASLV RT亚单位，包含这些分离的核酸分子的载体和宿主细胞以及通过这些方法产生的RSV RT，AMV RT和其他ASLV RT亚基。本发明还涉及编码重组异源二聚体RT全酶，特别是异二聚体RSV RT，AMV RT或其他ASLV RT（其可以是字母表RT，betabeta RT或αRT），载体（特别是杆状病毒载体）和宿主细胞（特别是昆虫和酵母细胞），通过这些方法产生的这些异源二聚体RT和异二聚体RT的制备方法。本发明还涉及包含本发明的组合物，多肽或RSV RT，AMV RT或其他ASLV RT的试剂盒。

5. 发明申请

US20110160289A1 COMPOSITIONS AND METHODS FOR ENHANCED SENSITIVITY AND SPECIFICITY OF NUCLEIC ACID SYNTHESIS 审中-公开
标题翻译：增强敏感性和核酸合成特异性的组合物和方法
公开(公告)号：US20110160289A1
公开(公告)日：2011-06-30
申请号：US12772961
申请日：2010-05-03
申请人： Mekbib ASTATKE , Deb K. Chatterjee , Gary F. Gerard
发明人： Mekbib ASTATKE , Deb K. Chatterjee , Gary F. Gerard
IPC分类号： A61K31/7052 , C07H21/00 , C12N9/12 , C12P19/34 , C12Q1/68 , C12N9/99 , A61P31/12
CPC分类号： C12N15/11 , A61K31/7052 , C12N9/1247 , C12N9/1252 , C12N9/1276 , C12N2310/122 , C12N2310/315 , C12P19/34 , C12Q1/6844 , C12Q1/6869 , C12Y207/07006 , C12Y207/07007 , C12Y207/07049 , C12Q2527/125
摘要： The present invention relates to nucleic acid inhibitors, compositions and method for enhancing synthesis of nucleic acid molecules. In a preferred aspect, the invention relates to inhibition or control of nucleic acid synthesis, sequencing or amplification. Specifically, the present invention discloses nucleic acids having affinity for polypeptides with polymerase activity for use in such synthesis, amplification or sequencing reactions. The nucleic acid inhibitors are capable of inhibiting nonspecific nucleic acid synthesis under certain conditions (e.g., at ambient temperatures). Thus, in a preferred aspect, the invention relates to “hot start” synthesis of nucleic acid molecules. Accordingly, the invention prevents, reduces or substantially reduces nonspecific nucleic acid synthesis. The invention also relates to kits for synthesizing, amplifying, reverse transcribing or sequencing nucleic acid molecules comprising one or more of the nucleic acid inhibitors or compositions of the invention. The invention also relates to using the inhibitors of the invention to prevent viral replication or treat viral infections in a subject. Thus, the invention relates to therapeutic methods and pharmaceutical compositions using the inhibitors of the invention. The invention thus may be used for in vivo and in vitro inhibition of nucleic acid synthesis and/or inhibition of polymerase activity.
摘要翻译：本发明涉及用于增强核酸分子合成的核酸抑制剂，组合物和方法。在优选的方面，本发明涉及抑制或控制核酸合成，测序或扩增。具体而言，本发明公开了对于在这种合成，扩增或测序反应中使用具有聚合酶活性的多肽具有亲和力的核酸。核酸抑制剂能够在某些条件下（例如在环境温度下）抑制非特异性核酸合成。因此，在优选的方面，本发明涉及核酸分子的“热启动”合成。因此，本发明防止，减少或显着降低非特异性核酸合成。本发明还涉及用于合成，扩增，逆转录或测序核酸分子的试剂盒，所述核酸分子包含一种或多种本发明的核酸抑制剂或组合物。本发明还涉及使用本发明的抑制剂来预防病毒复制或治疗受试者的病毒感染。因此，本发明涉及使用本发明的抑制剂的治疗方法和药物组合物。因此，本发明可用于体内和体外抑制核酸合成和/或抑制聚合酶活性。

