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1. 发明授权

US06297017B1 Categorising nucleic acids 失效
标题翻译：分类核酸
公开(公告)号：US06297017B1
公开(公告)日：2001-10-02
申请号：US09462636
申请日：2000-04-10
申请人： Günter Schmidt , Andrew Hugin Thompson
发明人： Günter Schmidt , Andrew Hugin Thompson
IPC分类号： C12Q168
CPC分类号： C12Q1/6855 , C12Q2565/518 , C12Q2563/131 , C12Q2531/119
摘要： The present invention concerns a method for characterizing one or more nucleic acids. This method involves immobilizing double-stranded nucleic acids on a solid phase support and cleaving the immobilized nucleic acids with an endonuclease, such that each cleaved nucleic acid has a double-stranded portion. The cleaved nucleic acids are then denatured to form single-stranded cleaved nucleic acids. One or more oligonucleotide sequences are then hybridized to the resulting single-stranded cleaved nucleic acid. The oligonucleotide sequences used each comprise a pre-determined recognition sequence situated such that it recognizes a sequence which was part of the double-stranded portion of the nucleic acid and a label specific to the recognition sequence. The hybridized oligonucleotide sequences are then extended along the single-stranded portion of the immobilized nucleic acid to form an extended strand which is then denatured from the immobilized strand. The immobilized nucleic acid is then characterized by identifying the size of the extended strand and the identity of the recognition sequence.
摘要翻译：本发明涉及用于表征一种或多种核酸的方法。该方法包括将固定在固相载体上的双链核酸固定并用内切核酸酶切割固定的核酸，使得每个切割的核酸具有双链部分。然后将切割的核酸变性以形成单链切割的核酸。然后将一个或多个寡核苷酸序列与所得的单链切割的核酸杂交。所使用的寡核苷酸序列包含预定的识别序列，其位置使得其识别作为核酸的双链部分的一部分的序列和识别序列特异的标记。然后将杂交的寡核苷酸序列沿着固定的核酸的单链部分延伸以形成延伸的链，然后将其从固定的链变性。然后通过鉴定扩展链的大小和识别序列的身份来表征固定的核酸。

2. 发明授权

US06699668B1 Mass label linked hybridisation probes 失效
标题翻译：大规模标签连接杂交探针
公开(公告)号：US06699668B1
公开(公告)日：2004-03-02
申请号：US09987679
申请日：2001-11-15
申请人： Günter Schmidt , Andrew Hugin Thompson , Robert Alexander Walker Johnstone
发明人： Günter Schmidt , Andrew Hugin Thompson , Robert Alexander Walker Johnstone
IPC分类号： C12Q168
CPC分类号： C07H21/00 , C12Q1/6816 , C12Q1/6837 , C12Q1/6872 , C12Q1/6874 , Y10T436/24
摘要： An array of hybridisation probes, each of which comprises a mass label linked to a known base sequence of predetermined length, wherein each mass label of the array, optionally together with the known base sequence, is relatable to that base sequence by mass spectrometry.
摘要翻译：杂交探针阵列，每个探针包含连接到已知的预定长度的碱基序列的质量标记，其中阵列的每个质量标记，任选与已知的碱基序列一起，可通过质谱法与该碱基序列相关。

