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1. 发明申请

WO2009086423A2 YEAST ORGANISM PRODUCING ISOBUTANOL AT A HIGH YIELD 审中-公开
标题翻译： YEAST ORGANISM在高产量下生产ISOBUTANOL
公开(公告)号：WO2009086423A2
公开(公告)日：2009-07-09
申请号：PCT/US2008/088235
申请日：2008-12-23
申请人： GEVO, INC. , FELDMAN, Reid, M. Renny , GUNAWARDENA, Uvini , URANO, Jun , MEINHOLD, Peter , ARISTIDOU, Aristos , DUNDON, Catherine, Asleson , SMITH, Christopher
发明人： FELDMAN, Reid, M. Renny , GUNAWARDENA, Uvini , URANO, Jun , MEINHOLD, Peter , ARISTIDOU, Aristos , DUNDON, Catherine, Asleson , SMITH, Christopher
IPC分类号： C12P7/00
CPC分类号： C12N9/88 , C12P7/16 , C12Y401/01001 , Y02E50/10
摘要： There is disclosed a method of producing isobutanol. Sn an embodiment, the method includes providing a microorganism transformed with an isobutanoi producing pathway containing at least one exogenous gene. The microorganism is selected to produce isobutanol from a carbon source at a yield of at least 10 percent theoretical. The method includes cultivating the microorganism in a culture medium containing a feedstock providing the carbon source, until isobutanol is produced. The method includes recovering the isobutanol. In one embodiment, the microorganism is a yeast with a Crabtree-negative phenotype. In another embodiment, the microorganism is a yeast microorganism with a Crabtree-positive phenotype. There is disclosed a microorganism for producing isobutanol. In an embodiment, the microorganism includes an isobutanol producing pathway containing at least one exogenous gene, and is selected to produce a recoverable quantity of isobutanol from a carbon source at a yield of at least 10 percent theoretical.
摘要翻译：公开了异丁醇的制造方法。在一个实施方案中，该方法包括提供转化了含有至少一种外源基因的异丁酸生产途径的微生物。选择微生物以产生至少10％的理论值从碳源产生异丁醇。该方法包括在含有提供碳源的原料的培养基中培养微生物，直到产生异丁醇。该方法包括回收异丁醇。在一个实施方案中，微生物是具有Crabtree-阴性表型的酵母。在另一个实施方案中，微生物是具有Crabtree阳性表型的酵母微生物。公开了用于生产异丁醇的微生物。在一个实施方案中，微生物包括含有至少一种外源基因的异丁醇生产途径，并且选择其以至少10％理论值的产率从碳源产生可回收量的异丁醇。

2. 发明申请

WO2010151525A1 YEAST ORGANISMS FOR THE PRODUCTION OF ISOBUTANOL 审中-公开
标题翻译：用于生产ISOBUTANOL的YEAST有机体
公开(公告)号：WO2010151525A1
公开(公告)日：2010-12-29
申请号：PCT/US2010/039447
申请日：2010-06-22
申请人： GEVO, INC. , FELDMAN, Reid M. Renny , GUNAWARDENA, Uvini , URANO, Jun , MEINHOLD, Peter , ARISTIDOU, Aristos , DUNDON, Catherine Asleson , SMITH, Christopher
发明人： FELDMAN, Reid M. Renny , GUNAWARDENA, Uvini , URANO, Jun , MEINHOLD, Peter , ARISTIDOU, Aristos , DUNDON, Catherine Asleson , SMITH, Christopher
IPC分类号： C12P7/16
CPC分类号： C12P7/16 , C12N1/16 , Y02E50/10
摘要： The present invention provides recombinant microorganisms comprising an isobutanol producing metabolic pathway and methods of using said recombinant microorganisms to produce isobutanol. In various aspects of the invention, the recombinant microorganisms may comprise a modification resulting in the reduction of pyruvate decarboxylase and/or glycerol-3-phosphate dehydrogenase activity. In various embodiments described herein, the recombinant microorganisms may be microorganisms of the Saccharomyces clade, Crabtree-negative yeast microorganisms, Crabtree-positive yeast microorganisms, post-WGD (whole genome duplication) yeast microorganisms, pre-WGD (whole genome duplication) yeast microorganisms, and non-fermenting yeast microorganisms.
摘要翻译：本发明提供包含产生异丁醇的代谢途径的重组微生物和使用所述重组微生物产生异丁醇的方法。在本发明的各个方面，重组微生物可以包含导致丙酮酸脱羧酶和/或甘油-3-磷酸脱氢酶活性降低的修饰。在本文所述的各种实施方案中，重组微生物可以是酵母进化枝，Crabtree阴性酵母微生物，Crabtree阳性酵母微生物，WGD（全基因组复制）酵母微生物，WGD前（全基因组复制）酵母微生物，和非发酵酵母微生物。

