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1. 发明授权

US5003054A Ouabain triacetate derivative compounds 失效
标题翻译：哇巴因三醋酸酯衍生物化合物
公开(公告)号：US5003054A
公开(公告)日：1991-03-26
申请号：US235267
申请日：1988-08-23
申请人： Frank C. Grenier , Terry A. Pry , Lawrence Kolaczkowski
发明人： Frank C. Grenier , Terry A. Pry , Lawrence Kolaczkowski
IPC分类号： C07J19/00 , G01N33/94
CPC分类号： G01N33/9453 , C07J19/005
摘要： A method for performing a diagnostic immunoassay by solid phase separation for digoxin. To a reaction mixture of a test sample and labeled anti-digoxin antibody, which forms a complex of any digoxin present in the test sample, is added a solid phase material having an immobilized ouabain triacetate derivative compound capable of binding any excess labeled antibody. The solid phase material is chosen to rapidly settle whereby a solid and liquid phase is formed. The liquid phase can then be extracted to measure the amount of digoxin-labeled antibody present therein. Ouabain triacetate derivative compounds possess sufficient affinity for anti-digoxin antibodies, and are therefore useful in a solid phase separation based digoxin immunoassay for settling out such antibodies without contributing to undesired background interference. These compounds are also less deleterious to assay performance because of low recognition when leached from the solid phase matrix thereby providing solid phase material with extended shelf life.
摘要翻译：通过固相分离进行地高辛的诊断免疫测定的方法。向测试样品中形成测试样品中存在的任何地高辛的复合物的标记的抗地高辛抗体的反应混合物中加入具有能够结合任何过量标记抗体的固定化的哇巴因三乙酸酯衍生物化合物的固相材料。选择固相材料快速沉降，从而形成固相和液相。然后可以提取液相以测量其中存在的地高辛标记的抗体的量。哇巴因三乙酸酯衍生物化合物对抗地高辛抗体具有足够的亲和力，因此可用于基于固相分离的地高辛免疫测定，用于沉淀此类抗体，而不会造成不需要的背景干扰。这些化合物对于测定性能也不太有害，因为当从固相基质中浸出时，识别率低，从而提供具有延长保质期的固相材料。

2. 发明授权

US4788136A Diagnostic immunoassay by solid phase separation for digoxin 失效
标题翻译：通过固相分离用于地高辛的诊断免疫测定
公开(公告)号：US4788136A
公开(公告)日：1988-11-29
申请号：US35673
申请日：1987-04-07
申请人： Frank C. Grenier , Terry A. Pry , Lawrence Kolaczkowski
发明人： Frank C. Grenier , Terry A. Pry , Lawrence Kolaczkowski
IPC分类号： C07J19/00 , G01N33/53 , G01N33/94 , G01N33/537 , G01N33/552 , G01N33/563
CPC分类号： G01N33/9453 , C07J19/005 , Y10S435/971 , Y10S436/824 , Y10T436/11 , Y10T436/25375
摘要： A method for performing a diagnostic immunoassay by solid phase separation for digoxin. To a reaction mixture of a test sample and labeled anti-digoxin antibody, which forms a complex of any digoxin present in the test sample, is added a solid phase material having an immobilized ouabain triacetate derivative compound capable of binding any excess labeled antibody. The solid phase material is chosen to rapidly settle whereby a solid and liquid phase is formed. The liquid phase can then be extracted to measure the amount of digoxin-labeled antibody present therein. Ouabain triacetate derivative compounds possess sufficient affinity for anti-digoxin antibodies, and are therefore useful in a solid phase separation based digoxin immunoassay for settling out such antibodies without contributing to undesired background interference. These compounds are also less deleterious to assay performance because of low recognition when leached from the solid phase matrix thereby providing solid phase material with extended shelf life.

