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1. 发明申请

WO9846772A3 GENE CONVERSION AS A TOOL FOR THE CONSTRUCTION OF RECOMBINANT INDUSTRIAL FILAMENTOUS FUNGI 审中-公开
标题翻译：基因转化作为重组工业真菌真菌的工具
公开(公告)号：WO9846772A3
公开(公告)日：1999-02-25
申请号：PCT/EP9802070
申请日：1998-04-09
申请人： GIST BROCADES BV , SELTEN GERARDUS CORNELIS MARIA , SWINKELS BART WILLEM , BOVENBERG ROELOF ARY LANS
发明人： SELTEN GERARDUS CORNELIS MARIA , SWINKELS BART WILLEM , BOVENBERG ROELOF ARY LANS
IPC分类号： C12N15/09 , C12N1/15 , C12N9/00 , C12N9/16 , C12N15/52 , C12N15/62 , C12N15/65 , C12N15/80 , C12P21/00
CPC分类号： C12N9/16 , C12N9/00 , C12N15/62 , C12N15/80
摘要： The present invention relates to filamentous fungi that comprise in their genomes at last two substantially homologous DNA domains which are suitable for integration of one or more copies of a recombinant DNA molecule and wherein at least two of these DNA domains comprise an integrated copy of a recombinant DNA molecule. The invention also relates to methods for preparing such filamentous fungi and for further multiplying the DNA domains with integrated recombinant DNA molecules through gene conversion or amplification.

2. 发明申请

WO9706261A3 THE USE OF HOMOLOGOUS AMDS GENES AS SELECTABLE MARKERS 审中-公开
标题翻译：使用同种异体AMDS基因作为选择性标记
公开(公告)号：WO9706261A3
公开(公告)日：1997-03-13
申请号：PCT/EP9603494
申请日：1996-08-05
申请人： GIST BROCADES BV , SWINKELS BART WILLEM , SELTEN GERARDUS CORNELIS MARIA , BAKHUIS JANNA GARDINA , BOVENBERG ROELOF ARY LANS , VOLLEBREGT ADRIANUS WILHELMUS
发明人： SWINKELS BART WILLEM , SELTEN GERARDUS CORNELIS MARIA , BAKHUIS JANNA GARDINA , BOVENBERG ROELOF ARY LANS , VOLLEBREGT ADRIANUS WILHELMUS
IPC分类号： C12N15/09 , C12N1/15 , C12N9/80 , C12N15/00 , C12R1/685 , C12N15/52 , C12N1/19 , C12N15/90
CPC分类号： C12N9/80
摘要： The present invention discloses novel amdS genes from fungi previously not known to contain and amdS gene, such as Aspergillus niger and Penicillium chrysogenum. The novel amdS genes can be used as homologous selectable marker genes in the transformation of these fungi. Alternatively, the cloned amdS genes can be used to inactivate the endogenous copy of the gene in order to reduce the background in transformation experiments.

3. 发明申请

WO9802567A3 NOVEL PROCESS FOR THE PREPARATION OF CEPHALOSPORINS USING ACREMONIUM CHRYSOGENUM 审中-公开
标题翻译：使用枸杞子制备碳水化合物的新方法
公开(公告)号：WO9802567A3
公开(公告)日：1998-02-19
申请号：PCT/EP9703878
申请日：1997-07-15
申请人： GIST BROCADES BV , BOVENBERG ROELOF ARY LANS , SCHIPPER DIRK , KERKMAN RICHARD
发明人： BOVENBERG ROELOF ARY LANS , SCHIPPER DIRK , KERKMAN RICHARD
IPC分类号： C12N15/09 , C12N9/10 , C12P35/00 , C12P35/04 , C12R1/75
CPC分类号： C12P35/04 , C12N9/1029
摘要： The present invention describes a process wherein Acremonium is used as a suitable and convenient organism for the production of N-acylated cephalosporin derivatives. In the process of the invention, a recombinant, acyltransferase-expressing Acremonium strain is cultured in the presence of a suitable acyl side chain precursor. Optionally, the acyltransferase-expressing Acremonium strain further does not express hydroxylase and/or acetyltransferase activity. The N-acylated cephalosporin derivative is recovered without contaminating α-aminoadipyl cephalosporin compounds. The thus recovered compound subsequently may be deacylated via chemical or enzymatical means.