3. 发明申请

WO2008080124A2 BUTANOL PRODUCTION BY METABOLICALLY ENGINEERED YEAST 审中-公开
标题翻译：用代谢工程酵母生产丁醇
公开(公告)号：WO2008080124A2
公开(公告)日：2008-07-03
申请号：PCT/US2007/088705
申请日：2007-12-21
申请人： GEVO , GUNAWARDENA, Uvini , MEINHOLD, Peter , PETERS, Matthew W. , URANO, Jun , FELDMAN, Reid M. Renny
发明人： GUNAWARDENA, Uvini , MEINHOLD, Peter , PETERS, Matthew W. , URANO, Jun , FELDMAN, Reid M. Renny
IPC分类号： C12P7/16
CPC分类号： C12P7/16 , C12N9/0008 , C12N9/88 , C12N9/93 , Y02E50/10
摘要： There are disclosed metabolically-engineered yeast and methods of producing n-butanol. In an embodiment, metabolically-engineered yeast is capable of metabolizing a carbon source to produce n-butanol, at least one pathway produces increased cytosolic acetyl-CoA relative to cytosolic acetyl-CoA produced by a wild-type yeast, and at least one heterologous gene encodes and expresses at least one enzyme for a metabolic pathway capable of utilizing NADH to convert acetyl-CoA to n-butanol. In another embodiment, a method of producing n-butanol includes (a) providing metabolically-engineered yeast capable of metabolizing a carbon source to produce n-butanol, at least one pathway produces increased cytosolic acetyl-CoA relative to cytosolic acetyl-CoA produced by a wild-type yeast, and at least one heterologous gene encodes and expresses at least one enzyme for a metabolic pathway utilizing NADH to convert acetyl-CoA to n-butanol; and (b) culturing the yeast to produce n-butanol. Other embodiments are also disclosed.
摘要翻译：公开了代谢工程酵母和生产正丁醇的方法。在一个实施方案中，代谢工程化的酵母能够代谢碳源以产生正丁醇，相对于由野生型酵母产生的胞质乙酰-CoA，至少一种途径产生增加的胞质乙酰-CoA，并且至少一种异源基因编码和表达至少一种能够利用NADH将乙酰-CoA转化为正丁醇的代谢途径的酶。在另一个实施方案中，生产正丁醇的方法包括（a）提供能够代谢碳源以产生正丁醇的代谢工程化的酵母，至少一个途径产生相对于由胞质乙酰-CoA产生的胞质乙酰-CoA增加的胞质乙酰-CoA 野生型酵母，并且至少一种异源基因编码并表达至少一种用于利用NADH将乙酰-CoA转化为正丁醇的代谢途径的酶; 和（b）培养酵母以产生正丁醇。其他实施例也被公开。

4. 发明公开

EP2121949A2 BUTANOL PRODUCTION BY METABOLICALLY ENGINEERED YEAST 有权
标题翻译：用代谢工程酵母生产丁醇
公开(公告)号：EP2121949A2
公开(公告)日：2009-11-25
申请号：EP07871733.7
申请日：2007-12-21
申请人： GEVO, Inc.
发明人： GUNAWARDENA, Uvini , MEINHOLD, Peter , PETERS, Matthew W. , URANO, Jun , FELDMAN, Reid M. Renny
IPC分类号： C12P7/16
CPC分类号： C12P7/16 , C12N9/0008 , C12N9/88 , C12N9/93 , Y02E50/10
摘要： There are disclosed metabolically-engineered yeast and methods of producing n-butanol. In an embodiment, metabolically-engineered yeast is capable of metabolizing a carbon source to produce n-butanol, at least one pathway produces increased cytosolic acetyl-CoA relative to cytosolic acetyl-CoA produced by a wild-type yeast, and at least one heterologous gene encodes and expresses at least one enzyme for a metabolic pathway capable of utilizing NADH to convert acetyl-CoA to n-butanol. In another embodiment, a method of producing n-butanol includes (a) providing metabolically-engineered yeast capable of metabolizing a carbon source to produce n-butanol, at least one pathway produces increased cytosolic acetyl-CoA relative to cytosolic acetyl-CoA produced by a wild-type yeast, and at least one heterologous gene encodes and expresses at least one enzyme for a metabolic pathway utilizing NADH to convert acetyl-CoA to n-butanol; and (b) culturing the yeast to produce n-butanol. Other embodiments are also disclosed.
摘要翻译：公开了代谢工程酵母和生产正丁醇的方法。在一个实施方案中，代谢工程化的酵母能够代谢碳源以产生正丁醇，相对于由野生型酵母产生的胞质乙酰-CoA，至少一种途径产生增加的胞质乙酰-CoA，并且至少一种异源基因编码和表达至少一种能够利用NADH将乙酰-CoA转化为正丁醇的代谢途径的酶。在另一个实施方案中，生产正丁醇的方法包括（a）提供能够代谢碳源以产生正丁醇的代谢工程化的酵母，至少一个途径产生相对于由胞质乙酰-CoA产生的胞质乙酰-CoA增加的胞质乙酰-CoA 野生型酵母，并且至少一种异源基因编码并表达至少一种用于利用NADH将乙酰-CoA转化为正丁醇的代谢途径的酶; 和（b）培养酵母以产生正丁醇。其他实施例也被公开。