3. 发明授权

US5064541A Devices and methods for the collection of a predetermined volume of plasma or serum 失效
标题翻译：用于收集预定体积的血浆或血清的装置和方法
公开(公告)号：US5064541A
公开(公告)日：1991-11-12
申请号：US499864
申请日：1990-03-27
申请人： Tzyy-Wen Jeng , Kristin D. Elmore , Gary M. Oosta , Terry A. Pry
发明人： Tzyy-Wen Jeng , Kristin D. Elmore , Gary M. Oosta , Terry A. Pry
IPC分类号： B01D39/00 , B01D39/16 , B01D39/18 , B01D39/20 , B01L3/00 , G01N33/49 , G01N33/52 , G01N33/53
CPC分类号： B01L3/508 , B01D39/00 , B01D39/163 , B01D39/1661 , B01D39/18 , B01D39/201 , B01D39/2034 , B01D39/2075 , B01L3/5023 , G01N33/491 , G01N33/526 , G01N33/5302 , B01L2200/12 , B01L2300/0681 , B01L2300/0825 , B01L2300/087 , B01L2400/0406 , Y10T436/25375
摘要： A device and method for permitting the separation of plasma or serum from whole blood. The device comprises a matrix of hydrophilic sintered porous material to which at least one red blood cell agglutinating agent has been applied. According to a first method of using the device, a sample of whole blood is applied to a first end of the matrix and the red blood cells within the sample come in contact with the agglutinating agents present in the matrix. The blood cells agglutinate, and are entrapped in the interstices of the matrix, while substantially blood-cell-free serum or plasma accumulates near the outlet of the device. A filter means in liquid receiving relationship with the matrix functions to wick the serum of plasma from the matrix.According to an alternative aspect of the invention, a filter means in liquid receiving relationship with the outlet of the matrix functions to retain any blood cells which pass through the matrix as the filter means wicks the plasma or serum from the matrix. Additional agglutinating agents may be incorporated within the filter means to facilitate retention of blood cells which pass through the matrix.Another aspect of the present invention involves a device for the measurement of a serum or plasma sample, utilizing a matrix of sintered porous material, such as sintered glass, sintered steel, sintered ceramics, sintered plastics, and equivalents thereof. The matrix is characterized by a reproducible fluid uptake capacity proportional to the fixed dimensions of said matrix, a minimal reactivity with plasma or serum components, and a hydrophilic internal surface which enables the matrix to collect and retain a predetermined volume of sample for analysis.
摘要翻译：允许血浆或血清从全血分离的装置和方法。该装置包括已经施加至少一种红细胞凝集剂的亲水性烧结多孔材料的基质。根据使用该装置的第一种方法，将全血样品施加到基质的第一端，并且样品内的红细胞与存在于基质中的凝集剂接触。血细胞凝集，并被截留在基质的空隙中，而基本上无血细胞的血清或血浆聚集在装置的出口附近。与基质液体接收关系的过滤装置用于从基质中吸收血浆的血清。根据本发明的另一方面，与基质的出口液体接收关系的过滤器装置用于保持通过基质的任何血细胞，因为过滤器装置将血浆或血清从基质中吸出。可以在过滤器装置内并入额外的凝集剂以促进通过基质的血细胞的保留。本发明的另一方面涉及一种利用烧结多孔材料如烧结玻璃，烧结钢，烧结陶瓷，烧结塑料及其等同物的基质来测量血清或血浆样品的装置。该基质的特征在于与所述基质的固定尺寸成比例的可再现的流体吸收能力，与血浆或血清组分的最小反应性，以及亲水性内表面，其使基质收集并保留预定体积的样品用于分析。

4. 发明授权

US5681754A Method for improving the performance of an immunoreagent in an immunoassay 失效
标题翻译：在免疫测定中提高免疫反应性能的方法
公开(公告)号：US5681754A
公开(公告)日：1997-10-28
申请号：US657578
申请日：1996-06-07
申请人： Mark R. Pope , Peter J. Tarcha , David R. Mees , Mary K. Joseph , Terry A. Pry , C. Brent Putman , Daniel D. Subotich
发明人： Mark R. Pope , Peter J. Tarcha , David R. Mees , Mary K. Joseph , Terry A. Pry , C. Brent Putman , Daniel D. Subotich
IPC分类号： G01N33/543 , G01N33/547 , C12Q1/00 , G01N33/544 , G01N33/546
CPC分类号： G01N33/54393 , G01N33/54353 , Y10S435/962 , Y10S435/963 , Y10S436/81 , Y10S436/823
摘要： A method for increasing the binding activity of specific binding members bound to a solid phase material, e.g., a particle, that has been sterically stabilized. This increase in binding activity is brought about by degrading a steric stabilizer on the surface of the solid phase material. The method involves both immobilizing a specific binding member on the surface of a solid phase material and degrading a steric stabilizer on the surface of that solid phase material. In the preferred embodiment, the method involves the immobilization of a specific binding member on the surface of the sterically stabilized solid phase material, with subsequent degradation of the steric stabilizer.
摘要翻译：用于增加与已经空间稳定的固相物质例如颗粒结合的特异性结合成员的结合活性的方法。结合活性的这种增加是通过降解固相材料表面上的空间稳定剂引起的。该方法包括将特定的结合构件固定在固相材料的表面上并降解固相材料表面上的空间稳定剂。在优选的实施方案中，该方法包括将特定结合成分固定在空间稳定的固相材料的表面上，随后空间稳定剂的降解。

5. 发明授权

US5229268A Method for diagnostic immunoassay by solid phase separation 失效
标题翻译：通过固相分离进行诊断免疫测定的方法
公开(公告)号：US5229268A
公开(公告)日：1993-07-20
申请号：US172503
申请日：1987-11-24
申请人： Terry A. Pry , Edward N. Granados , Philip M. Hill
发明人： Terry A. Pry , Edward N. Granados , Philip M. Hill
IPC分类号： G01N33/538
CPC分类号： G01N33/538 , Y10S436/824 , Y10T436/25375
摘要： A method for performing a diagnostic immunoassay by a solid phase separation. To a reaction mixture of a test sample and labeled antibody, which forms a complex of any analyte present in the test sample, is added a solid phase material having a compound capable of binding any excess labeled antibody. The solid phase material is chosen to rapidly settle whereby a solid and liquid phase is formed. The liquid phase can then be extracted to measure the amount of analyte-labeled antibody present therein.
摘要翻译：用于通过固相分离进行诊断免疫测定的方法。向形成测试样品中存在的任何分析物的复合物的测试样品和标记抗体的反应混合物中加入具有能够结合任何过量标记抗体的化合物的固相材料。选择固相材料快速沉降，从而形成固相和液相。然后可以提取液相以测量其中存在的分析物标记的抗体的量。

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