3. 发明授权

US06670120B1 Categorising nucleic acid 失效
标题翻译：分类核酸
公开(公告)号：US06670120B1
公开(公告)日：2003-12-30
申请号：US09462635
申请日：2000-04-10
申请人： Günter Schmidt , Andrew Hugin Thompson
发明人： Günter Schmidt , Andrew Hugin Thompson
IPC分类号： C12Q168
CPC分类号： C12Q1/6869 , C12Q1/6855 , C12Q2525/155 , C12Q2521/301
摘要： The present invention involves a method for categorizing nucleic acid which comprises: producing a nucleic acid population by action of an endonuclease on double-stranded nucleic acid, such that each nucleic acid in the nucleic acid population has a double-stranded portion; contacting the nucleic acid population with one or more oligonucleotide sequences; and isolating nucleic acid which correctly hybridizes to an oligonucleotide sequence. In the method of the present invention, each oligonucleotide sequence has a pre-determined recognition sequence. Furthermore, the nucleic acid is categorized by its ability to correctly hybridize to oligonucleotide sequences having the recognition sequence, the recognition sequence being situated such that it recognizes a sequence in the double-stranded portion of the nucleic acid. The oligonucleotide sequence can comprise one or more different recognition sequences.
摘要翻译：本发明涉及一种用于分类核酸的方法，其包括：通过内切核酸酶在双链核酸上的作用产生核酸群体，使得核酸群体中的每个核酸具有双链部分; 使核酸群体与一个或多个寡核苷酸序列接触; 并分离与寡核苷酸序列正确杂交的核酸。在本发明的方法中，每个寡核苷酸序列具有预定的识别序列。此外，核酸通过其与具有识别序列的寡核苷酸序列正确杂交的能力分类，识别序列位于使其识别核酸双链部分中的序列。寡核苷酸序列可以包含一个或多个不同的识别序列。

4. 发明授权

US06500615B1 Identifying antisense oligonucleotide binding 失效
标题翻译：鉴定反义寡核苷酸结合
公开(公告)号：US06500615B1
公开(公告)日：2002-12-31
申请号：US09269555
申请日：1999-05-26
申请人： Günter Schmidt , Andrew Hugin Thompson
发明人： Günter Schmidt , Andrew Hugin Thompson
IPC分类号： C12Q168
CPC分类号： C12N15/113 , C12Q1/6811 , C12Q1/6837
摘要： A method for identifying an antisense oligonucleotide capable of binding to a target mRNA, which comprises contacting the target mRNA with each member of an oligonucleotide library separately under hybridization conditions, removing unhybridized material and determining which member or members hybridize; wherein the oligonucleotide library comprises a plurality of distinct nucleotide sequences of a predetermined common length, and wherein each nucleotide sequence comprises a known sequence of 4 to 8 bases and all possible combinations of the known sequence are present in the library.
摘要翻译：一种用于鉴定能够结合靶mRNA的反义寡核苷酸的方法，其包括在杂交条件下将目标mRNA与寡核苷酸文库的每个成员单独接触，除去未杂交的材料并确定哪些成员或部分杂交; 其中所述寡核苷酸文库包含多个具有预定公共长度的不同核苷酸序列，并且其中每个核苷酸序列包含4至8个碱基的已知序列，并且已知序列的所有可能组合存在于文库中。

5. 发明授权

US06287780B1 Compounds for mass spectrometry comprising nucleic acid bases and aryl ether mass markers 失效
标题翻译：用于质谱的化合物包括核酸碱基和芳基醚质量标记
公开(公告)号：US06287780B1
公开(公告)日：2001-09-11
申请号：US09581792
申请日：2000-08-11
申请人： Günter Schmidt , Andrew Hugin Thompson , Robert Alexander Walker Johnstone
发明人： Günter Schmidt , Andrew Hugin Thompson , Robert Alexander Walker Johnstone
IPC分类号： C12Q168
CPC分类号： C07H23/00 , C07H21/00 , C12Q1/6816 , C12Q1/6837 , C12Q1/6872 , C12Q1/6874 , C40B40/00
摘要： The present invention provides a compound having the following formula: N-L-M wherein N comprises one or more nucleic acid bases, L is either a direct bond between N and M or L comprises a linker moiety, and M comprises a mass marker comprising an aryl ether.
摘要翻译：本发明提供具有下式的化合物：N-L-M其中N包含一个或多个核酸碱基，L是N和M之间的直接键，或L包含连接体部分，M包括含有芳基醚的质量标记物。