6. 发明申请

US20080171388A1 COMPOSITIONS AND METHODS FOR ENHANCED SENSITIVITY AND SPECIFICITY OF NUCLEIC ACID SYNTHESIS 审中-公开
标题翻译：增强敏感性和核酸合成特异性的组合物和方法
公开(公告)号：US20080171388A1
公开(公告)日：2008-07-17
申请号：US11852156
申请日：2007-09-07
申请人： Mekbib Astatke , Deb K. Chatterjee , Gary F. Gerard
发明人： Mekbib Astatke , Deb K. Chatterjee , Gary F. Gerard
IPC分类号： C12N5/06
CPC分类号： C12N15/11 , A61K31/7052 , C12N9/1247 , C12N9/1252 , C12N9/1276 , C12N2310/122 , C12N2310/315 , C12P19/34 , C12Q1/6844 , C12Q1/6869 , C12Y207/07006 , C12Y207/07007 , C12Y207/07049 , C12Q2527/125
摘要： The present invention relates to nucleic acid inhibitors, compositions and method for enhancing synthesis of nucleic acid molecules. In a preferred aspect, the invention relates to inhibition or control of nucleic acid synthesis, sequencing or amplification. Specifically, the present invention discloses nucleic acids having affinity for polypeptides with polymerase activity for use in such synthesis, amplification or sequencing reactions. The nucleic acid inhibitors are capable of inhibiting nonspecific nucleic acid synthesis under certain conditions (e.g., at ambient temperatures). Thus, in a preferred aspect, the invention relates to “hot start” synthesis of nucleic acid molecules. Accordingly, the invention prevents, reduces or substantially reduces nonspecific nucleic acid synthesis. The invention also relates to kits for synthesizing, amplifying, reverse transcribing or sequencing nucleic acid molecules comprising one or more of the nucleic acid inhibitors or compositions of the invention. The invention also relates to using the inhibitors of the invention to prevent viral replication or treat viral infections in a subject. Thus, the invention relates to therapeutic methods and pharmaceutical compositions using the inhibitors of the invention. The invention thus may be used for in vivo and in vitro inhibition of nucleic acid synthesis and/or inhibition of polymerase activity.
摘要翻译：本发明涉及用于增强核酸分子合成的核酸抑制剂，组合物和方法。在优选的方面，本发明涉及抑制或控制核酸合成，测序或扩增。具体而言，本发明公开了对于在这种合成，扩增或测序反应中使用具有聚合酶活性的多肽具有亲和力的核酸。核酸抑制剂能够在某些条件下（例如在环境温度下）抑制非特异性核酸合成。因此，在优选的方面，本发明涉及核酸分子的“热启动”合成。因此，本发明防止，减少或显着降低非特异性核酸合成。本发明还涉及用于合成，扩增，逆转录或测序核酸分子的试剂盒，所述核酸分子包含一种或多种本发明的核酸抑制剂或组合物。本发明还涉及使用本发明的抑制剂来预防病毒复制或治疗受试者的病毒感染。因此，本发明涉及使用本发明的抑制剂的治疗方法和药物组合物。因此，本发明可用于体内和体外抑制核酸合成和/或抑制聚合酶活性。

7. 发明授权

US06830902B1 Compositions and methods for enhanced sensitivity and specificity of nucleic acid synthesis 有权
标题翻译：增强核酸合成灵敏度和特异性的组合物和方法
公开(公告)号：US06830902B1
公开(公告)日：2004-12-14
申请号：US09608066
申请日：2000-06-30
申请人： Mekbib Astatke , Deb K. Chatterjee , Gary F. Gerard
发明人： Mekbib Astatke , Deb K. Chatterjee , Gary F. Gerard
IPC分类号： C12P1934
CPC分类号： C12N15/11 , A61K31/7052 , C12N9/1247 , C12N9/1252 , C12N9/1276 , C12N2310/122 , C12N2310/315 , C12P19/34 , C12Q1/6844 , C12Q1/6869 , C12Y207/07006 , C12Y207/07007 , C12Y207/07049 , C12Q2527/125
摘要： The present invention relates to nucleic acid inhibitors, compositions and method for enhancing synthesis of nucleic acid molecules. In a preferred aspect, the invention relates to inhibition or control of nucleic acid synthesis, sequencing or amplification. Specifically, the present invention discloses nucleic acids having affinity for polypeptides with polymerase activity for use in such synthesis, amplification or sequencing reactions. The nucleic acid inhibitors are capable of inhibiting nonspecific nucleic acid synthesis under certain conditions (e.g., at ambient temperatures). Thus, in a preferred aspect, the invention relates to “hot start” synthesis of nucleic acid molecules. Accordingly, the invention prevents, reduces or substantially reduces nonspecific nucleic acid synthesis. The invention also relates to kits for synthesizing, amplifying, reverse transcribing or sequencing nucleic acid molecules comprising one or more of the nucleic acid inhibitors or compositions of the invention. The invention also relates to using the inhibitors of the invention to prevent viral replication or treat viral infections in a subject. Thus, the invention relates to therapeutic methods and pharmaceutical compositions using the inhibitors of the invention. The invention thus may be used for in vivo and in vitro inhibition of nucleic acid synthesis and/or inhibition of polymerase activity.
摘要翻译：本发明涉及用于增强核酸分子合成的核酸抑制剂，组合物和方法。在优选的方面，本发明涉及抑制或控制核酸合成，测序或扩增。具体而言，本发明公开了对于在这种合成，扩增或测序反应中使用具有聚合酶活性的多肽具有亲和力的核酸。核酸抑制剂能够在某些条件下（例如在环境温度下）抑制非特异性核酸合成。因此，在优选的方面，本发明涉及核酸分子的“热启动”合成。因此，本发明防止，减少或显着降低非特异性核酸合成。本发明还涉及用于合成，扩增，逆转录或测序核酸分子的试剂盒，所述核酸分子包含一种或多种本发明的核酸抑制剂或组合物。本发明还涉及使用本发明的抑制剂来预防病毒复制或治疗受试者的病毒感染。因此，本发明涉及使用本发明的抑制剂的治疗方法和药物组合物。因此，本发明可用于体内和体外抑制核酸合成和/或抑制聚合酶活性。