4. 发明申请

WO9802551A2 IMPROVED PROCESS FOR THE PRODUCTION OF ADIPOYL CEPHALOSPORINS 审中-公开
标题翻译：用于生产丙烯酰胆碱的改进方法
公开(公告)号：WO9802551A2
公开(公告)日：1998-01-22
申请号：PCT/EP9703879
申请日：1997-07-15
申请人： GIST BROCADES BV , BOVENBERG ROELOF ARY LANS , LAAN JAN METSKE V D , KERKMAN RICHARD , NIEBOER MAARTEN
发明人： BOVENBERG ROELOF ARY LANS , VAN DER LAAN JAN METSKE , KERKMAN RICHARD , NIEBOER MAARTEN
IPC分类号： C12N9/02 , C12P35/02 , C12N15/52 , C12N1/15 , C12N9/00 , C12P35/00
CPC分类号： C12N9/0071 , C12P35/02
摘要： An improved process for the preparation of adipoyl cephalosporins via enzymatic ring expansion of adipoyl-6-aminopenicillinic acid, using a Penicillium chrysogenum transformant strain expressing modified expandase enzyme.
摘要翻译：通过己二酰-6-氨基青霉烯酸的酶环扩展制备己二酰头孢菌素的改进方法，使用表达修饰的扩环酶的产黄青霉转化株。

5. 发明申请

WO9720053A3 IMPROVED PROCESS FOR THE PRODUCTION OF SEMI-SYNTHETIC CEPHALOSPORINS VIA EXPANDASE ACTIVITY ON PENICILLIN G 审中-公开
标题翻译：通过PENICILLIN G的扩增活性生产半合成CEPHALOSPORIN的改进方法
公开(公告)号：WO9720053A3
公开(公告)日：1997-07-17
申请号：PCT/EP9605358
申请日：1996-11-27
申请人： GIST BROCADES BV , UNIV OXFORD , SUTHERLAND JOHN DAVID , BOVENBERG ROELOF ARY LANS , LAAN JAN METSKE V D
发明人： SUTHERLAND JOHN DAVID , BOVENBERG ROELOF ARY LANS , VAN DER LAAN JAN METSKE
IPC分类号： C12N1/21 , C12N9/00 , C12N9/02 , C12N15/52 , C12P35/00
CPC分类号： C12N9/0071 , C12P35/00
摘要： An overall process for the preparation and recovery of 7-aminodesacetoxycephalosporanic acid (7-ADCA) via enzymatic ring expansion activity on penicillin G, using a modified expandase enzyme.

6. 发明申请

WO0071579A3 REGULATING ORGANELLE HOMEOSTASIS IN A CELL FACTORY 审中-公开
标题翻译：调节细胞工厂的有机肥料
公开(公告)号：WO0071579A3
公开(公告)日：2001-03-01
申请号：PCT/EP0004718
申请日：2000-05-22
申请人： DSM NV , BOVENBERG ROELOF ARY LANS , DEN BERG MARCO ALEXANDER VAN , NIEBOER MAARTEN , VEENHUIS MARTEN , KIEL JAN ANDRIES KORNELIS WILL , KLEI IDA JOHANNA V D
发明人： BOVENBERG ROELOF ARY LANS , VAN DEN BERG MARCO ALEXANDER , NIEBOER MAARTEN , VEENHUIS MARTEN , KIEL JAN ANDRIES KORNELIS WILL , VAN DER KLEI IDA JOHANNA
IPC分类号： C07K14/37 , C07K14/385 , C12N1/19 , C12N15/31 , C12N15/80 , C12P37/00 , C12R1/82
CPC分类号： C12R1/78 , C07K14/37 , C07K14/385 , C12N15/80 , C12P37/00 , C12R1/82
摘要： The present invention relates to a method for modulating the production of secondary metabolites in the fungus Penicillium chrysogenum by influencing the homeostasis of organelles by modulating a least one gene which is directly related to organelle homeostasis in this fungus. Preferably, the organelle homeostasis is modulated by genetically engineering at least one gene encoding a protein which is responsible for organelle biogenesis (the expression and/or activity of which is to be increased) and/or at least one gene encoding a protein which is responsible for organelle degeneration (the expression and/or activity of which is to be decreased). Genes involved in organelle biosynthesis in P. chrysogenum are exemplified by the so-called pex11 gene. Genes involved in organelle degradation are exemplified by the so-called vps15 and pdd1 genes.
摘要翻译：本发明涉及通过调节与该真菌中的细胞器稳态直接相关的至少一种基因来影响细胞器的稳态来调节产生真菌青霉菌的次生代谢物的方法。优选地，通过遗传工程来编码至少一个编码负责细胞器生物发生的蛋白质（其表达和/或活性将被增加）的基因和/或至少一个编码负责蛋白质的基因的基因调节细胞器体内平衡用于细胞器变性（其表达和/或活性将被降低）。参与细胞器生物合成的基因。产黄素例如所谓的pex11基因。涉及细胞器降解的基因通过所谓的vps15和pdd1基因来说明。

