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1. 发明专利

CA2774344C TERMINAL-PHOSPHATE-LABELED NUCLEOTIDES AND METHODS OF USE 未知
公开(公告)号：CA2774344C
公开(公告)日：2018-10-16
申请号：CA2774344
申请日：2002-08-29
申请人： GE HEALTHCARE BIO SCIENCES CORP
发明人： NELSON JOHN , FULLER CARL , SOOD ANUP , KUMAR SHIV
IPC分类号： C12Q1/68 , C07H19/10 , C07H19/20 , C07H21/00 , C12Q1/42 , C12Q1/6844 , C12Q1/6869
摘要： The present invention describes methods of detecting a nucleic acid in a sample and methods of determining the identity of a single nucleotide in a nucleic acid sequence, based on the use of terminal-phosphate-labeled nucleotides as substrates for nucleic acid polymerases. The methods provided by this invention utilize a nucleoside polyphosphate, dideoxynucleoside polyphosphate, or deoxynucleoside polyphosphate analogue which has a colorimetric dye, chemiluminescent, or fluorescent moiety, a mass tag or an electrochemical tag attached to the terminal-phosphate. When a nucleic acid polymerase uses this analogue as a substrate, an enzyme-activatable label would be present on the inorganic polyphosphate by-product of phosphoryl transfer. Cleavage of the polyphosphate product of phosphoryl transfer via phosphatase leads to a detectable change in the label attached thereon. When the polymerase assay is performed in the presence of a phosphatase, there is provided a convenient method for real- time monitoring of DNA or RNA synthesis and detection of a target nucleic acid.

2. 发明专利

AT489479T 未知
公开(公告)号：AT489479T
公开(公告)日：2010-12-15
申请号：AT03815905
申请日：2003-08-29
申请人： GE HEALTHCARE BIO SCIENCES
发明人： SOOD ANUP , KUMAR SHIV , NELSON JOHN , FULLER CARL , SEKHER ANURADHA
IPC分类号： C12Q1/68 , C12P19/34
摘要： The present invention relates generally to the use of terminal-phosphate-labeled nucleotides having three or more phosphates as substrates for nucleic acid polymerases and their use in DNA amplification. The labels employed are chemiluminescent, fluorescent, electrochemical and chromogenic moieties as well as mass tags and include those that are directly detectable, detectable after enzyme activation or feed into other processes to generate a different signal. The signal generated from the attached dyes may also be used to quantify the amount of amplification. Further provided are stabilizers that enhance the stability of terminal-phosphate labeled nucleoside polyphosphates in aqueous solutions and are useful for reducing non-enzymatic hydrolysis of these nucleotides, hence decrease background.

3. 发明专利

DE60332468D1 ANALYTNACHWEIS 未知
公开(公告)号：DE60332468D1
公开(公告)日：2010-06-17
申请号：DE60332468
申请日：2003-08-29
申请人： GE HEALTHCARE BIO SCIENCES
发明人： SOOD ANUP , KUMAR SHIV , FULLER CARL , NELSON JOHN
IPC分类号： C12N15/09 , C12P19/34 , C07H19/00 , C07H21/02 , C07H21/04 , C12Q1/02 , C12Q1/42 , C12Q1/68

4. 发明专利

DK1421213T3 Mærkede nukleosidpolyphosphater 未知
公开(公告)号：DK1421213T3
公开(公告)日：2010-06-07
申请号：DK02759495
申请日：2002-08-29
申请人： GE HEALTHCARE BIO SCIENCES
发明人： KUMAR SHIV , SOOD ANUP
IPC分类号： C12Q1/68 , G01N27/62 , C07H19/00 , C07H19/10 , C07H19/20 , C07H21/00 , C07H21/02 , C07H21/04 , C12N15/09 , C12P19/34 , C12Q1/42 , G01N21/76 , G01N21/78 , G01N33/543 , G01N33/58

5. 发明专利

DK1546354T3 Analytdetektion 未知
公开(公告)号：DK1546354T3
公开(公告)日：2010-08-16
申请号：DK03791983
申请日：2003-08-29
申请人： GE HEALTHCARE BIO SCIENCES
发明人： NELSON JOHN , SOOD ANUP , KUMAR SHIV , FULLER CARL
IPC分类号： C12N15/09 , C12Q1/68 , C07H19/00 , C07H21/02 , C07H21/04 , C12P19/34 , C12Q1/02 , C12Q1/42