5. 发明授权

EP2121949B1 BUTANOL PRODUCTION BY METABOLICALLY ENGINEERED YEAST 有权
标题翻译：用代谢工程酵母生产丁醇
公开(公告)号：EP2121949B1
公开(公告)日：2013-05-08
申请号：EP07871733.7
申请日：2007-12-21
申请人： GEVO, Inc.
发明人： GUNAWARDENA, Uvini , MEINHOLD, Peter , PETERS, Matthew W. , URANO, Jun , FELDMAN, Reid M. Renny
IPC分类号： C12N9/88 , C12N9/00 , C12N9/02 , C12P7/16
CPC分类号： C12P7/16 , C12N9/0008 , C12N9/88 , C12N9/93 , Y02E50/10

6. 发明公开

EP2235193A2 YEAST ORGANISM PRODUCING ISOBUTANOL AT A HIGH YIELD 有权
标题翻译：酵母生物收入丰富的生产异丁醇
公开(公告)号：EP2235193A2
公开(公告)日：2010-10-06
申请号：EP08867473.4
申请日：2008-12-23
申请人： GEVO, Inc.
发明人： FELDMAN, Reid, M. Renny , GUNAWARDENA, Uvini , URANO, Jun , MEINHOLD, Peter , ARISTIDOU, Aristos , DUNDON, Catherine, Asleson , SMITH, Christopher
IPC分类号： C12P7/16 , C12N15/52
CPC分类号： C12N9/88 , C12P7/16 , C12Y401/01001 , Y02E50/10
摘要： There is disclosed a method of producing isobutanol. Sn an embodiment, the method includes providing a microorganism transformed with an isobutanoi producing pathway containing at least one exogenous gene. The microorganism is selected to produce isobutanol from a carbon source at a yield of at least 10 percent theoretical. The method includes cultivating the microorganism in a culture medium containing a feedstock providing the carbon source, until isobutanol is produced. The method includes recovering the isobutanol. In one embodiment, the microorganism is a yeast with a Crabtree-negative phenotype. In another embodiment, the microorganism is a yeast microorganism with a Crabtree-positive phenotype. There is disclosed a microorganism for producing isobutanol. In an embodiment, the microorganism includes an isobutanol producing pathway containing at least one exogenous gene, and is selected to produce a recoverable quantity of isobutanol from a carbon source at a yield of at least 10 percent theoretical.

7. 发明授权

EP2235193B1 YEAST ORGANISM PRODUCING ISOBUTANOL AT A HIGH YIELD 有权
标题翻译：酵母生物收入丰富的生产异丁醇
公开(公告)号：EP2235193B1
公开(公告)日：2015-11-25
申请号：EP08867473.4
申请日：2008-12-23
申请人： GEVO, Inc.
发明人： FELDMAN, Reid, M. Renny , GUNAWARDENA, Uvini , URANO, Jun , MEINHOLD, Peter , ARISTIDOU, Aristos , DUNDON, Catherine, Asleson , SMITH, Christopher
IPC分类号： C12N9/88 , C12P7/16
CPC分类号： C12N9/88 , C12P7/16 , C12Y401/01001 , Y02E50/10

8. 发明公开

EP2446043A1 YEAST ORGANISMS FOR THE PRODUCTION OF ISOBUTANOL 审中-公开
标题翻译：酵母生物用于生产异丁醇
公开(公告)号：EP2446043A1
公开(公告)日：2012-05-02
申请号：EP10792562.0
申请日：2010-06-22
申请人： GEVO, Inc.
发明人： FELDMAN, Reid M. Renny , GUNAWARDENA, Uvini , URANO, Jun , MEINHOLD, Peter , ARISTIDOU, Aristos , DUNDON, Catherine Asleson , SMITH, Christopher
IPC分类号： C12P7/16
CPC分类号： C12P7/16 , C12N1/16 , Y02E50/10
摘要： The present invention provides recombinant microorganisms comprising an isobutanol producing metabolic pathway and methods of using said recombinant microorganisms to produce isobutanol. In various aspects of the invention, the recombinant microorganisms may comprise a modification resulting in the reduction of pyruvate decarboxylase and/or glycerol-3-phosphate dehydrogenase activity. In various embodiments described herein, the recombinant microorganisms may be microorganisms of the Saccharomyces clade, Crabtree-negative yeast microorganisms, Crabtree-positive yeast microorganisms, post-WGD (whole genome duplication) yeast microorganisms, pre-WGD (whole genome duplication) yeast microorganisms, and non-fermenting yeast microorganisms.

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