6. 发明授权

US06225077B1 Method for characterizing DNA sequences 失效
标题翻译：表征DNA序列的方法
公开(公告)号：US06225077B1
公开(公告)日：2001-05-01
申请号：US09254023
申请日：1999-04-20
申请人： Günter Schmidt , Andrew Hugin Thompson
发明人： Günter Schmidt , Andrew Hugin Thompson
IPC分类号： C12Q144
CPC分类号： C12N15/1096 , C12Q1/6837 , C12Q2525/191 , C12Q2523/107
摘要： A method for characterizing cDNA, which comprises: (a) cutting a sample comprising a population of one or more cDNAs or isolated fragments thereof, each having a strand complementary to the 3′ poly-A terminus of an mRNA and bearing a tail, with a first sampling endonuclease at a first sampling site of known displacement from a reference site proximal to the tail to generate from each cDNA or isolated fragment thereof a first and second sub-fragment, each comprising a sticky end sequence of predetermined length and unknown sequence, the first sub-fragment bearing the tail; (b) sorting either the first or second sub-fragments into sub-populations according to their sticky end sequence and recording the sticky end sequence of each sub-population as the first sticky end; (c) cutting the sub-fragments of each sub-population with a second sampling endonuclease, which is the same as or different from the first sampling endonuclease, at second sampling site of known displacement from the first sampling site to generate from each sub-fragment a further sub-fragment comprising a second sticky end sequence of predetermined length and unknown sequence; and (d) determining each second sticky end sequence; wherein the aggregate length of the first and second sticky end sequences of each sub-fragment is from 6 to 10; and wherein the sequences and relative positions of the reference site and first and second sticky ends are utilized to characterize said cDNA or cDNAs.
摘要翻译：一种用于表征cDNA的方法，其包括：（a）切割包含一个或多个cDNA群体或其分离片段的样品，每个cDNA或其分离的片段具有与mRNA的3'poly-A末端互补的并具有尾部的链，在距离参考站点近尾的已知置换的第一采样位点处的第一取样内切核酸酶从每个cDNA或其分离的片段产生第一和第二子片段，每个子片段包含预定长度和未知序列的粘性末端序列，第一个带有尾巴的子片段; （b）根据第一或第二子片段的粘性末端序列将第一或第二子片段分成亚群，并记录每个子群体的粘性末端序列作为第一粘性末端; （c）在具有与第一采样内切核酸酶相同或不同于第一取样内切核酸酶的第二取样内切核酸酶切割每个亚群体的亚片段时，将包含预定长度和未知序列的第二粘性末端序列的另外的子片段片段化; 和（d）确定每个第二粘性末端序列; 其中每个子片段的第一和第二粘性末端序列的聚合长度为6至10; 并且其中使用参考部位和第一和第二粘性末端的序列和相对位置来表征所述cDNA或cDNA。

7. 发明授权

US07056659B1 Characterizing nucleic acids 失效
标题翻译：表征核酸
公开(公告)号：US07056659B1
公开(公告)日：2006-06-06
申请号：US09462408
申请日：1998-07-13
申请人： Günter Schmidt , Andrew Hugin Thompson
发明人： Günter Schmidt , Andrew Hugin Thompson
IPC分类号： C12Q1/68 , C12P19/34 , C07H21/04 , B01D57/02
CPC分类号： C12Q1/6872 , C12Q2563/167 , C12Q2535/101
摘要： A method for characterizing DNA, which comprises: (i) providing a population of DNA fragments, each fragment having cleavably attached thereto a mass label for identifying a feature of that fragment; (ii) separating the fragments on the basis of their length; (iii) cleaving each fragment in a mass spectrometer to release its mass label; and (iv) determining each mass label by mass spectrometry to relate the feature of each fragment to the length of the fragment.
摘要翻译：一种用于表征DNA的方法，其包括：（i）提供DNA片段群，每个片段与其可切割地连接有用于鉴定该片段特征的质量标签; （ii）根据其长度分离碎片; （iii）在质谱仪中切割每个片段以释放其质量标签; 和（iv）通过质谱法确定每个质量标签以将每个片段的特征与片段的长度相关联。