7. 发明申请

WO0132904A3 METHOD FOR ENHANCING SECRETION OF BETA-LACTAM COMPOUNDS 审中-公开
标题翻译：用于增强BETA-LACTAM化合物分泌的方法
公开(公告)号：WO0132904A3
公开(公告)日：2001-11-08
申请号：PCT/EP0011489
申请日：2000-11-03
申请人： DSM NV , BERG VAN DEN MARCO ALEXANDER , BOVENBERG ROELOF ARY LANS , DRIESSEN ARNOLD JACOB MATTIEU , KONINGS WILHELMUS NICOLAAS , SCHUURS THEO AUKE , NIEBOER MAARTEN , WESTERLAKEN ILJA
发明人： BERG VAN DEN MARCO ALEXANDER , BOVENBERG ROELOF ARY LANS , DRIESSEN ARNOLD JACOB MATTIEU , KONINGS WILHELMUS NICOLAAS , SCHUURS THEO AUKE , NIEBOER MAARTEN , WESTERLAKEN ILJA
IPC分类号： C07K14/385 , C12N9/12 , C12N9/14 , C12N15/31 , C12P17/18 , C12P35/00 , C12P37/00
CPC分类号： C07K14/385 , C12P37/00
摘要： An method for enhancing the secretion of a beta -lactam compound from a micro-organism is described, together with an isolated polynucleotide encoding the ABC transporter protein responsible for said enhanced secretion. Furthermore an isolated poylnucleotide encoding an ABC transporter is claimed. Method for producing beta -lactam compounds in a micro-organism comprising the step of enhancing ABC-transporter activity of said micro-organism and subsequently culturing said micro-organism under conditions allowing the production of said beta -lactam compounds is also disclosed.
摘要翻译：与来自微生物的β-内酰胺化合物分泌增强的方法一起描述了编码负责所述增强分泌的ABC转运蛋白的分离的多核苷酸。此外，要求保护编码ABC转运蛋白的孤立的寡核苷酸。还公开了在微生物中产生β-内酰胺化合物的方法，其包括增强所述微生物的ABC-转运蛋白活性并随后在允许产生所述β-内酰胺化合物的条件下培养所述微生物的步骤。

