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1. 发明申请

WO2013096322A8 ION EXCHANGE MEMBRANE CHROMATOGRAPHY 审中-公开
标题翻译：离子交换膜色谱
公开(公告)号：WO2013096322A8
公开(公告)日：2014-02-27
申请号：PCT/US2012070373
申请日：2012-12-18
申请人： GENENTECH INC , HOFFMANN LA ROCHE
发明人： BILL JEROME JOSEPH JR , BROWN ARICK MICHAEL , DOWD CHRISTOPHER JOHN , THAYER BROOKE ELLEN
IPC分类号： C07K1/18
CPC分类号： C07K1/18 , C07K16/065
摘要： Methods of enhancing efficiency of downstream chromatography steps for purification of proteins comprising: (a) passing a composition comprising a polypeptide of interest and various contaminants through an ion exchange membrane, wherein the polypeptide and the membrane have opposite charge, at operating conditions comprised of a buffer having a pH sufficiently distinct from the pI of the polypeptide to enhance the charge of the polypeptide and a low ionic strength effective to prevent the shielding of charges by buffer ions, which cause the membrane to bind the polypeptide and at least one contaminant, (b) overloading the ion exchange membrane such that at least one contaminant remains bound to the membrane while the polypeptide of interest is primarily in the effluent; (c) collecting the effluent from the ion exchange membrane comprising the polypeptide of interest; (d) subjecting the membrane effluent comprising the polypeptide of interest to a purification step of similar charge as the previous membrane, and (e) recovering the purified polypeptide from the effluent of the charged ion exchange chromatography purification step.
摘要翻译：提高用于纯化蛋白质的下游色谱步骤的效率的方法，包括：（a）使包含感兴趣的多肽和各种污染物的组合物通过离子交换膜，其中所述多肽和膜具有相反的电荷，在包含缓冲液具有与多肽的pI足够不同的pH，以增强多肽的电荷和低离子强度，有效地防止由缓冲离子屏蔽电荷，引起膜结合多肽和至少一种污染物（（ b）使离子交换膜过载，使得至少一种污染物保持与膜结合，而感兴趣的多肽主要在流出物中; （c）从包含感兴趣的多肽的离子交换膜收集流出物; （d）使包含所述多肽的膜流出物与之前的膜具有相似电荷的纯化步骤，和（e）从带电离子交换色谱纯化步骤的流出物中回收纯化的多肽。

2. 发明申请

WO2013096322A9 ION EXCHANGE MEMBRANE CHROMATOGRAPHY 审中-公开
标题翻译：离子交换膜层析
公开(公告)号：WO2013096322A9
公开(公告)日：2013-08-22
申请号：PCT/US2012070373
申请日：2012-12-18
申请人： GENENTECH INC , HOFFMANN LA ROCHE
发明人： BILL JEROME JOSEPH , BROWN ARICK MICHAEL , DOWD CHRISTOPHER JOHN , THAYER BROOKE ELLEN
IPC分类号： C07K1/18
CPC分类号： C07K1/18 , C07K16/065
摘要： Methods of enhancing efficiency of downstream chromatography steps for purification of proteins comprising: (a) passing a composition comprising a polypeptide of interest and various contaminants through an ion exchange membrane, wherein the polypeptide and the membrane have opposite charge, at operating conditions comprised of a buffer having a pH sufficiently distinct from the pi of the polypeptide to enhance the charge of the polypeptide and a low ionic strength effective to prevent the shielding of charges by buffer ions, which cause the membrane to bind the polypeptide and at least one contaminant, (b) overloading the ion exchange membrane such that at least one contaminant remains bound to the membrane while the polypeptide of interest is primarily in the effluent; (c) collecting the effluent from the ion exchange membrane comprising the polypeptide of interest; (d) subjecting the membrane effluent comprising the polypeptide of interest to a purification step of similar charge as the previous membrane, and (e) recovering the purified polypeptide from the effluent of the charged ion exchange chromatography purification step.
摘要翻译：提高蛋白质纯化的下游层析步骤的效率的方法，包括：（a）在包含目的多肽和各种污染物的操作条件下，使包含目的多肽和各种污染物的组合物通过离子交换膜，其中多肽和膜具有相反的电荷; 具有与多肽的pI充分不同的pH以增强多肽的电荷的pH和有效防止缓冲离子对电荷的屏蔽的低离子强度，缓冲离子引起膜结合多肽和至少一种污染物，（ b）过载离子交换膜，使得至少一种污染物保持与膜结合，而感兴趣的多肽主要在流出物中; （c）收集来自包含感兴趣多肽的离子交换膜的流出物; （d）使包含感兴趣多肽的膜流出物经历与先前膜相似电荷的纯化步骤，和（e）从带电离子交换色谱纯化步骤的流出物中回收纯化的多肽。