8. 发明授权

US08043816B2 Compositions and methods for temperature-dependent nucleic acid synthesis 有权
标题翻译：用于温度依赖性核酸合成的组合物和方法
公开(公告)号：US08043816B2
公开(公告)日：2011-10-25
申请号：US12468036
申请日：2009-05-18
申请人： Mekbib Astatke , Deb K. Chatterjee , Gary F. Gerard
发明人： Mekbib Astatke , Deb K. Chatterjee , Gary F. Gerard
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12N15/11 , A61K31/7052 , C12N9/1247 , C12N9/1252 , C12N9/1276 , C12N2310/122 , C12N2310/315 , C12P19/34 , C12Q1/6844 , C12Q1/6869 , C12Y207/07006 , C12Y207/07007 , C12Y207/07049 , C12Q2527/125
摘要： The present invention relates to nucleic acid inhibitors, compositions and method for enhancing synthesis of nucleic acid molecules. In a preferred aspect, the invention relates to inhibition or control of nucleic acid synthesis, sequencing or amplification. Specifically, the present invention discloses nucleic acids having affinity for polypeptides with polymerase activity for use in such synthesis, amplification or sequencing reactions. The nucleic acid inhibitors are capable of inhibiting nonspecific nucleic acid synthesis under certain conditions (e.g., at ambient temperatures). Thus, in a preferred aspect, the invention relates to “hot start” synthesis of nucleic acid molecules. Accordingly, the invention prevents, reduces or substantially reduces nonspecific nucleic acid synthesis. The invention also relates to kits for synthesizing, amplifying, reverse transcribing or sequencing nucleic acid molecules comprising one or more of the nucleic acid inhibitors or compositions of the invention. The invention also relates to using the inhibitors of the invention to prevent viral replication or treat viral infections in a subject. Thus, the invention relates to therapeutic methods and pharmaceutical compositions using the inhibitors of the invention. The invention thus may be used for in vivo and in vitro inhibition of nucleic acid synthesis and/or inhibition of polymerase activity.
摘要翻译：本发明涉及用于增强核酸分子合成的核酸抑制剂，组合物和方法。在优选的方面，本发明涉及抑制或控制核酸合成，测序或扩增。具体而言，本发明公开了对于在这种合成，扩增或测序反应中使用具有聚合酶活性的多肽具有亲和力的核酸。核酸抑制剂能够在某些条件下（例如在环境温度下）抑制非特异性核酸合成。因此，在优选的方面，本发明涉及核酸分子的“热启动”合成。因此，本发明防止，减少或显着降低非特异性核酸合成。本发明还涉及用于合成，扩增，逆转录或测序核酸分子的试剂盒，所述核酸分子包含一种或多种本发明的核酸抑制剂或组合物。本发明还涉及使用本发明的抑制剂来预防病毒复制或治疗受试者的病毒感染。因此，本发明涉及使用本发明的抑制剂的治疗方法和药物组合物。因此，本发明可用于体内和体外抑制核酸合成和/或抑制聚合酶活性。