8. 发明申请

WO0037671A3 IMPROVED IN VIVO PRODUCTION OF CEPHALOSPORINS 审中-公开
标题翻译：改善头孢菌素的体内生产
公开(公告)号：WO0037671A3
公开(公告)日：2000-09-14
申请号：PCT/EP9910292
申请日：1999-12-21
申请人： DSM NV , BOVENBERG ROELOF ARY LANS , KERKMAN RICHARD , KOENHEN ERIC
发明人： BOVENBERG ROELOF ARY LANS , KERKMAN RICHARD , KOENHEN ERIC
IPC分类号： C12P35/06 , C12N9/10 , C12P35/00 , C12R1/82
CPC分类号： C12P35/00 , C12N9/1029
摘要： The present invention discloses a process for the production of 7-amino cephalosporanic acid (7-ACA) or a derivative thereof comprising the steps of fermenting a P. chrysogenum strain being transformed with an expression construct comprising a nucleotide sequence encoding expandase, hydroxylase and acetyltransferase activity in the presence of a suitable acyl side chain precursor, or a salt or ester thereof, such that an N-acylated 7-ACA compound is produced, N-deacylating the thus produced N-acylated 7-ACA compound and, optionally, acylating the free amino group and/or substituting the 3' acetate group with a side chain suitable to form a cephalosporin antibiotic, characterised in that the nucleotide sequence encoding the acetyltransferase is derived from Acremonium chrysogenum and starts at the second ATG of the open reading frame as present in said nucleotide sequence.
摘要翻译：本发明公开了生产7-氨基头孢菌酸（7-ACA）或其衍生物的方法，其包括发酵产黄青霉菌菌株的步骤，所述产黄青霉菌菌株用包含编码扩环酶，羟化酶和乙酰转移酶的核苷酸序列的表达构建体转化在合适的酰基侧链前体或其盐或酯存在下的活性，从而产生N-酰化的7-ACA化合物，将由此产生的N-酰化的7-ACA化合物N-脱酰基化，和任选地酰化游离氨基和/或用适于形成头孢菌素抗生素的侧链取代3'乙酸酯基团，其特征在于编码乙酰转移酶的核苷酸序列来自Acremonium chrysogenum并开始于开放阅读框的第二个ATG，存在于所述核苷酸序列中。

9. 发明申请

WO9960102A3 IMPROVED IN VIVO PRODUCTION OF CEPHALOSPORINS 审中-公开
标题翻译：改进生物素生物素
公开(公告)号：WO9960102A3
公开(公告)日：2000-02-03
申请号：PCT/EP9903455
申请日：1999-05-19
申请人： DSM NV , NIEBOER MAARTEN , BOVENBERG ROELOF ARY LANS
发明人： NIEBOER MAARTEN , BOVENBERG ROELOF ARY LANS
IPC分类号： C12N15/09 , C12N1/15 , C12N1/21 , C12N9/00 , C12N9/02 , C12N15/52 , C12N15/53 , C12P35/00 , C12P35/06 , C12R1/80 , C12R1/82
CPC分类号： C12N9/0071 , C12P35/00
摘要： The invention provides DNA, heterologous host cells capable of transcribing, translating or expressing said DNA and methods employing such host cells and cultures thereof for an improved in vivo production of acylated cephalosporins with higher yield. According to the invention, a host cell is provided comprising an enzyme having expandase activity which is predominantly localised in the cytosol (as opposed to localised mainly in or with the peroxisomes or microbodies) of the host cell.
摘要翻译：本发明提供DNA，能够转录，翻译或表达所述DNA的异源宿主细胞，以及使用这种宿主细胞及其培养物的方法，以更高的产率改进体内产生的酰化头孢菌素。根据本发明，提供宿主细胞，其包含主要位于宿主细胞中的主要位于胞质溶胶（与主要在或与过氧化物酶体或微体中定位的相反）的具有扩展酶活性的酶。

10. 发明申请

WO2010015627A3 ADIPOYL-7-ADCA PRODUCING STRAINS 审中-公开
标题翻译： ADIPOYL-7-ADCA生产菌株
公开(公告)号：WO2010015627A3
公开(公告)日：2010-09-30
申请号：PCT/EP2009060090
申请日：2009-08-04
申请人： DSM IP ASSETS BV , VAN DEN BERG MARCO ALEXANDER , BOVENBERG ROELOF ARY LANS
发明人： VAN DEN BERG MARCO ALEXANDER , BOVENBERG ROELOF ARY LANS
IPC分类号： C12N9/00 , C12N9/10
CPC分类号： C12P37/00 , C12N9/13 , C12N9/93
摘要： The present invention relates to a mutant microbial strain derived from a parent microbial strain capable of producing an N-adipoylated ß-lactam compound when cultured in a medium comprising adipic acid characterized in that the mutant microbial strain has an improved incorporation of the adipic acid from the culture medium into the N-adipoylated ß-lactam compound.
摘要翻译：本发明涉及一种衍生自亲本微生物菌株的突变微生物菌株，当在包含己二酸的培养基中培养时能够产生N-己二酰化的β-内酰胺化合物，其特征在于所述突变微生物菌株具有改进的来自己二酸的来自将培养基转化为N-己二酰化的β-内酰胺化合物。

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