3. 发明申请

WO2013078170A8 PURIFICATION OF ANTI-C-MET ANTIBODIES 审中-公开
标题翻译：抗C-MET抗体的纯化
公开(公告)号：WO2013078170A8
公开(公告)日：2013-08-22
申请号：PCT/US2012066004
申请日：2012-11-20
申请人： GENENTECH INC , HOFFMANN LA ROCHE
发明人： PERSSON JOSEFINE , WONG MARC , BILL JEROME JOSEPH JR , BROWN ARICK MICHAEL , GIESE GLEN SCOTT , HSII JUDY FAY-CHEN , LIM AMY , RADHAMOHAN ASHA NANDINI , RODRIGUEZ MARICEL
IPC分类号： C07K16/00 , C07K16/28
CPC分类号： C07K16/2863 , A61K39/39558 , A61K45/06 , C07K1/14 , C07K1/18 , C07K1/22 , C07K1/34 , C07K1/36 , C07K16/065 , C07K16/32 , C07K2317/24 , C07K2317/56
摘要： Provided herein are methods of purifying anti-c-met antibodies, compositions and pharmaceutical formulations comprising purified anti-c-met antibodies, and methods of using the same.
摘要翻译：本文提供了纯化包含纯化的抗c-met抗体的抗c-met抗体，组合物和药物制剂的方法，以及使用其的方法。

4. 发明专利

HRP20181846T1 KROMATOGRAFIJA MEMBRANOM ZA IONSKU IZMJENU 未知
公开(公告)号：HRP20181846T1
公开(公告)日：2018-12-28
申请号：HRP20181846
申请日：2018-11-05
申请人： GENENTECH INC , HOFFMANN LA ROCHE
发明人： BILL JEROME JOSEPH , BROWN ARICK MICHAEL , DOWD CHRISTOPHER JOHN , THAYER BROOKE ELLEN
IPC分类号： C07K1/16

5. 发明专利

PL2794635T3 MEMBRANOWA CHROMATOGRAFIA JONOWYMIENNA 未知
公开(公告)号：PL2794635T3
公开(公告)日：2019-02-28
申请号：PL12806322
申请日：2012-12-18
申请人： HOFFMANN LA ROCHE
发明人： BILL JEROME JOSEPH JR , BROWN ARICK MICHAEL , DOWD CHRISTOPHER JOHN , THAYER BROOKE ELLEN
IPC分类号： C07K1/16

6. 发明专利

ES2697676T3 Cromatografía de membrana de intercambio iónico 未知
公开(公告)号：ES2697676T3
公开(公告)日：2019-01-25
申请号：ES12806322
申请日：2012-12-18
申请人： HOFFMANN LA ROCHE
发明人： BILL JEROME JOSEPH JR , BROWN ARICK MICHAEL , DOWD CHRISTOPHER JOHN , THAYER BROOKE ELLEN
IPC分类号： C07K1/16
摘要： Un procedimiento para potenciar la eficacia de etapas de cromatografía posteriores para la purificación de anticuerpos que comprende: a. pasar una composición que comprende un anticuerpo de interés y diversos contaminantes a través de una membrana de intercambio iónico, en el que el anticuerpo y la membrana tienen carga opuesta, en condiciones de funcionamiento que comprenden un tampón que tiene un pH suficientemente distinto del pI del anticuerpo para potenciar la carga del anticuerpo y una baja fuerza iónica eficaz para evitar el apantallamiento de las cargas por los iones del tampón, lo que provoca que la membrana se una al anticuerpo y a al menos un contaminante; b. sobrecargar la membrana de intercambio iónico a una densidad de carga de 1000-5000 g/l de modo que al menos un contaminante permanezca unido a la membrana mientras que el anticuerpo de interés se encuentra principalmente en el efluente; c. recoger el efluente de la membrana de intercambio iónico que comprende el anticuerpo de interés; d. someter el efluente de la membrana que comprende el anticuerpo de interés a una etapa de cromatografía de intercambio iónico de carga similar a la membrana anterior, y e. recuperar el anticuerpo purificado del efluente de la etapa de cromatografía de intercambio iónico cargada.

