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1. 发明申请

WO2010033652A1 METHOD FOR SMALL RNA ISOLATION 审中-公开
标题翻译：小RNA分离方法
公开(公告)号：WO2010033652A1
公开(公告)日：2010-03-25
申请号：PCT/US2009/057233
申请日：2009-09-17
申请人： GE HEALTHCARE BIO-SCIENCES CORP. , DHULIPALA, Rohini , CAI, Yuyang Christine , JIANG, Miao , DAR, Mubasher
发明人： DHULIPALA, Rohini , CAI, Yuyang Christine , JIANG, Miao , DAR, Mubasher
IPC分类号： C12Q1/68 , C07H21/02
CPC分类号： C12N15/111 , C12N15/1006 , C12N2310/141 , C12N2330/10
摘要： This invention relates to a simple and rapid method for the extraction and purification of small RNA from a sample solution. Accordingly, a sample is first mixed with an organic solvent to form a mixture containing the solvent. The mixture is applied to a first mineral support for large RNA to bind. The filtrate is collected which contain unbound small RNA, and is mixed with a second organic solvent to form a second mixture containing the second solvent. This second mixture is applied to a second mineral support for small RNA to bind. After a wash step, the small RNA is eluted. Also provided is a method for the isolation of large RNA, by eluting the large RNA from the first mineral support. In addition, total protein is present in the filtrate and can be isolated by a conventional method.
摘要翻译：本发明涉及从样品溶液中提取和纯化小RNA的简单而快速的方法。因此，首先将样品与有机溶剂混合以形成含有溶剂的混合物。将该混合物应用于第一种矿物载体以用于大RNA结合。收集含有未结合的小RNA的滤液，并与第二有机溶剂混合以形成含有第二溶剂的第二混合物。将该第二混合物应用于第二种矿物载体以用于小RNA结合。洗涤步骤后，小RNA被洗脱。还提供了通过从第一矿物质载体洗脱大RNA来分离大RNA的方法。此外，总蛋白存在于滤液中，可以通过常规方法分离。

2. 发明申请

WO2009018034A1 AN IMPROVED NUCLEIC ACID PURIFICATION METHOD 审中-公开
标题翻译：改进的核酸纯化方法
公开(公告)号：WO2009018034A1
公开(公告)日：2009-02-05
申请号：PCT/US2008/070837
申请日：2008-07-23
申请人： GE HEALTHCARE BIO-SCIENCES CORP. , KHAN, Manzer , DAR, Mubasher , GABRIEL, Anabela, Brito
发明人： KHAN, Manzer , DAR, Mubasher , GABRIEL, Anabela, Brito
IPC分类号： C07H21/00
CPC分类号： C07H21/00 , C12N1/06 , C12N15/1003
摘要： The invention provides an modified method for the separation of nucleic acids from cells, comprising: generating an aqueous solution containing the nucleic acid by lysing the cells with a lysis solution including SDS and salt; and separating the nucleic acids of interest from other cellular components. The improvement includes adding a non-ionic detergent in the lysis solution such that SDS is not precipitated and no heating of the solution is required prior to cellular lysis. The preferred non-ionic detergents are the polysorbate family of compound, including Tween 20. Also disclosed are composition and kit for performing the modified method.
摘要翻译：本发明提供了一种用于从细胞中分离核酸的修饰方法，包括：通过用包括SDS和盐的裂解溶液裂解细胞来产生含有该核酸的水溶液; 并将感兴趣的核酸与其它细胞成分分离。该改进包括在裂解溶液中加入非离子洗涤剂，使得SDS不沉淀，并且在细胞裂解之前不需要溶液的加热。优选的非离子洗涤剂是化合物的聚山梨醇酯族，包括吐温20.还公开了用于进行改性方法的组合物和试剂盒。

3. 发明公开

EP2324131A1 METHOD FOR SMALL RNA ISOLATION 审中-公开
标题翻译：小RNA分离方法
公开(公告)号：EP2324131A1
公开(公告)日：2011-05-25
申请号：EP09815159.0
申请日：2009-09-17
申请人： GE Healthcare Bio-Sciences Corp.
发明人： DHULIPALA, Rohini , CAI, Yuyang Christine , JIANG, Miao , DAR, Mubasher
IPC分类号： C12Q1/68 , C07H21/02
CPC分类号： C12N15/111 , C12N15/1006 , C12N2310/141 , C12N2330/10
摘要： This invention relates to a simple and rapid method for the extraction and purification of small RNA from a sample solution. Accordingly, a sample is first mixed with an organic solvent to form a mixture containing the solvent. The mixture is applied to a first mineral support for large RNA to bind. The filtrate is collected which contain unbound small RNA, and is mixed with a second organic solvent to form a second mixture containing the second solvent. This second mixture is applied to a second mineral support for small RNA to bind. After a wash step, the small RNA is eluted. Also provided is a method for the isolation of large RNA, by eluting the large RNA from the first mineral support. In addition, total protein is present in the filtrate and can be isolated by a conventional method.
摘要翻译：本发明涉及从样品溶液中提取和纯化小RNA的简单而快速的方法。因此，首先将样品与有机溶剂混合以形成含有溶剂的混合物。将混合物施加到第一种矿物质载体上以结合大RNA。收集含有未结合的小RNA的滤液，并将其与第二有机溶剂混合以形成含第二溶剂的第二混合物。将第二种混合物应用于第二种矿物支持物以结合小RNA。洗涤步骤后，小RNA被洗脱。还提供了通过从第一种矿物质载体上洗脱大RNA来分离大RNA的方法。另外，总蛋白质存在于滤液中并且可以通过常规方法分离。

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