8. 发明授权

US06312904B1 Characterizing nucleic acid 失效
标题翻译：表征核酸
公开(公告)号：US06312904B1
公开(公告)日：2001-11-06
申请号：US09462600
申请日：2000-04-10
申请人： Günter Schmidt , Andrew Hugin Thompson
发明人： Günter Schmidt , Andrew Hugin Thompson
IPC分类号： C12Q168
CPC分类号： C12Q1/6855 , C12Q1/6874 , C12Q2535/101
摘要： The present invention involves a method for characterizing nucleic acid which comprises generating Sanger ladder nucleic acid fragments from a plurality of nucleic acid templates present in the same reaction zone, wherein at least one terminating base is present in the reaction zone. Prior to generating nucleic acid fragments, a labeled primer nucleotide or oligonucleotide is hybridized to each template. The label on each primer is specific to the template to which that primer hybridizes, thereby allowing for identification of the template. The method of the present invention further comprises identifying the length of each nucleic acid fragment produced, the template from which the fragment is derived and the terminating base of the fragment.
摘要翻译：本发明涉及用于表征核酸的方法，其包括从存在于相同反应区中的多个核酸模板生成Sanger梯形核酸片段，其中在反应区中存在至少一个终止碱基。在产生核酸片段之前，将标记的引物核苷酸或寡核苷酸与每个模板杂交。每个引物上的标记对于该引物杂交的模板是特异的，从而允许鉴定模板。本发明的方法还包括鉴定产生的每个核酸片段的长度，衍生片段的模板和片段的终止碱基。

9. 发明授权

US06846679B1 Characterizing polypeptides through cleavage and mass spectrometry 失效
标题翻译：通过切割和质谱鉴定多肽
公开(公告)号：US06846679B1
公开(公告)日：2005-01-25
申请号：US09806564
申请日：1999-10-01
申请人： Gunter Schmidt , Andrew Hugin Thompson
发明人： Gunter Schmidt , Andrew Hugin Thompson
IPC分类号： G01N27/62 , C07K1/12 , C12Q1/02 , G01N33/68 , G01N33/50
CPC分类号： G01N33/6848 , C07K1/12 , G01N33/6842 , Y10T436/24
摘要： Provided is a method for characterizing a polypeptide or a population of polypeptides, which method comprises: (a) contacting a sample comprising one or more polypeptides with a first cleavage agent to generate polypeptide fragments; (b) isolating one or more polypeptide fragments, each fragment comprising the N-terminus or the C-terminus of the polypeptide from which it was fragmented; (c) identifying the isolated fragments by mass spectrometry; (d) repeating steps (a)-(c) on the sample using a second cleavage agent that cleaves at a difference site from the first cleavage agent; and (e) characterizing the one or more polypeptides in the sample from the fragments identified in steps (c) and (d).
摘要翻译：提供了表征多肽或多肽群体的方法，该方法包括：（a）使包含一种或多种多肽的样品与第一切割剂接触以产生多肽片段; （b）分离一个或多个多肽片段，每个片段包含多肽片段的N末端或C末端; （c）通过质谱鉴定分离的片段; （d）使用在与第一切割剂的差异位点切割的第二切割剂对样品重复步骤（a） - （c）; 和（e）从步骤（c）和（d）中鉴定的片段表征样品中的一种或多种多肽。

10. 发明授权

US06391560B1 Screening for functional antisense agents 失效
标题翻译：筛选功能性反义试剂
公开(公告)号：US06391560B1
公开(公告)日：2002-05-21
申请号：US09623390
申请日：2001-03-26
申请人： Gunter Schmidt , Andrew Hugin Thompson
发明人： Gunter Schmidt , Andrew Hugin Thompson
IPC分类号： C12Q168
CPC分类号： C12Q1/6811 , C12Q1/6837 , C12Q2521/301
摘要： Provided is a method for identifying a functional antisense agent, which method comprises contacting an RNA with an oligonucleotide probe to form a duplex-containing RNA, contacting the duplex-containing RNA with an RNAase to cleave the duplex-containing RNA, and measuring the kinetics of cleavage.
摘要翻译：提供了用于鉴定功能性反义剂的方法，该方法包括使RNA与寡核苷酸探针接触以形成含双链体的RNA，使含双链体的RNA与RNA酶接触以切割含双链体的RNA，并测量动力学的切割。

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