9. 发明申请

US20090275100A1 COMPOSITIONS AND METHODS FOR ENHANCED SENSITIVITY AND SPECIFICITY OF NUCLEIC ACID SYNTHESIS 有权
标题翻译：增强敏感性和核酸合成特异性的组合物和方法
公开(公告)号：US20090275100A1
公开(公告)日：2009-11-05
申请号：US12468036
申请日：2009-05-18
申请人： Mekbib Astatke , Deb K. Chatterjee , Gary F. Gerard
发明人： Mekbib Astatke , Deb K. Chatterjee , Gary F. Gerard
IPC分类号： C12N9/99
CPC分类号： C12N15/11 , A61K31/7052 , C12N9/1247 , C12N9/1252 , C12N9/1276 , C12N2310/122 , C12N2310/315 , C12P19/34 , C12Q1/6844 , C12Q1/6869 , C12Y207/07006 , C12Y207/07007 , C12Y207/07049 , C12Q2527/125
摘要： The present invention relates to nucleic acid inhibitors, compositions and method for enhancing synthesis of nucleic acid molecules. In a preferred aspect, the invention relates to inhibition or control of nucleic acid synthesis, sequencing or amplification. Specifically, the present invention discloses nucleic acids having affinity for polypeptides with polymerase activity for use in such synthesis, amplification or sequencing reactions. The nucleic acid inhibitors are capable of inhibiting nonspecific nucleic acid synthesis under certain conditions (e.g., at ambient temperatures). Thus, in a preferred aspect, the invention relates to “hot start” synthesis of nucleic acid molecules. Accordingly, the invention prevents, reduces or substantially reduces nonspecific nucleic acid synthesis. The invention also relates to kits for synthesizing, amplifying, reverse transcribing or sequencing nucleic acid molecules comprising one or more of the nucleic acid inhibitors or compositions of the invention. The invention also relates to using the inhibitors of the invention to prevent viral replication or treat viral infections in a subject. Thus, the invention relates to therapeutic methods and pharmaceutical compositions using the inhibitors of the invention. The invention thus may be used for in vivo and in vitro inhibition of nucleic acid synthesis and/or inhibition of polymerase activity.
摘要翻译：本发明涉及用于增强核酸分子合成的核酸抑制剂，组合物和方法。在优选的方面，本发明涉及抑制或控制核酸合成，测序或扩增。具体而言，本发明公开了对于在这种合成，扩增或测序反应中使用具有聚合酶活性的多肽具有亲和力的核酸。核酸抑制剂能够在某些条件下（例如在环境温度下）抑制非特异性核酸合成。因此，在优选的方面，本发明涉及核酸分子的“热启动”合成。因此，本发明防止，减少或显着降低非特异性核酸合成。本发明还涉及用于合成，扩增，逆转录或测序核酸分子的试剂盒，所述核酸分子包含一种或多种本发明的核酸抑制剂或组合物。本发明还涉及使用本发明的抑制剂来预防病毒复制或治疗受试者的病毒感染。因此，本发明涉及使用本发明的抑制剂的治疗方法和药物组合物。因此，本发明可用于体内和体外抑制核酸合成和/或抑制聚合酶活性。

10. 发明申请

US20110081704A1 THERMOSTABLE REVERSE TRANSCRIPTASES AND USES THEREOF 有权
标题翻译：可逆转的转录物及其用途
公开(公告)号：US20110081704A1
公开(公告)日：2011-04-07
申请号：US12861797
申请日：2010-08-23
申请人： Michael D. Smith , Robert Jason Potter , Gulshan Dhariwal , Gary F. Gerard , Kim Rosenthal
发明人： Michael D. Smith , Robert Jason Potter , Gulshan Dhariwal , Gary F. Gerard , Kim Rosenthal
IPC分类号： C12N9/12
CPC分类号： C12N9/1276
摘要： The present invention is in the fields of molecular and cellular biology. The invention is generally related to reverse transcriptase enzymes and methods for the reverse transcription of nucleic acid molecules, especially messenger RNA molecules. Specifically, the invention relates to reverse transcriptase enzymes which have been mutated or modified to increase thermostability, decrease terminal deoxynucleotidyl transferase activity, and/or increase fidelity, and to methods of producing, amplifying or sequencing nucleic acid molecules (particularly cDNA molecules) using these reverse transcriptase enzymes or compositions. The invention also relates to nucleic acid molecules produced by these methods and to the use of such nucleic acid molecules to produce desired polypeptides. The invention also concerns kits comprising such enzymes or compositions.
摘要翻译：本发明涉及分子和细胞生物学领域。本发明通常涉及逆转录酶和逆转录核酸分子，特别是信使RNA分子的方法。具体地，本发明涉及已被突变或修饰以增加热稳定性，降低末端脱氧核苷酸转移酶活性和/或增加保真度的逆转录酶，以及使用这些方法来产生，扩增或测序核酸分子（特别是cDNA分子）的方法逆转录酶或组合物。本发明还涉及通过这些方法产生的核酸分子以及这种核酸分子用于产生所需多肽的用途。本发明还涉及包含这些酶或组合物的试剂盒。

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