7. 发明专利

SI2794635T1 Ionskoizmenjevalna membranska kromatografija 未知
公开(公告)号：SI2794635T1
公开(公告)日：2018-12-31
申请号：SI201231443
申请日：2012-12-18
申请人： HOFFMANN LA ROCHE
发明人： BILL JEROME JOSEPH JR , BROWN ARICK MICHAEL , DOWD CHRISTOPHER JOHN , THAYER BROOKE ELLEN
IPC分类号： C07K1/00

8. 发明专利

HK1203526A1 ION EXCHANGE MEMBRANE CHROMATOGRAPHY 未知
公开(公告)号：HK1203526A1
公开(公告)日：2015-10-30
申请号：HK15104046
申请日：2015-04-27
申请人： HOFFMANN LA ROCHE
发明人： BILL JEROME JOSEPH JR , BROWN ARICK MICHAEL , DOWD CHRISTOPHER JOHN , THAYER BROOKE ELLEN
IPC分类号： C07K20060101
摘要： Methods of enhancing efficiency of downstream chromatography steps for purification of proteins comprising: (a) passing a composition comprising a polypeptide of interest and various contaminants through an ion exchange membrane, wherein the polypeptide and the membrane have opposite charge, at operating conditions comprised of a buffer having a pH sufficiently distinct from the pI of the polypeptide to enhance the charge of the polypeptide and a low ionic strength effective to prevent the shielding of charges by buffer ions, which cause the membrane to bind the polypeptide and at least one contaminant, (b) overloading the ion exchange membrane such that at least one contaminant remains bound to the membrane while the polypeptide of interest is primarily in the effluent; (c) collecting the effluent from the ion exchange membrane comprising the polypeptide of interest; (d) subjecting the membrane effluent comprising the polypeptide of interest to a purification step of similar charge as the previous membrane, and (e) recovering the purified polypeptide from the effluent of the charged ion exchange chromatography purification step.

9. 发明专利

AU2018200194B2 Ion exchange membrane chromatography 未知
公开(公告)号：AU2018200194B2
公开(公告)日：2019-11-14
申请号：AU2018200194
申请日：2018-01-10
申请人： GENENTECH INC
发明人： BILL JR JEROME JOSEPH , BROWN ARICK MICHAEL , DOWD CHRISTOPHER JOHN , THAYER BROOKE ELLEN
IPC分类号： C07K1/18
摘要： Abstract Methods of enhancing efficiency of downstream chromatography steps for purification of proteins comprising: (a) passing a composition comprising a polypeptide of interest and various contaminants through an ion exchange membrane, wherein the 5 polypeptide and the membrane have opposite charge, at operating conditions comprised of a buffer having a pH sufficiently distinct from the pI of the polypeptide to enhance the charge of the polypeptide and a low ionic strength effective to prevent the shielding of charges by buffer ions, which cause the membrane to bind the polypeptide and at least one contaminant, (b) overloading the ion exchange membrane such that at least one contaminant remains 10 bound to the membrane while the polypeptide of interest is primarily in the effluent; (c) collecting the effluent from the ion exchange membrane comprising the polypeptide of interest; (d) subjecting the membrane effluent comprising the polypeptide of interest to a purification step of similar charge as the previous membrane, and (e) recovering the purified polypeptide from the effluent of the charged ion exchange chromatography purification step. 9860041_1 (GHMatters) P97341.AU.1

10. 发明专利

AU2012340826A1 Purification of anti-c-met antibodies 未知
公开(公告)号：AU2012340826A1
公开(公告)日：2014-05-29
申请号：AU2012340826
申请日：2012-11-20
申请人： GENENTECH INC
发明人： PERSSON JOSEFINE , WONG MARC , BILL JR JEROME JOSEPH , BROWN ARICK MICHAEL , GIESE GLEN SCOTT , HSII JUDY FAY-CHEN , LIM AMY , RADHAMOHAN ASHA NANDINI , RODRIGUEZ MARICEL
IPC分类号： C07K16/00 , C07K16/28
摘要： Provided herein are methods of purifying anti-c-met antibodies, compositions and pharmaceutical formulations comprising purified anti-c-met antibodies, and methods of using the same.

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