6. 发明专利

DE60235383D1 未知
公开(公告)号：DE60235383D1
公开(公告)日：2010-04-01
申请号：DE60235383
申请日：2002-08-29
申请人： GE HEALTHCARE BIO SCIENCES
发明人： NELSON JOHN , FULLER CARL , SOOD ANUP , KUMAR SHIV
IPC分类号： C12Q1/68 , G01N33/53 , C07H19/00 , C07H19/10 , C07H19/20 , C07H21/00 , C07H21/02 , C07H21/04 , C12N15/09 , C12P19/34 , C12Q1/42 , C12Q1/48 , G01N33/566

7. 发明专利

AT458067T 未知
公开(公告)号：AT458067T
公开(公告)日：2010-03-15
申请号：AT02759495
申请日：2002-08-29
申请人： GE HEALTHCARE BIO SCIENCES
发明人： KUMAR SHIV , SOOD ANUP
IPC分类号： C12Q1/68 , G01N27/62 , C07H19/00 , C07H19/10 , C07H19/20 , C07H21/00 , C07H21/02 , C07H21/04 , C12N15/09 , C12P19/34 , C12Q1/42 , G01N21/76 , G01N21/78 , G01N33/543 , G01N33/58
摘要： The present invention describes new compositions of matter in the form of labeled nucleoside polyphosphates with four or more phosphates. In addition compositions of nucleoside polyphosphates with four or more phosphates that are substrates for nucleic acid polymerases with enhanced substrate properties and methods of using these nucleoside polyphosphates for nucleic acid detection, characterization and quantification are described. The compositions provided by this invention include nucleoside polyphosphate, dideoxynucleoside polyphosphate, or deoxynucleoside polyphosphate analogues which have colorimetric, chemiluminescent, or fluorescent moieties, mass tags or an electrochemical tags attached to the terminal-phosphate. When a nucleic acid polymerase uses this analogue as a substrate, an enzyme-activatable label would be present on the inorganic polyphosphate by-product of phosphoryl transfer. Removal of the polyphosphate product of phosphoryl transfer via phosphate or polyphosphate transferring enzyme leads to a detectable change in the label attached thereon. When the polymerase assay is performed in the presence of a phosphatase, there is provided a convenient method for real-time monitoring of DNA or RNA synthesis and detection of a target nucleic acid.

8. 发明专利

AU2004211588A1 Terminal-phosphate-labeled nucleotides and methods of use 未知
公开(公告)号：AU2004211588A1
公开(公告)日：2004-08-26
申请号：AU2004211588
申请日：2004-01-30
申请人： GE HEALTHCARE BIO SCIENCES CORP
发明人： FULLER CARL , KUMAR SHIV , SOOD ANUP , NELSON JOHN
IPC分类号： C07H19/10 , C07H19/20 , C07H21/00 , C12Q20060101 , C12Q1/00 , C12Q1/42 , C12Q1/68 , G01N33/52 , G01N33/58

9. 发明专利

CA2774344A1 TERMINAL-PHOSPHATE-LABELED NUCLEOTIDES AND METHODS OF USE 未知
公开(公告)号：CA2774344A1
公开(公告)日：2003-03-13
申请号：CA2774344
申请日：2002-08-29
申请人： GE HEALTHCARE BIO SCIENCES
发明人： NELSON JOHN , FULLER CARL , SOOD ANUP , KUMAR SHIV
IPC分类号： C12Q1/68 , C07H19/10 , C07H19/20 , C07H21/00 , C12Q1/42
摘要： The present invention describes methods of detecting a nucleic acid in a sample and methods of determining the identity of a single nucleotide in a nucleic acid sequence, based on the use of terminal-phosphate-labeled nucleotides as substrates for nucleic acid polymerases. The methods provided by this invention utilize a nucleoside polyphosphate, dideoxynucleoside polyphosphate, or deoxynucleoside polyphosphate analogue which has a colorimetric dye, chemiluminescent, or fluorescent moiety, a mass tag or an electrochemical tag attached to the terminal-phosphate. When a nucleic acid polymerase uses this analogue as a substrate, an enzyme-activatable label would be present on the inorganic polyphosphate by-product of phosphoryl transfer. Cleavage of the polyphosphate product of phosphoryl transfer via phosphatase leads to a detectable change in the label attached thereon. When the polymerase assay is performed in the presence of a phosphatase, there is provided a convenient method for real-time monitoring of DNA or RNA synthesis and detection of a target nucleic acid.

10. 发明专利

AT547535T 未知
公开(公告)号：AT547535T
公开(公告)日：2012-03-15
申请号：AT03791982
申请日：2003-08-29
申请人： GE HEALTHCARE BIO SCIENCES
发明人： SOOD ANUP , KUMAR SHIV , FULLER CARL , NELSON JOHN
IPC分类号： C12N15/09 , C12Q1/68 , C07H19/10 , C07H19/20 , C07H21/00 , C07H21/02 , C07H21/04 , C12N20060101 , C12P19/34

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