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    • 4. 发明申请
    • NOVEL METHODS
    • 新方法
    • WO2008079943A3
    • 2008-11-06
    • PCT/US2007088310
    • 2007-12-20
    • SMITHKLINE BEECHAM CORPTAYLOR ALEXANDER HKRISHNA DELFI
    • TAYLOR ALEXANDER HKRISHNA DELFI
    • C12N15/09C12N15/00C12N15/63
    • C12N15/1082C12N15/1086
    • The present invention provides methods for site-specifically integrating at least one first nucleic acid into a genome of at least one cell, comprising: transforming said genome with a reporter nucleic acid construct that is linked to a first att site; selecting at least one first transformed cell having at least one integrated reporter nucleic acid construct; introducing said at least one first nucleic acid and a homologous recombination mediating enzyme into said cell wherein said at least one first nucleic acid comprises at least one second att site that is complementary to said first att site; and maintaining said cell under conditions sufficient for said at least one first nucleic acid to integrate into said first att site producing at least one stably intergrated cell.
    • 本发明提供了用于将至少一种第一核酸位点特异性整合到至少一种细胞的基因组中的方法,包括:用与第一att位点连接的报道核酸构建体转化所述基因组; 选择具有至少一个整合的报道子核酸构建体的至少一个第一转化细胞; 将所述至少一种第一核酸和同源重组介导酶导入所述细胞中,其中所述至少一种第一核酸包含与所述第一att位点互补的至少一个第二att位点; 并且将所述细胞保持在足以使所述至少一种第一核酸整合到所述第一att位点的条件下,从而产生至少一个稳定整合的细胞。